Standard name
Human Ortholog
Description Intra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.98 0.95 0.97 0.94 0.91 0.81 0.84 – 0.74 0.74 0.72 0.99 0.99 0.99 0.94 0.98 0.89 1.0 0.94 0.93 0.89 0.9 0.87
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0.06 0.08 0.16 0.1 – 0.16 0.24 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0.07 0.09 0.09 – 0.17 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1
Bud – 0 0 0 3 2 6 3 – 7 4 12 3 0 0 0 0 0 0 0 0 1 10 7
Bud Neck – 0 0 0 1 1 0 0 – 1 2 0 2 0 0 0 1 0 0 0 0 0 1 0
Bud Site – 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 3 0 1 2 6 3 14 – 8 6 5 0 2 2 4 2 4 0 0 0 0 0 0
Cytoplasm – 177 69 250 476 450 549 686 – 532 267 357 383 600 600 169 119 103 24 167 82 177 432 452
Endoplasmic Reticulum – 3 1 0 0 1 8 2 – 4 1 1 2 6 7 7 3 5 0 0 0 2 2 4
Endosome – 0 0 0 4 1 0 1 – 0 2 0 0 1 1 2 0 1 0 0 0 2 1 7
Golgi – 0 0 0 0 0 0 0 – 0 1 0 0 1 0 2 0 1 0 1 0 1 1 1
Mitochondria – 0 1 6 31 40 105 82 – 115 87 142 0 0 0 5 0 5 0 0 0 1 1 9
Nucleus – 1 1 0 3 4 13 12 – 13 10 9 5 1 1 1 3 1 0 1 0 1 4 1
Nuclear Periphery – 0 0 0 1 0 2 1 – 4 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus – 0 1 0 0 0 0 0 – 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole – 0 0 0 0 0 2 4 – 4 1 3 0 0 0 1 0 0 0 0 0 0 7 7
Vac/Vac Membrane – 1 1 10 6 34 59 75 – 125 37 35 1 3 3 2 4 5 0 1 1 7 11 14
Unique Cell Count 180 73 259 504 494 674 818 723 363 495 387 608 609 179 122 116 25 177 88 199 483 518
Labelled Cell Count 185 74 267 527 539 747 880 813 419 566 396 614 614 194 132 127 25 177 88 199 483 518


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 8.8 6.3 7.7 6.6 6.8 5.8 6.8 – 6.5 6.9 6.0 8.2 8.4 8.4 8.6 8.6 10.1 8.9 8.6 8.8
Std Deviation (1e-4) – 1.3 1.5 2.8 1.9 1.7 1.7 1.9 – 1.8 4.0 1.9 2.0 1.8 1.8 3.1 3.3 3.7 1.6 1.9 1.8
Intensity Change (Log2) – – – 0.3 0.09 0.12 -0.1 0.11 – 0.06 0.13 -0.06 0.38 0.43 0.43 0.46 0.46 0.69 0.5 0.46 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0 -1.0 -2.8 -2.4 – -4.0 -3.9 -4.1 2.7 2.6 2.4 0 1.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.1 3.3 2.4 – 3.4 4.4 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.2 2.3 – 3.6 2.4 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4404 6.3526 5.4934 5.4066 4.721 5.564 3.2409 5.0579 4.8406 3.6966 3.3121 4.576 3.1446 4.4112 4.163 4.2698 2.2817 3.9535
Actin 0.025 0.0019 0.0019 0.0007 0.0011 0.0003 0.023 0.0021 0.0012 0.0277 0.0428 0.0019 0.0118 0.0015 0.0068 0.0004 0.0058 0.003
Bud 0.0007 0.0003 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0017 0.0003 0.0002 0.0001 0.0004 0.0002 0.0006 0.0001 0.0002 0.0001
Bud Neck 0.0051 0.0004 0.0004 0.0004 0.0007 0.0008 0.0007 0.0002 0.0004 0.0008 0.0006 0.0007 0.0023 0.0009 0.0008 0.0005 0.0021 0.0008
