Standard name
Human Ortholog
Description Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0.06 0 0 0.08 0.11 0.07 0.07 0.13 0.21 0.21 0.46 0.44 0.42 0 0 0 0 0 0
Cytoplasm 0.17 0.11 0.14 0.15 0.11 0.1 0.09 0.19 0.19 0.2 0.23 0.07 0.08 0.12 0.44 0.53 0.39 0.05 0 0 0.06 0.05 0.06
Endoplasmic Reticulum 0.85 0.89 0.83 0.84 0.87 0.86 0.87 0.74 0.73 0.73 0.68 0.88 0.8 0.74 0 0 0 0.88 0.92 0.91 0.89 0.86 0.84
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 4 5 4 1 3 4 5 4 5 6 7 4 0 1 0 5 4 2 0 3 6
Bud 5 2 8 5 2 2 3 3 3 3 6 0 2 3 0 0 1 5 0 0 0 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 14 8 11 17 11 19 20 32 17 17 29 46 53 36 19 45 2 1 2 0 2 3
Cytoplasm 61 47 33 30 33 25 38 51 57 48 54 16 19 29 35 23 41 23 16 9 9 11 17
Endoplasmic Reticulum 313 374 191 172 264 217 377 193 214 173 160 203 179 184 2 1 3 383 432 236 140 199 258
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 1 1 0 3 0 2 1 0 1 0 5 2 2 4 2 8 1 0 0 0 4 2
Mitochondria 0 0 1 0 0 0 0 0 0 0 1 0 0 1 4 2 12 1 1 2 1 1 2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 2
Nucleolus 0 0 0 0 0 1 6 3 3 4 1 0 0 0 0 0 2 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 3 1 2 0 2 1 1 3 0 2 2 1 1 5 1 0 0 4 1 2 1 1 3
Unique Cell Count 368 418 230 205 304 252 431 262 295 237 235 231 224 247 79 43 106 434 468 261 158 232 308
Labelled Cell Count 390 439 248 223 325 258 449 278 314 252 246 260 256 281 83 48 114 434 468 261 158 232 308


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.0 109.3 39.0 60.8 77.8 85.5 97.7 98.9 101.5 102.8 100.3 58.4 54.3 48.8 145.5 138.3 134.1 78.0 87.4 84.8
Std Deviation (1e-4) 24.0 26.9 12.0 18.2 30.6 22.4 27.7 29.6 30.2 31.6 31.2 19.8 19.8 17.0 59.0 50.8 57.6 27.3 34.0 32.7
Intensity Change (Log2) 0.64 1.0 1.13 1.33 1.34 1.38 1.4 1.36 0.58 0.48 0.33 1.9 1.83 1.78 1.0 1.17 1.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 1.0 0 0 0 0
Bud -0.6 0 0 0 -1.7 0 -1.6 -0.6 0 0 -1.6 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 1.1 0.5 0.6 2.0 3.2 1.8 1.8 3.6 5.6 5.9 9.2 0 9.1
Cytoplasm 0.1 -1.2 -1.5 -2.2 1.5 1.5 1.7 2.4 -2.6 -2.0 -0.8 5.5 5.8 5.0
Endoplasmic Reticulum 0.2 1.2 0.9 1.6 -2.5 -2.8 -2.6 -3.7 1.5 -0.9 -2.3 -12.7 -10.6 -13.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 100.2785 128.4584 121.8639 134.6544 93.5093 135.5768 144.8634 150.3519 139.2966 133.3342 129.1385 181.5782 120.9868 115.6611 105.7803 143.567 104.9985 132.0833
Actin 0.0269 0.0002 0.0044 0.0001 0.004 0.0003 0.0054 0.0003 0.0045 0.0421 0.0073 0.0017 0.0011 0.0007 0.0097 0.0001 0.0017 0.0003
Bud 0.0005 0 0.0002 0 0.0016 0 0.0001 0 0.0001 0.0006 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0001 0
Bud Neck 0.0004 0.0006 0.0005 0.0015 0.0011 0.0034 0.0003 0.0003 0.0005 0.0007 0.0007 0.0146 0.0004 0.0004 0.0005 0.001 0.0009 0.0008
