Standard name | |
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Human Ortholog | |
Description | Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 1 | 1 | 3 |
Bud | 0 | 1 | 0 | 0 | 2 | 5 | – | 8 | 14 | 10 | 25 | 13 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 9 | 3 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 6 | 3 | 2 | 3 | – | 6 | 5 | 11 | 8 | 9 | 7 | 2 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 347 | 227 | 599 | 363 | 539 | 564 | – | 677 | 421 | 484 | 421 | 498 | 216 | 272 | 284 | 140 | 149 | 73 | 337 | 219 | 542 | 342 | 376 | 352 |
Endoplasmic Reticulum | 3 | 1 | 2 | 1 | 0 | 1 | – | 0 | 0 | 2 | 1 | 9 | 5 | 1 | 1 | 7 | 1 | 2 | 0 | 0 | 5 | 3 | 2 | 3 |
Endosome | 2 | 1 | 5 | 0 | 4 | 11 | – | 16 | 5 | 7 | 4 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 2 | 5 | 1 | 2 | 2 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 1 | 0 | 1 | 2 |
Mitochondria | 2 | 3 | 6 | 15 | 40 | 145 | – | 247 | 271 | 389 | 434 | 489 | 0 | 0 | 0 | 5 | 5 | 1 | 1 | 1 | 5 | 6 | 7 | 8 |
Nucleus | 0 | 2 | 0 | 2 | 2 | 2 | – | 10 | 3 | 7 | 9 | 7 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | – | 3 | 1 | 7 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | – | 2 | 1 | 5 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 5 | 1 | 2 | 7 |
Vac/Vac Membrane | 5 | 0 | 8 | 3 | 15 | 31 | – | 66 | 40 | 66 | 43 | 46 | 2 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 1 | 4 | 2 | 4 |
Unique Cell Count | 358 | 233 | 614 | 369 | 566 | 647 | 877 | 596 | 765 | 739 | 869 | 223 | 274 | 286 | 144 | 152 | 75 | 364 | 234 | 595 | 375 | 406 | 401 | |
Labelled Cell Count | 366 | 236 | 630 | 387 | 605 | 763 | 1036 | 762 | 988 | 954 | 1086 | 231 | 276 | 293 | 155 | 157 | 80 | 364 | 234 | 595 | 375 | 406 | 401 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.9 | 5.4 | 5.3 | 4.9 | 4.3 | – | 4.4 | 4.1 | 3.8 | 3.8 | 3.9 | 6.2 | 6.3 | 6.1 | 5.2 | 6.0 | 7.3 | 6.4 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.3 | 1.5 | 1.8 | 1.6 | 1.3 | – | 1.4 | 1.0 | 0.8 | 0.9 | 0.9 | 0.8 | 0.8 | 0.7 | 1.1 | 1.2 | 1.5 | 1.2 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.13 | -0.32 | – | -0.27 | -0.4 | -0.51 | -0.48 | -0.48 | 0.21 | 0.22 | 0.19 | -0.05 | 0.15 | 0.45 | 0.25 | 0.34 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | -2.2 | -6.9 | – | -11.0 | -12.9 | -15.4 | -17.3 | -17.4 | -0.6 | 1.7 | 1.8 | -0.2 | 0.3 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 5.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.7 | 3.6 | – | 5.4 | 4.8 | 6.0 | 4.3 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.844 | 1.2597 | 1.0515 | 0.8109 | 0.1912 | 1.1401 | 2.584 | 3.249 | 3.0825 | 2.287 | 2.7681 | 3.2894 | 1.9386 | 3.1847 | 2.5465 | 3.3388 | 2.3053 | 2.9712 |
Actin | 0.021 | 0.0003 | 0.0084 | 0.0171 | 0.0003 | 0.0034 | 0.0409 | 0.003 | 0.0062 | 0.0112 | 0.0015 | 0.0018 | 0.0005 | 0.0049 | 0.0012 | 0.0035 | 0.0001 | 0.0002 |
Bud | 0.0002 | 0.0004 | 0.0005 | 0.0031 | 0.0001 | 0.0018 | 0.0054 | 0.0006 | 0.0054 | 0.0004 | 0.0013 | 0.0003 | 0.0001 | 0.0027 | 0.0031 | 0.0002 | 0 | 0 |