Bud Periphery 0.0007 0.0001 0 0 0 0 0.0004 0 0.0008 0.0002 0.0002 0.0001 0.0004 0.0001 0.0009 0 0.0001 0
Bud Site 0.004 0.0098 0.0002 0.0001 0.0001 0 0.0025 0.0006 0.008 0.0006 0.0009 0.0001 0.0008 0.0063 0.0032 0.0001 0.0026 0.0001
Cell Periphery 0.0003 0.0001 0 0 0 0 0.0001 0.0003 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0003 0
Cytoplasm 0.8677 0.9469 0.9543 0.9626 0.955 0.9521 0.755 0.9421 0.9308 0.8864 0.7736 0.9474 0.9159 0.9688 0.9268 0.9763 0.9077 0.9468
Cytoplasmic Foci 0.0189 0.0052 0.0028 0.0032 0.0052 0.0044 0.0514 0.0014 0.0036 0.0426 0.0198 0.0056 0.0089 0.0039 0.007 0.0034 0.0071 0.009
Eisosomes 0.0002 0 0 0 0 0 0.0008 0.0002 0 0 0.0018 0 0.0001 0 0.0001 0 0.0005 0.0001
Endoplasmic Reticulum 0.0057 0.0017 0.0027 0.0033 0.0096 0.0023 0.0047 0.0011 0.0032 0.001 0.0035 0.0018 0.0049 0.0019 0.0024 0.0023 0.0049 0.0029
Endosome 0.0128 0.0051 0.0011 0.0014 0.0029 0.0066 0.0088 0.0003 0.0058 0.0056 0.0114 0.0027 0.0079 0.002 0.0099 0.0016 0.005 0.0115
Golgi 0.0026 0.0006 0.0002 0.0001 0.0002 0.0005 0.0036 0.0001 0.0003 0.0034 0.0119 0.0016 0.004 0.0017 0.0033 0.0001 0.0011 0.001
Lipid Particles 0.0024 0.0011 0 0.0001 0.0001 0 0.0051 0 0.0001 0.0016 0.0289 0.0064 0.0072 0.0007 0.0061 0 0.0019 0.0012
Mitochondria 0.0015 0.0002 0.0001 0.0001 0.0002 0.0002 0.0291 0 0.0004 0.0006 0.0083 0.0021 0.0049 0.0001 0.0032 0.0001 0.0005 0.0004
None 0.0266 0.0121 0.0177 0.012 0.0118 0.0117 0.0684 0.0487 0.0306 0.0073 0.0292 0.0112 0.0145 0.0066 0.0086 0.0033 0.0486 0.0048
Nuclear Periphery 0.0038 0.0016 0.002 0.0019 0.0026 0.0017 0.0042 0.0005 0.0033 0.0006 0.0042 0.0009 0.0018 0.0008 0.0031 0.0021 0.002 0.0034
Nucleolus 0.0009 0 0 0 0 0 0.0006 0 0.0001 0 0.0008 0 0.0002 0 0.0003 0 0.0004 0.0001
Nucleus 0.0099 0.0099 0.015 0.0124 0.0085 0.0177 0.0062 0.0019 0.0072 0.0041 0.005 0.008 0.0045 0.0029 0.0051 0.0071 0.0042 0.0099
Peroxisomes 0.0026 0.0005 0.0001 0.0001 0.0001 0.0001 0.014 0 0.0001 0.0155 0.0486 0.0082 0.0061 0.0003 0.0056 0.0001 0.0005 0.0005
Punctate Nuclear 0.0055 0.0018 0.0008 0.0012 0.0012 0.0009 0.0198 0.0002 0.0009 0.0015 0.0068 0.0007 0.0015 0.0008 0.0051 0.0021 0.0033 0.0037
Vacuole 0.0027 0.0004 0.0004 0.0003 0.0005 0.0004 0.0008 0.0002 0.001 0.0002 0.0008 0.0003 0.0014 0.0003 0.0007 0.0004 0.0009 0.0005
Vacuole Periphery 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0005 0.0001 0.0003 0 0.0003 0.0001 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.9011 23.4851 14.9374 32.0322 17.0311 33.759 26.29 30.9089 23.3049 33.1224
Translational Efficiency 0.9071 1.6949 2.1431 1.3669 2.7374 1.3719 1.6672 1.7918 2.0194 1.6316

