Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.21 0.13 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.17 0.13 0.15 0 0 0 0 0 0
Cytoplasm 0.6 0.4 0.5 0.44 0.62 0.44 – 0.39 0.36 0.41 0.43 0.4 0.54 0.64 0.72 0.27 0.27 0.26 0.19 0.09 0.1 0.12 0.17 0.19
Endoplasmic Reticulum 0.21 0.56 0.26 0.34 0.19 0.25 – 0.21 0.22 0.17 0.17 0.14 0.45 0.37 0.24 0 0 0 0.61 0.7 0.65 0.6 0.55 0.51
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.19 0.13 0.17 0 0 0 0 0 0
Mitochondria 0.06 0 0.27 0.46 0.18 0.48 – 0.44 0.55 0.47 0.52 0.53 0 0 0 0.06 0.07 0.05 0.06 0.05 0.12 0.12 0.11 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.09 0.07 0 0.1 0.05 – 0.08 0.07 0.11 0.05 0.06 0 0 0 0.29 0.31 0.38 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 1 0 2 0 – 2 2 2 3 3 0 1 1 45 38 31 8 4 8 4 8 9
Bud 3 3 5 2 3 6 – 10 11 9 16 16 0 0 0 2 2 3 13 3 0 6 4 11
Bud Neck 0 0 1 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 8 2 1 1 1 3
Bud Site 1 0 2 0 0 0 – 2 0 1 0 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 4 1 5 6 8 – 5 6 9 6 18 7 6 17 45 23 36 4 0 2 1 2 1
Cytoplasm 263 87 96 54 121 117 – 144 125 163 144 178 96 98 164 69 48 62 110 23 22 24 49 53
Endoplasmic Reticulum 91 122 49 42 38 65 – 77 76 67 57 60 80 57 54 3 8 5 349 180 148 121 161 142
Endosome 9 3 1 0 8 0 – 4 3 7 2 5 1 0 1 10 1 3 6 5 1 1 3 7
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 48 23 40 5 2 0 2 2 4
Mitochondria 24 1 51 56 35 126 – 161 194 186 175 236 2 3 2 15 13 12 34 13 27 23 33 17
Nucleus 2 1 1 0 0 0 – 6 1 2 1 3 0 1 0 2 4 5 1 0 0 0 0 0
Nuclear Periphery 1 1 1 1 0 3 – 10 19 11 2 8 1 0 0 0 1 2 3 3 4 2 2 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 1 1 1 1 1 0 0 0 2 1 2 4 0 1 0 2 1
Vac/Vac Membrane 79 20 13 0 20 14 – 29 26 43 17 27 2 1 5 75 56 88 19 8 4 7 17 21
Unique Cell Count 435 216 192 123 196 265 369 350 398 337 444 177 154 227 258 179 234 576 256 229 202 295 281
Labelled Cell Count 479 248 222 160 234 339 452 464 501 425 557 190 167 245 318 219 291 576 256 229 202 295 281


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.2 4.5 4.9 5.8 4.7 – 5.0 4.6 4.9 4.8 4.8 6.4 6.8 6.4 11.6 11.1 11.0 6.5 6.6 7.0
Std Deviation (1e-4) 1.2 1.6 1.1 1.1 2.1 1.3 – 1.7 1.5 1.4 1.7 1.6 1.4 1.6 1.5 2.7 2.5 2.4 1.7 1.8 1.9
Intensity Change (Log2) – – – 0.11 0.37 0.07 – 0.15 0.02 0.12 0.11 0.08 0.51 0.61 0.52 1.37 1.3 1.3 0.54 0.55 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 5.9 6.5 5.0
Bud 0 0 0 – 0.1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 3.5 5.9 4.8 5.4
Cytoplasm -1.1 2.3 -1.2 – -2.5 -3.2 -2.1 -1.6 -2.3 0.8 2.5 4.7 -5.1 -4.6 -5.0
Endoplasmic Reticulum 0 -1.4 0 – -1.3 0 -2.5 0 0 4.0 2.3 -0.4 -8.0 -5.6 -7.2
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 6.3 5.1 6.0
Mitochondria 0 -2.1 0 – 4.0 0 4.7 0 0 -7.0 -6.3 -7.9 -6.2 -4.9 -6.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 1.2 -0.7 – 0.5 0.3 1.6 -0.8 -0.3 -2.7 -2.9 -2.3 5.9 6.1 7.4