Standard name
Human Ortholog
Description WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.05 0.05 0 0 – 0 0 0 0 0.11 0.05 0.05 0.05 0 0 0 0 0 0.06 0 0
Bud 0 0 0 0.07 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.09 0.05 0 0 – 0 0 0 0 0 0.06 0 0.16 0.13 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0 0 0 0 – 0 0 0 0.06 0.05 0.13 0.19 0.06 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0.17 0.05 0 0.06 0 – 0 0 0 0 0.11 0 0.08 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0.05 0.11 – 0 0 0 0 0 0.1 0 0 0.09 0 0 0 0 0 0.05 0.05
Golgi 0 0.16 0 0.22 0.16 0 0 – 0 0 0 0 0.12 0.16 0.24 0.5 0.29 0.39 0 0.12 0.05 0.06 0.05 0.07
Mitochondria 0.98 0.79 0.64 0.8 0.65 0.78 0.75 – 0.87 0.88 0.92 0.92 0.82 0.55 0.3 0.11 0.07 0.07 0.82 0.69 0.71 0.69 0.71 0.65
Nucleus 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0.05 0.08 – 0 0 0 0 0 0.11 0 0.07 0.14 0 0.05 0 0.06 0.08 0 0
SpindlePole 0 0 0 0 0 0 0.08 – 0.08 0.06 0.06 0.07 0 0 0.08 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.21 0.11 0.05 0.12 0.08 0.08 – 0.08 0 0 0.05 0.05 0.11 0.13 0.18 0.21 0.25 0 0.07 0.07 0 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 2 4 11 3 1 – 1 1 3 1 9 6 6 12 1 6 3 3 2 2 4 5
Bud 2 0 0 5 4 9 13 – 10 8 14 15 1 3 1 4 9 2 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 1 0 – 2 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 4 – 2 3 1 3 0 0 0 1 3 1 – – – – – –
Cell Periphery 3 7 0 7 10 6 4 – 3 3 0 4 1 7 5 38 26 23 0 0 0 0 0 0
Cytoplasm 1 2 3 3 9 9 11 – 11 12 8 22 4 15 22 15 16 15 0 1 0 0 2 1
Endoplasmic Reticulum 4 10 6 4 8 15 2 – 8 5 12 6 9 2 9 4 7 8 1 1 1 0 0 1
Endosome 1 3 0 1 22 13 42 – 7 7 0 7 0 12 5 6 18 7 6 5 2 1 8 11
Golgi 5 21 1 16 33 5 4 – 1 1 1 0 10 19 28 122 59 75 6 17 3 2 8 17
Mitochondria 167 107 23 59 137 196 285 – 214 235 265 362 70 66 35 26 15 14 141 102 52 28 128 154
Nucleus 0 0 2 1 1 4 7 – 4 5 6 5 0 1 2 0 4 5 0 0 0 0 1 0
Nuclear Periphery 3 2 0 0 0 4 5 – 1 1 3 1 0 0 1 5 0 3 0 0 0 0 0 1
Nucleolus 0 2 0 0 0 1 0 – 2 2 6 0 0 1 0 4 1 0 0 0 0 0 0 0
Peroxisomes 1 1 0 0 10 12 32 – 6 1 2 4 0 13 4 18 29 8 8 2 4 3 2 6
SpindlePole 0 0 1 0 9 7 32 – 19 15 16 27 0 3 9 8 7 6 0 0 0 0 0 0
Vac/Vac Membrane 19 28 4 4 25 20 32 – 19 8 7 18 4 13 15 43 44 48 2 11 5 0 20 28
Unique Cell Count 170 135 36 74 211 250 380 247 268 288 395 85 119 117 243 206 192 173 150 74 41 181 239
Labelled Cell Count 206 189 42 104 279 305 474 310 307 345 477 108 161 142 306 239 221 173 150 74 41 181 239


