Standard name | |
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Human Ortholog | |
Description | Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 5 | 1 | 4 | 1 | 4 | 8 | 1 | 5 | 0 | 3 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 162 | 286 | 177 | 113 | 151 | 52 | 129 | 148 | 39 | 54 | 53 | 77 | 327 | 430 | 405 | 44 | 161 | 150 | 151 | 274 | 172 | 317 | 345 | 420 |
Endoplasmic Reticulum | 2 | 3 | 0 | 3 | 3 | 4 | 16 | 15 | 8 | 7 | 1 | 7 | 1 | 2 | 2 | 2 | 3 | 4 | 0 | 0 | 1 | 0 | 5 | 2 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 2 | 2 | 14 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 0 | 4 | 3 | 1 | 6 | 12 | 5 | 3 | 4 | 5 | 0 | 2 | 4 | 0 | 6 | 2 | 0 | 1 | 0 | 1 | 5 | 10 |
Unique Cell Count | 165 | 291 | 177 | 121 | 157 | 57 | 153 | 186 | 52 | 67 | 63 | 95 | 330 | 432 | 409 | 45 | 166 | 153 | 157 | 286 | 181 | 326 | 372 | 474 |
Labelled Cell Count | 168 | 294 | 182 | 122 | 161 | 61 | 157 | 190 | 53 | 72 | 65 | 98 | 330 | 437 | 411 | 46 | 173 | 160 | 157 | 286 | 181 | 326 | 372 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 57.3 | 59.0 | 54.9 | 49.7 | 56.8 | 35.3 | 49.0 | 45.6 | 40.7 | 39.7 | 33.1 | 37.2 | 56.9 | 67.0 | 69.8 | 60.8 | 66.6 | 69.8 | 44.9 | 50.6 | 55.6 |
Std Deviation (1e-4) | 14.7 | 13.3 | 9.8 | 12.2 | 14.2 | 13.8 | 15.8 | 13.1 | 12.0 | 15.5 | 14.6 | 12.9 | 12.6 | 17.8 | 19.4 | 29.8 | 28.0 | 25.2 | 11.1 | 14.2 | 16.8 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.05 | -0.64 | -0.16 | -0.27 | -0.43 | -0.47 | -0.73 | -0.56 | 0.05 | 0.29 | 0.35 | 0.15 | 0.28 | 0.35 | -0.29 | -0.12 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.5 | -2.6 | -4.0 | -5.5 | -6.4 | -6.8 | -6.0 | -5.4 | -6.0 | -1.3 | -0.9 | -1.3 | -2.0 | -2.3 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 4.4 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 40.6082 | 66.1 | 55.4646 | 49.5094 | 49.6573 | 53.8977 | 50.064 | 70.4631 | 62.1676 | 60.0146 | 49.6612 | 63.1005 | 66.3155 | 81.0152 | 73.8168 | 70.6415 | 59.9685 | 73.1771 |
Actin | 0.0207 | 0.0059 | 0.0134 | 0.0144 | 0.0013 | 0.0008 | 0.0173 | 0.0001 | 0.0052 | 0.0007 | 0.0008 | 0.0002 | 0.0071 | 0.0001 | 0.0003 | 0.0001 | 0.0213 | 0.0002 |
Bud | 0.0029 | 0.0001 | 0.0008 | 0.0017 | 0.0006 | 0.0002 | 0.0012 | 0 | 0.0005 | 0.0001 | 0.0016 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0035 | 0.0007 | 0.0003 | 0.0019 | 0.0004 | 0.0009 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0008 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0003 |
Bud Periphery | 0.0018 | 0 | 0.0006 | 0.0018 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0005 | 0 | 0 | 0.0002 | 0.0005 | 0 |
Bud Site | 0.0149 | 0.0002 | 0.0007 | 0.0087 | 0.0014 | 0 | 0.0073 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 |
Cell Periphery | 0.0005 | 0 | 0.0003 | 0.0005 | 0.0008 | 0.0001 | 0.0007 | 0.002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 |
