Standard name | |
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Human Ortholog | |
Description | Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 4 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 5 | 8 | 7 | 10 | 16 | 12 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 3 | 4 | 12 | 14 | 16 | 21 | 15 | 16 | 13 | 8 | 11 | 27 | 41 | 22 | 12 | 3 | 0 | 0 | 0 | 0 | 2 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Endosome | 0 | 1 | 0 | 0 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | 3 | 6 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 47 | 7 | 25 | 15 | 19 | 119 | 149 | 174 | 194 | 291 | 183 | 198 | 6 | 1 | 5 | 16 | 12 | 1 | 1 | 0 | 2 | 1 | 1 | 3 |
Nucleus | 116 | 178 | 168 | 135 | 199 | 231 | 281 | 328 | 201 | 284 | 193 | 164 | 162 | 244 | 203 | 177 | 132 | 53 | 112 | 175 | 163 | 57 | 113 | 105 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 4 | 2 | 3 | 3 | 4 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 14 | 11 | 12 | 8 | 15 | 27 | 30 | 20 | 32 | 59 | 24 | 28 | 5 | 5 | 12 | 4 | 1 | 1 | 3 | 5 | 13 | 1 | 24 | 22 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 2 | 3 | 3 | 2 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 3 | 4 |
Vac/Vac Membrane | 1 | 5 | 5 | 2 | 5 | 6 | 12 | 7 | 9 | 3 | 2 | 9 | 2 | 7 | 4 | 8 | 5 | 1 | 7 | 5 | 9 | 1 | 9 | 23 |
Unique Cell Count | 125 | 187 | 188 | 148 | 233 | 292 | 392 | 444 | 324 | 449 | 298 | 274 | 176 | 281 | 257 | 210 | 153 | 58 | 131 | 191 | 198 | 69 | 166 | 182 |
Labelled Cell Count | 182 | 205 | 213 | 168 | 257 | 405 | 505 | 568 | 470 | 680 | 434 | 432 | 186 | 288 | 272 | 236 | 170 | 60 | 131 | 191 | 198 | 69 | 166 | 182 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.9 | 5.4 | 5.3 | 5.5 | 4.3 | 4.0 | 4.2 | 3.9 | 3.5 | 3.7 | 3.5 | 6.1 | 7.5 | 5.7 | 6.3 | 7.4 | 8.2 | 6.9 | 6.3 | 6.7 |
Std Deviation (1e-4) | 0.9 | 0.9 | 1.0 | 1.5 | 2.1 | 1.1 | 1.3 | 1.5 | 1.2 | 0.9 | 1.3 | 0.8 | 1.7 | 18.0 | 1.5 | 1.8 | 2.0 | 1.9 | 3.1 | 2.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.01 | -0.34 | -0.43 | -0.36 | -0.48 | -0.62 | -0.57 | -0.63 | 0.17 | 0.47 | 0.06 | 0.22 | 0.45 | 0.59 | 0.34 | 0.21 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.0 | 1.9 | 1.6 | 1.9 | 1.8 | 1.3 | 1.7 | 0 | 2.3 | 3.5 | 5.0 | 3.6 | 2.8 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.9 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.4 | -6.0 | -4.7 | -1.9 | -1.6 | -2.5 |
Nucleus | 0.6 | -1.2 | -2.9 | -4.8 | -4.3 | -6.6 | -6.6 | -6.0 | -6.9 | 0.9 | -0.8 | -2.9 | -1.5 | -0.9 | 0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.6 | -2.6 | -0.8 | -2.3 | -2.7 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0.3 | 0 | 0.1 | 0 | 0 | 0.4 | 0 | 0 | 0 | 0.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7679 | 1.4092 | 0.9173 | 0.851 | 0.5032 | 1.148 | 2.1294 | 2.5869 | 2.3538 | 1.9251 | 1.6853 | 2.2337 | 3.0553 | 3.3247 | 3.0534 | 3.1451 | 2.8528 | 3.0225 |
Actin | 0.0072 | 0 | 0.003 | 0 | 0.0082 | 0 | 0.0259 | 0 | 0.0137 | 0.0284 | 0.0116 | 0.0003 | 0.0261 | 0 | 0.0007 | 0 | 0.001 | 0.0001 |
Bud | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0017 | 0.0011 | 0.0046 | 0.0001 | 0.001 | 0.0013 | 0.0004 | 0.0007 | 0.0022 | 0.0001 | 0.0007 | 0.0002 | 0.0021 | 0.0008 |
