Standard name
Human Ortholog
Description Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.57 0.55 0.52 0.81 0.8 0.87 – 0.88 0.86 0.86 0.82 0.84 0.4 0.58 0.72 – – 0.77 0.06 0.07 0 0.05 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.05 0 0 0.05 0 0 0.05
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.11 0 0.05 0 0.1 – 0.13 0.24 0.26 0.38 0.28 0 0 0 – – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0.19 0.15 0.06 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.45 0.6 0.24 0.31 0.16 – 0.07 0.05 0.05 0.06 0.06 0.47 0.36 0.32 – – 0.23 0.84 0.83 0.84 0.85 0.84 0.78
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 1 0 0 0 0 0 1 0 – – 0 0 1 0 0 0 1
Bud 0 1 0 0 0 5 – 9 13 15 11 9 1 0 0 – – 0 0 3 0 3 1 5
Bud Neck 0 1 0 0 0 0 – 0 1 0 0 0 0 0 0 – – 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 147 184 108 142 240 335 – 484 429 515 332 437 21 67 145 – – 117 16 29 6 5 16 19
Endoplasmic Reticulum 1 7 1 0 3 0 – 0 1 0 1 2 3 0 2 – – 2 1 1 3 1 7 2
Endosome 6 0 16 6 11 7 – 9 5 14 2 4 0 0 0 – – 7 11 11 10 3 11 21
Golgi 1 1 1 0 0 0 – 0 0 0 0 0 0 0 0 – – 1 1 0 1 0 0 1
Mitochondria 8 38 8 9 5 40 – 69 118 154 155 143 2 0 0 – – 4 4 8 6 2 9 16
Nucleus 5 11 1 1 3 0 – 3 3 1 3 1 10 17 12 – – 3 2 4 0 1 1 4
Nuclear Periphery 0 5 0 0 0 0 – 0 0 1 1 2 0 0 0 – – 0 1 1 2 0 1 3
Nucleolus 0 0 1 0 0 0 – 0 0 1 0 2 0 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 0 0 1 0 0 0 – 2 0 1 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 – 2 2 2 2 4 0 0 0 – – 0 1 1 0 0 1 4
Vac/Vac Membrane 152 149 125 42 92 60 – 36 25 30 24 32 25 41 65 – – 35 237 326 187 109 298 323
Unique Cell Count 259 333 208 176 299 385 548 501 599 405 519 53 115 201 152 283 394 222 129 356 413
Labelled Cell Count 320 398 263 200 354 447 615 597 734 532 638 62 126 224 169 283 394 222 129 356 413


