Standard name
Human Ortholog
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0.11 0 0 0 0 0 0
Cytoplasm 0.59 0.66 0.69 0.73 0.79 0.72 0.65 0.61 0.63 0.62 0.57 0.59 0.72 0.82 0.82 0.87 0.86 0.82 0.11 0.24 0.17 0.16 0.23 0.26
Endoplasmic Reticulum 0.31 0.32 0.19 0.07 0 0 0 0 0 0 0 0 0.23 0.17 0.16 0 0 0 0.7 0.53 0.63 0.51 0.34 0.39
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.26 0.27 0.4 0.53 0.53 0.57 0.51 0.62 0.56 0 0 0 0 0.05 0.07 0.06 0.07 0.09 0.16 0.19 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 2 3 1 1 0 1 0 0 0 0 0 1 1 3 4 3 2 14 16 6 9 9 10
Bud 2 3 8 3 8 10 14 19 13 12 32 22 2 0 1 0 3 0 3 2 2 0 3 3
Bud Neck 0 0 1 0 0 1 0 0 0 1 1 0 0 2 0 4 2 0 7 8 4 7 5 5
Bud Site 0 0 0 0 0 1 1 1 1 1 1 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 4 6 9 6 7 6 6 6 3 15 8 9 10 22 14 12 16 9 8 6 5 10 12
Cytoplasm 250 168 154 155 202 300 326 308 266 268 351 376 186 277 309 183 139 121 61 98 48 46 67 99
Endoplasmic Reticulum 130 83 42 15 4 7 5 7 6 3 3 10 61 58 59 8 6 6 391 213 182 150 101 146
Endosome 8 0 1 4 1 1 1 1 2 2 0 0 0 3 0 1 3 1 3 4 1 4 6 4
Golgi 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 4 2 0 3 0 1
Mitochondria 12 4 28 54 70 166 265 266 243 218 379 358 1 0 1 6 8 10 35 29 27 45 57 48
Nucleus 0 1 0 1 2 1 3 3 2 4 3 3 0 0 0 1 1 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 2 2 0 3 2 2 2 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 0 1 0 0 5 3 1 3 1 2 1 0 1 1 1 0 0 0 0 1 1 1
Vac/Vac Membrane 46 9 4 4 8 11 14 4 5 19 21 6 11 7 16 3 5 7 19 16 6 15 26 36
Unique Cell Count 424 256 223 211 257 419 504 502 424 430 614 634 260 339 375 211 162 147 556 407 289 293 297 377
Labelled Cell Count 464 276 247 248 302 508 643 618 550 536 810 795 272 358 412 227 183 166 556 407 289 293 297 377


