Standard name
Human Ortholog
Description Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.05 0.05 0.14 0 0.11 0.08 0.06 0.08 0.06
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0.17 0.06 0 0 0 – 0.08 0 0 0 – 0 0 0 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.06 0.12 0.33 0.21 0.4 0.07 0 – 0 0.09 0 0 – 0.38 0.31 0.59 0.49 0.48 0.15 0.18 0.11 0.29 0.32 0.31
Mitochondria 0.96 0.92 0.38 0.81 0.66 0.84 0.83 – 0.72 0.84 0.93 0.88 – 0.7 0.7 0.16 0.1 0.09 0.71 0.62 0.56 0.53 0.49 0.47
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.05 0.08 0.06 0.1 0.12 0.12 – 0.18 0.13 0.1 0.12 – 0.05 0 0.12 0.14 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0.17 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0 0.08 0.07 0.15 0.11 0.06 – 0 0 0.05 0 – 0.15 0.1 0.11 0.15 0.16 0 0 0 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 2 3 1 0 – 0 1 0 1 – 6 5 8 7 6 3 9 5 4 10 10
Bud 0 0 0 1 0 2 2 – 1 3 3 2 – 0 0 0 0 0 0 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 0 0 0 – 0 0 0 0 – 1 2 7 4 3 0 0 0 0 0 0
Cytoplasm 0 0 4 5 3 4 4 – 6 1 4 2 – 7 6 6 9 2 0 0 1 0 1 2
Endoplasmic Reticulum 0 2 0 0 1 0 0 – 1 0 0 0 – 0 0 3 3 0 0 1 0 0 0 1
Endosome 0 0 1 0 0 0 2 – 1 2 0 1 – 0 0 0 2 1 0 0 1 0 1 0
Golgi 6 9 8 19 48 7 4 – 0 15 0 4 – 63 45 94 68 21 16 16 6 21 41 48
Mitochondria 100 71 9 73 80 89 118 – 52 132 146 149 – 117 101 25 14 4 77 56 34 40 64 73
Nucleus 1 0 0 0 0 1 1 – 1 0 0 1 – 1 0 1 2 0 0 0 0 0 0 1
Nuclear Periphery 2 0 0 1 0 1 7 – 1 5 3 5 – 2 1 0 0 0 0 0 0 0 1 0
Nucleolus 17 4 2 5 12 13 17 – 13 20 16 20 – 8 5 20 20 5 4 1 2 2 1 1
Peroxisomes 0 0 4 1 1 1 3 – 1 7 0 4 – 0 1 3 5 1 1 0 3 1 2 1
SpindlePole 0 0 1 0 1 0 1 – 0 3 2 1 – 0 0 1 0 2 1 0 2 0 0 0
Vac/Vac Membrane 10 2 2 6 18 12 9 – 1 7 8 6 – 25 14 17 21 7 3 1 0 4 5 9
Unique Cell Count 104 77 24 90 121 106 143 72 158 157 169 168 144 160 140 44 109 91 62 77 132 157
Labelled Cell Count 136 91 32 113 167 131 168 78 197 182 196 230 180 185 155 52 109 91 62 77 132 157