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.4 4.7 4.8 4.8 3.8 – 3.9 3.6 3.4 3.5 3.5 5.4 5.6 5.8 4.2 4.7 5.8 6.2 6.2 6.5
Std Deviation (1e-4) 1.2 0.8 1.4 1.7 1.8 1.5 – 1.0 1.1 1.1 1.3 0.9 1.0 1.0 1.2 1.3 1.1 1.6 1.9 1.7 1.8
Intensity Change (Log2) – – – 0.03 0.02 -0.3 – -0.27 -0.38 -0.48 -0.42 -0.44 0.19 0.24 0.3 -0.16 0.0 0.28 0.38 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – -7.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7928 1.2242 0.9207 0.9441 1.1143 0.8974 2.2509 2.8474 2.8135 3.1079 1.9687 2.6032 2.4854 2.7865 2.8326 2.4587 2.357 2.964
Actin 0.0766 0.0174 0.0033 0.0038 0.0233 0.0002 0.0447 0 0.0208 0.0131 0.0031 0.0012 0.0596 0.0009 0.0054 0 0.0706 0.0005
Bud 0.0007 0.0009 0.0007 0.0008 0.0002 0.0004 0.0008 0.0022 0.0006 0.0011 0.001 0.0007 0.0008 0.0014 0.0011 0.0005 0.0014 0.0006
Bud Neck 0.0028 0.0006 0.0014 0.0006 0.0002 0.0005 0.0021 0.0003 0.0008 0.0008 0.0007 0.0014 0.0011 0.0011 0.0005 0.0004 0.0009 0.0021
Bud Periphery 0.001 0.0009 0.0007 0.0007 0.0003 0.0005 0.0013 0.0011 0.0008 0.0011 0.0009 0.0009 0.0017 0.0014 0.0007 0.0006 0.0022 0.0006
Bud Site 0.0198 0.0058 0.0104 0.0015 0.0009 0.0005 0.0058 0.0024 0.0026 0.002 0.0019 0.0016 0.0033 0.0097 0.0015 0.0012 0.0015 0.0009
Cell Periphery 0.0021 0.0006 0.0004 0.0002 0.0001 0.0003 0.0008 0.0003 0.0005 0.0003 0.0002 0.0004 0.0007 0.0008 0.0005 0.0003 0.0002 0.0004
Cytoplasm 0.0595 0.1255 0.1094 0.0192 0.0466 0.0742 0.187 0.3145 0.2017 0.184 0.1249 0.266 0.1113 0.1575 0.1238 0.0638 0.0847 0.1236
Cytoplasmic Foci 0.0579 0.03 0.0464 0.076 0.0789 0.0236 0.0698 0.0383 0.043 0.0342 0.0847 0.0426 0.0419 0.017 0.035 0.02 0.0183 0.0374
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0007 0 0.0002 0.0001 0.0001 0 0.0004 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0038 0.0051 0.0179 0.0081 0.0017 0.0064 0.0054 0.0043 0.0057 0.0013 0.0019 0.0037 0.0103 0.0072 0.0064 0.0038 0.004 0.007
Endosome 0.2936 0.2074 0.3068 0.328 0.3371 0.2423 0.1901 0.1344 0.1949 0.1807 0.2673 0.2041 0.2142 0.1374 0.2364 0.2396 0.2738 0.242
Golgi 0.0182 0.0024 0.0065 0.0217 0.0057 0.0019 0.0117 0.0013 0.0046 0.0296 0.0091 0.0025 0.0066 0.0019 0.0037 0.0018 0.0086 0.0029
Lipid Particles 0.0081 0.0093 0.0141 0.0219 0.0086 0.0048 0.0164 0.0013 0.0085 0.0037 0.006 0.0021 0.0143 0.0031 0.0104 0.0008 0.0052 0.0062
Mitochondria 0.0061 0.0015 0.0112 0.0083 0.0015 0.002 0.0074 0.0012 0.018 0.053 0.0185 0.0067 0.0105 0.0028 0.0048 0.0032 0.0035 0.0107
None 0.009 0.0622 0.0756 0.0011 0.0013 0.1265 0.2373 0.2149 0.2384 0.2011 0.1275 0.185 0.1166 0.093 0.0915 0.0694 0.0909 0.0599
Nuclear Periphery 0.0033 0.0069 0.0219 0.0053 0.0028 0.0055 0.0071 0.004 0.0042 0.0015 0.0016 0.0027 0.0196 0.0096 0.0091 0.0098 0.0067 0.0174
Nucleolus 0.0009 0.0029 0.0014 0.0013 0.0003 0.0008 0.0016 0.001 0.0013 0.0005 0.0009 0.0005 0.0013 0.0018 0.0019 0.0008 0.0005 0.0014
Nucleus 0.0028 0.0172 0.006 0.0017 0.0022 0.0049 0.0033 0.0073 0.0056 0.0024 0.0026 0.0039 0.0068 0.0077 0.0068 0.0029 0.0031 0.0089
Peroxisomes 0.0121 0.0005 0.0063 0.0051 0.0461 0.0137 0.0145 0.0002 0.0061 0.003 0.0133 0.0012 0.0112 0.0008 0.0012 0.0002 0.0025 0.001
Punctate Nuclear 0.0217 0.0006 0.0025 0.0013 0.0015 0.0007 0.0043 0.002 0.0008 0.0002 0.0237 0.0006 0.0085 0.0006 0.0016 0.0002 0.0016 0.0026
Vacuole 0.35 0.451 0.2973 0.4068 0.3489 0.3962 0.1754 0.2427 0.2112 0.2535 0.2758 0.2321 0.3154 0.4609 0.389 0.4804 0.3625 0.4072
Vacuole Periphery 0.0496 0.0512 0.0598 0.0866 0.0917 0.094 0.0125 0.0261 0.0298 0.0326 0.0343 0.0401 0.044 0.0832 0.0687 0.1004 0.0569 0.0669