Bud Periphery 0.0008 0.0001 0.0002 0.0001 0.0023 0.0002 0.0002 0.0002 0.0002 0.0006 0.0008 0.0009 0.0003 0.0002 0.0003 0.0002 0.0002 0
Bud Site 0.0021 0.0001 0.0034 0 0.0172 0 0.0003 0.0002 0.0006 0.0029 0.0002 0.0001 0.0003 0.0004 0.0004 0 0.0002 0
Cell Periphery 0.0269 0.0668 0.0609 0.0504 0.052 0.031 0.0334 0.0877 0.0559 0.0595 0.0343 0.0545 0.0418 0.0578 0.0731 0.1495 0.0517 0.0161
Cytoplasm 0.0154 0.0275 0.0109 0.0143 0.005 0.0125 0.0176 0.0199 0.0237 0.0074 0.0029 0.0329 0.0396 0.0438 0.0351 0.0568 0.0333 0.0022
Cytoplasmic Foci 0.0134 0.0001 0.0108 0.0001 0.015 0 0.0112 0.0001 0.0139 0.0239 0.0008 0.0002 0.0064 0.0006 0.0041 0.0002 0.0044 0
Eisosomes 0.0036 0.0011 0.0093 0.0011 0.0028 0.0048 0.0071 0.0099 0.0017 0.0073 0.0005 0.0014 0.0012 0.0087 0.0013 0.0115 0.0093 0.0003
Endoplasmic Reticulum 0.6477 0.8035 0.7769 0.7781 0.6091 0.811 0.5644 0.757 0.6892 0.553 0.6809 0.6558 0.6475 0.7919 0.7475 0.5402 0.6775 0.837
Endosome 0.0067 0.0002 0.0006 0.0002 0.0102 0.0002 0.0052 0.0002 0.0059 0.0018 0.0384 0.0007 0.0012 0.0006 0.0038 0.0003 0.0013 0.0002
Golgi 0.0034 0.0003 0.0007 0.0004 0.0056 0.0007 0.0013 0.001 0.0021 0.0021 0.0099 0.0008 0.0006 0.001 0.002 0.0009 0.0043 0.0008
Lipid Particles 0.0194 0.0117 0.0079 0.017 0.0162 0.0047 0.0056 0.0099 0.0099 0.0139 0.0092 0.0036 0.0131 0.0155 0.0091 0.0073 0.0127 0.0055
Mitochondria 0.0157 0.0001 0.0075 0 0.0193 0.0001 0.0032 0.0002 0.0056 0.0104 0.008 0.0004 0.0017 0.0024 0.0025 0.0001 0.0082 0
None 0.0129 0.0003 0.0067 0.0001 0.0135 0.0002 0.0069 0.0002 0.0169 0.0348 0.0012 0.0006 0.0044 0.0006 0.0058 0.0005 0.0077 0.0001
Nuclear Periphery 0.1571 0.07 0.08 0.1225 0.1396 0.1163 0.2969 0.0924 0.1328 0.2095 0.1688 0.1738 0.1851 0.0546 0.08 0.2005 0.116 0.1296
Nucleolus 0.0111 0.0001 0.0022 0 0.0413 0.0002 0.0041 0.0001 0.0098 0.0047 0.0002 0.0013 0.012 0.0006 0.0017 0.0003 0.0447 0
Nucleus 0.008 0.0011 0.0012 0.001 0.0103 0.0017 0.0134 0.0011 0.0049 0.0062 0.002 0.0185 0.0198 0.0019 0.0029 0.0024 0.0059 0.002
Peroxisomes 0.0026 0 0.0007 0 0.0034 0 0.0005 0 0.0005 0.0008 0.0001 0 0.0002 0.0001 0.0005 0 0.0004 0
Punctate Nuclear 0.0043 0.0001 0.0024 0 0.0025 0.0001 0.0041 0 0.0033 0.0016 0.0004 0.0002 0.0024 0.0002 0.0023 0.0002 0.0015 0.0001
Vacuole 0.0126 0.015 0.011 0.0106 0.0152 0.0104 0.014 0.0183 0.0129 0.0101 0.0163 0.0295 0.0171 0.0165 0.0144 0.0257 0.0104 0.0035
Vacuole Periphery 0.0084 0.0011 0.0016 0.0023 0.0129 0.0023 0.0049 0.0011 0.0054 0.0061 0.0166 0.0083 0.0037 0.0016 0.0029 0.0023 0.0075 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2974.0507 3337.4162 2932.9816 2201.1379 3220.1591 1928.9006 2292.2365 2042.2994 1661.7495 1744.105
Translational Efficiency 1.0775 1.2237 1.1392 1.6477 1.1635 1.4718 1.4981 1.6589 1.8108 1.69

