Standard name
Human Ortholog
Description Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.79 0.83 0.8 0.78 – 0.63 0.6 0.49 0.55 0.98 0.99 0.99 0.95 0.94 0.98 0.92 0.93 0.9 0.66 0.68 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.07 0.05 0 0 0 0 0.05 0 0
Endosome 0 0 0 0.05 0.08 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.05 0.16 0.28 – 0.53 0.54 0.55 0.45 0 0 0 0 0 0 0 0 0 0.11 0.09 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 1 2 1 5 – 1 1 0 3 0 0 0 2 2 1 0 4 0 9 16 12
Bud 1 2 0 0 1 2 4 – 2 12 7 1 3 1 4 0 0 0 1 1 0 3 11 14
Bud Neck 0 1 1 0 1 0 1 – 0 0 0 1 0 0 0 0 0 0 1 5 0 2 5 8
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 10 7 0 2 3 0 – 0 2 2 3 17 9 14 10 17 8 0 1 0 1 3 3
Cytoplasm 281 840 252 44 73 163 160 – 88 121 104 89 403 577 559 300 282 261 262 835 243 182 307 348
Endoplasmic Reticulum 2 11 2 0 2 2 4 – 1 2 9 8 1 2 0 21 15 10 0 1 2 13 18 12
Endosome 0 3 0 3 7 13 1 – 2 1 1 2 4 2 7 4 2 1 5 12 1 7 12 12
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 3 3 8 0
Mitochondria 2 5 0 7 4 33 58 – 74 109 118 74 2 1 0 2 5 1 2 8 9 31 39 18
Nucleus 0 4 0 0 1 0 0 – 0 2 1 1 3 0 1 0 4 3 0 3 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 1 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 1
SpindlePole 0 0 1 3 1 1 2 – 0 2 2 4 1 1 0 0 0 2 2 2 0 3 6 6
Vac/Vac Membrane 1 1 1 1 1 4 2 – 2 4 1 3 2 1 3 12 10 0 3 7 3 13 19 12
Unique Cell Count 286 854 258 56 88 203 206 139 202 213 163 412 583 567 317 300 266 285 894 271 277 455 461
Labelled Cell Count 288 880 265 61 95 223 238 170 257 245 190 436 595 588 351 337 288 285 894 271 277 455 461