Cytoplasm | 0.7741 | 0.9816 | 0.9205 | 0.8777 | 0.8607 | 0.9622 | 0.8183 | 0.9756 | 0.9194 | 0.9308 | 0.8324 | 0.9136 | 0.8795 | 0.9901 | 0.9591 | 0.9415 | 0.8203 | 0.9551 |
Cytoplasmic Foci | 0.0211 | 0.0002 | 0.0066 | 0.0035 | 0.0122 | 0.0024 | 0.0138 | 0.0001 | 0.011 | 0.006 | 0.0174 | 0.0034 | 0.0169 | 0.0001 | 0.0028 | 0.0005 | 0.0062 | 0.0002 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.0002 | 0.0006 | 0.0013 | 0.0018 | 0.0016 | 0.0042 | 0.0003 | 0.0007 | 0.0004 | 0.0037 | 0.0006 | 0.0032 | 0.0001 | 0.0005 | 0.0002 | 0.0085 | 0.0002 |
Endosome | 0.0338 | 0.0007 | 0.0033 | 0.0068 | 0.0265 | 0.0007 | 0.007 | 0 | 0.0034 | 0.0004 | 0.0265 | 0.0031 | 0.0048 | 0 | 0.0004 | 0.001 | 0.0263 | 0.0001 |
Golgi | 0.0056 | 0.0007 | 0.0051 | 0.0023 | 0.0093 | 0.0001 | 0.002 | 0 | 0.0012 | 0.0003 | 0.0049 | 0.0043 | 0.0013 | 0 | 0.0001 | 0 | 0.0109 | 0 |
Lipid Particles | 0.0083 | 0.0001 | 0.0025 | 0.0009 | 0.0115 | 0.0002 | 0.0078 | 0.0002 | 0.0058 | 0.0065 | 0.0091 | 0.0005 | 0.0092 | 0 | 0.0008 | 0.0003 | 0.006 | 0 |
Mitochondria | 0.0068 | 0.0002 | 0.0164 | 0.0047 | 0.01 | 0.0003 | 0.0066 | 0 | 0.0039 | 0.0046 | 0.0057 | 0.0006 | 0.0081 | 0 | 0.0064 | 0.0006 | 0.0074 | 0.0001 |
None | 0.0088 | 0.0001 | 0.0033 | 0.0008 | 0.009 | 0.0011 | 0.0042 | 0.0001 | 0.0032 | 0.0034 | 0.0162 | 0.0003 | 0.0085 | 0.0001 | 0.002 | 0.0006 | 0.0044 | 0.0001 |
Nuclear Periphery | 0.0171 | 0.001 | 0.0034 | 0.0073 | 0.0055 | 0.0035 | 0.0145 | 0.0019 | 0.0037 | 0.0027 | 0.0119 | 0.0056 | 0.0062 | 0.0007 | 0.0023 | 0.003 | 0.0206 | 0.0049 |
Nucleolus | 0.0017 | 0 | 0.0023 | 0.0006 | 0.0104 | 0 | 0.0023 | 0.0001 | 0.001 | 0.0009 | 0.007 | 0.0008 | 0.0052 | 0 | 0.0005 | 0.0015 | 0.003 | 0 |
Nucleus | 0.0276 | 0.0078 | 0.014 | 0.0526 | 0.0189 | 0.0229 | 0.0703 | 0.0183 | 0.0303 | 0.0402 | 0.0396 | 0.0587 | 0.0269 | 0.0088 | 0.0211 | 0.0316 | 0.0306 | 0.0378 |
Peroxisomes | 0.0122 | 0.0001 | 0.0012 | 0.0005 | 0.0012 | 0 | 0.0057 | 0 | 0.0035 | 0.0008 | 0.001 | 0.0001 | 0.0091 | 0 | 0.0001 | 0 | 0.0006 | 0 |
Punctate Nuclear | 0.0175 | 0.0002 | 0.0016 | 0.0006 | 0.0045 | 0.0003 | 0.0048 | 0.0001 | 0.0015 | 0.0005 | 0.0044 | 0.0004 | 0.0054 | 0 | 0.0013 | 0.0001 | 0.0043 | 0.0005 |
Vacuole | 0.0102 | 0.0001 | 0.0018 | 0.0086 | 0.0079 | 0.0021 | 0.0075 | 0.0012 | 0.0028 | 0.0004 | 0.0095 | 0.0057 | 0.0032 | 0 | 0.0007 | 0.0111 | 0.0079 | 0.0002 |
Vacuole Periphery | 0.0039 | 0 | 0.0012 | 0.0024 | 0.0056 | 0.0004 | 0.0026 | 0 | 0.0017 | 0.0005 | 0.0055 | 0.0014 | 0.0021 | 0 | 0.0013 | 0.0068 | 0.0194 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 151.6969 | 171.2143 | 200.1293 | 243.3358 | 224.1512 | 201.5859 | 208.7333 | 259.5311 | 282.2806 | 209.8693 |
Translational Efficiency | 6.5399 | 5.624 | 4.6306 | 4.9884 | 4.0475 | 4.68 | 4.4728 | 3.9577 | 3.2488 | 3.6672 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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