Standard name
Human Ortholog
Description Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.9 0.85 0.86 0.8 0.7 0.69 0.58 0.6 0.51 0.51 0.88 0.87 0.9 0.73 0.74 0.87 0.94 0.94 0.82 0.87 0.82 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.29 0.2 0.47 0.37 0.57 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.18 0.18 0.15 0.15 0.14 0.1 0.12 0.07 0.15 0.12 0.19 0.09 0.33 0.2 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2
Bud 0 0 0 0 0 3 0 2 10 3 6 7 1 0 0 0 0 0 2 0 1 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2 0 4 1 0 1 2 1 0 2 4 3 5 2 0 0 0 0 0 0
Cytoplasm 303 38 114 188 250 209 285 231 164 153 136 109 189 296 370 119 75 75 296 57 125 163 280 219
Endoplasmic Reticulum 1 0 2 3 0 2 1 2 3 3 1 0 2 1 1 4 2 1 0 0 0 2 2 5
Endosome 0 0 6 6 8 2 4 6 0 3 0 2 2 9 17 10 4 3 0 0 9 1 16 9
Golgi 0 0 0 0 0 1 0 1 0 0 0 0 0 3 2 6 5 1 0 0 1 2 3 1
Mitochondria 1 0 1 0 1 35 119 67 133 95 150 86 0 0 5 0 3 2 0 0 3 1 3 2
Nucleus 1 0 4 5 2 6 14 11 12 6 11 11 2 3 1 4 0 1 8 1 2 7 10 9
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 1 0 0 2 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 2 0 0 5 0 0 1 1 1 0 2 5 4 2 2 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 2 0 0 3 1 0 0 1 2 0 5 1 4 0 0 0 1 0 6 5
Vac/Vac Membrane 1 0 6 39 53 39 60 48 28 31 18 31 25 64 36 54 20 9 0 1 3 2 6 8
Unique Cell Count 306 38 127 220 292 262 409 335 284 256 265 213 214 341 413 164 101 86 316 61 152 188 343 278
Labelled Cell Count 307 38 133 245 319 297 492 375 353 296 325 251 223 380 450 206 120 96 316 61 152 188 343 278


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 10.7 8.2 8.7 8.0 6.0 5.5 5.7 4.9 5.1 4.7 5.0 9.8 9.4 8.5 14.9 16.1 15.4 9.6 9.9 9.8
Std Deviation (1e-4) 1.3 2.0 1.5 1.4 1.5 1.5 1.3 1.3 1.1 1.2 1.4 1.3 1.6 1.6 1.3 3.7 3.1 3.8 1.7 1.9 1.7
Intensity Change (Log2) 0.09 -0.02 -0.45 -0.56 -0.51 -0.75 -0.67 -0.81 -0.72 0.26 0.21 0.06 0.87 0.98 0.92 0.24 0.28 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -1.2 -2.5 -4.5 -4.6 -6.4 -6.0 -7.4 -7.2 -0.4 -0.9 -0.1 -3.6 -3.1 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 -0.3 0.5 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 6.7 5.2 0 7.7 0 8.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.5 3.6 2.9 3.0 2.9 1.7 2.3 0.8 2.8 2.2 3.8 1.5 5.9 3.6 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3174 5.5881 5.004 4.591 4.6581 4.9598 4.2369 5.4271 4.9186 3.5825 4.8257 4.9981 5.4415 6.215 6.0536 5.8532 4.1121 6.062
Actin 0.0127 0.0003 0.0036 0.0181 0.0021 0.0002 0.022 0.0008 0.0013 0.0024 0.0002 0.0124 0.0126 0.001 0.0052 0.0002 0.0318 0.0014
Bud 0.0008 0.0005 0.0011 0.0019 0.0002 0.0001 0.0045 0.0078 0.0069 0.0004 0.0001 0.0004 0.0007 0.0004 0.0003 0.0001 0.0017 0.0002
