Standard name
Human Ortholog
Description Subunit of the INO80 chromatin remodeling complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.06 0 0 0.06 0 0 0 0.08 0.16 0.08 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.12 0 0.28 0 0.15 0.08 0.08 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.94 0.92 0.92 0.9 0.87 0.87 0.74 0.81 0.7 0.73 0.69 0.71 0.94 0.86 0.75 0.87 0.85 0.78 0.87 0.86 0.85 0.81 0.71 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.14 0.18 0.19 0.26 0.24 0.21 0.31 0.41 0.43 0.6 0.39 0.17 0.12 0.12 0.12 0.13 0.15 0.09 0.05 0.09 0.12 0.19 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 0 2 1 0 0 2 0 0 0 1 0 0 0 2 2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 1 0 0 0 0 6 6 2 2 5 0 0 0 0 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 7 1 4 0 6 5 6 4 7 0 7 6 20 43 19 15 15 0 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 8 0 0 0 0 0 1
Endosome 0 1 1 0 0 0 0 0 0 0 0 0 0 2 1 1 0 2 2 3 0 2 2 8
Golgi 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 2 1
Mitochondria 3 5 0 0 14 4 25 6 26 9 5 11 2 0 1 10 12 9 0 0 0 1 1 4
Nucleus 148 195 146 124 98 135 67 170 124 83 45 113 151 207 201 208 186 180 142 191 146 137 169 166
Nuclear Periphery 0 0 0 0 1 1 0 0 1 2 0 0 0 1 1 2 3 3 0 0 0 0 0 2
Nucleolus 46 30 28 26 29 37 19 66 73 49 39 63 28 29 33 28 28 35 14 12 16 20 45 60
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 1 0 0 0 0 1 0 0 0 0 3 3 0 0 3 0 3 3 2 2 7
Vac/Vac Membrane 0 2 0 1 0 1 1 0 2 0 0 0 0 3 3 2 3 6 2 4 2 3 12 25
Unique Cell Count 158 212 158 138 113 156 90 211 176 114 65 160 161 241 267 238 220 231 164 222 173 171 240 280
Labelled Cell Count 198 241 180 157 142 185 117 257 240 153 92 202 187 265 288 273 252 264 164 222 173 171 240 280