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1028 1262 217 1051 1548 1450 1877 1246 2576 2712 2094 2297

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 733.66 967.53 1167.71 1027.90 809.41 917.02 1028.05 1065.86 779.18 940.52 1042.52 1048.49
Standard Deviation 92.24 146.97 134.11 175.48 133.53 132.75 171.45 158.41 124.44 141.80 173.27 167.51
Intensity Change Log 2 — 0.399195 0.670498 0.486516 — 0.180083 0.344968 0.397075 — 0.288417 0.508917 0.440293

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000231 0.000307 0.000892 0.004386 0.000113 0.000538 0.000520 0.005297 0.000160 0.000430 0.000559 0.004880
Bud Neck 0.004189 0.001845 0.002837 0.005777 0.002280 0.004147 0.001789 0.004820 0.003042 0.003076 0.001897 0.005258
Bud Site 0.011306 0.007879 0.006020 0.033601 0.004524 0.009629 0.004966 0.033161 0.007231 0.008815 0.005075 0.033362
Cell Periphery 0.000276 0.000086 0.000259 0.000160 0.000081 0.000067 0.000063 0.000310 0.000159 0.000076 0.000084 0.000241
Cytoplasm 0.658532* 0.769005* 0.438668* 0.641339* 0.730267* 0.716340* 0.710714* 0.610531* 0.701640* 0.740847* 0.682522* 0.624627*
Cytoplasmic Foci 0.194872* 0.071139 0.012489 0.011361 0.091254 0.091224 0.005868 0.026263 0.132605 0.081877 0.006554 0.019444
Eisosomes 0.000073 0.000019 0.000050 0.000017 0.000026 0.000026 0.000009 0.000037 0.000045 0.000023 0.000013 0.000028
Endoplasmic Reticulum 0.001942 0.001425 0.005553 0.001162 0.000919 0.001006 0.000588 0.001597 0.001327 0.001201 0.001103 0.001398
Endosome 0.007185 0.005979 0.006141 0.005736 0.006267 0.011551 0.000851 0.014116 0.006633 0.008958 0.001400 0.010282
Golgi 0.002776 0.001265 0.000251 0.003174 0.000947 0.003273 0.000243 0.010831 0.001677 0.002338 0.000244 0.007328
Lipid Particles 0.002650 0.001204 0.003103 0.001016 0.001282 0.001068 0.000382 0.001104 0.001828 0.001132 0.000664 0.001063
Mitochondria 0.003068 0.000605 0.000413 0.001364 0.001142 0.002603 0.000244 0.006239 0.001911 0.001674 0.000262 0.004009
Mitotic Spindle 0.001129 0.002950 0.011144 0.057963 0.000934 0.010330 0.007225 0.046868 0.001012 0.006896 0.007631 0.051945
None 0.002420 0.004173 0.003594 0.009837 0.006690 0.002947 0.010391 0.007732 0.004986 0.003518 0.009687 0.008695
Nuclear Periphery 0.000151 0.000233 0.001895 0.000598 0.000396 0.000212 0.000491 0.000584 0.000298 0.000222 0.000637 0.000591
Nuclear Periphery Foci 0.001231 0.003540 0.013039 0.002817 0.002376 0.001190 0.001414 0.001849 0.001919 0.002284 0.002618 0.002292
Nucleolus 0.000561 0.000307 0.000207 0.001276 0.000796 0.000587 0.000130 0.000582 0.000702 0.000457 0.000138 0.000900
Nucleus 0.065148 0.064746 0.298714* 0.135680* 0.074406 0.051448 0.210256* 0.100542 0.070712 0.057636 0.219423* 0.116620