Bud Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0023 | 0 | 0.001 | 0 | 0.0006 | 0.001 | 0.0001 | 0 | 0.0006 | 0 | 0 | 0 | 0.0013 | 0 |
Bud Site | 0.0002 | 0 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0095 | 0 | 0.0034 | 0.0009 | 0.0004 | 0.0001 | 0.001 | 0 | 0.0002 | 0 | 0.0009 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 |
Cytoplasm | 0.0004 | 0.0001 | 0.0008 | 0.0009 | 0.0005 | 0.0002 | 0.0125 | 0.0042 | 0.0006 | 0.0028 | 0.0014 | 0.0009 | 0.001 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0042 | 0 | 0.0037 | 0 | 0.0015 | 0 | 0.0118 | 0.0001 | 0.008 | 0.0127 | 0.0041 | 0.0002 | 0.003 | 0 | 0.0027 | 0 | 0.0003 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.001 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0031 | 0.0002 | 0.0007 | 0.0026 | 0.0001 | 0.0003 | 0.0013 | 0 | 0.0002 | 0 | 0.0013 | 0.0001 |
Endosome | 0.0039 | 0 | 0.0027 | 0.0001 | 0.0057 | 0 | 0.0138 | 0.0003 | 0.0051 | 0.0056 | 0.0033 | 0.0022 | 0.0074 | 0 | 0.0066 | 0 | 0.0033 | 0 |
Golgi | 0.0029 | 0 | 0.0041 | 0 | 0.0032 | 0 | 0.0058 | 0 | 0.0037 | 0.0029 | 0.0164 | 0.0003 | 0.0056 | 0 | 0.0013 | 0 | 0.0009 | 0 |
Lipid Particles | 0.0226 | 0 | 0.0071 | 0 | 0.0118 | 0 | 0.0152 | 0 | 0.0186 | 0.0072 | 0.0015 | 0.0018 | 0.0061 | 0 | 0.0041 | 0 | 0.0022 | 0 |
Mitochondria | 0.0098 | 0.0001 | 0.0088 | 0.0002 | 0.0185 | 0.0001 | 0.0076 | 0.0001 | 0.005 | 0.0045 | 0.0021 | 0.0005 | 0.0045 | 0.0001 | 0.0003 | 0.0001 | 0.0055 | 0.0002 |
None | 0.0006 | 0 | 0.006 | 0.0007 | 0.001 | 0.0002 | 0.0149 | 0.0021 | 0.0011 | 0.0072 | 0.0311 | 0.0026 | 0.0057 | 0.0001 | 0.0059 | 0.0006 | 0.0023 | 0 |
Nuclear Periphery | 0.0043 | 0.0005 | 0.0018 | 0.003 | 0.0164 | 0.0004 | 0.0125 | 0.0035 | 0.0056 | 0.0095 | 0.0035 | 0.0073 | 0.0095 | 0.0014 | 0.0011 | 0.001 | 0.0039 | 0.0011 |
Nucleolus | 0.0167 | 0.0108 | 0.0089 | 0.0138 | 0.0485 | 0.034 | 0.0168 | 0.0163 | 0.0193 | 0.0046 | 0.028 | 0.0341 | 0.0227 | 0.0118 | 0.0089 | 0.0086 | 0.0426 | 0.0397 |
Nucleus | 0.9195 | 0.9875 | 0.9199 | 0.9779 | 0.8647 | 0.9618 | 0.8028 | 0.9509 | 0.8739 | 0.8858 | 0.8839 | 0.9436 | 0.8926 | 0.9858 | 0.9572 | 0.9879 | 0.9086 | 0.9468 |
Peroxisomes | 0.0044 | 0 | 0.014 | 0 | 0.0019 | 0 | 0.0117 | 0 | 0.0141 | 0.0175 | 0.0022 | 0.0001 | 0.0014 | 0 | 0.0005 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.0014 | 0.0008 | 0.0171 | 0.0029 | 0.0055 | 0.002 | 0.0249 | 0.0221 | 0.0225 | 0.0019 | 0.0097 | 0.0044 | 0.0067 | 0.0005 | 0.0081 | 0.0014 | 0.0183 | 0.0109 |
Vacuole | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0021 | 0.0001 | 0.0027 | 0.0001 | 0.0015 | 0.0015 | 0.0001 | 0.0003 | 0.0009 | 0 | 0.0003 | 0 | 0.0032 | 0.0001 |
Vacuole Periphery | 0.0009 | 0 | 0.0002 | 0 | 0.0033 | 0 | 0.0011 | 0 | 0.0009 | 0.0009 | 0.0001 | 0.0003 | 0.0009 | 0 | 0.0001 | 0 | 0.0007 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.8421 | 11.8315 | 7.8553 | 14.926 | 15.3538 | 14.6204 | 11.3332 | 10.1989 | 10.6475 | 15.8507 |
Translational Efficiency | 1.7276 | 1.6631 | 2.148 | 1.3833 | 1.3268 | 1.184 | 1.553 | 1.3754 | 1.4981 | 1.1631 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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