ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 2 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 6 | 10 | 9 |
Bud | 0 | 0 | 1 | 0 | 1 | 0 | 3 | – | 2 | 7 | 9 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 12 | 14 | 12 |
Bud Neck | 0 | 1 | 4 | 5 | 13 | 12 | 12 | – | 10 | 5 | 4 | 5 | 0 | 0 | 0 | 7 | 2 | 0 | 0 | 1 | 0 | 2 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 9 | 6 | 3 | 2 | 1 | – | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 8 | 9 | 6 | 0 | 1 | 3 | 3 | 3 | 7 |
Cytoplasm | 120 | 348 | 319 | 236 | 291 | 358 | 443 | – | 347 | 291 | 285 | 283 | 520 | 498 | 665 | 537 | 340 | 397 | 118 | 326 | 271 | 266 | 262 | 285 |
Endoplasmic Reticulum | 1 | 2 | 0 | 1 | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 45 | 14 | 33 | 0 | 0 | 0 | 3 | 3 | 4 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 3 | 3 | 1 | 5 | 3 | 3 | 5 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 2 |
Mitochondria | 2 | 7 | 0 | 0 | 0 | 23 | 49 | – | 108 | 128 | 140 | 106 | 7 | 2 | 6 | 12 | 3 | 1 | 3 | 15 | 26 | 56 | 53 | 55 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 6 | – | 4 | 4 | 9 | 6 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 1 | 7 | 6 | 9 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 2 | 3 | 3 | 0 | 0 | 1 | 2 | 6 | 8 | 3 | 1 | 5 | 2 | 17 | 22 | 27 |
Unique Cell Count | 123 | 356 | 321 | 238 | 298 | 374 | 473 | 396 | 362 | 379 | 345 | 522 | 503 | 674 | 562 | 350 | 404 | 129 | 365 | 317 | 395 | 397 | 440 | |
Labelled Cell Count | 123 | 359 | 334 | 250 | 315 | 400 | 518 | 478 | 441 | 457 | 407 | 530 | 505 | 677 | 627 | 383 | 448 | 129 | 365 | 317 | 395 | 397 | 440 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.2 | 4.6 | 4.3 | 4.9 | 3.6 | 3.8 | – | 3.2 | 3.1 | 3.0 | 3.5 | 3.9 | 3.9 | 4.2 | 5.7 | 6.0 | 5.6 | 3.8 | 3.9 | 4.3 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.7 | 0.8 | 1.5 | 0.8 | 0.9 | – | 0.6 | 0.6 | 0.5 | 0.9 | 1.0 | 1.0 | 1.5 | 1.2 | 1.5 | 1.2 | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.07 | -0.37 | -0.28 | – | -0.55 | -0.56 | -0.61 | -0.39 | -0.25 | -0.23 | -0.12 | 0.3 | 0.37 | 0.27 | -0.27 | -0.25 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4455 | -0.5605 | -0.9201 | -1.0467 | -1.0125 | -1.0284 | 0.1909 | 0.1872 | 0.573 | -0.0931 | 0.9938 | 0.1405 | 0.0216 | -0.3447 | -0.2714 | 0.1518 | 0.1481 | -0.3844 |
Actin | 0.0479 | 0.0088 | 0.0162 | 0.0217 | 0.0037 | 0.0073 | 0.016 | 0.0007 | 0.0233 | 0.0107 | 0.0166 | 0.0077 | 0.0432 | 0.003 | 0.0092 | 0.0142 | 0.0057 | 0.0028 |
Bud | 0.0017 | 0.002 | 0.0003 | 0.0002 | 0.0021 | 0.0002 | 0.0025 | 0.0033 | 0.0049 | 0.0086 | 0.001 | 0.0022 | 0.0018 | 0.0038 | 0.0116 | 0.0006 | 0.0323 | 0.002 |
Bud Neck | 0.0289 | 0.0034 | 0.0125 | 0.0125 | 0.0091 | 0.0006 | 0.0008 | 0.0007 | 0.0038 | 0.0004 | 0.0127 | 0.002 | 0.0037 | 0.0002 | 0.0041 | 0.0237 | 0.0007 | 0.0017 |
Bud Periphery | 0.0032 | 0.0032 | 0.0006 | 0.0003 | 0.0053 | 0.0004 | 0.0032 | 0.0032 | 0.0055 | 0.0114 | 0.0011 | 0.0041 | 0.0075 | 0.0034 | 0.0069 | 0.0005 | 0.0064 | 0.0009 |