Bud Neck 0.002 0.0006 0.001 0.0008 0.0014 0.0017 0.0016 0.0006 0.0011 0.0015 0.0012 0.0035 0.0012 0.0005 0.0005 0.0006 0.0068 0.002
Bud Periphery 0.0008 0.0001 0.0004 0.0029 0.0002 0 0.0015 0.0033 0.0014 0.0002 0 0.0004 0.0007 0.0001 0.0003 0 0.002 0.0001
Bud Site 0.0011 0.0012 0.0017 0.0016 0.0004 0.0001 0.0064 0.0057 0.001 0.0005 0.0001 0.0007 0.002 0.0021 0.0007 0.0001 0.0023 0.0002
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0.0003 0
Cytoplasm 0.3514 0.4841 0.3845 0.219 0.3481 0.3396 0.4498 0.6184 0.4273 0.3818 0.5588 0.5504 0.4527 0.6525 0.4014 0.5197 0.2987 0.4289
Cytoplasmic Foci 0.0183 0.0018 0.0078 0.0141 0.0077 0.0018 0.0364 0.005 0.0123 0.0246 0.0075 0.0069 0.0136 0.0043 0.0059 0.0011 0.046 0.0047
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0009 0
Endoplasmic Reticulum 0.0087 0.0028 0.0046 0.0021 0.0044 0.002 0.0051 0.0028 0.0042 0.0026 0.0022 0.0046 0.0053 0.0026 0.0021 0.0011 0.0203 0.0028
Endosome 0.0436 0.0031 0.0113 0.013 0.0221 0.0037 0.0357 0.0038 0.01 0.0206 0.0041 0.0094 0.0188 0.0034 0.0039 0.0008 0.078 0.0069
Golgi 0.0052 0.0001 0.0026 0.003 0.0033 0.0001 0.0072 0.0003 0.0007 0.0069 0.0002 0.0019 0.0038 0.0005 0.0014 0 0.0204 0.0011
Lipid Particles 0.0124 0 0.0027 0.0019 0.0145 0 0.0163 0.0001 0.0005 0.0737 0 0.001 0.0065 0.0002 0.0039 0 0.0633 0.0024
Mitochondria 0.0055 0.0005 0.0037 0.0038 0.0076 0.0004 0.0066 0.0074 0.0012 0.0021 0.0003 0.0133 0.0085 0.0093 0.012 0.0003 0.0339 0.0016
None 0.0137 0.0037 0.0077 0.0022 0.0103 0.0044 0.0341 0.0148 0.0143 0.0063 0.0092 0.0035 0.0141 0.0049 0.0091 0.0009 0.0133 0.0011
Nuclear Periphery 0.031 0.0197 0.0195 0.0138 0.0487 0.0159 0.032 0.0145 0.0272 0.015 0.0109 0.0204 0.0253 0.0137 0.0155 0.0114 0.0706 0.0187
Nucleolus 0.0012 0.0005 0.0011 0.0008 0.0016 0.0005 0.0013 0.0005 0.0005 0.0008 0.0003 0.0005 0.0015 0.0003 0.0003 0.0002 0.0023 0.0004
Nucleus 0.4558 0.4705 0.5268 0.6668 0.4673 0.6186 0.3107 0.2987 0.4777 0.4179 0.3973 0.3601 0.4094 0.296 0.5268 0.4606 0.2535 0.5149
Peroxisomes 0.0087 0.0001 0.0015 0.0178 0.0065 0.0001 0.0141 0.0002 0.001 0.0293 0.0001 0.0014 0.0087 0.0007 0.0054 0 0.0241 0.0017
Punctate Nuclear 0.0201 0.0073 0.0146 0.0041 0.045 0.0083 0.0095 0.0128 0.0079 0.0115 0.0067 0.0055 0.0069 0.0056 0.0035 0.0019 0.0148 0.0088
Vacuole 0.0049 0.0027 0.0029 0.0107 0.0043 0.002 0.0031 0.0019 0.0021 0.0015 0.0006 0.0026 0.0058 0.0015 0.0012 0.0008 0.0095 0.0016
Vacuole Periphery 0.0019 0.0005 0.0008 0.0016 0.0041 0.0004 0.0016 0.0004 0.0013 0.0006 0.0002 0.0008 0.0015 0.0004 0.0004 0.0002 0.0056 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 94.1267 60.311 56.3026 63.6434 65.605 47.853 53.1119 49.7875 45.5668 72.5471
Translational Efficiency 0.9182 1.3455 1.2034 0.9147 1.0918 1.4382 1.4176 1.0662 1.2503 1.1427