Bud Neck | 0.0015 | 0.0002 | 0.0008 | 0.0146 | 0.0001 | 0.001 | 0.0021 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0083 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Bud Periphery | 0.0003 | 0.0002 | 0.0005 | 0.0099 | 0 | 0.0011 | 0.0011 | 0.0007 | 0.0055 | 0.0005 | 0.0009 | 0.0004 | 0.0001 | 0.0029 | 0.002 | 0.0002 | 0 | 0 |
Bud Site | 0.0006 | 0.0039 | 0.0013 | 0.0019 | 0 | 0.0002 | 0.0178 | 0.0012 | 0.0031 | 0.0004 | 0.0004 | 0.0001 | 0.001 | 0.0062 | 0.0137 | 0.0032 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0 | 0 |
Cytoplasm | 0.4442 | 0.6306 | 0.5871 | 0.5957 | 0.6309 | 0.6334 | 0.2752 | 0.4689 | 0.4037 | 0.5164 | 0.3683 | 0.4357 | 0.3765 | 0.3741 | 0.4168 | 0.4444 | 0.6025 | 0.5041 |
Cytoplasmic Foci | 0.0204 | 0.0082 | 0.0094 | 0.013 | 0.0115 | 0.0074 | 0.0122 | 0.0052 | 0.0089 | 0.0101 | 0.0102 | 0.0065 | 0.0065 | 0.0073 | 0.006 | 0.0111 | 0.006 | 0.0078 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0057 | 0.002 | 0.0026 | 0.0051 | 0.0019 | 0.002 | 0.0028 | 0.002 | 0.0018 | 0.01 | 0.0011 | 0.0013 | 0.0051 | 0.0028 | 0.0019 | 0.0028 | 0.0019 | 0.0019 |
Endosome | 0.0086 | 0.0012 | 0.004 | 0.0088 | 0.003 | 0.0062 | 0.0061 | 0.0026 | 0.0012 | 0.0042 | 0.0007 | 0.0012 | 0.0006 | 0.0005 | 0.0008 | 0.0325 | 0.0022 | 0.001 |
Golgi | 0.0039 | 0.0001 | 0.0021 | 0.0034 | 0.0002 | 0.0002 | 0.0033 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0136 | 0.0001 | 0.0001 |
Lipid Particles | 0.0036 | 0.0003 | 0.0025 | 0.0009 | 0.0005 | 0.0003 | 0.0063 | 0.0003 | 0.0018 | 0.0006 | 0.0004 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.011 | 0.0001 | 0.0001 |
Mitochondria | 0.0017 | 0.0002 | 0.0013 | 0.0287 | 0.0004 | 0.0004 | 0.0016 | 0.0002 | 0.0052 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0045 | 0.0001 | 0.0001 |
None | 0.4776 | 0.3487 | 0.3687 | 0.2833 | 0.3464 | 0.3339 | 0.5868 | 0.511 | 0.5452 | 0.4363 | 0.6054 | 0.5412 | 0.6047 | 0.593 | 0.5499 | 0.4649 | 0.3841 | 0.4819 |
Nuclear Periphery | 0.0024 | 0.0005 | 0.0011 | 0.0021 | 0.0006 | 0.0037 | 0.0017 | 0.0007 | 0.0007 | 0.0026 | 0.0014 | 0.0003 | 0.0007 | 0.0006 | 0.0005 | 0.0011 | 0.0005 | 0.0004 |
Nucleolus | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 |
Nucleus | 0.0013 | 0.0012 | 0.0022 | 0.0046 | 0.0018 | 0.0023 | 0.0024 | 0.0013 | 0.0022 | 0.0021 | 0.0016 | 0.001 | 0.0009 | 0.0007 | 0.0008 | 0.0008 | 0.001 | 0.0005 |
Peroxisomes | 0.0013 | 0.0002 | 0.004 | 0.0013 | 0.0007 | 0.0002 | 0.0271 | 0.0002 | 0.0049 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0025 | 0 | 0.0002 |
Punctate Nuclear | 0.0041 | 0.0009 | 0.002 | 0.0019 | 0.0007 | 0.0013 | 0.0017 | 0.0006 | 0.0017 | 0.0012 | 0.0047 | 0.0007 | 0.0012 | 0.0012 | 0.0011 | 0.001 | 0.0002 | 0.0014 |
Vacuole | 0.0007 | 0.0007 | 0.0009 | 0.0035 | 0.0006 | 0.0007 | 0.0029 | 0.0006 | 0.0005 | 0.0011 | 0.0004 | 0.0003 | 0.0006 | 0.0005 | 0.0005 | 0.0014 | 0.001 | 0.0002 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.0172 | 3.5064 | 3.8028 | 2.8598 | 4.1076 | 3.3171 | 3.6817 | 4.0494 | 4.0354 | 4.9086 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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