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.7565 33.6631 23.2686 30.2399 14.3797 32.4806 52.3374 42.0114 37.3707 49.6122
Translational Efficiency 0.387 0.4404 0.5711 0.3245 0.4341 0.5012 0.3744 0.4695 0.4884 0.3717

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
188 1087 1776 1314 2151 1293 1820 1638 2339 2380 3596 2952

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 900.47 746.73 830.87 891.72 831.49 752.09 863.18 879.60 837.03 749.64 847.22 884.99
Standard Deviation 158.24 120.59 96.08 119.10 107.24 95.01 95.68 113.58 113.76 107.48 97.23 116.23
Intensity Change Log 2 — -0.270092 -0.116055 -0.014087 — -0.144794 0.053963 0.081149 — -0.208578 -0.031929 0.032419

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001675 0.000067 0.000123 0.000396 0.000006 0.000076 0.000050 0.000530 0.000140 0.000072 0.000086 0.000470
Bud Neck 0.007963 0.009973 0.007612 0.014464 0.002256 0.013663 0.016086 0.017670 0.002715 0.011978 0.011901 0.016243
Bud Site 0.048970 0.016973 0.008954 0.022167 0.002115 0.011491 0.006334 0.025874 0.005881 0.013995 0.007628 0.024224
Cell Periphery 0.000236 0.000112 0.000060 0.000082 0.000087 0.000162 0.000033 0.000064 0.000099 0.000139 0.000046 0.000072
Cytoplasm 0.202164* 0.128349* 0.526250* 0.187394* 0.013438 0.090819 0.293427* 0.349759* 0.028607 0.107960* 0.408414* 0.277487*
Cytoplasmic Foci 0.074238 0.081357 0.027341 0.037867 0.007733 0.076686 0.023774 0.037326 0.013079 0.078819 0.025536 0.037567
Eisosomes 0.000092 0.000032 0.000019 0.000017 0.000004 0.000045 0.000015 0.000022 0.000011 0.000039 0.000017 0.000019
Endoplasmic Reticulum 0.002897 0.003693 0.002829 0.003426 0.003302 0.008790 0.002658 0.001609 0.003269 0.006462 0.002742 0.002418
Endosome 0.045139 0.079261 0.011296 0.046381 0.025933 0.090801 0.030478 0.034495 0.027477 0.085530 0.021004 0.039786
Golgi 0.022028 0.002128 0.000351 0.004045 0.001037 0.004507 0.000227 0.003824 0.002724 0.003420 0.000288 0.003922
Lipid Particles 0.003667 0.003481 0.001478 0.001805 0.000580 0.008449 0.001434 0.001611 0.000828 0.006180 0.001456 0.001698
Mitochondria 0.008016 0.005545 0.000881 0.002400 0.002437 0.013017 0.000949 0.002589 0.002885 0.009604 0.000915 0.002505
Mitotic Spindle 0.022371 0.001315 0.004654 0.012386 0.000157 0.002289 0.003611 0.019190 0.001943 0.001844 0.004126 0.016161
None 0.000491 0.001722 0.003457 0.000470 0.000093 0.001448 0.001033 0.000906 0.000125 0.001573 0.002230 0.000712
Nuclear Periphery 0.001930 0.000597 0.002923 0.001957 0.000278 0.001693 0.002968 0.001595 0.000411 0.001192 0.002946 0.001756
Nuclear Periphery Foci 0.002759 0.000364 0.001760 0.001405 0.000066 0.000339 0.001017 0.001169 0.000282 0.000351 0.001384 0.001274
Nucleolus 0.000700 0.001700 0.002095 0.001617 0.000290 0.001720 0.004383 0.001701 0.000323 0.001710 0.003253 0.001663
Nucleus 0.065270 0.021728 0.255724* 0.031060 0.003026 0.030553 0.127844 0.060564 0.008029 0.026522 0.191002* 0.047431
Peroxisomes 0.003131 0.002654 0.000737 0.001794 0.000191 0.002670 0.000634 0.001412 0.000427 0.002663 0.000685 0.001582
Vacuole 0.473910* 0.536941* 0.137542 0.612942* 0.769752* 0.584685* 0.472337* 0.429407* 0.745974* 0.562880* 0.306988* 0.511103*
Vacuole Periphery 0.012353 0.102008* 0.003913 0.015925 0.167217* 0.056097 0.010709 0.008684 0.154770* 0.077066 0.007352 0.011907