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
54 1493 1180 1308 1745 1565 30 995 1799 3058 1210 2303

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3130.74 5525.05 15042.72 17359.57 3365.21 5693.08 12348.38 16579.51 3358.17 5611.04 14975.92 17022.55
Standard Deviation 1061.78 1747.68 3679.19 5164.88 976.98 1712.33 2667.66 5213.47 980.45 1731.72 3681.42 5200.30
Intensity Change Log 2 0.819484 2.264490 2.471156 0.758513 1.875553 2.300633 0.788220 2.076095 2.385335

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000497 0.000342 0.000436 0.000772 0.000833 0.001470 0.000415 0.001065 0.000823 0.000919 0.000436 0.000899
Bud Neck 0.002508 0.001610 0.012310 0.017808 0.001159 0.002787 0.012953 0.022119 0.001200 0.002212 0.012326 0.019670
Bud Site 0.002069 0.001726 0.001895 0.016933 0.001757 0.002142 0.005522 0.021870 0.001767 0.001939 0.001985 0.019066
Cell Periphery 0.043637 0.013343 0.063555 0.057111 0.010227 0.013105 0.173109* 0.074380 0.011230 0.013221 0.066271 0.064572
Cytoplasm 0.001763 0.001219 0.034379 0.128467* 0.001551 0.005012 0.070330 0.097809 0.001557 0.003161 0.035270 0.115221*
Cytoplasmic Foci 0.001220 0.000265 0.000792 0.005241 0.000467 0.000965 0.003515 0.008315 0.000489 0.000623 0.000860 0.006569
Eisosomes 0.002897 0.000841 0.000399 0.000090 0.000487 0.000671 0.000246 0.000158 0.000560 0.000754 0.000396 0.000119
Endoplasmic Reticulum 0.777065* 0.938378* 0.603524* 0.274006* 0.930019* 0.885292* 0.044673 0.273081* 0.925428* 0.911210* 0.589668* 0.273606*
Endosome 0.012325 0.004528 0.006626 0.011420 0.004802 0.008294 0.002030 0.013737 0.005027 0.006456 0.006512 0.012421
Golgi 0.025115 0.005141 0.011062 0.007027 0.008397 0.007955 0.003995 0.009893 0.008899 0.006581 0.010887 0.008265
Lipid Particles 0.037551 0.006711 0.025947 0.115967* 0.009500 0.016966 0.178867* 0.147712* 0.010342 0.011959 0.029739 0.129683*
Mitochondria 0.002444 0.000772 0.001669 0.001408 0.001481 0.001940 0.000214 0.001634 0.001510 0.001370 0.001633 0.001506
Mitotic Spindle 0.000203 0.000119 0.000334 0.016279 0.000089 0.001070 0.000871 0.013959 0.000093 0.000606 0.000347 0.015276
None 0.000705 0.001120 0.001886 0.007514 0.001949 0.001714 0.000779 0.006054 0.001912 0.001424 0.001858 0.006883
Nuclear Periphery 0.004274 0.005383 0.062228 0.025849 0.004666 0.010726 0.009526 0.020962 0.004655 0.008117 0.060922 0.023738
Nuclear Periphery Foci 0.014891 0.002914 0.042739 0.117437 0.004415 0.014741 0.015287 0.092391 0.004729 0.008967 0.042058 0.106616
Nucleolus 0.000412 0.000026 0.000100 0.000256 0.000210 0.000122 0.000063 0.000197 0.000216 0.000075 0.000099 0.000230
Nucleus 0.008248 0.000492 0.005816 0.025138 0.000736 0.001526 0.034848 0.020715 0.000961 0.001021 0.006536 0.023227
