Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 5 | 0 | 2 | 0 | 2 | 4 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 8 | 6 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 6 | 3 | 1 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 24 | 2 | 2 | 4 | 22 | 13 | 23 | 19 | 12 | 19 | 24 | 29 | 75 | 92 | 28 | 28 | 33 | 3 | 8 | 1 | 9 | 9 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 15 | 20 | 0 | 1 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 5 | 15 | 1 | 10 | 6 | 4 | 2 | 0 | 1 | 2 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 |
Mitochondria | 3 | 35 | 0 | 4 | 43 | 23 | 16 | 22 | 151 | 37 | 81 | 152 | 3 | 1 | 4 | 14 | 11 | 5 | 0 | 6 | 0 | 6 | 6 | 8 |
Nucleus | 260 | 367 | 121 | 145 | 130 | 170 | 153 | 198 | 173 | 159 | 203 | 245 | 333 | 394 | 367 | 247 | 262 | 221 | 271 | 378 | 138 | 279 | 244 | 275 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 1 | 1 | 0 | 1 | 2 | 2 | 5 | 1 | 4 | 1 | 3 | 16 | 1 | 0 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 8 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 4 | 3 | 1 | 0 | 4 | 9 | 4 |
Vac/Vac Membrane | 26 | 18 | 3 | 2 | 6 | 11 | 12 | 13 | 10 | 11 | 15 | 33 | 10 | 36 | 29 | 33 | 56 | 47 | 13 | 23 | 5 | 30 | 50 | 76 |
Unique Cell Count | 289 | 414 | 126 | 151 | 153 | 212 | 187 | 244 | 259 | 198 | 274 | 375 | 375 | 502 | 499 | 306 | 346 | 302 | 303 | 435 | 151 | 351 | 348 | 406 |
Labelled Cell Count | 302 | 445 | 126 | 156 | 193 | 232 | 205 | 262 | 366 | 228 | 324 | 498 | 381 | 514 | 518 | 338 | 386 | 336 | 303 | 435 | 151 | 351 | 348 | 406 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 5.5 | 6.9 | 6.3 | 4.7 | 5.7 | 5.8 | 5.7 | 4.6 | 5.1 | 5.1 | 4.8 | 6.9 | 6.6 | 6.3 | 6.2 | 7.8 | 7.6 | 7.1 | 7.4 | 7.4 |
Std Deviation (1e-4) | 1.3 | 0.9 | 1.0 | 0.9 | 1.2 | 1.4 | 1.3 | 1.2 | 1.0 | 1.0 | 1.0 | 0.9 | 1.3 | 1.3 | 1.5 | 1.6 | 1.8 | 2.0 | 1.7 | 1.8 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.55 | -0.27 | -0.26 | -0.27 | -0.59 | -0.42 | -0.43 | -0.51 | -0.01 | -0.07 | -0.14 | -0.14 | 0.19 | 0.15 | 0.05 | 0.1 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 3.0 | 2.2 | 2.8 | 2.3 | 1.9 | 2.2 | 2.1 | 2.5 | 4.1 | 4.7 | 2.8 | 2.6 | 3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.8 | 3.4 | 3.5 | 0 | 5.2 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | -3.1 | -4.1 | -3.7 | -3.9 | -6.3 | -4.0 | -5.2 | -6.7 | -2.4 | -4.6 | -5.5 | -4.1 | -5.0 | -5.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 1.3 | 1.6 | 1.3 | 0 | 1.4 | 1.4 | 2.4 | 0 | 2.0 | 1.6 | 2.9 | 4.0 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.7334 | 6.8496 | 6.7592 | 6.857 | 6.3277 | 6.9998 | 8.4433 | 9.5338 | 8.2776 | 8.899 | 8.0782 | 8.6599 | 4.5933 | 5.1024 | 4.7825 | 4.8911 | 4.7421 | 5.0634 |
Actin | 0.0313 | 0 | 0.0059 | 0.0001 | 0.0177 | 0.0006 | 0.0069 | 0 | 0.0045 | 0 | 0.0011 | 0.0001 | 0.0166 | 0 | 0.0055 | 0.0006 | 0.0002 | 0 |
Bud | 0.0005 | 0 | 0.0005 | 0 | 0.001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0011 | 0.0002 | 0.0001 | 0 | 0 |
Bud Neck | 0.0027 | 0.0001 | 0.001 | 0.0001 | 0.0022 | 0.0007 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0148 | 0.0004 | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0008 |
