Standard name
Human Ortholog
Description WD-repeat protein involved in ubiquitin-mediated protein degradation; Ub-binding protein with a role in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.69 0.66 0.38 0.39 0.47 0.46 0.44 0.35 0.4 0.33 0.24 0.32 0.76 0.38 0.61 – – – 0.44 0.44 0.4 0.34 0.35 0.29
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0 0 – – – 0.05 0 0 0 0.05 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.08 0.05 0.24 0.25 0.39 0.19 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.1 0.07 0.26 0.23 0.13 0.18 0.24 0.18 0.19 0.17 0.15 0.16 0.05 0 0 – – – 0.23 0.26 0.23 0.17 0.17 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.07
Vac/Vac Membrane 0.34 0.45 0.44 0.44 0.47 0.41 0.33 0.52 0.32 0.41 0.42 0.43 0.22 0.67 0.45 – – – 0.15 0.17 0.21 0.34 0.28 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 2 0 0 0 1 0 0 0 – – – 0 0 2 0 3 2
Bud 0 0 0 2 0 2 7 1 6 8 18 13 0 1 0 – – – 3 5 6 6 11 13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 – – – 1 3 5 0 4 7
Bud Site 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 0 7 2 1 3 3 5 3 3 1 3 1 1 0 – – – 0 0 0 0 0 0
Cytoplasm 96 187 148 114 177 267 289 198 195 150 116 175 164 96 180 – – – 78 155 208 51 88 75
Endoplasmic Reticulum 2 9 24 12 10 12 27 2 9 4 1 11 14 1 4 – – – 8 5 13 4 12 14
Endosome 0 1 0 0 2 2 1 1 1 0 0 6 0 8 2 – – – 6 9 7 2 4 17
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 2 0 0 0
Mitochondria 1 2 2 3 2 41 50 31 120 114 187 101 3 0 0 – – – 0 3 20 1 3 2
Nucleus 14 21 102 66 48 106 157 101 91 75 74 84 10 6 12 – – – 41 91 122 26 42 33
Nuclear Periphery 0 0 0 0 0 0 0 2 0 1 3 4 0 0 0 – – – 6 6 3 1 1 2
Nucleolus 0 0 0 0 0 0 0 2 0 1 1 1 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 1 1 0 1 0 2 1 0 0 0 1 0 0 0 – – – 1 4 9 0 1 17
Vac/Vac Membrane 48 127 173 128 176 234 216 293 156 185 206 230 48 168 135 – – – 26 60 113 51 72 64
Unique Cell Count 140 285 394 291 376 575 652 567 491 454 485 541 216 251 297 176 353 526 151 253 258
Labelled Cell Count 162 348 458 327 417 667 755 639 581 543 607 632 240 281 333 176 353 526 151 253 258