Peroxisomes 0.000128 0.000009 0.000011 0.000087 0.000019 0.000029 0.000033 0.000141 0.000022 0.000019 0.000012 0.000110
Vacuole 0.055320 0.012148 0.109069 0.164283 0.010839 0.019012 0.439418* 0.166569 0.012174 0.015661 0.117259 0.165270
Vacuole Periphery 0.006726 0.002914 0.015221 0.006905 0.006396 0.004462 0.003304 0.007240 0.006405 0.003706 0.014926 0.007050

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.68 -1.18 -3.22 -4.45 -2.38 -1.47 0.67 -1.49 0.42 -2.08 -0.42 0.36 -1.80 -1.36 -2.89
Bud Neck 0.95 -9.46 -15.71 -16.99 -6.78 -4.34 -2.87 -14.43 -13.36 -2.86 -3.52 -11.88 -22.56 -21.32 -8.47
Bud Site 0.24 -0.71 -10.64 -10.88 -10.77 -1.20 -1.47 -10.13 -9.87 -4.29 -0.66 -1.59 -14.78 -14.68 -14.26
Cell Periphery 1.83 -1.33 -0.27 -11.89 4.41 -2.13 -9.17 -15.13 -13.98 6.09 -1.60 -17.53 -18.48 -18.26 3.66
Cytoplasm -0.01 -10.56 -23.06 -23.46 -15.56 -3.58 -2.84 -18.75 -17.81 -1.73 -2.75 -11.50 -29.82 -29.24 -17.24
Cytoplasmic Foci 0.90 -0.03 -5.61 -8.12 -7.19 -2.03 -1.63 -6.82 -6.39 -1.70 -1.15 -2.94 -10.34 -10.03 -9.20
Eisosomes 1.17 1.43 1.61 5.54 6.20 -1.05 1.80 3.97 3.57 2.04 -1.35 1.54 4.56 6.37 5.82
Endoplasmic Reticulum -3.76 4.75 13.34 80.21* 27.22 6.91 35.89* 68.75* 59.01* -6.23 3.15 31.74 95.20 96.72* 28.33
Endosome 3.00 2.08 0.62 -12.90 -7.87 -7.04 5.70 -11.39 -5.09 -13.60 -4.28 -5.19 -14.83 -10.83 -10.86
Golgi 1.91 1.32 1.83 -1.69 7.94 0.32 2.47 0.30 -0.01 -2.26 2.25 -1.97 2.05 -0.39 5.95
Lipid Particles 2.63 0.77 -6.40 -21.35 -16.22 -3.51 -4.07 -20.76 -19.03 0.49 -1.22 -8.03 -28.35 -28.08 -21.16
Mitochondria 0.70 0.09 0.35 -2.71 1.89 -0.96 4.15 -1.06 0.28 -5.87 0.18 -1.53 -0.60 -0.80 1.32
Mitotic Spindle 0.64 -1.48 -8.19 -8.22 -8.13 -1.56 -1.68 -5.99 -5.43 -5.44 -1.60 -7.09 -10.17 -9.72 -10.02
None -0.73 -1.98 -5.01 -5.10 -4.24 0.22 1.51 -3.01 -3.31 -4.16 0.68 -0.09 -4.50 -5.92 -5.16
Nuclear Periphery -1.09 -20.55 -11.91 -13.16 13.27 -6.90 -1.88 -11.23 -5.70 -3.61 -6.14 -21.35 -17.37 -13.05 14.32
Nuclear Periphery Foci 1.99 -4.92 -12.98 -22.34* -11.99 -6.58 -1.78 -17.10 -14.36 -8.01 -4.44 -14.98 -27.58 -25.90 -13.21
Nucleolus 0.96 0.71 0.25 -5.94 -3.76 0.83 1.40 -0.94 -2.93 -3.69 1.63 1.17 -0.96 -5.93 -5.25
Nucleus 0.91 -0.14 -4.93 -14.56 -11.43 -1.94 -2.74 -12.23 -11.74 0.17 -0.78 -6.69 -18.77 -18.60 -13.26
Peroxisomes 1.11 1.08 0.40 -4.19 -4.05 -0.99 -1.16 -2.05 -1.76 -1.67 0.25 2.39 -3.24 -3.26 -3.71
Vacuole 2.25 -3.00 -5.92 -30.18 -8.60 -5.33 -8.94 -28.64 -26.64 5.13 -2.86 -23.19 -40.74 -40.05 -9.01
Vacuole Periphery 1.49 -3.19 0.43 -3.47 6.96 2.14 2.18 0.44 -1.22 -1.64 3.50 -6.08 0.84 -3.12 6.84
Standard name
Human Ortholog
Description Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Cwp2

Cwp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available