Standard name | |
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Human Ortholog | |
Description | Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 4 | 6 | 7 | 13 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | 2 | 6 | 9 | 12 | 15 | – | 5 | 14 | 3 | 6 | 9 | 8 | 29 | 11 | 15 | 8 | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 2 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 |
Mitochondria | 4 | 1 | 0 | 0 | 0 | 5 | 6 | – | 16 | 9 | 43 | 15 | 1 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 123 | 195 | 189 | 145 | 197 | 200 | 328 | – | 222 | 242 | 250 | 288 | 108 | 150 | 144 | 201 | 170 | 144 | 130 | 199 | 184 | 49 | 99 | 133 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 4 | 5 | – | 1 | 1 | 3 | 4 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 27 | 14 | 6 | 13 | 12 | 41 | 49 | – | 83 | 88 | 146 | 137 | 10 | 16 | 12 | 18 | 15 | 22 | 5 | 9 | 8 | 3 | 9 | 11 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 3 | 3 | 7 | 6 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 9 | 0 | 3 | 2 | 2 | 1 | – | 1 | 1 | 3 | 3 | 1 | 1 | 1 | 5 | 5 | 3 | 2 | 7 | 2 | 1 | 2 | 9 |
Unique Cell Count | 131 | 215 | 192 | 157 | 219 | 241 | 373 | 273 | 310 | 335 | 380 | 123 | 168 | 185 | 228 | 196 | 168 | 145 | 228 | 201 | 59 | 119 | 164 | |
Labelled Cell Count | 156 | 228 | 197 | 170 | 224 | 268 | 410 | 333 | 365 | 457 | 473 | 133 | 186 | 196 | 241 | 212 | 184 | 145 | 228 | 201 | 59 | 119 | 164 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 11.4 | 9.8 | 9.0 | 9.9 | 8.7 | 8.3 | – | 7.8 | 8.2 | 7.4 | 7.6 | 9.3 | 11.2 | 10.2 | 13.3 | 13.7 | 13.2 | 7.5 | 9.3 | 10.1 |
Std Deviation (1e-4) | 1.6 | 3.2 | 1.6 | 1.8 | 2.6 | 2.3 | 1.7 | – | 1.9 | 2.2 | 2.0 | 2.2 | 2.1 | 10.3 | 2.7 | 4.2 | 5.2 | 4.5 | 1.4 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.01 | -0.17 | -0.24 | – | -0.32 | -0.26 | -0.4 | -0.36 | -0.07 | 0.19 | 0.06 | 0.44 | 0.48 | 0.43 | -0.39 | -0.07 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.9 | 2.3 | 2.0 | – | 0 | 2.2 | 0 | 0 | 0 | 0 | 5.2 | 2.2 | 3.2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.4 | 0 | 5.2 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.8 | -3.6 | -5.3 | -4.2 | – | -5.7 | -6.4 | -7.1 | -6.9 | -4.0 | -3.7 | -6.2 | -4.1 | -4.4 | -4.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.1 | 1.2 | 4.6 | 3.8 | – | 7.4 | 7.1 | 9.9 | 8.6 | 2.0 | 2.5 | 1.5 | 2.1 | 2.0 | 3.5 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.519 | 6.0547 | 5.3793 | 5.4645 | 4.1959 | 5.3472 | 5.3878 | 5.795 | 5.4026 | 4.99 | 4.6804 | 5.2775 | 5.3224 | 5.7931 | 5.7937 | 4.9276 | 4.2163 | 4.9799 |
Actin | 0.0113 | 0 | 0.007 | 0 | 0.0376 | 0 | 0.012 | 0 | 0.0009 | 0.0044 | 0.0052 | 0.0001 | 0.009 | 0.0026 | 0.006 | 0.0049 | 0.0936 | 0.0038 |
Bud | 0.0003 | 0 | 0.0055 | 0 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 |
Bud Neck | 0.0005 | 0 | 0.0002 | 0 | 0.0023 | 0.0004 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0101 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0005 |
Bud Periphery | 0.0005 | 0 | 0.0023 | 0 | 0.0012 | 0.0002 | 0.0015 | 0 | 0.0001 | 0.0006 | 0.0012 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0001 |