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 8.5 10.0 9.6 6.2 8.0 4.6 7.2 6.2 6.7 6.0 7.4 9.3 9.9 9.4 7.3 9.1 8.8 10.9 11.1 11.0
Std Deviation (1e-4) 1.8 2.5 3.3 3.1 1.8 3.1 1.4 1.7 1.6 2.3 1.3 2.0 2.5 3.1 3.7 2.1 3.2 2.9 2.1 2.2 2.6
Intensity Change (Log2) – – – -0.07 -0.68 -0.33 -1.11 -0.47 -0.69 -0.58 -0.73 -0.45 -0.12 -0.02 -0.09 -0.45 -0.14 -0.19 0.12 0.15 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 3.4 5.1 3.3 2.9 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.5 0 0 0 5.0 0 0 3.4 0 0 0 0 3.0 0
Nucleus -0.8 -1.5 -1.7 -3.9 -3.2 -5.1 -4.4 -4.5 -5.0 0.5 -2.0 -4.4 -1.6 -2.3 -3.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 1.6 1.3 0 3.0 4.7 4.6 6.3 4.3 -0.1 -1.6 -1.5 -1.7 -1.3 -0.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7033 3.6454 3.6551 3.4528 2.8115 3.6969 9.0223 8.9778 8.5778 7.4922 7.2554 8.1074 6.0598 5.9621 5.3033 5.5227 5.0868 5.4872
Actin 0.0152 0 0 0 0 0 0.0389 0 0.0212 0 0.0113 0.0021 0.0011 0 0.0084 0.0006 0.006 0
Bud 0 0 0 0 0 0 0.0008 0 0.0001 0 0.0001 0 0.0006 0 0 0.0001 0.0004 0
Bud Neck 0.0002 0.0001 0 0 0.0003 0.0003 0.002 0 0.0001 0 0.0003 0.0003 0.0019 0 0.0001 0.0001 0.0007 0.0003
Bud Periphery 0.0001 0 0 0 0 0 0.001 0 0.0002 0 0.0003 0.0001 0.0012 0 0 0.0001 0.001 0.0001
Bud Site 0.0002 0 0 0 0 0 0.0017 0 0.0005 0 0.0003 0 0.0008 0 0.0004 0.0012 0.003 0.0001
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0003 0 0 0 0.0003 0
Cytoplasm 0.001 0 0 0 0 0 0.0104 0 0.0094 0 0.0029 0.0001 0.0018 0 0.0003 0.0026 0.0001 0.0001
Cytoplasmic Foci 0.0053 0 0 0 0 0 0.009 0 0.0058 0 0.0105 0.0008 0.0035 0 0.0128 0.0034 0.0064 0
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0002 0 0.0001 0 0 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0006 0 0 0 0 0 0.0038 0 0.0045 0 0.0012 0.0005 0.0013 0 0 0.0004 0.0001 0.0001
Endosome 0.0058 0 0 0 0 0 0.0123 0 0.0077 0 0.0105 0.0031 0.0045 0 0.0052 0.0076 0.0044 0.0001
Golgi 0.0016 0 0 0 0 0 0.0073 0 0.0064 0 0.0061 0.0012 0.0016 0 0.0027 0.0007 0.005 0
Lipid Particles 0.0045 0 0 0 0 0 0.038 0 0.0079 0 0.002 0.0025 0.0068 0 0.0024 0.0021 0.0152 0.0001
Mitochondria 0.0005 0 0 0.0001 0.0002 0 0.0035 0.0001 0.0055 0 0.0503 0.0013 0.0022 0 0.0013 0.0006 0.0462 0.0002
None 0.0048 0 0 0 0 0 0.0242 0 0.0028 0 0.0005 0.0002 0.0007 0 0.0001 0.0027 0.0002 0.0001
Nuclear Periphery 0.0034 0.0002 0.0002 0.0002 0.0006 0 0.0128 0.0001 0.0012 0 0.0019 0.0052 0.0094 0.0003 0.0001 0.0039 0.0014 0.0005
Nucleolus 0.0216 0.0276 0.0199 0.0059 0.2128 0.1028 0.0371 0.0269 0.0149 0.0135 0.1296 0.0634 0.0279 0.0174 0.0101 0.0108 0.082 0.058
Nucleus 0.9251 0.9718 0.9796 0.9937 0.7822 0.8966 0.7543 0.9726 0.9068 0.9864 0.7626 0.9101 0.9185 0.9821 0.9504 0.9448 0.8037 0.9393
Peroxisomes 0.0068 0 0 0 0 0 0.0082 0 0.0035 0 0.0037 0.008 0.0031 0 0.005 0.0007 0.0165 0
Punctate Nuclear 0.0025 0.0002 0.0002 0 0.0037 0.0001 0.0311 0.0003 0.0008 0 0.0054 0.0006 0.0084 0.0001 0.0004 0.0165 0.0011 0.0006
Vacuole 0.0003 0.0001 0 0 0 0 0.002 0 0.0003 0 0.0002 0.0001 0.0027 0 0.0001 0.0007 0.0028 0.0001
Vacuole Periphery 0.0003 0 0 0 0 0 0.0012 0 0.0003 0 0.0002 0.0002 0.0015 0 0 0.0003 0.0032 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.8415 26.1986 20.4125 31.069 31.3481 27.8261 29.4694 25.9239 20.0648 32.4085
Translational Efficiency 1.2055 1.3188 1.4341 0.7567 1.1293 1.3978 0.9592 1.0424 1.2572 1.0606

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
593 979 2620 2285 1827 2334 156 2016 2420 3313 2776 4301

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 820.12 957.32 1179.66 1177.98 944.31 1137.01 1249.44 1298.08 913.88 1083.91 1183.58 1234.27
Standard Deviation 168.94 218.36 197.29 196.01 156.26 183.87 197.29 239.05 168.17 211.26 197.94 225.37
Intensity Change Log 2 — 0.223166 0.524464 0.522408 — 0.267912 0.403949 0.459047 — 0.247287 0.461220 0.488844