Bud Periphery | 0.0008 | 0 | 0.001 | 0 | 0.003 | 0.0001 | 0.0007 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0 |
Bud Site | 0.0027 | 0.0001 | 0.005 | 0 | 0.0018 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0 | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0045 | 0.0001 | 0.0002 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0004 | 0 | 0.0012 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0071 | 0.0003 | 0.0039 | 0.0001 | 0.003 | 0.0003 | 0.0011 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0021 | 0.0004 | 0.0018 | 0.0002 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0066 | 0 | 0.002 | 0.0019 | 0.0074 | 0.0002 | 0.0014 | 0 | 0.0006 | 0 | 0.0027 | 0.0009 | 0.0034 | 0 | 0.0024 | 0.0003 | 0.0073 | 0 |
Eisosomes | 0.0006 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0 | 0.0024 | 0.0003 | 0.0054 | 0.0006 | 0.0034 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0071 | 0 | 0.0007 | 0.0011 | 0.0002 | 0 |
Endosome | 0.0093 | 0 | 0.0053 | 0.0154 | 0.0109 | 0.0008 | 0.0103 | 0.0002 | 0.0018 | 0 | 0.0125 | 0.0007 | 0.011 | 0 | 0.0058 | 0.0024 | 0.0085 | 0 |
Golgi | 0.0032 | 0 | 0.0025 | 0.0076 | 0.005 | 0.0002 | 0.0039 | 0 | 0.001 | 0 | 0.01 | 0.0002 | 0.0045 | 0 | 0.0056 | 0.0007 | 0.0099 | 0 |
Lipid Particles | 0.0041 | 0 | 0.0025 | 0.0269 | 0.0174 | 0.0007 | 0.0085 | 0 | 0.0015 | 0 | 0.0006 | 0.0009 | 0.0053 | 0 | 0.0009 | 0.0087 | 0.0067 | 0 |
Mitochondria | 0.007 | 0.0001 | 0.0045 | 0.0009 | 0.0374 | 0.0004 | 0.0048 | 0 | 0.0025 | 0.0001 | 0.002 | 0.0001 | 0.0035 | 0.0002 | 0.0021 | 0.0029 | 0.0034 | 0.0001 |
None | 0.0038 | 0 | 0.0052 | 0 | 0.0048 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0014 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0 |
Nuclear Periphery | 0.0254 | 0.002 | 0.009 | 0.0023 | 0.0104 | 0.002 | 0.0181 | 0.002 | 0.0034 | 0.0014 | 0.0008 | 0.0003 | 0.0263 | 0.0014 | 0.0068 | 0.0257 | 0.0028 | 0.0007 |
Nucleolus | 0.0068 | 0.0068 | 0.0058 | 0.0012 | 0.009 | 0.014 | 0.0061 | 0.0052 | 0.0057 | 0.0108 | 0.0088 | 0.0091 | 0.0086 | 0.0056 | 0.0023 | 0.0065 | 0.0168 | 0.0128 |
Nucleus | 0.8606 | 0.9896 | 0.9333 | 0.94 | 0.8511 | 0.9781 | 0.9233 | 0.9877 | 0.9729 | 0.987 | 0.9429 | 0.9848 | 0.8955 | 0.9895 | 0.9561 | 0.9444 | 0.9245 | 0.9827 |
Peroxisomes | 0.007 | 0 | 0.0034 | 0.0002 | 0.0026 | 0.0001 | 0.0007 | 0 | 0.0003 | 0 | 0.0004 | 0.0011 | 0.0015 | 0 | 0.0009 | 0.0002 | 0.0168 | 0 |
Punctate Nuclear | 0.0136 | 0.0008 | 0.0033 | 0.0004 | 0.0033 | 0.0008 | 0.0028 | 0.0005 | 0.0014 | 0.0001 | 0.0013 | 0.0011 | 0.0068 | 0.0011 | 0.0025 | 0.0014 | 0.0007 | 0.0025 |
Vacuole | 0.0016 | 0.0001 | 0.0021 | 0.001 | 0.0031 | 0.0002 | 0.004 | 0.0033 | 0.0006 | 0.0003 | 0.0006 | 0 | 0.0018 | 0.0001 | 0.0002 | 0.0014 | 0.0006 | 0.0001 |
Vacuole Periphery | 0.0016 | 0 | 0.0009 | 0.0014 | 0.0017 | 0.0001 | 0.0016 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0017 | 0 | 0.0002 | 0.0022 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.7678 | 51.4072 | 30.1992 | 27.9849 | 24.8532 | 21.553 | 30.3204 | 31.0618 | 27.8499 | 19.7452 |
Translational Efficiency | 0.9823 | 0.6996 | 1.0845 | 1.0209 | 0.9899 | 0.7004 | 1.2461 | 1.095 | 0.9348 | 1.2426 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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