Bud Site | 0.0294 | 0.0039 | 0.0013 | 0.0029 | 0.0165 | 0.0004 | 0.0076 | 0.0216 | 0.0142 | 0.0026 | 0.0289 | 0.0018 | 0.0057 | 0.0109 | 0.0078 | 0.009 | 0.0055 | 0.0068 |
Cell Periphery | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.001 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Cytoplasm | 0.0391 | 0.0464 | 0.0425 | 0.0686 | 0.0499 | 0.0726 | 0.0862 | 0.1385 | 0.122 | 0.1199 | 0.1222 | 0.1564 | 0.0591 | 0.1334 | 0.1012 | 0.1289 | 0.1586 | 0.2049 |
Cytoplasmic Foci | 0.0307 | 0.0155 | 0.0043 | 0.0028 | 0.0214 | 0.0162 | 0.0205 | 0.0075 | 0.0075 | 0.0283 | 0.0235 | 0.0127 | 0.0199 | 0.0077 | 0.0055 | 0.0297 | 0.0171 | 0.0096 |
Eisosomes | 0.0009 | 0.0007 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0063 | 0.0011 | 0.0009 | 0.0009 | 0.002 | 0.0007 | 0.0044 | 0.0013 | 0.0017 | 0.002 | 0.0043 | 0.0011 | 0.0075 | 0.0021 | 0.0019 | 0.0021 | 0.0009 | 0.003 |
Endosome | 0.0224 | 0.0016 | 0.002 | 0.0016 | 0.0316 | 0.0031 | 0.0088 | 0.0022 | 0.0115 | 0.0141 | 0.0266 | 0.0024 | 0.0155 | 0.0028 | 0.0065 | 0.0191 | 0.0061 | 0.0062 |
Golgi | 0.0057 | 0.0006 | 0.0004 | 0.0015 | 0.0037 | 0.0018 | 0.0028 | 0.0002 | 0.0038 | 0.0132 | 0.0103 | 0.0016 | 0.008 | 0.0005 | 0.0008 | 0.01 | 0.0131 | 0.0036 |
Lipid Particles | 0.0045 | 0.0012 | 0.0007 | 0.0008 | 0.0032 | 0.003 | 0.0071 | 0.0004 | 0.0054 | 0.0099 | 0.0418 | 0.0027 | 0.0135 | 0.0007 | 0.0007 | 0.0139 | 0.0028 | 0.0009 |
Mitochondria | 0.005 | 0.0018 | 0.0006 | 0.0005 | 0.0022 | 0.0007 | 0.0033 | 0.0006 | 0.0072 | 0.0198 | 0.0249 | 0.0023 | 0.0067 | 0.0005 | 0.001 | 0.0025 | 0.0147 | 0.0016 |
None | 0.7273 | 0.9038 | 0.912 | 0.8811 | 0.8186 | 0.8858 | 0.7773 | 0.8098 | 0.7735 | 0.7029 | 0.5914 | 0.7884 | 0.7706 | 0.8225 | 0.8281 | 0.7212 | 0.7261 | 0.7454 |
Nuclear Periphery | 0.0221 | 0.0006 | 0.0004 | 0.0006 | 0.0028 | 0.0008 | 0.0165 | 0.0007 | 0.0026 | 0.0026 | 0.0346 | 0.0018 | 0.0108 | 0.0008 | 0.0036 | 0.0012 | 0.0004 | 0.001 |
Nucleolus | 0.001 | 0.0006 | 0.0005 | 0.0003 | 0.0029 | 0.0003 | 0.0018 | 0.0008 | 0.0014 | 0.0008 | 0.0039 | 0.0009 | 0.0005 | 0.0005 | 0.0007 | 0.0005 | 0.0002 | 0.0003 |
Nucleus | 0.007 | 0.001 | 0.0012 | 0.0006 | 0.0056 | 0.0005 | 0.0164 | 0.0033 | 0.0023 | 0.0036 | 0.0097 | 0.0035 | 0.004 | 0.0021 | 0.0019 | 0.0023 | 0.0024 | 0.0023 |
Peroxisomes | 0.0067 | 0.0007 | 0.0005 | 0.0005 | 0.0013 | 0.0015 | 0.0071 | 0.0004 | 0.002 | 0.0398 | 0.0227 | 0.0012 | 0.011 | 0.0007 | 0.0006 | 0.0137 | 0.0034 | 0.002 |
Punctate Nuclear | 0.0044 | 0.0017 | 0.0009 | 0.0013 | 0.0058 | 0.0032 | 0.0122 | 0.0022 | 0.0029 | 0.0025 | 0.0174 | 0.0055 | 0.0059 | 0.0028 | 0.0059 | 0.0047 | 0.0019 | 0.0039 |
Vacuole | 0.003 | 0.0009 | 0.0011 | 0.0005 | 0.0103 | 0.0003 | 0.0029 | 0.0014 | 0.0026 | 0.0053 | 0.0026 | 0.001 | 0.0023 | 0.0009 | 0.0011 | 0.0014 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0012 | 0.0002 | 0.0012 | 0.0011 | 0.0033 | 0.0004 | 0.0012 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.5872 | 6.6226 | 8.6722 | 7.4975 | 6.7802 | 7.4042 | 9.8472 | 7.8425 | 7.4242 | 10.0088 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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