Standard name
Human Ortholog
Description Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.96 0.99 0.96 0.92 0.92 0.9 0.9 0.81 0.77 0.73 0.71 0.99 0.99 0.99 0.97 0.97 0.94 0.93 0.93 0.94 0.95 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.09 0.16 0.07 0.39 0.32 0.42 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 2 1 0 6 1 6 5 9 7 6 8 0 0 0 1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 4 5 1 6 2 7 2 8 6 6 1 5 7 6 7 6 0 0 0 0 0 0
Cytoplasm 161 240 268 173 146 224 206 390 231 228 223 260 256 361 379 332 204 188 217 240 252 166 243 254
Endoplasmic Reticulum 1 3 2 0 2 5 1 1 1 0 1 0 1 0 3 11 10 10 0 0 2 0 1 2
Endosome 1 0 0 0 0 0 1 1 0 0 2 1 0 0 0 1 1 1 1 1 1 0 0 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 1 1 1 0 0 0
Mitochondria 3 5 0 5 16 23 36 31 112 93 127 144 0 1 1 5 2 0 0 5 2 0 0 2
Nucleus 5 1 0 1 4 2 7 6 5 2 3 3 1 1 2 3 3 2 4 0 0 1 1 0
Nuclear Periphery 6 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 5 0 1 7 1 2 0 17 3 4 6 27 1 1 0 1 1 13 1 1 1 0 2 2
Unique Cell Count 179 249 272 180 159 243 228 435 286 295 305 368 258 364 384 341 211 201 234 259 268 175 252 269
Labelled Cell Count 184 256 277 191 176 263 263 458 364 346 377 449 260 370 392 361 228 222 234 259 268 175 252 269