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.75 2.61 2.14 -3.48 -2.64 -5.29 -13.45 -2.65 -2.20 -2.29 1.10 0.46 -2.68 -3.35 -3.08
Bud Neck -2.11 -0.86 -4.23 -3.28 -5.29 -8.13 -13.52 -12.06 -1.33 0.77 -10.20 -13.04 -14.70 -2.94 -2.92
Bud Site 2.07 3.01 1.79 -1.10 -5.43 -6.66 -4.38 -9.80 -3.39 -7.11 -7.09 -2.03 -10.03 -3.28 -8.77
Cell Periphery 2.38 3.87 3.28 2.56 -1.18 -5.73 8.12 4.54 9.03 -3.84 -4.46 7.10 3.82 8.33 -2.47
Cytoplasm 3.88 -16.44 -0.91 -9.68 30.42 -16.50 -45.50 -47.52 -31.51 -5.13 -18.77 -70.96* -48.08 -29.37 15.68
Cytoplasmic Foci -3.14 5.54 4.55 12.18 -2.80 -19.65 -12.66 -15.51 12.22 -5.71 -25.15 -10.79 -15.14 17.25 -5.77
Eisosomes 8.09 10.05 10.65 5.73 2.96 -16.66 -13.68 -10.11 8.18 -2.80 -14.47 -7.03 -6.44 9.90 -0.70
Endoplasmic Reticulum -1.12 0.07 0.39 1.44 0.44 -6.81 2.55 7.55 9.29 7.63 -6.43 1.92 3.95 8.65 3.16
Endosome -8.21 4.81 -0.24 12.25 -12.10 -17.95 0.34 1.25 18.15 0.91 -22.35 5.85 -1.13 21.82 -7.37
Golgi 2.29 2.64 2.17 -0.66 -3.07 -3.90 1.95 -1.54 2.17 -3.05 -1.80 3.14 -0.44 1.46 -4.31
Lipid Particles -0.11 3.52 2.81 4.53 -2.20 -10.49 -5.12 -5.78 9.31 -1.52 -11.85 -4.15 -5.52 10.27 -2.57
Mitochondria -0.04 2.11 1.64 4.22 -1.86 -7.01 2.54 0.84 7.74 -2.25 -6.86 3.12 1.34 8.78 -2.92
Mitotic Spindle 2.48 1.95 0.49 -5.43 -3.81 -3.14 -4.61 -8.06 -6.73 -5.73 -0.58 -3.54 -8.81 -8.66 -6.84
None -2.76 -8.80 -0.54 2.62 8.63 -2.62 -11.71 -12.26 0.94 1.00 -4.14 -12.76 -11.04 2.31 8.31
Nuclear Periphery 2.73 -2.39 -0.63 -4.02 1.78 -5.20 -16.59 -11.91 -1.13 6.59 -4.67 -20.32 -8.07 -3.88 4.85
Nuclear Periphery Foci 4.44 0.72 1.92 -6.82 1.56 -3.20 -11.10 -10.06 -5.98 0.49 -1.18 -7.91 -10.11 -9.07 1.76
Nucleolus -3.06 -5.27 -4.89 0.29 2.10 -5.69 -11.89 -9.41 0.01 7.55 -6.92 -13.69 -12.76 0.20 7.30
Nucleus 5.18 -15.82 3.19 -7.00 37.88 -11.35 -35.54 -27.10 -12.96 13.84 -8.98 -53.10 -25.73 -14.58 35.92
Peroxisomes -0.54 2.52 1.33 2.22 -3.10 -9.50 -8.35 -8.41 5.81 -4.53 -7.94 -3.59 -7.19 4.80 -4.82
Vacuole -0.40 15.23 -2.81 -5.18 -48.09* 19.26 38.77* 38.47* 12.73 1.22 24.42 66.02* 33.08 6.30 -27.58
Vacuole Periphery -16.00 1.37 -0.75 18.98 -7.39 24.95* 41.51* 42.17* 20.65 3.45 17.69 41.01 40.23 26.57 -2.96
Standard name
Human Ortholog
Description Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
Localization
Cell Percentages vacuole (33%), vacuolar membrane (24%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fre6

Fre6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fre6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available