ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0.11 0.05 0.07 – 0.12 0.08 0.09 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.25 0.17 0.15 0.13 0.1 – 0.06 0.06 0.05 0.08 0.07 0.11 0.1 0.11 0.18 0.16 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.36 0.23 0.16 0.12 0.44 0.2 – 0.21 0.08 0 0.05 0.06 0.41 0.33 0.4 0.37 0.45 0.37 0.37 0.35 0.4 0.31 0.25 0.25
Golgi 0.05 0.12 0 0 0.05 0 – 0 0 0 0 0 0.22 0.42 0.3 0.05 0 0 0.31 0.29 0.19 0.43 0.47 0.52
Mitochondria 0.47 0.33 0.66 0.66 0.31 0.7 – 0.64 0.77 0.8 0.82 0.7 0.36 0.26 0.24 0.07 0.11 0.08 0.09 0.14 0.14 0.1 0.1 0.08
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.05 0.05 0.06 – 0.12 0.08 0.05 0 0.11 0.08 0 0 0 0 0 0 0 0.06 0.06 0.05 0
SpindlePole 0.05 0 0.1 0 0.07 0.05 – 0.09 0.07 0.06 0 0.07 0 0.07 0.08 0.1 0.1 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.2 0.08 0 0 0 – 0 0 0.05 0 0 0 0 0.06 0.53 0.37 0.51 0.15 0.15 0.18 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 7 3 6 6 5 – 15 1 8 9 7 7 1 2 5 11 6 10 9 2 5 6 10
Bud 13 1 20 20 11 25 – 65 20 31 22 35 2 1 2 3 9 0 1 0 0 1 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 1 0 0 0 – 8 3 2 6 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 0 – 1 1 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 92 54 49 29 30 38 – 32 16 16 20 28 27 10 18 51 39 26 8 1 1 0 0 3
Endoplasmic Reticulum 6 8 1 6 5 6 – 2 2 1 2 5 12 3 3 8 5 7 3 1 2 2 3 4
Endosome 251 50 47 22 99 74 – 113 20 13 12 24 97 33 64 103 110 58 292 104 126 82 51 72
Golgi 38 26 2 8 11 8 – 9 0 8 8 12 53 42 48 15 11 4 248 84 58 115 98 148
Mitochondria 331 71 188 126 71 260 – 344 194 265 215 269 86 26 38 19 28 13 74 40 43 27 20 21
Nucleus 1 0 0 0 0 0 – 1 0 3 1 1 0 0 0 1 3 1 0 0 0 0 1 0
Nuclear Periphery 0 1 0 2 0 0 – 0 1 1 1 3 1 2 0 0 0 0 1 0 0 0 0 0
Nucleolus 2 1 1 2 1 8 – 24 9 13 7 11 2 1 1 3 4 0 0 0 0 0 0 0
Peroxisomes 27 0 14 10 11 23 – 63 21 17 11 42 19 2 7 7 2 1 20 3 17 15 9 5
SpindlePole 32 6 29 6 15 17 – 46 18 19 11 27 10 7 13 29 25 21 5 2 1 0 1 0
Vac/Vac Membrane 139 42 24 3 9 12 – 15 5 15 11 16 8 2 9 149 92 80 122 44 55 13 11 15
Unique Cell Count 700 215 286 190 226 372 534 251 331 262 382 239 101 161 279 247 157 792 297 314 268 209 285
Labelled Cell Count 943 268 379 240 270 476 738 311 412 336 490 325 130 205 393 339 217 792 297 314 268 209 285


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.9 4.8 5.8 6.2 5.5 – 5.7 5.8 5.9 6.3 6.2 6.6 6.9 6.7 9.3 9.0 8.4 7.2 7.6 7.4
Std Deviation (1e-4) 1.0 1.2 1.1 1.3 1.5 1.2 – 1.1 1.5 1.3 1.3 1.4 1.1 1.1 1.1 1.7 1.7 1.6 1.5 1.5 1.4
Intensity Change (Log2) – – – 0.26 0.35 0.18 – 0.23 0.25 0.29 0.38 0.36 0.45 0.51 0.47 0.95 0.9 0.79 0.56 0.65 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 1.6 0 1.3 1.9 0 0 0 0 0 2.5 0
Bud 1.4 -1.0 -0.1 – 2.3 0.4 1.1 0.6 1.0 -3.5 -2.3 -2.7 -3.6 -1.7 -3.4
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -1.2 -2.6 – -5.1 -3.8 -5.0 -3.3 -3.9 -1.9 -1.7 -1.7 0.4 -0.4 -0.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 3.4 0 0 0 0 0
Endosome -1.5 6.8 1.1 – 1.6 -3.0 -5.2 -4.5 -4.2 6.2 3.5 5.5 5.5 7.1 4.9
Golgi 0 3.0 0 – 0 0 0 0 2.2 8.0 11.1 9.4 3.3 2.8 0
Mitochondria 0.1 -7.7 1.1 – -0.4 2.9 4.0 4.3 1.3 -6.8 -6.9 -8.6 -14.5 -12.8 -11.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 3.3 0 3.0 0 2.4 0 0 0 0 0 0
Peroxisomes 0.2 0 0.7 – 3.2 1.6 0.1 -0.4 2.8 1.4 0 -0.3 -1.5 -2.8 -2.4
SpindlePole -2.9 -1.4 -2.8 – -0.7 -1.2 -2.0 -2.7 -1.4 -2.6 -1.0 -0.7 0.1 0 1.0
Vacuole -3.1 -2.0 -2.9 – -3.6 -3.3 -2.0 -2.0 -2.3 -2.4 -2.2 -1.1 11.6 8.1 10.1