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.1 6.3 5.9 6.6 5.0 4.8 – 4.3 4.3 4.1 4.3 6.1 6.0 6.2 7.5 8.0 7.2 5.7 5.8 6.3
Std Deviation (1e-4) 0.8 0.7 1.3 1.3 1.4 1.2 0.9 – 0.7 0.9 0.6 0.7 1.4 1.1 1.2 1.1 1.3 1.1 1.6 1.4 1.9
Intensity Change (Log2) – – – -0.1 0.07 -0.34 -0.4 – -0.55 -0.56 -0.61 -0.54 -0.05 -0.08 -0.03 0.25 0.35 0.19 -0.14 -0.12 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 1.0 0 -0.2 0.3 1.7 0.2
Cytoplasm -5.6 -5.0 -6.2 -6.8 – -9.3 -10.3 -12.3 -11.0 0.1 1.5 0.9 -1.8 -2.1 0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.6 2.9 2.3
Endosome 0 0 4.1 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.7 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 2.7 2.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4821 3.1044 3.5236 2.5538 3.2886 2.9009 2.15 1.4398 1.267 2.0036 1.5191 1.4207 0.3529 0.8776 0.8751 0.5938 0.3165 0.6801
Actin 0.0867 0.007 0.0337 0.0022 0.067 0.0094 0.0579 0.0052 0.0013 0.0547 0.0006 0.019 0.0102 0.0021 0.0058 0.0021 0.0005 0.0018
Bud 0.0014 0.0024 0.0046 0.0005 0.0064 0.0016 0.0018 0.009 0.0105 0.017 0.0003 0.009 0.0185 0.0166 0.0376 0.0208 0.0359 0.0114
Bud Neck 0.0072 0.0025 0.0022 0.0004 0.0063 0.0024 0.0201 0.0015 0.0007 0.0023 0.0002 0.0025 0.0004 0.0006 0.0006 0.0007 0.0002 0.0025
Bud Periphery 0.0026 0.0036 0.0081 0.0011 0.0219 0.0013 0.0026 0.0037 0.0105 0.0225 0.0005 0.0188 0.0156 0.0066 0.0216 0.0348 0.0161 0.0099
Bud Site 0.0131 0.0396 0.0282 0.0006 0.0132 0.0003 0.0384 0.0304 0.0091 0.0113 0.0002 0.0011 0.0078 0.0445 0.0239 0.0027 0.0008 0.003
Cell Periphery 0.0015 0.0011 0.0025 0.0004 0.0024 0.0004 0.0013 0.0013 0.0021 0.0009 0.0001 0.0007 0.0011 0.0014 0.001 0.0006 0.0003 0.0005
Cytoplasm 0.1949 0.3318 0.2893 0.4259 0.3209 0.3917 0.3444 0.4289 0.5062 0.2695 0.5527 0.4913 0.3283 0.4329 0.3959 0.3173 0.4095 0.4075
Cytoplasmic Foci 0.0404 0.0197 0.0189 0.0184 0.025 0.0172 0.0878 0.0271 0.0064 0.0384 0.0122 0.0286 0.0186 0.0181 0.012 0.0127 0.0049 0.024
Eisosomes 0.0011 0.0006 0.0006 0.0003 0.0007 0.0001 0.0009 0.0006 0.0001 0.0004 0 0.0001 0.0002 0.0002 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0152 0.0021 0.0033 0.0038 0.0131 0.0016 0.0074 0.0047 0.0054 0.0107 0.0037 0.0028 0.0035 0.0027 0.003 0.0019 0.0004 0.0025
Endosome 0.032 0.0025 0.0209 0.0216 0.0458 0.0015 0.0307 0.0067 0.0039 0.0528 0.0182 0.011 0.0033 0.0084 0.0026 0.0039 0.0011 0.0041
Golgi 0.0137 0.0007 0.0099 0.0024 0.0528 0.0005 0.0208 0.0034 0.0003 0.0334 0.0005 0.0013 0.0009 0.0006 0.0005 0.0003 0.0001 0.0003
Lipid Particles 0.0209 0.0006 0.0086 0.0051 0.0133 0.0002 0.0308 0.0047 0.0007 0.0091 0.0011 0.0008 0.0019 0.0006 0.0004 0.0003 0 0.0005
Mitochondria 0.0189 0.0009 0.0214 0.02 0.1176 0.0004 0.0082 0.0028 0.0012 0.0188 0.0063 0.0028 0.0044 0.0016 0.0022 0.002 0.0007 0.001
None 0.4361 0.5781 0.5152 0.4783 0.2588 0.5644 0.2518 0.4559 0.4174 0.4037 0.2874 0.3925 0.5705 0.4478 0.468 0.5883 0.5265 0.5161
Nuclear Periphery 0.0246 0.0005 0.0089 0.0042 0.0159 0.0004 0.0137 0.0006 0.0034 0.0084 0.0673 0.0009 0.0018 0.0011 0.0022 0.0012 0.0002 0.0018
Nucleolus 0.0036 0.0003 0.0007 0.0015 0.0004 0.0001 0.0021 0.0012 0.0008 0.0007 0.0018 0.0012 0.0013 0.0012 0.0014 0.0007 0.0001 0.0007
Nucleus 0.032 0.001 0.0027 0.0028 0.0019 0.0022 0.0082 0.0013 0.0113 0.004 0.0179 0.0041 0.0019 0.0045 0.0102 0.0029 0.0008 0.0037
Peroxisomes 0.0181 0.0013 0.0134 0.001 0.0096 0.0031 0.0468 0.0032 0.0001 0.0228 0.0001 0.0023 0.0014 0.0005 0.0008 0.0006 0.0001 0.0003
Punctate Nuclear 0.029 0.0015 0.0029 0.002 0.0013 0.0004 0.0176 0.0055 0.0005 0.003 0.0011 0.003 0.0038 0.0042 0.0071 0.0018 0.0002 0.0059
Vacuole 0.0047 0.0017 0.0029 0.0045 0.003 0.0005 0.005 0.0021 0.0066 0.0107 0.0093 0.005 0.0038 0.0033 0.0024 0.0037 0.0013 0.0021
Vacuole Periphery 0.0024 0.0002 0.001 0.0029 0.0027 0.0001 0.0017 0.0002 0.0013 0.0051 0.0184 0.0013 0.0006 0.0003 0.0004 0.0006 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2237 8.2557 10.6541 13.3113 5.206 6.0196 13.9727 14.2072 13.2338 11.2287
Translational Efficiency 0.4582 0.5336 0.5513 0.3853 0.5755 0.5289 0.4788 0.4406 0.4764 0.4072

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aly1

Aly1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aly1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available