Standard name
Human Ortholog
Description Plasma membrane-associated protein phosphatase; involved in the general stress response and the inactivating dephosphorylation of Mep2p; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr2p; member of the HAD family of protein phosphatases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.38 0.25 0.25 0.31 0.29 0.16 0.15 – 0.14 0.22 0.19 0.13 0.19 0.11 0.07 0.09 0 0 0 0 0 0 0
Bud – 0 0 0 0 0.05 0 0 – 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0.88 0.86 0.81 0.65 0.63 0.51 0.38 – 0.39 0.48 0.48 0.91 0.84 0.84 0.71 0.65 0.66 0.85 0.92 0.93 0.91 0.92 0.91
Cytoplasm – 0.1 0.12 0.15 0.22 0.24 0.32 0.37 – 0.26 0.28 0.29 0.16 0.21 0.2 0.28 0.3 0.29 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.3 0.36 0.5 0.42 0.47 0.47 0.46 – 0.61 0.6 0.6 0 0 0 0.1 0.08 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 46 82 78 150 105 89 62 – 82 78 92 39 71 37 4 12 1 0 2 5 1 6 6
Bud – 4 7 12 21 19 19 19 – 29 13 25 11 10 6 1 4 1 0 0 0 0 2 0
Bud Neck – 0 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 2 2 4 2 4 3
Bud Site – 0 0 0 0 0 1 1 – 1 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery – 106 287 255 320 224 278 160 – 229 169 239 278 320 269 41 82 25 62 121 355 178 474 565
Cytoplasm – 12 39 46 109 85 178 157 – 154 100 142 48 80 64 16 38 11 0 0 2 2 8 12
Endoplasmic Reticulum – 2 1 0 8 8 6 7 – 6 1 2 4 6 8 0 7 4 0 2 4 2 7 16
Endosome – 0 0 0 0 0 0 3 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Mitochondria – 36 119 157 206 169 256 195 – 363 213 295 0 8 3 6 10 1 2 0 4 6 6 7
Nucleus – 0 1 0 0 0 1 3 – 6 1 1 4 2 6 1 3 1 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 2 5 – 5 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 2 11 10 13 7 – 4 1 6 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole – 0 1 0 5 0 2 2 – 0 0 0 2 8 6 1 3 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 2 0 6 5 – 6 0 2 1 0 0 0 3 0 1 0 0 0 3 1
Unique Cell Count 121 333 316 490 357 550 425 593 354 493 307 382 322 58 127 38 73 131 382 196 517 621
Labelled Cell Count 206 537 550 834 621 851 627 885 576 806 387 506 399 70 163 44 73 131 382 196 517 621


