Standard name | |
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Human Ortholog | |
Description | Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 11 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 9 | 5 | 2 | 12 | 10 | 3 | 0 | 0 | 1 |
Bud | 6 | 1 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 3 | 4 | 1 | 6 | 5 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud Site | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 12 | – | – | – | – | – | 4 | – | – | – | – | – | – | – | 14 | 18 | 15 | 0 | 7 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 5 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 2 | 6 | 1 | 0 | 3 | 0 | 0 | 0 | 0 |
Endosome | 14 | 7 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 28 | 10 | 1 | 48 | 35 | 6 | 4 | 3 | 5 |
Golgi | 36 | 19 | – | – | – | – | – | 1 | – | – | – | – | – | – | – | 11 | 19 | 3 | 39 | 21 | 15 | 4 | 4 | 5 |
Mitochondria | 19 | 19 | – | – | – | – | – | 5 | – | – | – | – | – | – | – | 17 | 12 | 6 | 3 | 1 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 19 | 15 | – | – | – | – | – | 6 | – | – | – | – | – | – | – | 49 | 42 | 18 | 0 | 1 | 0 | 1 | 1 | 1 |
Peroxisomes | 8 | 2 | – | – | – | – | – | 2 | – | – | – | – | – | – | – | 26 | 13 | 2 | 13 | 3 | 5 | 0 | 8 | 6 |
SpindlePole | 22 | 1 | – | – | – | – | – | 2 | – | – | – | – | – | – | – | 29 | 28 | 1 | 33 | 18 | 4 | 3 | 5 | 7 |
Vac/Vac Membrane | 5 | 13 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | 20 | 11 | 2 | 0 | 6 | 0 | 0 | 1 | 0 |
Unique Cell Count | 111 | 83 | 20 | 153 | 122 | 47 | 164 | 120 | 42 | 22 | 33 | 33 | ||||||||||||
Labelled Cell Count | 161 | 105 | 22 | 210 | 170 | 52 | 164 | 120 | 42 | 22 | 33 | 33 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.1 | 20.6 | – | – | – | – | – | 24.7 | – | – | – | – | – | – | – | 19.5 | 20.4 | 25.9 | – | – | – |
Std Deviation (1e-4) | 7.0 | 5.3 | – | – | – | – | – | 8.3 | – | – | – | – | – | – | – | 9.0 | 10.6 | 14.8 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.3632 | 25.2242 | 25.349 | 24.6951 | 23.1189 | 24.3988 | 22.0214 | 25.9998 | 25.4497 | 24.8156 | 23.4579 | 24.896 | 24.3622 | 27.7269 | 26.9615 | 27.0162 | 25.622 | 26.4739 |
Actin | 0.0268 | 0.0063 | 0.0111 | 0.0008 | 0.0004 | 0.0188 | 0.016 | 0.0044 | 0.0125 | 0.0323 | 0.0113 | 0.015 | 0.032 | 0.0201 | 0.0196 | 0.0005 | 0.002 | 0.0073 |
Bud | 0.0026 | 0.002 | 0.0042 | 0.0005 | 0.0009 | 0.001 | 0.0006 | 0.0004 | 0.0038 | 0.0017 | 0.0137 | 0.0003 | 0.0013 | 0.0024 | 0.0068 | 0.0007 | 0.0012 | 0.0003 |
Bud Neck | 0.001 | 0.0005 | 0.0009 | 0.0003 | 0.0003 | 0.0019 | 0.0004 | 0.0003 | 0.0004 | 0.0008 | 0.0007 | 0.0015 | 0.0009 | 0.0006 | 0.0006 | 0.0004 | 0.0007 | 0.0009 |
Bud Periphery | 0.0014 | 0.0006 | 0.0018 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0022 | 0.0006 | 0.0069 | 0.0002 | 0.0011 | 0.0008 | 0.0033 | 0.0003 | 0.0007 | 0.0001 |
