Standard name
Human Ortholog
Description Protein that promotes the transport and maturation of Pma1 with Exp1; cleaved specifically by Kex2p; localizes to the mid-Golgi and COPI vesicles
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.08 0.2 0.2 0.31 0.32 0.34 0.35 0.4 0.47 0.5 0.11 0.15 0.25 0.09 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.32 0.33 0.23 0.1 0.08 0.12 0.06 0.06 0.05 0 0 0
Mitochondria 0.1 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.93 0.86 0.89 0.89 0.87 0.76 0.75 0.73 0.72 0.64 0.56 0.58 0.73 0.73 0.67 0.84 0.87 0.88 0.84 0.85 0.85 0.9 0.89 0.9
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 2 1 0 0 1 0 4 9 4 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 1 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 25 25 39 49 92 128 153 107 151 139 132 20 61 85 23 23 18 0 1 0 0 1 2
Endoplasmic Reticulum 0 3 1 0 3 7 5 4 4 6 8 8 4 7 5 10 12 11 1 0 3 0 0 0
Endosome 0 0 1 2 5 7 3 6 3 2 3 2 10 25 25 1 0 3 7 2 11 3 7 4
Golgi 1 9 13 2 3 0 2 2 2 2 3 2 61 131 78 24 24 31 14 13 14 3 4 4
Mitochondria 23 7 5 5 11 9 16 20 8 18 20 18 3 2 6 5 2 6 1 2 5 1 1 2
Nucleus 9 13 11 3 0 4 10 10 8 6 4 2 0 0 1 21 25 16 5 6 2 5 7 4
Nuclear Periphery 0 0 0 1 4 2 2 3 1 0 0 3 0 1 3 1 0 0 4 2 5 2 2 6
Nucleolus 0 1 3 2 1 3 3 5 1 3 4 2 1 1 6 4 1 1 0 0 0 0 3 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 206 191 278 175 218 226 297 328 219 243 166 153 138 292 230 208 260 226 200 195 266 203 259 257
Unique Cell Count 222 223 313 196 250 299 394 451 304 380 297 263 190 398 342 247 298 257 238 230 314 226 290 286
Labelled Cell Count 245 249 337 229 295 351 468 532 356 431 348 325 242 532 444 298 347 312 238 230 314 226 290 286


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 23.5 17.6 17.0 17.3 15.6 14.8 14.0 14.0 12.7 12.1 11.5 16.8 14.8 12.3 26.6 27.0 27.3 19.4 18.5 19.1
Std Deviation (1e-4) 4.6 8.2 5.2 4.7 4.8 5.1 5.3 5.0 4.6 4.2 3.9 3.5 4.7 3.1 2.5 10.8 9.6 9.9 6.6 6.1 5.7
Intensity Change (Log2) – – – -0.04 -0.02 -0.17 -0.25 -0.32 -0.33 -0.46 -0.53 -0.62 -0.06 -0.24 -0.52 0.6 0.62 0.64 0.15 0.08 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.9 4.1 7.2 7.9 8.3 8.2 9.6 10.8 11.3 1.0 3.0 5.8 0.6 -0.1 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.3
Endosome 0 0 0 0 0 0 0 0 0 0 4.2 4.6 0 0 0
Golgi -2.0 -2.1 -3.6 -3.3 -3.6 -2.8 -3.3 -2.4 -2.5 8.6 9.5 6.9 2.6 2.0 3.5
Mitochondria 0 2.0 1.2 1.9 2.2 0.9 2.3 3.2 3.2 0 0 0.2 0 0 0
Nucleus -1.3 0 -1.7 -0.8 -1.1 -0.6 -1.6 -1.7 -2.2 0 0 -3.1 2.5 2.6 1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 -0.6 -4.3 -4.6 -5.4 -5.3 -7.5 -9.1 -8.4 -4.7 -5.1 -6.6 -1.6 -0.6 -0.3