Peroxisomes 0.001287 0.000765 0.000281 0.001165 0.000698 0.002629 0.000421 0.006864 0.000933 0.001762 0.000406 0.004256
Vacuole 0.038076 0.061604 0.194173 0.079633 0.074018 0.087792 0.042409 0.114009 0.059675 0.075606 0.058136 0.098280
Vacuole Periphery 0.002898 0.000922 0.000280 0.001938 0.000582 0.001392 0.001025 0.006663 0.001506 0.001173 0.000947 0.004501

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.40 -10.03 -2.46 -2.28 -0.55 -1.80 -1.45 -2.00 -1.18 -1.18 -2.12 -1.70 -3.07 -2.25 -1.66
Bud Neck 5.13 0.49 0.54 -3.99 0.16 -3.61 0.10 -1.71 2.19 -1.88 -0.22 2.18 -0.94 -0.65 -2.89
Bud Site 2.85 2.17 -1.45 -3.33 -2.91 -4.04 0.40 -4.79 -1.90 -4.91 -1.80 3.75 -4.85 -3.54 -6.76
Cell Periphery 3.77 -0.45 2.66 -4.98 7.55 1.71 1.27 -1.25 -1.66 -1.51 3.99 3.03 0.97 -2.88 -1.85
Cytoplasm -10.22 17.74 15.15 26.02 -8.00 2.20 15.37 20.69 17.41 8.43 -4.66 17.28 25.92 30.05 8.74
Cytoplasmic Foci 19.16 31.22 31.90 20.84 0.91 0.21 25.98 22.35 21.48 -8.14 13.20 39.24 37.00 29.54 -8.06
Eisosomes 12.68 1.75 10.89 -4.88 7.31 -0.06 6.87 -0.75 -0.67 -5.37 9.73 12.28 6.92 -2.69 -5.26
Endoplasmic Reticulum 1.22 -7.40 2.50 0.38 9.85 -0.12 0.64 -0.86 -0.12 -1.69 0.53 -0.55 1.54 0.14 3.14
Endosome 1.84 1.12 8.74 5.32 6.44 -4.72 10.19 2.96 6.55 -3.79 -3.03 13.82 6.72 8.23 -3.21
Golgi 3.64 8.41 4.60 1.01 -1.13 -2.78 2.23 -1.49 1.04 -2.80 -1.10 6.23 0.52 1.33 -3.15
Lipid Particles 4.70 -2.70 3.75 -2.37 6.10 1.11 3.64 -0.39 -1.76 -5.50 4.23 5.52 2.38 -2.92 -4.91
Mitochondria 3.13 3.42 3.28 0.19 -0.88 -1.32 1.28 -0.31 1.09 -1.81 0.52 3.27 1.64 1.09 -2.04
Mitotic Spindle -1.46 -2.48 -8.34 -7.60 -2.89 -4.47 -6.44 -7.58 -3.64 -4.94 -4.68 -6.91 -11.26 -7.70 -7.25
None -2.41 -0.76 -8.47 -4.76 -7.27 3.34 -2.43 0.31 -4.82 5.03 2.11 -5.22 -2.98 -6.75 3.00
Nuclear Periphery -2.11 -17.31 -19.81 -15.95 8.46 0.88 -3.77 -3.16 -16.11 1.91 0.53 -8.19 -7.22 -22.44 2.30
Nuclear Periphery Foci -4.97 -7.71 -8.29 0.59 6.29 4.55 2.29 -0.20 -6.46 -3.36 -1.68 -4.15 -4.85 -1.91 0.42
Nucleolus 1.02 1.62 0.17 -0.97 -1.70 0.64 5.86 3.94 1.66 -2.01 1.29 4.90 2.12 0.72 -2.60
Nucleus -0.55 -13.45 -18.51 -18.72 6.85 7.07 -35.13 -13.03 -19.38 20.28 4.79 -39.67 -21.99 -26.72 20.37
Peroxisomes 2.98 5.93 5.12 3.27 -0.49 -2.83 1.89 -1.38 1.16 -1.89 -2.25 3.48 -0.30 1.48 -1.83
Vacuole -8.31 -14.36 -26.06 -21.47 1.86 -4.13 -5.24 -22.30 -18.95 -18.49 -6.95 -13.68 -33.32 -28.48 -19.67
Vacuole Periphery 2.25 2.98 2.76 1.60 -1.12 -2.90 0.62 -1.31 0.08 -1.47 1.03 2.58 1.35 0.64 -1.32
Standard name
Human Ortholog
Description Intra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sft1

Sft1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sft1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available