Bud Site | 0.004 | 0.0017 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0014 | 0.0003 | 0.0007 | 0.0004 | 0.0032 | 0.0002 | 0.0038 | 0.0078 | 0.0017 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0724 | 0.1533 | 0.0671 | 0.1117 | 0.1848 | 0.0818 | 0.0474 | 0.0965 | 0.0584 | 0.0422 | 0.1226 | 0.0491 | 0.0377 | 0.1043 | 0.0476 | 0.129 | 0.0794 | 0.0446 |
Cytoplasmic Foci | 0.0554 | 0.0903 | 0.0625 | 0.0557 | 0.0498 | 0.052 | 0.0705 | 0.0931 | 0.0764 | 0.0662 | 0.0903 | 0.0578 | 0.0711 | 0.0974 | 0.0646 | 0.053 | 0.0778 | 0.0625 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.0038 | 0.0024 | 0.0012 | 0.0019 | 0.0116 | 0.0038 | 0.0022 | 0.0014 | 0.0009 | 0.0018 | 0.0028 | 0.0043 | 0.0026 | 0.0033 | 0.0023 | 0.0046 | 0.009 |
Endosome | 0.4125 | 0.4429 | 0.4579 | 0.5414 | 0.5489 | 0.5263 | 0.3934 | 0.4594 | 0.4361 | 0.6195 | 0.516 | 0.4166 | 0.3728 | 0.4074 | 0.4013 | 0.5345 | 0.5922 | 0.3756 |
Golgi | 0.3059 | 0.2125 | 0.2928 | 0.2089 | 0.1487 | 0.2572 | 0.4131 | 0.3128 | 0.3723 | 0.1738 | 0.173 | 0.4362 | 0.405 | 0.3151 | 0.4085 | 0.135 | 0.1992 | 0.4837 |
Lipid Particles | 0.0073 | 0.0062 | 0.0232 | 0.0078 | 0.0002 | 0.0017 | 0.0101 | 0.0026 | 0.0041 | 0.0003 | 0.0024 | 0.0005 | 0.011 | 0.011 | 0.0049 | 0.0273 | 0.001 | 0.0018 |
Mitochondria | 0.0126 | 0.0072 | 0.006 | 0.004 | 0.002 | 0.0065 | 0.0078 | 0.0012 | 0.005 | 0.0012 | 0.0096 | 0.0029 | 0.0096 | 0.0015 | 0.0025 | 0.0027 | 0.0021 | 0.0031 |
None | 0.0009 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0066 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0063 | 0.0016 | 0.001 | 0.0014 | 0.001 | 0.0024 | 0.004 | 0.0006 | 0.0005 | 0.0004 | 0.0013 | 0.0012 | 0.006 | 0.0008 | 0.0014 | 0.0017 | 0.001 | 0.0006 |
Nucleolus | 0.0002 | 0.0041 | 0.0002 | 0 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0 |
Nucleus | 0.0103 | 0.0029 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0028 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0013 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0002 |
Peroxisomes | 0.0008 | 0.001 | 0.001 | 0.0002 | 0.0002 | 0.0006 | 0.0019 | 0.0006 | 0.0009 | 0.001 | 0.0016 | 0.0004 | 0.0026 | 0.0014 | 0.0007 | 0.0003 | 0.0005 | 0.0004 |
Punctate Nuclear | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0465 | 0.0415 | 0.036 | 0.0317 | 0.0333 | 0.016 | 0.009 | 0.0158 | 0.012 | 0.0308 | 0.0219 | 0.0059 | 0.0211 | 0.0113 | 0.018 | 0.0611 | 0.0201 | 0.0036 |
Vacuole Periphery | 0.0236 | 0.0204 | 0.0302 | 0.0333 | 0.0266 | 0.0205 | 0.0082 | 0.0094 | 0.0125 | 0.0274 | 0.0224 | 0.0087 | 0.0137 | 0.0146 | 0.0145 | 0.0496 | 0.0169 | 0.0061 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 128.6001 | 117.0593 | 101.0518 | 123.9257 | 123.107 | 105.8473 | 117.887 | 118.2347 | 119.1157 | 131.5537 |
Translational Efficiency | 1.8858 | 2.0879 | 1.9933 | 1.7561 | 1.8424 | 2.2894 | 1.8911 | 1.7124 | 1.7785 | 1.727 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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