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1938 368 2671 1492 1877 1678 145 1352 3815 2046 2816 2844

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 957.56 996.44 1294.52 1413.20 965.87 1079.01 1456.30 1447.23 961.65 1064.16 1302.85 1429.38
Standard Deviation 143.62 198.53 193.49 232.85 147.60 176.64 196.56 243.17 145.65 183.53 196.92 238.42
Intensity Change Log 2 0.057420 0.434982 0.561531 0.159807 0.592407 0.583393 0.109743 0.516180 0.572551

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000204 0.001082 0.000224 0.001040 0.000245 0.000467 0.001130 0.009038 0.000224 0.000577 0.000270 0.004842
Bud Neck 0.003626 0.004767 0.002591 0.004490 0.002592 0.003981 0.004605 0.007673 0.003117 0.004122 0.002694 0.006003
Bud Site 0.004661 0.017547 0.002052 0.012587 0.003408 0.012561 0.008333 0.040394 0.004044 0.013457 0.002376 0.025806
Cell Periphery 0.000076 0.000140 0.000058 0.000176 0.000082 0.000092 0.000262 0.000128 0.000079 0.000101 0.000068 0.000153
Cytoplasm 0.672374* 0.673369* 0.487510* 0.513769* 0.680494* 0.702634* 0.403649* 0.384217* 0.676369* 0.697370* 0.483192* 0.452182*
Cytoplasmic Foci 0.108047 0.066585 0.001406 0.014197 0.051280 0.058524 0.012081 0.048007 0.080117 0.059974 0.001955 0.030270
Eisosomes 0.000026 0.000029 0.000004 0.000012 0.000017 0.000018 0.000033 0.000015 0.000022 0.000020 0.000006 0.000013
Endoplasmic Reticulum 0.002641 0.002539 0.000659 0.000847 0.002975 0.001620 0.003986 0.000446 0.002805 0.001785 0.000830 0.000656
Endosome 0.004642 0.009841 0.000193 0.007457 0.002401 0.005470 0.003462 0.014287 0.003540 0.006256 0.000362 0.010704
Golgi 0.002103 0.001772 0.000047 0.007226 0.000655 0.001827 0.000365 0.029410 0.001390 0.001817 0.000063 0.017772
Lipid Particles 0.002060 0.001836 0.000231 0.001379 0.001051 0.001014 0.002699 0.002315 0.001563 0.001162 0.000358 0.001824
Mitochondria 0.002156 0.000501 0.000753 0.004363 0.002268 0.001518 0.000513 0.004836 0.002211 0.001335 0.000741 0.004588
Mitotic Spindle 0.002060 0.006862 0.007599 0.147076* 0.004230 0.011009 0.032134 0.301437* 0.003127 0.010263 0.008862 0.220457*
None 0.003294 0.008813 0.009072 0.006995 0.003629 0.004093 0.005150 0.005800 0.003459 0.004942 0.008870 0.006427
Nuclear Periphery 0.000353 0.000306 0.000915 0.000839 0.000462 0.000430 0.002094 0.000614 0.000406 0.000408 0.000975 0.000732
Nuclear Periphery Foci 0.002012 0.004980 0.000370 0.005107 0.002784 0.002778 0.006931 0.003573 0.002392 0.003174 0.000708 0.004378
Nucleolus 0.000493 0.001823 0.000120 0.000556 0.000401 0.000431 0.000264 0.000632 0.000448 0.000682 0.000128 0.000592
Nucleus 0.140238* 0.126859 0.472008* 0.221696* 0.209543* 0.130606* 0.341236* 0.101888 0.174336* 0.129932 0.465274* 0.164741*