Bud Site | 0.0004 | 0 | 0.0039 | 0 | 0.0007 | 0.0001 | 0.0017 | 0 | 0.0004 | 0.0005 | 0.0009 | 0.0002 | 0.0082 | 0.0021 | 0.0004 | 0.0013 | 0.0007 | 0.0004 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0009 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0018 | 0 | 0.0011 | 0 | 0.0027 | 0 | 0.002 | 0 | 0.0004 | 0.0008 | 0.0042 | 0.0003 | 0.0222 | 0.0002 | 0.0007 | 0.0016 | 0.0001 | 0.0008 |
Cytoplasmic Foci | 0.0079 | 0 | 0.001 | 0 | 0.0078 | 0 | 0.0101 | 0 | 0.0037 | 0.0007 | 0.0265 | 0.0002 | 0.0368 | 0.0066 | 0.0127 | 0.002 | 0.0034 | 0.0077 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0017 | 0 | 0.0007 | 0 | 0.0031 | 0 | 0.0014 | 0 | 0.0007 | 0.0009 | 0.0046 | 0.0001 | 0.0023 | 0 | 0.0003 | 0.0018 | 0.0001 | 0.0003 |
Endosome | 0.007 | 0 | 0.0022 | 0 | 0.0216 | 0 | 0.0086 | 0 | 0.0032 | 0.0052 | 0.0704 | 0.0002 | 0.026 | 0.0022 | 0.0053 | 0.0083 | 0.0016 | 0.0048 |
Golgi | 0.0014 | 0 | 0.0009 | 0 | 0.0115 | 0 | 0.0043 | 0 | 0.0016 | 0.0008 | 0.018 | 0.0001 | 0.0116 | 0.0015 | 0.0051 | 0.0011 | 0.006 | 0.0029 |
Lipid Particles | 0.0092 | 0 | 0.0008 | 0 | 0.0243 | 0 | 0.0097 | 0 | 0.0019 | 0.0008 | 0.0113 | 0.0001 | 0.032 | 0.0028 | 0.0098 | 0.0015 | 0.0037 | 0.0171 |
Mitochondria | 0.0014 | 0.0001 | 0.0031 | 0 | 0.0077 | 0.0015 | 0.0072 | 0.0001 | 0.0011 | 0.0052 | 0.0123 | 0.0002 | 0.0053 | 0.0004 | 0.0025 | 0.001 | 0.0144 | 0.0022 |
None | 0.0077 | 0 | 0.0007 | 0 | 0.0015 | 0 | 0.0024 | 0 | 0.0003 | 0.0008 | 0.0012 | 0 | 0.0095 | 0.0002 | 0.0025 | 0.0022 | 0.0003 | 0.0006 |
Nuclear Periphery | 0.0054 | 0.0002 | 0.0011 | 0.0001 | 0.0259 | 0.0009 | 0.0109 | 0.0002 | 0.0046 | 0.0246 | 0.0199 | 0.0006 | 0.0106 | 0.0001 | 0.0009 | 0.0058 | 0.0006 | 0.0017 |
Nucleolus | 0.0446 | 0.0246 | 0.0236 | 0.0135 | 0.132 | 0.1209 | 0.0287 | 0.0154 | 0.0148 | 0.0262 | 0.0922 | 0.0851 | 0.0257 | 0.015 | 0.0111 | 0.0146 | 0.148 | 0.0875 |
Nucleus | 0.8757 | 0.9748 | 0.9437 | 0.9862 | 0.6943 | 0.8749 | 0.8799 | 0.9842 | 0.9573 | 0.9209 | 0.7096 | 0.9033 | 0.7518 | 0.9581 | 0.9346 | 0.9455 | 0.7111 | 0.8569 |
Peroxisomes | 0.0085 | 0 | 0.0004 | 0 | 0.0168 | 0 | 0.002 | 0 | 0.0021 | 0.0006 | 0.0045 | 0 | 0.0172 | 0.0053 | 0.0048 | 0.0004 | 0.0118 | 0.008 |
Punctate Nuclear | 0.013 | 0.0002 | 0.0006 | 0.0001 | 0.0048 | 0.0003 | 0.0097 | 0.0001 | 0.0055 | 0.0037 | 0.0085 | 0.0083 | 0.0148 | 0.0009 | 0.0025 | 0.0058 | 0.0017 | 0.003 |
Vacuole | 0.0009 | 0 | 0.0005 | 0 | 0.0011 | 0.0002 | 0.0039 | 0 | 0.0007 | 0.0009 | 0.0044 | 0.0002 | 0.0028 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0007 |
Vacuole Periphery | 0.0006 | 0 | 0.0004 | 0 | 0.0018 | 0.0003 | 0.0013 | 0 | 0.0005 | 0.0018 | 0.0037 | 0 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.3008 | 20.8023 | 24.0119 | 29.0657 | 30.5901 | 13.5669 | 23.0781 | 27.0118 | 29.4349 | 28.7298 |
Translational Efficiency | 1.1054 | 1.2321 | 1.0117 | 1.102 | 0.7985 | 1.9554 | 1.0639 | 0.9155 | 0.9262 | 0.7952 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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