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.2 4.2 4.4 4.3 4.0 3.6 3.7 3.5 3.4 3.3 3.4 6.3 6.0 6.0 6.0 5.8 6.2 6.8 6.5 6.8
Std Deviation (1e-4) 0.6 0.6 0.9 1.2 1.5 1.7 1.2 1.2 1.2 1.0 0.7 0.6 0.8 0.8 0.7 0.8 1.0 1.2 1.3 1.2 1.2
Intensity Change (Log2) – – – 0.07 0.01 -0.06 -0.24 -0.18 -0.25 -0.32 -0.36 -0.31 0.57 0.51 0.51 0.51 0.47 0.55 0.69 0.63 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.5 0.2 1.8 2.0 2.2 3.3
Cytoplasm 1.0 2.4 0.7 -1.1 -2.0 -1.6 -1.7 -3.1 -2.6 0.6 3.5 3.8 4.4 3.8 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.2 -0.5 -1.0 -4.9 -4.4 -4.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.6 -6.7 -6.8 -3.8 -2.5 -1.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.9 0.7 0.8 0 0 1.7 1.2 0 1.5 0 1.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5778 1.6801 1.289 1.7462 1.0176 1.4709 4.023 3.4874 3.7942 2.9408 3.658 3.5429 1.0949 1.4306 1.1889 1.0465 1.1134 1.1378
Actin 0.0251 0.0025 0.0272 0.0544 0.004 0.002 0.1372 0.0107 0.0023 0.0013 0.088 0.0032 0.003 0.0009 0.0044 0.0006 0.0011 0.0011
Bud 0.001 0.0003 0.0002 0.0006 0.0038 0 0.0006 0.0001 0.0001 0.0002 0.0005 0.0001 0 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0019 0.0007 0.001 0.0082 0.0024 0.0008 0.001 0.0012 0.0017 0.0197 0.003 0.0023 0.0002 0.0002 0.0013 0.0008 0.0007 0.0018
Bud Periphery 0.0007 0.0003 0.0006 0.001 0.0006 0.0001 0.0008 0.0003 0.0002 0.0004 0.0016 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0017 0.0231 0.0028 0.01 0.0026 0.0001 0.0032 0.0052 0.0064 0.0017 0.0072 0.0001 0.0007 0.0033 0.0017 0.0003 0.0003 0.0002
Cell Periphery 0.0012 0.0023 0.0015 0.0035 0.0007 0.0005 0.0035 0.0033 0.0042 0.0026 0.0095 0.002 0.002 0.0017 0.0019 0.0009 0.0013 0.0006
Cytoplasm 0.2989 0.4645 0.4036 0.305 0.3955 0.4655 0.2379 0.3835 0.2907 0.121 0.3838 0.4213 0.3074 0.355 0.3662 0.4366 0.38 0.4365
Cytoplasmic Foci 0.0216 0.0157 0.0076 0.0256 0.0457 0.012 0.0106 0.0164 0.0282 0.0118 0.027 0.0164 0.0066 0.0136 0.008 0.0072 0.0189 0.0086
Eisosomes 0.0008 0.0002 0.0007 0.0008 0.0001 0.0001 0.0007 0.001 0.0003 0.0003 0.0015 0.0001 0.0009 0.0002 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.2778 0.3113 0.302 0.2311 0.259 0.2621 0.4068 0.3584 0.4118 0.7447 0.2406 0.3406 0.4236 0.3642 0.3925 0.4355 0.3557 0.3605
Endosome 0.0392 0.013 0.0125 0.038 0.0131 0.0147 0.0245 0.0353 0.0105 0.0102 0.0173 0.0165 0.009 0.0295 0.0119 0.0172 0.0185 0.0147
Golgi 0.0086 0.0023 0.0048 0.0123 0.0016 0.0009 0.0127 0.0032 0.0017 0.0015 0.0065 0.0044 0.0009 0.0017 0.0014 0.0006 0.001 0.0007
Lipid Particles 0.0135 0.0068 0.0053 0.0235 0.0024 0.0045 0.0201 0.0142 0.0192 0.0044 0.0113 0.0064 0.0073 0.0079 0.0049 0.0039 0.0074 0.0033
Mitochondria 0.0033 0.0005 0.0041 0.003 0.0015 0.0006 0.0056 0.0005 0.0003 0.0013 0.0038 0.0019 0.0015 0.0011 0.0009 0.001 0.0013 0.0008
None 0.2296 0.1364 0.202 0.2291 0.2346 0.2115 0.0751 0.1455 0.2046 0.0444 0.1757 0.1727 0.2194 0.1837 0.1867 0.0534 0.1901 0.1477
Nuclear Periphery 0.0342 0.0095 0.0113 0.0188 0.0116 0.0105 0.0449 0.0078 0.0059 0.026 0.0058 0.0046 0.0088 0.018 0.0095 0.0168 0.0082 0.008
Nucleolus 0.0012 0.0003 0.0005 0.001 0.003 0.0003 0.0002 0.0002 0.0003 0.0002 0.0006 0.0001 0.0002 0.0003 0.0001 0.0006 0.0006 0.0002
Nucleus 0.0114 0.0032 0.0036 0.006 0.0082 0.0066 0.0019 0.0037 0.0014 0.0022 0.0038 0.0014 0.0024 0.0066 0.003 0.0108 0.005 0.0095
Peroxisomes 0.007 0.0003 0.001 0.0065 0.0003 0.0002 0.0039 0.0004 0.0008 0.0003 0.0035 0.0008 0.0004 0.0002 0.0002 0.0003 0.0003 0.0001
Punctate Nuclear 0.0118 0.0018 0.0029 0.0174 0.0039 0.0025 0.0026 0.0013 0.0058 0.0009 0.0021 0.001 0.0011 0.0023 0.0007 0.0068 0.0058 0.0009
Vacuole 0.0077 0.0047 0.004 0.0033 0.0048 0.0039 0.0044 0.0074 0.0032 0.004 0.0064 0.0035 0.0041 0.0083 0.0042 0.0058 0.0031 0.0039
Vacuole Periphery 0.002 0.0004 0.0009 0.001 0.0005 0.0005 0.0018 0.0005 0.0002 0.0008 0.0006 0.0003 0.0003 0.0009 0.0004 0.0008 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.6099 25.8772 28.2382 24.2474 25.3889 23.8258 25.3948 24.4112 25.2138 27.6112
Translational Efficiency 1.1768 1.2927 0.9934 1.021 1.3581 2.0632 1.58 1.2863 1.6575 1.5171

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
Localization
Cell Percentages ER (80%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Vph2

Vph2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vph2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available