Standard name
Human Ortholog
Description Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – – – – – – –
Cell Periphery – 0.18 – 0.65 0.6 0.49 0.53 – 0.69 0.41 0.52 0.65 – 0.5 – – – – 0.3 0.4 0.29 0.25 0.35 0.25
Cytoplasm – 0.94 – 0.74 0.55 0.64 0.6 – 0.43 0.68 0.48 0.5 – 0.88 – – – – 0.67 0.52 0.59 0.63 0.5 0.58
Endoplasmic Reticulum – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0.07 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Golgi – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Mitochondria – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0.08
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0 0.12 0 0 – 0 0.05 0 0 – 0 – – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – – – – – – –
Cell Periphery – 3 – 50 36 34 30 – 37 15 12 13 – 8 – – – – 19 19 19 5 17 11
Cytoplasm – 16 – 57 33 45 34 – 23 25 11 10 – 14 – – – – 42 26 39 14 24 27
Endoplasmic Reticulum – 0 – 0 0 0 0 – 1 0 0 0 – 0 – – – – 0 0 0 1 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Golgi – 0 – 0 2 1 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Mitochondria – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 1 2 0 2 3
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 1 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 1 0 – 0 – – – – 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0 7 0 0 – 1 2 0 0 – 0 – – – – 0 0 0 0 0 0
Unique Cell Count 17 77 60 70 57 54 37 23 20 16 64 50 67 23 50 47
Labelled Cell Count 19 107 78 80 64 62 42 24 23 22 64 50 67 23 50 47


Ambiguous, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 829.2 – 765.0 632.9 818.7 762.5 – 771.5 757.5 771.6 762.6 – 721.2 – – – – – – –
Std Deviation (1e-4) – 140.3 – 153.4 251.7 206.5 114.3 – 142.1 204.6 127.4 123.4 – 106.5 – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0001 0.0002 0.0003 0.0007 0.0002 0.0005 0.0014 0.0014 0.0015 0.0015 0.0029 0.0004 0.0046 0.0007 0.0147 0.0003 0.0071 0.0003
Bud 0 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0011 0.0005 0.0005 0.0003 0.0002 0.003 0.0068 0.0011 0.0006 0.0012 0.0004
Bud Neck 0 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0008 0.0002
Bud Periphery 0 0 0 0 0.0001 0 0.0003 0.0006 0.0003 0.0002 0.0003 0.0001 0.0024 0.0014 0.0008 0.0009 0.002 0.0002
Bud Site 0 0 0.0001 0 0 0 0.0012 0.0003 0.0002 0.0002 0.0003 0.0001 0.003 0.0057 0.0018 0.0002 0.0016 0.0001
Cell Periphery 0 0.0002 0.0003 0.0002 0.0007 0.0001 0.0039 0.0013 0.0034 0.0009 0.0063 0.0002 0.0033 0.0038 0.0016 0.0012 0.0064 0.0005
Cytoplasm 0.1183 0.4273 0.1995 0.0502 0.1099 0.0337 0.0667 0.2909 0.0519 0.0159 0.0105 0.0119 0.0404 0.3526 0.0039 0.0029 0.0031 0.0879
Cytoplasmic Foci 0 0 0 0 0.0001 0 0.0112 0.0001 0.0026 0 0.0025 0.0018 0.0115 0.0005 0.037 0.0669 0.0032 0.0006
Eisosomes 0 0 0.0001 0 0 0 0.0161 0.0002 0.0016 0.0003 0.0043 0.0001 0.0013 0.0003 0.0055 0.001 0.0052 0.0001
Endoplasmic Reticulum 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0003 0.0002 0.0006 0 0.0011 0.0003 0.0005 0.0001 0.0003 0
Endosome 0 0 0 0 0.0002 0 0.0002 0.0001 0.0004 0.0001 0.0034 0 0.0042 0 0.0057 0.0003 0.0019 0
Golgi 0 0 0 0 0 0 0.0003 0 0.0004 0 0.0026 0 0.0007 0 0.0055 0.0001 0.0026 0
Lipid Particles 0 0 0 0 0 0 0.0032 0 0.0032 0 0.0105 0.0001 0.0049 0.0002 0.0148 0.0035 0.0184 0.0001
Mitochondria 0.0031 0.0099 0.0227 0.011 0.0171 0.0056 0.0707 0.0289 0.0421 0.0444 0.0508 0.0107 0.0583 0.0168 0.1147 0.0995 0.0966 0.0203
None 0.0002 0.0005 0.0013 0.0008 0.0006 0.0002 0.0093 0.0013 0.0037 0.002 0.0076 0.0011 0.0397 0.0037 0.0121 0.0363 0.0095 0.001
Nuclear Periphery 0.0731 0.0559 0.1291 0.2819 0.181 0.109 0.0554 0.0883 0.1317 0.1354 0.2163 0.0615 0.0589 0.048 0.0318 0.0189 0.0315 0.0308
Nucleolus 0.0336 0.0213 0.0474 0.0334 0.0082 0.017 0.3664 0.0777 0.2114 0.0437 0.4705 0.1366 0.3474 0.0279 0.3589 0.4868 0.3571 0.1491
Nucleus 0.771 0.4757 0.5912 0.6176 0.6129 0.8286 0.3691 0.4854 0.5113 0.7412 0.1141 0.7628 0.3511 0.5258 0.3644 0.2497 0.3809 0.6976
Peroxisomes 0 0 0.0001 0 0.0001 0 0.0014 0.0002 0.0004 0.0001 0.0006 0.0001 0.0012 0.0001 0.0035 0.0026 0.0012 0.0002
Punctate Nuclear 0 0.0001 0.0003 0.0002 0.0001 0.0001 0.0037 0.0002 0.0019 0.0002 0.0069 0.0032 0.0286 0.0005 0.008 0.0032 0.0022 0.0002
Vacuole 0.0001 0.0009 0.0011 0.0003 0.0095 0.0003 0.0039 0.0049 0.0069 0.0015 0.0117 0.0008 0.0148 0.0021 0.0028 0.0045 0.0113 0.0015
Vacuole Periphery 0.0005 0.0076 0.0062 0.0034 0.0586 0.0047 0.0149 0.0167 0.0239 0.0116 0.077 0.0082 0.0192 0.0027 0.0106 0.0203 0.0558 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10610.4858 11961.2685 14296.0664 12291.003 12403.2885 11875.236 13502.6284 12682.7842 14456.8009 12040.0934
Translational Efficiency 1.2948 1.256 1.1625 1.1445 1.169 1.2333 1.3659 1.3519 1.2544 1.4974

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdc1

Pdc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available