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.4 7.1 6.7 7.4 6.1 6.2 6.5 5.4 5.2 4.9 5.9 7.6 8.2 8.0 – – – 6.9 7.4 7.6
Std Deviation (1e-4) 1.0 1.0 0.7 0.9 1.1 0.9 0.9 1.0 0.8 0.9 0.8 0.9 1.1 1.3 1.3 – – – 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.1 0.05 -0.22 -0.2 -0.13 -0.4 -0.46 -0.54 -0.29 0.1 0.2 0.16 – – – -0.06 0.04 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 3.9 3.1 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.4 2.7 2.7 2.1 -0.8 0.7 -1.4 -4.4 -1.7 9.1 0.2 6.0 – – –
Endoplasmic Reticulum -1.1 -2.3 -3.2 -1.4 -5.4 -3.3 -4.2 -5.2 -3.2 0.2 -3.7 -3.1 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 4.9 5.2 4.2 10.3 10.4 13.7 8.8 0 0 0 – – –
Nucleus -1.0 -4.6 -2.8 -0.7 -3.0 -2.6 -3.3 -3.9 -3.9 -6.5 -7.8 -7.7 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0.8 -1.0 -3.5 2.4 -3.7 -0.9 -0.4 -0.4 -5.3 5.7 0.4 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0546 3.6988 3.288 3.0266 2.5842 3.0798 4.0312 4.9743 4.2986 3.8181 4.2581 4.2799 3.4029 4.0165 3.3124 3.2742 3.0773 3.3819
Actin 0.0223 0.005 0.0016 0.0108 0.0267 0.0023 0.0406 0.0006 0.0042 0.0083 0.0447 0.0081 0.0238 0.0004 0.0056 0.0023 0.0003 0.0054
Bud 0.0006 0.0006 0.0004 0.0005 0.0018 0.0003 0.0008 0.0001 0.0003 0.0023 0.0013 0.0002 0.0006 0.0001 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0029 0.0008 0.0032 0.001 0.0037 0.0072 0.0129 0.0005 0.0012 0.0022 0.0015 0.0029 0.0027 0.0004 0.0004 0.0009 0.0012 0.0021
Bud Periphery 0.001 0.0003 0.0003 0.0005 0.0014 0.0008 0.002 0.0001 0.0004 0.0024 0.0015 0.0004 0.0013 0 0.0002 0.0001 0.0002 0.0003
Bud Site 0.0021 0.0079 0.002 0.0012 0.0172 0.0004 0.0036 0.0007 0.0006 0.0039 0.0064 0.0003 0.0015 0.0003 0.0003 0.0003 0.0008 0.0012
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0005 0 0.0001 0.0003 0.0002 0.0001 0.0003 0 0 0 0.0001 0
Cytoplasm 0.0778 0.1696 0.1148 0.1033 0.1385 0.0654 0.0693 0.1885 0.098 0.0449 0.0785 0.0615 0.0867 0.194 0.0686 0.0703 0.1069 0.0441
Cytoplasmic Foci 0.0118 0.0018 0.0071 0.0157 0.0422 0.0045 0.0248 0.0027 0.0025 0.0134 0.0108 0.0038 0.0098 0.0008 0.0009 0.0167 0.0077 0.0122
Eisosomes 0.0001 0 0 0 0.0003 0 0.0002 0 0 0 0.0003 0 0.0002 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0126 0.0074 0.0039 0.005 0.0094 0.0037 0.0134 0.0046 0.0036 0.0104 0.009 0.0053 0.0078 0.0105 0.0028 0.0052 0.0024 0.0068
Endosome 0.0295 0.0036 0.0084 0.0314 0.0305 0.0174 0.035 0.0033 0.0055 0.0433 0.0385 0.0173 0.0101 0.0027 0.0058 0.027 0.0284 0.0081
Golgi 0.008 0.0004 0.0048 0.0136 0.0223 0.0079 0.0124 0.0002 0.0063 0.0104 0.0208 0.0052 0.0052 0 0.0028 0.0154 0.0034 0.0018
Lipid Particles 0.0048 0.0003 0.0053 0.0038 0.0175 0.0013 0.0161 0.0002 0.009 0.005 0.026 0.0027 0.0085 0.0001 0.0014 0.0051 0.0183 0.0036
Mitochondria 0.0069 0.0011 0.0061 0.0058 0.0455 0.0096 0.0071 0.0003 0.0022 0.0069 0.0656 0.0036 0.011 0.0004 0.0112 0.0058 0.0009 0.0025
None 0.0064 0.0037 0.0122 0.0063 0.0513 0.0016 0.0019 0.0029 0.0006 0.0011 0.0027 0.0025 0.0035 0.0069 0.0007 0.0053 0.0347 0.0004
Nuclear Periphery 0.0837 0.0451 0.0457 0.0496 0.0355 0.0367 0.0533 0.0438 0.0522 0.0384 0.0665 0.0376 0.08 0.0644 0.0484 0.0522 0.0308 0.0481
Nucleolus 0.0035 0.0014 0.0016 0.0005 0.0032 0.0014 0.0015 0.0013 0.0019 0.0013 0.0012 0.0015 0.0009 0.0006 0.0006 0.0006 0.0024 0.0009
Nucleus 0.707 0.7374 0.7695 0.738 0.5145 0.8258 0.6645 0.7388 0.8026 0.7822 0.6007 0.8307 0.7159 0.7094 0.8447 0.7731 0.7485 0.8514
Peroxisomes 0.002 0.0013 0.0031 0.0042 0.0147 0.001 0.0222 0.0004 0.001 0.0106 0.0073 0.0012 0.0155 0 0.0007 0.0018 0.0019 0.0037
Punctate Nuclear 0.0074 0.0096 0.0055 0.004 0.0139 0.0093 0.0103 0.0083 0.0053 0.0085 0.011 0.0109 0.0108 0.0059 0.003 0.0158 0.0056 0.0046
Vacuole 0.0065 0.0022 0.0033 0.0036 0.0069 0.0026 0.0058 0.0021 0.0016 0.003 0.0036 0.0029 0.0028 0.0023 0.0011 0.0012 0.0046 0.002
Vacuole Periphery 0.003 0.0005 0.0009 0.0009 0.0027 0.0007 0.0017 0.0004 0.0007 0.0013 0.002 0.0012 0.0011 0.0006 0.0006 0.0007 0.0008 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3455 29.3892 20.1043 29.5594 19.6476 23.6166 26.3492 29.1437 33.0428 30.3963
Translational Efficiency 1.5358 0.8815 1.3435 1.2782 1.4097 1.6246 1.1498 1.1692 1.1351 1.0419

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description WD-repeat protein involved in ubiquitin-mediated protein degradation; Ub-binding protein with a role in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
Localization
Cell Percentages cytoplasm (33%), ER (14%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Doa1

Doa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Doa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available