Standard name
Human Ortholog
Description hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl2p and Esl1p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; ESL2 has a paralog, ESL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.69 0.74 0.34 0.21 0.4 0.48 0.42 0.47 0.46 0.38 0.41 0.54 0.63 0.67 0.72 0.74 0.78 0.5 0.48 0.59 0.45 0.41 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.05 0 0 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0.2 0.51 0.3 0.48 0.47 0.51 0.62 0.61 0.66 0.68 0 0 0.12 0 0 0 0 0 0.06 0 0 0
Nucleus 0.15 0 0 0.22 0.38 0.15 0.1 0.11 0.1 0.11 0.08 0.08 0 0 0 0 0 0 0.26 0.26 0.07 0.21 0.22 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.33 0.12 0.08 0.25 0.14 0.12 0.15 0.07 0.05 0.1 0.08 0.44 0.35 0.25 0.27 0.36 0.24 0.14 0.16 0.16 0.2 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 2
Bud 0 0 3 3 2 8 8 10 7 9 12 10 0 0 18 0 0 2 5 0 0 4 8 12
Bud Neck 0 0 1 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 4 0 3 2 2 7
Bud Site 0 0 0 0 2 1 1 12 4 6 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 3 0 0 1 1 1 1 0 0 0 0 0 0
Cytoplasm 244 31 125 58 66 112 235 257 149 202 145 176 96 177 201 138 55 117 233 40 143 64 72 138
Endoplasmic Reticulum 1 1 1 0 1 2 2 2 0 0 1 1 0 0 0 6 4 8 1 0 2 0 0 4
Endosome 1 1 0 0 4 4 3 1 0 0 1 0 16 29 15 8 2 14 8 2 1 0 2 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 3
Mitochondria 0 5 34 87 93 135 230 311 196 264 251 291 2 4 35 8 1 2 4 2 13 1 3 7
Nucleus 60 1 2 37 119 43 48 69 31 50 29 35 3 6 2 1 0 0 121 21 16 29 39 42
Nuclear Periphery 0 0 0 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 2 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 1 3 1 0 0 0 0 1 3 0 0 0 1 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1
SpindlePole 0 0 0 0 0 0 0 5 1 3 2 1 0 0 0 0 0 0 9 0 10 4 2 12
Vac/Vac Membrane 147 15 21 13 76 39 56 92 23 22 37 35 78 97 76 52 27 36 67 13 39 28 36 64
Unique Cell Count 390 45 170 170 310 279 485 606 314 436 382 430 177 279 302 191 74 150 468 84 243 142 177 316
Labelled Cell Count 453 54 187 198 365 345 585 763 412 558 485 556 195 314 352 216 90 180 468 84 243 142 177 316