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.0 35.1 29.2 40.2 40.2 32.6 26.3 – 17.5 21.1 17.2 18.2 – 50.4 46.4 45.2 47.8 47.0 42.3 40.4 41.5
Std Deviation (1e-4) 12.8 12.8 15.0 13.3 14.8 10.6 7.1 – 6.5 7.0 5.2 6.6 – 15.8 14.5 16.4 18.1 18.6 15.5 14.6 15.2
Intensity Change (Log2) – – – 0.46 0.46 0.16 -0.15 – -0.74 -0.47 -0.76 -0.68 – 0.79 0.67 0.63 0.71 0.69 0.53 0.47 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Golgi -1.3 0.6 0 0 – 0 0 0 0 – 0.4 -0.2 2.3 1.4 1.1
Mitochondria 4.2 2.6 4.8 4.8 – 3.1 5.0 7.2 6.0 – 3.1 3.1 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 41.9111 66.6497 58.9415 54.9594 44.146 54.9986 71.46 82.1581 75.8026 51.6877 75.3789 75.61 76.7243 85.385 81.4392 75.4428 59.1356 73.9482
Actin 0.0196 0.0023 0.001 0.0017 0.0368 0.0074 0.0044 0.0016 0.0012 0.0054 0.0008 0.0002 0.0076 0.0008 0.0047 0.0005 0.0202 0.0047
Bud 0.0002 0 0 0 0.0003 0.0002 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0011 0.0001
Bud Neck 0.0002 0 0 0 0.0002 0.0002 0 0 0 0.0002 0 0 0.001 0 0.0002 0 0.0004 0.0001
Bud Periphery 0.0002 0 0.0001 0 0.0005 0.0004 0.0001 0 0 0.0001 0 0 0.0001 0 0.0003 0.0001 0.0024 0.0001
Bud Site 0.0006 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0001 0.0003 0 0 0.0013 0.0002 0.0003 0.0001 0.0019 0.0001
Cell Periphery 0.0006 0.0005 0.0004 0 0.0002 0.0001 0.0002 0.0006 0.0002 0.0001 0.0002 0 0.0003 0.0043 0.0001 0.0002 0.0004 0.0002
Cytoplasm 0.0016 0 0.0001 0 0.0012 0.0023 0.001 0 0 0 0 0 0.0018 0 0.0002 0.0003 0.0016 0.0002
Cytoplasmic Foci 0.0242 0 0.0005 0.0001 0.0537 0.001 0.0027 0 0.0003 0.012 0.0001 0 0.0076 0.0005 0.0081 0.0067 0.0345 0.0142
Eisosomes 0.0009 0.0006 0.0006 0.0006 0.0008 0.0001 0.0014 0.0015 0.0037 0.0003 0.0007 0.0003 0.0024 0.001 0.0023 0.0009 0.001 0.0009
Endoplasmic Reticulum 0.0013 0 0.0002 0 0.0028 0.0056 0.0002 0 0 0 0.0001 0 0.0008 0 0.0003 0.0001 0.0017 0.0004
Endosome 0.0042 0 0.0027 0 0.0181 0.026 0.0035 0.0001 0 0.0064 0.0006 0 0.0037 0.0001 0.0035 0.0003 0.0213 0.0031
Golgi 0.0048 0.0001 0.0005 0 0.0074 0.0025 0.0066 0.0002 0.0002 0.0142 0.0067 0.0001 0.002 0.0008 0.0026 0.0004 0.0064 0.0026
Lipid Particles 0.0388 0.0021 0.0025 0.0002 0.0092 0.0009 0.0091 0.0022 0.0043 0.0891 0.0093 0.001 0.0193 0.0119 0.006 0.0105 0.0264 0.0082
Mitochondria 0.8527 0.984 0.955 0.9936 0.8308 0.9098 0.9443 0.9735 0.9737 0.8371 0.9605 0.9922 0.9212 0.9579 0.9584 0.9525 0.7846 0.9352
None 0.0009 0 0.0002 0 0.0061 0.0005 0.0006 0 0.0001 0.0001 0 0 0.0044 0 0.0014 0.0019 0.0028 0.0006
Nuclear Periphery 0.0012 0 0.0071 0 0.0052 0.03 0.0034 0.0001 0 0.0001 0.0003 0 0.0008 0.0001 0.0011 0.0002 0.008 0.0012
Nucleolus 0.0035 0.0054 0.0016 0.0002 0.013 0.0002 0.0053 0.0004 0.0068 0.0002 0.0001 0 0.0096 0.003 0.0009 0.0023 0.0226 0.0107
Nucleus 0.0006 0.0004 0.0005 0 0.0009 0.0057 0.0025 0.0001 0.0005 0 0.0001 0 0.0007 0.001 0.0002 0.0004 0.0025 0.0006
Peroxisomes 0.0277 0.0017 0.0025 0.0031 0.0043 0.0002 0.004 0.0041 0.0024 0.0325 0.0005 0.0006 0.0033 0.0013 0.0041 0.0017 0.0119 0.0015
Punctate Nuclear 0.01 0 0.0008 0 0.0014 0.0012 0.0043 0 0 0.0004 0 0 0.0002 0 0.0008 0.0004 0.0076 0.0002
Vacuole 0.0013 0.0004 0.0049 0 0.0008 0.0014 0.0009 0.0025 0.0005 0.0004 0.0007 0.0001 0.0007 0.0028 0.0004 0.0011 0.0031 0.0007
Vacuole Periphery 0.0049 0.0023 0.0188 0.0002 0.0058 0.0042 0.0053 0.0129 0.0058 0.001 0.0194 0.0055 0.0111 0.0141 0.0039 0.0195 0.0376 0.0144

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.2473 78.0268 54.9861 38.9127 86.4773 53.768 78.1485 53.2473 50.5199 86.2134
Translational Efficiency 1.4242 1.2188 1.1173 1.2416 1.3396 1.4109 1.1567 1.4082 1.1068 1.2233

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mae1

Mae1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mae1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available