Standard name
Human Ortholog
Description Histone acetyltransferase; critical for cell survival in presence of DNA damage during S phase, required for recovery after DSB repair; acetylates H3K56, H3K9; H3K56 acetylation activity required for expression homeostasis, buffering of mRNA synthesis rate against changes in gene dosage during S phase; involved in non-homologous end joining and regulation of Ty1 transposition; prevents hyper-amplification of rDNA; interacts physically with Vps75p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.06 0.07 0.07 0.09 0 0.05 0 0 0 0 0 0.18 0.24 0.26 0.18 0.14 0.17 0 0 0 0.07 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.24 0.1 0.18 0.27 0.51 0.59 0.43 0.43 0.58 0.66 0.54 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0.76 0.82 0.82 0.81 0.74 0.75 0.69 0.76 0.75 0.76 0.71 0.73 0.78 0.73 0.7 0.79 0.77 0.76 0.7 0.79 0.74 0.6 0.53 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.05 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0.07 0.18 0.1 0.15 0.24 0.24 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 2
Bud 1 0 2 1 0 13 12 5 7 18 9 11 0 0 0 0 0 1 1 5 5 2 9 12
Bud Neck 0 0 1 0 0 0 1 0 0 1 0 0 2 1 1 0 0 0 0 0 0 2 3 4
Bud Site 1 0 1 1 6 13 11 22 18 25 19 25 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 14 23 39 25 46 33 42 28 27 18 21 10 99 118 160 68 54 70 5 15 16 31 35 46
Endoplasmic Reticulum 0 2 1 0 1 0 0 0 0 0 0 0 1 2 5 14 12 20 1 0 0 2 3 3
Endosome 0 0 18 8 11 0 0 4 1 3 0 0 2 3 7 3 9 8 2 4 4 3 6 11
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1 0 3 1 0 1 3
Mitochondria 64 94 57 68 131 406 498 311 350 474 482 382 2 0 3 9 13 15 4 3 10 12 22 31
Nucleus 122 326 459 302 364 593 587 555 605 622 512 518 430 353 439 291 300 316 135 331 474 264 293 281
Nuclear Periphery 3 0 1 2 3 3 2 5 8 7 8 8 0 0 1 0 0 0 1 0 1 0 1 5
Nucleolus 0 0 0 1 2 4 12 19 14 21 23 27 0 0 0 1 1 2 0 0 2 2 6 11
Peroxisomes 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
SpindlePole 2 1 3 1 2 0 2 1 4 2 0 3 3 3 3 1 5 1 3 7 15 8 18 28
Vac/Vac Membrane 10 28 28 14 25 27 27 15 24 15 17 36 20 18 19 10 31 30 34 43 94 105 135 226
Unique Cell Count 160 397 561 371 491 794 849 730 809 818 726 706 549 485 625 370 392 414 194 422 640 442 554 687
Labelled Cell Count 217 474 613 423 591 1092 1194 965 1059 1206 1091 1020 559 498 641 399 429 464 194 422 640 442 554 687


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.5 4.6 4.4 4.5 3.8 3.9 4.0 3.9 3.8 3.4 3.6 6.0 5.9 5.7 7.1 6.8 7.0 5.9 6.1 6.2
Std Deviation (1e-4) 0.4 0.7 0.9 1.6 2.0 1.2 1.6 1.0 0.8 0.8 0.8 0.8 1.1 0.9 1.1 1.6 1.7 1.9 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.07 -0.01 -0.28 -0.22 -0.21 -0.24 -0.29 -0.41 -0.34 0.39 0.36 0.32 0.63 0.57 0.61 0.37 0.41 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.7 1.8 1.5