| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; mutation is functionally complemented by human ARPC4 |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 37 | – | – | – | – | – | – | – | – | – | – | – | – | – | 16 | – | – | – | 75 | 0 | 0 | 0 | 1 |
| Bud | – | 7 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | – | – | – | 15 | 0 | 0 | 0 | 0 |
| Bud Neck | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 3 | 0 | 0 | 0 | 0 |
| Bud Site | – | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | – | – | – | – | – | – | – | – |
| Cell Periphery | – | 11 | – | – | – | – | – | – | – | – | – | – | – | – | – | 7 | – | – | – | 1 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 10 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | – | – | – | 3 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | – | 7 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | – | – | – | 2 | 0 | 0 | 0 | 0 |
| Endosome | – | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | – | – | – | 6 | 0 | 0 | 0 | 1 |
| Golgi | – | 10 | – | – | – | – | – | – | – | – | – | – | – | – | – | 10 | – | – | – | 6 | 0 | 0 | 0 | 0 |
| Mitochondria | – | 46 | – | – | – | – | – | – | – | – | – | – | – | – | – | 7 | – | – | – | 3 | 0 | 0 | 0 | 0 |
| Nucleus | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 5 | – | – | – | – | – | – | – | – | – | – | – | – | – | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 3 | – | – | – | 1 | 0 | 0 | 0 | 0 |
| Unique Cell Count | 87 | 35 | 121 | 1 | 2 | 1 | 5 | |||||||||||||||||
| Labelled Cell Count | 135 | 58 | 121 | 1 | 2 | 1 | 5 | |||||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 9.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | 11.6 | – | – | – | – | – |
| Std Deviation (1e-4) | – | 2.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