Peroxisomes 0.001245 0.003120 0.000020 0.000553 0.000634 0.001593 0.000293 0.004434 0.000944 0.001868 0.000034 0.002398
Vacuole 0.046685 0.065179 0.014093 0.045147 0.030397 0.058663 0.170522 0.035489 0.038671 0.059835 0.022147 0.040555
Vacuole Periphery 0.001004 0.002049 0.000075 0.004488 0.000454 0.000672 0.000258 0.005369 0.000734 0.000919 0.000085 0.004907

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.17 -0.90 -5.88 1.27 -4.63 -3.38 -8.22 -2.25 -1.94 -0.50 -5.11 -1.70 -3.00 -1.95 -2.71
Bud Neck -1.41 0.62 -7.23 -3.09 -7.86 -2.62 -5.81 -9.46 -7.81 -1.03 -2.55 -1.08 -11.81 -8.65 -10.26
Bud Site -5.42 4.59 -8.88 1.05 -10.74 -6.60 -6.73 -12.98 -7.48 -8.81 -7.99 2.75 -15.63 -5.59 -16.50
Cell Periphery -4.29 1.54 -7.80 -1.95 -8.19 -1.34 -9.40 -7.46 -5.00 7.11 -3.39 -1.07 -10.35 -6.12 -9.27
Cytoplasm 1.55 34.40 28.89 14.71 1.47 -0.16 25.00 31.50 29.92 -7.22 0.14 43.88 42.67 35.70 3.24
Cytoplasmic Foci 5.77 31.07 19.67 5.88 -11.62 -2.07 18.61 0.96 2.65 -12.80 6.49 37.59 15.43 7.53 -19.12
Eisosomes -1.41 15.16 9.03 4.57 -7.51 -1.73 -9.40 2.85 5.30 10.69 -0.35 12.58 8.39 7.22 -5.30
Endoplasmic Reticulum -0.14 6.77 7.63 4.48 2.92 3.53 -4.15 8.09 9.42 9.01 3.61 7.62 11.03 9.37 9.40
Endosome -2.41 11.02 3.18 3.45 -6.47 -5.30 -3.44 -3.75 0.25 -0.91 -4.42 12.17 -0.87 3.28 -9.10
Golgi 0.94 5.22 0.65 -0.19 -3.11 -2.42 2.33 -3.13 -2.43 -3.32 -0.57 6.35 -2.59 -2.21 -4.37
Lipid Particles 0.21 6.99 -6.34 -5.20 -14.43 -0.24 -8.04 -10.32 -10.22 0.21 2.06 7.04 -11.64 -13.77 -17.81
Mitochondria 3.03 2.23 1.15 -2.39 -1.23 1.00 1.94 1.12 0.21 -0.86 1.68 2.76 1.58 -0.21 -1.26
Mitotic Spindle -1.85 -5.58 -20.41 -19.05 -19.31 -3.03 -2.14 -26.06* -25.19* -20.85* -4.08 -5.12 -32.69 -30.92* -31.23*
None -1.84 -17.21 -9.19 0.02 2.76 -0.38 -0.44 -6.65 -4.60 -3.59 -1.68 -18.68 -11.17 -4.14 3.34
Nuclear Periphery -0.36 -22.73 -18.41 -17.93 -4.28 -0.58 -10.40 -13.30 -14.15 7.39 -1.92 -24.50 -22.55 -21.74 -0.25
Nuclear Periphery Foci -3.86 9.43 -8.30 -2.63 -10.23 -0.75 -4.45 -5.19 -4.77 1.56 -3.51 7.64 -9.67 -6.83 -12.84
Nucleolus -2.39 4.15 -4.57 0.89 -7.88 -0.29 0.24 -3.92 -5.57 -6.30 -1.72 3.47 -5.96 -3.88 -11.35
Nucleus 0.51 -62.64 -11.54 -7.65 40.80 12.23 -6.44 13.21 2.01 10.19 7.94 -59.64 0.45 -6.62 54.86
Peroxisomes -2.41 7.42 4.26 3.38 -7.96 -3.43 2.68 -4.54 -1.83 -6.17 -3.49 8.50 -1.90 1.91 -8.38
Vacuole -5.11 4.88 -15.73 -5.51 -18.58 -11.98 -12.81 -16.80 -7.75 8.82 -11.55 -3.91 -23.03 -10.86 -17.90
Vacuole Periphery -1.26 4.70 0.01 1.17 -2.86 -1.38 1.55 -1.68 -1.23 -2.22 -0.62 5.58 -1.17 -0.78 -3.92
Standard name
Human Ortholog
Description Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Meu1

Meu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Meu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available