Standard name
Human Ortholog
Description Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.05 0 0.11 0.06 0.05 0.05 0.06 0.05 0 0 0.11 0.2 0.17 0.15 0.16 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0.05 0.06 0.07 0.35 0.46 0.26 0.58 0.44 0.65 0.37 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Nucleus 0.86 0.83 0.88 0.88 0.8 0.79 0.77 0.83 0.77 0.82 0.71 0.83 0.86 0.77 0.78 0.7 0.63 0.69 0.82 0.79 0.79 0.68 0.6 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.15 0 0 0.08 0 0 0 0 0 0 0 0 0.05 0.06 0.13 0.17 0.15 0.12 0.11 0.13 0.16 0.22 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 1 2 6 4 7 6 1 0 1 0 0 0 0 3 4 0 2 3 8
Bud Neck 1 0 3 2 0 1 1 0 0 1 0 1 1 1 0 0 0 0 0 1 0 0 2 1
Bud Site 0 0 0 1 0 8 7 5 10 16 12 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 17 21 7 8 33 22 24 22 26 17 14 15 28 68 47 35 58 31 7 5 1 6 14 7
Endoplasmic Reticulum 0 2 1 0 0 0 0 2 0 0 0 0 2 2 2 21 17 13 0 0 0 0 0 1
Endosome 0 0 1 1 5 2 1 0 1 1 0 0 1 0 3 3 8 2 3 1 1 0 2 1
Golgi 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 3 0 3 1 0 1 1
Mitochondria 141 13 7 12 22 122 232 105 265 151 232 129 5 0 1 13 15 16 3 6 3 2 12 12
Nucleus 407 244 128 163 248 277 383 331 352 282 256 289 214 259 222 161 226 159 401 244 136 104 201 175
Nuclear Periphery 0 0 1 0 0 1 2 0 5 3 2 0 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 1 1 1 3 2 1 0 0 1 0 1 0 0 1 0 1 7 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 4 1 4 2 3 3 3 2 6 2 2 7 3 3 0 10 0 2 5 10 8
Vac/Vac Membrane 12 43 5 6 25 13 14 8 9 4 7 7 3 16 16 30 61 34 57 34 21 23 72 70
Unique Cell Count 476 295 146 186 311 352 499 401 458 343 359 349 249 338 283 230 358 229 493 308 174 153 337 306
Labelled Cell Count 579 324 154 199 334 451 669 483 676 489 533 455 256 349 299 269 392 258 493 308 174 153 337 306


Cytoplasm, Endoplasmic Reticulum, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.4 5.3 5.1 5.1 4.3 4.1 4.8 3.9 4.3 3.8 4.4 5.3 5.6 5.7 6.9 7.1 6.7 5.2 5.7 6.4
Std Deviation (1e-4) 0.5 0.9 0.9 1.2 1.4 1.3 1.0 1.2 1.0 1.4 0.9 1.1 0.9 0.9 0.9 1.8 2.0 1.8 1.1 1.4 1.7
Intensity Change (Log2) – – – -0.06 -0.05 -0.3 -0.37 -0.16 -0.44 -0.3 -0.48 -0.27 -0.0 0.07 0.11 0.37 0.42 0.32 -0.05 0.09 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 2.1 0.6 0 0.3 0.4 0.1 -0.5 -0.2 2.2 4.3 3.5 3.1 3.5 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 2.2 2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.9 0 0 0 0 0 0 0 0 0 0 0.4 -0.3 0.9
Nucleus 0 -2.1 -2.3 -2.9 -1.4 -2.8 -1.5 -3.9 -1.4 -0.5 -2.8 -2.3 -4.0 -5.5 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 0.1 0 0 0 0 0 0 0 0.6 1.0 3.1 4.1 3.5