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.1 6.2 6.9 6.8 5.5 5.4 – 4.6 4.7 4.1 4.1 6.3 7.0 7.1 8.5 8.6 7.9 – 6.4 6.9
Std Deviation (1e-4) 0.8 1.1 0.9 1.6 1.2 1.2 1.0 – 0.9 1.0 0.8 0.9 1.1 1.1 1.3 1.2 1.7 1.3 – 1.0 1.1
Intensity Change (Log2) – – – 0.15 0.13 -0.17 -0.21 – -0.44 -0.41 -0.6 -0.59 0.01 0.17 0.18 0.46 0.47 0.34 – 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0 – – – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 – – – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 – – – – – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – – – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 – – – – – 0 0 0 0 0 0
Endosome 0 0 0 0 – – – – – 0 0 0 0 0 0
Golgi 2.6 0 0 0 – – – – – 0 0 2.8 5.3 3.3 4.2
Mitochondria 1.8 0.1 1.9 1.5 – – – – – 2.2 -0.9 -3.7 -7.8 -8.6 -8.5
Nuclear Periphery 0 0 0 0 – – – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 – – – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – – – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 – – – – – 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 – – – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – – – – – 0 0 1.5 0 0 0
Nucleus 0 0 0 0 – – – – – 0 0 0 0 0 0
Vacuole 0 0 0 0 – – – – – 0 0 0 1.0 1.4 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.886 1.4908 1.0497 0.9705 0.7166 1.2672 1.796 2.0873 2.3165 2.2005 1.3205 2.0079 0.9685 1.1271 1.0251 1.3157 0.673 0.6816
Actin 0.0348 0.0302 0.0142 0.0009 0.0826 0.0144 0.0944 0.0444 0.04 0.0062 0.0061 0.0154 0.0433 0.0091 0.0221 0.0086 0.0082 0.0204
Bud 0.0011 0.003 0.0023 0.0013 0.0013 0.0004 0.0016 0.0042 0.0036 0.0009 0.0015 0.0003 0.0014 0.0052 0.0012 0.0006 0.0013 0.0007
Bud Neck 0.0045 0.0012 0.0058 0.0002 0.002 0.0008 0.0043 0.0021 0.0013 0.0139 0.0011 0.0007 0.0021 0.0008 0.001 0.0004 0.0009 0.0045
Bud Periphery 0.0025 0.0055 0.0049 0.0069 0.0029 0.0009 0.0025 0.0066 0.008 0.0025 0.0041 0.0007 0.0025 0.0037 0.0019 0.0018 0.0064 0.0019
Bud Site 0.0068 0.0388 0.0106 0.0003 0.0035 0.0005 0.0138 0.0579 0.0147 0.0039 0.0021 0.0017 0.0064 0.0148 0.0109 0.0007 0.0008 0.0013
Cell Periphery 0.0012 0.0025 0.004 0.0002 0.0006 0.0004 0.0014 0.0023 0.003 0.0007 0.0004 0.0003 0.0009 0.0012 0.0005 0.0003 0.0004 0.0008
Cytoplasm 0.0039 0.0081 0.0037 0.0002 0.0008 0.001 0.0053 0.0049 0.0066 0.0014 0.0013 0.0004 0.0034 0.0078 0.0055 0.0008 0.0011 0.0022
Cytoplasmic Foci 0.033 0.0304 0.0346 0.0088 0.0201 0.0157 0.055 0.0375 0.0257 0.0388 0.0268 0.0106 0.0339 0.0352 0.0299 0.0086 0.0344 0.017
Eisosomes 0.0015 0.0009 0.0018 0.0001 0.0007 0.0008 0.0014 0.0007 0.002 0.0003 0.0002 0.0005 0.0015 0.0003 0.0008 0.0003 0.0002 0.0007
Endoplasmic Reticulum 0.0026 0.0009 0.0014 0.0001 0.0004 0.0009 0.0015 0.0014 0.0011 0.0003 0.0014 0.0002 0.0036 0.0006 0.0008 0.0005 0.0004 0.0009
Endosome 0.0806 0.083 0.0595 0.0336 0.0367 0.0444 0.0725 0.0709 0.0385 0.0407 0.1148 0.0264 0.0963 0.0611 0.0545 0.0532 0.0696 0.039
Golgi 0.0731 0.0909 0.0386 0.0432 0.0494 0.0625 0.0561 0.0683 0.0507 0.0136 0.0797 0.0463 0.0838 0.0996 0.0705 0.0389 0.039 0.0725
Lipid Particles 0.0339 0.0081 0.0068 0.0064 0.0039 0.0163 0.0347 0.0119 0.0116 0.0131 0.0306 0.0027 0.0449 0.0045 0.0142 0.009 0.0277 0.0132
Mitochondria 0.626 0.6466 0.7548 0.8864 0.7454 0.8104 0.5848 0.6126 0.7311 0.8247 0.6533 0.8489 0.5632 0.7073 0.7386 0.8529 0.7668 0.7772
None 0.0297 0.0009 0.0027 0.0001 0.0002 0.0003 0.0083 0.0052 0.0044 0.0014 0.0004 0.0004 0.0221 0.0006 0.0013 0.0005 0.0004 0.001
Nuclear Periphery 0.003 0.0003 0.0012 0.0001 0.0002 0.0004 0.002 0.0006 0.0004 0.0002 0.0011 0.0001 0.012 0.0002 0.001 0.0012 0.001 0.0011
Nucleolus 0.0022 0.0005 0.0006 0.0001 0.0002 0.0002 0.0039 0.0012 0.0003 0.0003 0.0004 0.0002 0.0019 0.0004 0.0002 0.0004 0.0015 0.0003
Nucleus 0.0014 0.0006 0.0008 0.0001 0.0002 0.0004 0.0022 0.0009 0.0004 0.0002 0.0005 0.0001 0.0048 0.0004 0.0005 0.0004 0.0006 0.0004
Peroxisomes 0.0394 0.0347 0.0317 0.0082 0.0346 0.0207 0.0386 0.0452 0.0473 0.0255 0.0595 0.0412 0.0495 0.0357 0.037 0.0067 0.0291 0.0359
Punctate Nuclear 0.0017 0.0002 0.0007 0 0.0001 0.0005 0.0071 0.0002 0.0003 0.0004 0.0006 0 0.0069 0.0001 0.0012 0.0008 0.0008 0.0028
Vacuole 0.0111 0.009 0.0135 0.0012 0.0066 0.0047 0.0053 0.0166 0.0056 0.0061 0.0104 0.0011 0.008 0.0074 0.0041 0.0064 0.0062 0.0027
Vacuole Periphery 0.0061 0.0039 0.0058 0.0014 0.0077 0.0037 0.0032 0.0045 0.0031 0.0049 0.0037 0.0018 0.0079 0.0041 0.0024 0.0069 0.0034 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1358 12.2531 10.9866 10.9804 13.3287 11.9869 14.583 15.6784 10.4573 15.9409
Translational Efficiency 0.5756 0.4657 0.5427 0.4029 0.4275 0.462 0.3901 0.2986 0.3969 0.4212

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Caf4

Caf4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Caf4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available