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 3.8 4.0 4.3 4.0 3.8 3.8 3.3 3.4 3.4 3.1 5.5 5.7 4.9 16.9 24.0 26.5 5.3 5.4 6.1
Std Deviation (1e-4) 0.6 1.5 0.5 1.0 1.1 1.0 1.2 0.9 1.0 1.0 1.1 0.8 1.6 1.7 1.8 32.9 44.9 48.8 0.9 1.4 1.9
Intensity Change (Log2) – – – 0.07 0.2 0.07 0.0 -0.0 -0.19 -0.15 -0.15 -0.29 0.56 0.59 0.39 2.17 2.68 2.82 0.49 0.51 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -7.3 -11.2 -6.9 -5.7 -7.2 -5.5 -6.0 -7.7 -7.2 -3.7 -2.2 -1.6 -0.3 0.1 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 4.0 4.3 3.0 0 0 4.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.8 -6.9 -2.5 -4.7 -3.8 -5.3
Nucleus 6.0 9.0 4.9 3.7 4.1 3.6 4.1 3.0 3.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.4 3.2 0.5 -0.3 0.9 -1.8 -3.1 -0.9 -1.6 6.5 5.2 3.3 3.5 4.4 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.661 2.9079 2.805 2.2069 2.2825 2.9467 2.2394 3.0068 2.7438 2.615 2.8991 2.5873 2.6526 2.1134 2.104 1.8964 2.2478 2.1425
Actin 0.0005 0.0008 0.0006 0.0004 0.0007 0.001 0.0152 0.001 0.0206 0.0263 0.0023 0.0082 0.001 0.0003 0.0051 0.0023 0.001 0.0006
Bud 0.0001 0.0001 0.0002 0.0002 0.0003 0.0002 0.0006 0.0003 0.0019 0.001 0.0016 0.0004 0.0002 0.0001 0.0011 0.0001 0.0001 0.0001
Bud Neck 0.0009 0.0019 0.0013 0.0017 0.0033 0.0085 0.0008 0.0006 0.0012 0.0014 0.0028 0.004 0.0017 0.0003 0.01 0.0009 0.0003 0.003
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0005 0.0002 0.0013 0.0016 0.0017 0.0004 0.0001 0 0.0034 0 0 0.0001
Bud Site 0.0005 0.0025 0.0015 0.0008 0.0006 0.0006 0.0073 0.0016 0.0038 0.0018 0.0098 0.0005 0.0007 0.0011 0.0057 0.0002 0.0003 0.0002
Cell Periphery 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0042 0 0.0001 0
Cytoplasm 0.5528 0.4921 0.4596 0.3626 0.4904 0.426 0.5694 0.6187 0.5606 0.4739 0.3178 0.5429 0.3761 0.7433 0.4844 0.5359 0.541 0.5638
Cytoplasmic Foci 0.0028 0.0043 0.0022 0.0096 0.0041 0.0023 0.0294 0.0042 0.0128 0.019 0.0388 0.0091 0.1496 0.0031 0.0107 0.0114 0.006 0.0032
Eisosomes 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0.0001 0.0002 0 0 0 0 0.001 0 0 0
Endoplasmic Reticulum 0.0153 0.0151 0.0062 0.0082 0.0086 0.0048 0.0109 0.0077 0.0044 0.0046 0.0054 0.0109 0.0203 0.0057 0.0109 0.004 0.0127 0.0039
Endosome 0.0043 0.0054 0.0033 0.0192 0.0114 0.0033 0.0346 0.0032 0.0157 0.0116 0.0922 0.0128 0.1652 0.0017 0.0078 0.0043 0.0048 0.0021
Golgi 0.0001 0.0002 0.0001 0.0004 0.0002 0.0001 0.0078 0.0003 0.0049 0.0121 0.031 0.0035 0.0025 0.0001 0.0018 0.0004 0.0002 0.0001
Lipid Particles 0.0004 0.0009 0.0001 0.0017 0.0003 0.0001 0.0186 0.0002 0.002 0.0044 0.0079 0.0028 0.0048 0.0001 0.0054 0.0003 0.0004 0.0001
Mitochondria 0.0004 0.0003 0.0004 0.0007 0.0004 0.0005 0.0041 0.0005 0.0053 0.0311 0.0064 0.0057 0.0013 0.0002 0.0026 0.0005 0.0004 0.0003
None 0.0924 0.1288 0.0726 0.1641 0.1449 0.0564 0.0739 0.1055 0.08 0.0171 0.0775 0.0513 0.2113 0.0897 0.1221 0.1324 0.3282 0.2234
Nuclear Periphery 0.0249 0.0134 0.0151 0.0128 0.0163 0.0151 0.016 0.0077 0.0102 0.012 0.0318 0.0105 0.0144 0.0063 0.0139 0.0088 0.0089 0.0056
Nucleolus 0.002 0.0017 0.0012 0.0024 0.0023 0.0026 0.003 0.0004 0.001 0.0006 0.0018 0.0007 0.0007 0.0002 0.007 0.0004 0.0004 0.0005
Nucleus 0.2883 0.3188 0.4247 0.3844 0.3028 0.4528 0.1741 0.2406 0.2611 0.3459 0.3386 0.3138 0.0056 0.1429 0.2619 0.2828 0.0895 0.1856
Peroxisomes 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0136 0.0004 0.0019 0.0188 0.0077 0.0084 0.0102 0.0001 0.001 0.0009 0.0001 0.0001
Punctate Nuclear 0.0103 0.0089 0.0074 0.0204 0.0094 0.0233 0.0077 0.0053 0.0081 0.0143 0.0064 0.0117 0.0138 0.0035 0.0291 0.013 0.0043 0.006
Vacuole 0.0034 0.0037 0.0028 0.0088 0.0032 0.0017 0.0108 0.0013 0.0022 0.0015 0.0142 0.0016 0.0172 0.0011 0.0099 0.0011 0.001 0.0012
Vacuole Periphery 0.0003 0.0003 0.0003 0.0008 0.0003 0.0003 0.0011 0.0002 0.0007 0.0006 0.0038 0.0005 0.0031 0.0001 0.0009 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0688 13.4706 11.6277 18.442 14.1546 13.5237 19.3183 20.8241 19.145 23.1347
Translational Efficiency 0.5909 0.7387 0.777 0.4877 0.5885 0.8034 0.5728 0.5238 0.6092 0.4655

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl2p and Esl1p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; ESL2 has a paralog, ESL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (21%), nucleus (10%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Esl2

Esl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Esl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available