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 8.6 7.1 7.3 6.5 6.1 6.1 6.1 – 5.7 5.9 6.1 8.8 8.5 9.1 6.4 8.1 9.2 9.2 9.5 9.6
Std Deviation (1e-4) – 0.9 0.9 1.1 1.1 0.9 1.3 0.9 – 0.9 1.2 1.0 0.9 0.9 0.9 1.2 1.7 2.2 1.1 1.3 1.3
Intensity Change (Log2) – – – 0.06 -0.11 -0.2 -0.21 -0.2 – -0.31 -0.25 -0.22 0.33 0.27 0.37 -0.15 0.2 0.39 0.39 0.42 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.9 1.4 -3.1 -3.5 – -4.1 -0.8 -2.1 -3.8 -2.0 -4.4 -3.0 -3.6 -3.1
Bud 1.3 1.7 2.2 1.2 1.8 – 2.1 1.2 2.2 1.1 0.5 -0.2 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 -6.7 -7.0 -10.7 -13.5 – -14.0 -10.7 -11.1 1.7 -0.9 -0.9 -3.0 -5.2 -3.3
Cytoplasm 1.1 3.9 4.1 6.9 7.9 – 5.1 5.4 5.8 1.4 3.3 2.9 3.2 4.7 2.9
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 1.8 3.1 3.1 2.8 – 7.4 6.4 6.8 -11.6 -11.7 -11.4 -3.8 -5.9 -4.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3411 5.7441 5.5544 5.3209 4.9574 5.356 4.7291 6.2333 5.2166 4.8739 4.7 5.236 4.2463 6.0076 4.8283 5.5172 4.6325 5.2325
Actin 0.0095 0.0002 0.0005 0.0012 0.0635 0.0003 0.034 0 0.0109 0.0075 0 0.0004 0.0019 0 0.0021 0.0005 0.012 0.0016
Bud 0.0003 0.0031 0.0006 0.0218 0.0223 0.0012 0.001 0 0.0004 0.0001 0 0.0015 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003
Bud Neck 0.0005 0.0012 0.0015 0.0009 0.0039 0.0028 0.0092 0.0003 0.0042 0.0005 0.0001 0.001 0.0004 0.0015 0.0091 0.0128 0.0007 0.001
Bud Periphery 0.0059 0.0037 0.0078 0.0359 0.0317 0.0068 0.0062 0.0005 0.0042 0.0015 0.0016 0.0044 0.0005 0.001 0.002 0.0029 0.007 0.0033
Bud Site 0.0015 0.0217 0.0122 0.0073 0.0328 0.0021 0.0063 0.0042 0.0224 0.0004 0.0001 0.0008 0.0013 0.0172 0.0152 0.0016 0.0007 0.0005
Cell Periphery 0.8835 0.851 0.8683 0.8505 0.751 0.9189 0.8619 0.9345 0.8881 0.8488 0.9738 0.9445 0.8524 0.9073 0.881 0.9472 0.9152 0.9545
Cytoplasm 0.0542 0.105 0.0809 0.0687 0.0357 0.0559 0.0309 0.0562 0.0331 0.0188 0.0101 0.0177 0.0764 0.0664 0.0651 0.0278 0.0105 0.019
Cytoplasmic Foci 0.0021 0.0001 0.0001 0.0002 0.003 0.0001 0.0102 0 0.0013 0.0398 0 0.0021 0.0206 0 0.0025 0.0001 0.0027 0.0012
Eisosomes 0.0039 0.0036 0.0021 0.0011 0.0012 0.0004 0.0031 0.0003 0.003 0.0002 0.0057 0.0042 0.0029 0.0012 0.0003 0.0017 0.0007 0.0002
Endoplasmic Reticulum 0.0084 0.0045 0.0203 0.0042 0.015 0.0044 0.0043 0.0022 0.0018 0.0044 0.0054 0.0025 0.0042 0.0017 0.0032 0.0023 0.0059 0.0045
Endosome 0.0021 0 0 0 0.0086 0 0.0031 0 0.0016 0.0162 0 0.0016 0.0023 0 0.0048 0 0.0027 0.0024
Golgi 0.0021 0.0001 0.0001 0.0001 0.0048 0.0001 0.004 0 0.0025 0.0137 0 0.0008 0.0015 0 0.0029 0 0.003 0.0013
Lipid Particles 0.0055 0.0003 0.0002 0.0003 0.0034 0.0005 0.0029 0.0001 0.0054 0.0271 0.0001 0.0053 0.0059 0.0001 0.0057 0.0002 0.0163 0.0041
Mitochondria 0.0029 0 0.0001 0.0002 0.0024 0.0001 0.0044 0 0.0067 0.002 0.0003 0.0005 0.0002 0 0.0002 0 0.0005 0.0002
None 0.0027 0.0023 0.0006 0.0009 0.0056 0.0003 0.0008 0.0001 0.0008 0.0009 0.0002 0.008 0.0013 0.0001 0.0002 0.0002 0.002 0.0002
Nuclear Periphery 0.0017 0.0003 0.0003 0.0002 0.0032 0.0001 0.0013 0.0002 0.0002 0.0004 0.0002 0.0001 0.0007 0.0003 0.0003 0.0002 0.0018 0.0005
Nucleolus 0.0001 0 0.0001 0.0001 0.0009 0.0001 0.0004 0 0.0001 0.0001 0 0.0003 0.0001 0 0.0001 0 0.0002 0
Nucleus 0.0003 0.0001 0.0001 0.0003 0.0025 0.0003 0.001 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001
Peroxisomes 0.0065 0 0 0 0.001 0 0.0063 0 0.0098 0.0127 0 0.0006 0.0118 0 0.0007 0 0.0091 0.0013
Punctate Nuclear 0.0004 0 0 0 0.0016 0 0.0021 0 0.0001 0.0017 0 0.0002 0.011 0 0.0002 0 0.003 0.0005
Vacuole 0.0055 0.0029 0.0041 0.0058 0.0055 0.0054 0.0062 0.0013 0.0031 0.003 0.0024 0.0031 0.0045 0.0027 0.0042 0.0022 0.0054 0.0033
Vacuole Periphery 0.0004 0 0.0001 0.0001 0.0004 0 0.0003 0 0.0003 0.0002 0 0.0001 0 0 0.0001 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.4837 26.8872 17.1013 25.8637 19.4982 17.1775 27.574 24.3772 25.1758 26.4529
Translational Efficiency 1.4887 1.3055 1.6774 1.4217 1.713 1.6592 1.243 1.3479 1.3706 1.2278

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane-associated protein phosphatase; involved in the general stress response and the inactivating dephosphorylation of Mep2p; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr2p; member of the HAD family of protein phosphatases
Localization
Cell Percentages cell periphery (89%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Psr1

Psr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available