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 6.5 8.8 8.2 5.8 6.6 5.0 6.4 5.0 5.2 5.1 5.1 9.2 8.8 8.5 9.0 10.8 11.8 9.2 9.5 10.3
Std Deviation (1e-4) 1.6 1.2 1.4 1.8 1.5 1.3 1.5 1.6 1.2 1.2 1.3 1.1 1.8 1.5 1.2 1.3 1.6 2.4 1.8 1.6 1.7
Intensity Change (Log2) – – – -0.09 -0.61 -0.42 -0.81 -0.46 -0.82 -0.76 -0.78 -0.77 0.07 0.01 -0.04 0.03 0.3 0.42 0.07 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1.0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -3.5 -3.5 -4.1 -4.5 -6.8 -7.6 -8.6 -9.2 0.8 0.8 0.2 -1.0 -1.3 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.1 2.8 2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.3 5.2 6.8 4.5 11.5 10.1 12.1 11.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.9 0 0 0 4.3 0 0 0 0 0 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0132 3.0722 2.894 2.1275 1.268 2.5937 7.4609 9.3083 8.0992 7.8815 7.4314 8.1604 4.7567 5.8732 5.451 5.8364 5.2355 5.478
Actin 0.0517 0.0006 0.034 0.0278 0.0226 0.0002 0.0231 0.0022 0.0104 0.0025 0.0075 0.0009 0.0102 0.0008 0.0109 0.0033 0.0059 0.0145
Bud 0.001 0.0003 0.0008 0.0003 0.0003 0.0002 0.0007 0.0002 0.0004 0.0006 0.0018 0.0001 0.0005 0.0001 0.0028 0.0004 0.0004 0.0001
Bud Neck 0.0038 0.0001 0.0018 0.0009 0.0009 0.0006 0.0027 0.0001 0.0006 0.0004 0.0015 0.0006 0.0007 0.0002 0.0005 0.0009 0.0013 0.0022
Bud Periphery 0.0011 0.0001 0.0009 0.0001 0.0003 0.0001 0.001 0.0001 0.0003 0.0003 0.0021 0.0001 0.0011 0 0.0037 0.0003 0.0003 0.0001
Bud Site 0.0049 0.0012 0.0133 0.0002 0.0009 0.0001 0.0013 0.0007 0.0014 0.0005 0.02 0.0001 0.0012 0.0003 0.0013 0.0002 0.0003 0.0003
Cell Periphery 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0006 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.7286 0.9478 0.8368 0.8963 0.8088 0.9682 0.8171 0.9531 0.9169 0.869 0.8658 0.9399 0.8049 0.9433 0.9143 0.908 0.9092 0.9194
Cytoplasmic Foci 0.0349 0.0059 0.0169 0.0083 0.0708 0.0053 0.0181 0.0092 0.0137 0.011 0.0157 0.011 0.0196 0.0038 0.0175 0.0124 0.0176 0.0122
Eisosomes 0.0003 0 0.0004 0.0001 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0078 0.0026 0.0044 0.0194 0.0038 0.0032 0.0101 0.0015 0.0019 0.0026 0.0025 0.0012 0.0099 0.0049 0.0022 0.0027 0.0045 0.0037
Endosome 0.0484 0.0051 0.0206 0.0163 0.0284 0.0092 0.0197 0.0061 0.0055 0.0036 0.0231 0.005 0.0203 0.0014 0.0049 0.0129 0.01 0.0071
Golgi 0.0116 0.0003 0.0046 0.0049 0.0114 0.0003 0.0058 0.0019 0.0017 0.0006 0.0085 0.0013 0.005 0.0002 0.0015 0.0012 0.0126 0.0041
Lipid Particles 0.0114 0.0001 0.0062 0.0005 0.0139 0.0001 0.0029 0.0007 0.0011 0.0002 0.0037 0.0017 0.0095 0.0002 0.0002 0.0002 0.0006 0.0041
Mitochondria 0.0054 0.0002 0.0034 0.0006 0.0013 0.0012 0.0041 0.0003 0.0007 0.0005 0.011 0.0003 0.0045 0.0001 0.0006 0.0004 0.0025 0.0007
None 0.0343 0.03 0.0402 0.014 0.0162 0.0022 0.0726 0.0212 0.0399 0.1017 0.0208 0.0323 0.0826 0.0421 0.032 0.0531 0.0278 0.0222
Nuclear Periphery 0.0124 0.0013 0.0023 0.0053 0.0069 0.0017 0.0047 0.0004 0.0006 0.0009 0.0034 0.002 0.0052 0.0006 0.0005 0.0006 0.001 0.0005
Nucleolus 0.0017 0 0.0012 0 0.0002 0 0.0003 0 0.0001 0.0001 0.0004 0 0.0003 0 0 0 0 0
Nucleus 0.0072 0.0018 0.002 0.002 0.0011 0.0038 0.0018 0.0007 0.0012 0.002 0.0035 0.0008 0.0034 0.0009 0.0013 0.0008 0.0019 0.0012
Peroxisomes 0.0147 0.0001 0.0041 0.0001 0.0042 0 0.0026 0.0006 0.0014 0.001 0.0032 0.0007 0.0125 0.0001 0.004 0.0011 0.0023 0.0065
Punctate Nuclear 0.013 0.0002 0.0026 0.0006 0.0057 0.0002 0.0079 0.0004 0.0008 0.0015 0.0027 0.0009 0.005 0.0005 0.0004 0.0002 0.0008 0.0006
Vacuole 0.0038 0.0018 0.0025 0.0017 0.0014 0.0028 0.0021 0.0003 0.0008 0.0009 0.0018 0.0006 0.002 0.0004 0.001 0.001 0.0008 0.0004
Vacuole Periphery 0.0019 0.0003 0.0006 0.0005 0.0007 0.0009 0.0011 0.0001 0.0001 0.0001 0.0008 0.0002 0.0009 0 0.0002 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1549 14.722 25.4902 24.1047 17.7937 17.8312 23.8787 21.3492 28.625 23.3483
Translational Efficiency 1.7514 1.8974 1.0691 1.1002 1.2227 1.6628 1.179 1.3433 0.9834 1.0823

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Add66

Add66


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Add66-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available