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000544 0.000707 0.000427 0.000783 0.000360 0.000410 0.001151 0.000502 0.000405 0.000498 0.000468 0.000651
Bud Neck 0.003424 0.006608 0.013407 0.024782 0.005042 0.007663 0.009062 0.013355 0.004645 0.007352 0.013163 0.019426
Bud Site 0.002157 0.001370 0.000569 0.002825 0.000428 0.001038 0.001032 0.000983 0.000851 0.001136 0.000595 0.001961
Cell Periphery 0.000196 0.000169 0.000024 0.000161 0.000043 0.000107 0.000034 0.000052 0.000080 0.000125 0.000024 0.000110
Cytoplasm 0.001041 0.002712 0.000143 0.002088 0.000212 0.000616 0.000143 0.000809 0.000415 0.001235 0.000143 0.001488
Cytoplasmic Foci 0.000817 0.000352 0.000008 0.000868 0.000027 0.000151 0.000040 0.000151 0.000220 0.000210 0.000009 0.000532
Eisosomes 0.000058 0.000083 0.000068 0.000046 0.000075 0.000103 0.000098 0.000063 0.000071 0.000097 0.000070 0.000054
Endoplasmic Reticulum 0.002842 0.002211 0.000797 0.001873 0.001632 0.001962 0.001879 0.000859 0.001928 0.002036 0.000858 0.001398
Endosome 0.000157 0.000202 0.000022 0.001278 0.000045 0.000352 0.000043 0.000156 0.000072 0.000308 0.000023 0.000752
Golgi 0.000110 0.000424 0.000037 0.001055 0.000044 0.000121 0.000100 0.000433 0.000061 0.000211 0.000040 0.000764
Lipid Particles 0.000096 0.000284 0.000005 0.000139 0.000009 0.000447 0.000037 0.000008 0.000031 0.000399 0.000007 0.000077
Mitochondria 0.000931 0.001402 0.000852 0.002112 0.000436 0.000692 0.001291 0.001254 0.000557 0.000902 0.000877 0.001710
Mitotic Spindle 0.001416 0.000271 0.000415 0.007519 0.000057 0.001524 0.000178 0.001037 0.000390 0.001154 0.000401 0.004481
None 0.017794 0.005892 0.000447 0.003488 0.001131 0.001525 0.001692 0.000815 0.005214 0.002816 0.000517 0.002235
Nuclear Periphery 0.000054 0.000043 0.000062 0.000953 0.000033 0.000126 0.000064 0.000237 0.000038 0.000102 0.000062 0.000617
Nuclear Periphery Foci 0.000743 0.000748 0.000022 0.000590 0.000060 0.000133 0.000040 0.000073 0.000227 0.000315 0.000023 0.000348
Nucleolus 0.090197 0.077263 0.036015 0.021330 0.075301 0.063243 0.057912 0.025182 0.078951 0.067386 0.037246 0.023136
Nucleus 0.875467* 0.896525* 0.945967* 0.924819* 0.914094* 0.918277* 0.920794* 0.952744* 0.904629* 0.911849* 0.944552* 0.937908*
Peroxisomes 0.001350 0.001546 0.000455 0.000470 0.000753 0.000656 0.003832 0.000417 0.000899 0.000919 0.000645 0.000445
Vacuole 0.000488 0.000837 0.000121 0.001596 0.000096 0.000489 0.000450 0.000444 0.000192 0.000591 0.000139 0.001056
Vacuole Periphery 0.000120 0.000348 0.000138 0.001226 0.000122 0.000365 0.000128 0.000427 0.000122 0.000360 0.000137 0.000852

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.15 1.78 -3.26 -2.92 -12.65 -2.53 -10.57 -9.19 -4.96 8.56 -2.69 -2.52 -10.02 -7.84 -10.51
Bud Neck -3.90 -14.36 -16.98 -14.93 -9.81 -5.68 -4.41 -10.79 -7.03 -3.56 -6.80 -18.78 -20.19 -16.09 -8.27
Bud Site 0.66 1.28 -0.36 -2.84 -5.78 -3.02 -5.67 -2.29 0.25 0.44 -0.76 0.80 -2.65 -2.70 -5.67
Cell Periphery 0.47 3.57 1.02 0.73 -3.69 -2.54 0.71 -1.14 2.13 -1.85 -1.92 4.38 -0.84 1.06 -4.38
Cytoplasm -1.71 2.77 -2.26 0.49 -4.49 -1.38 0.61 -1.98 -0.56 -2.18 -2.30 1.84 -3.90 -0.68 -4.73
Cytoplasmic Foci 0.90 1.75 0.42 -0.79 -2.51 -1.55 -1.05 -1.36 0.02 -1.24 0.05 1.86 -0.95 -1.16 -2.86
Eisosomes -3.65 -2.44 3.40 6.23 8.56 -4.69 -3.79 2.96 7.08 5.91 -5.56 0.27 5.72 10.35 6.56
Endoplasmic Reticulum 1.86 6.82 2.73 0.95 -11.91 -2.82 -1.75 12.36 10.40 6.68 -0.76 12.06 4.56 5.27 -10.26
Endosome -0.85 2.59 -2.67 -2.45 -3.17 -2.52 -0.25 -1.23 1.34 -1.21 -2.73 3.49 -3.12 -1.46 -3.43
Golgi -1.27 2.55 -1.98 -0.90 -2.27 -1.75 -5.15 -1.34 -1.05 -1.06 -1.93 2.41 -2.51 -1.74 -2.62
Lipid Particles -0.98 2.04 -0.16 0.94 -2.86 -1.37 -5.38 0.08 1.37 5.21 -1.59 2.15 -1.37 1.44 -2.85
Mitochondria -0.96 0.36 -4.16 -2.10 -10.41 -3.60 -8.86 -10.43 -5.86 0.60 -2.44 -4.22 -11.99 -6.35 -10.44
Mitotic Spindle 0.96 0.72 -4.23 -6.17 -5.90 -2.28 -1.63 -2.69 -0.51 -1.60 -1.38 -0.40 -6.15 -5.02 -5.97
None 3.22 4.99 4.14 1.85 -7.79 -0.78 -1.16 1.15 1.39 1.76 2.40 5.35 3.39 1.15 -7.94
Nuclear Periphery 1.34 -0.53 -5.96 -6.07 -5.92 -1.71 -2.55 -2.42 -1.23 -1.80 -1.63 -3.00 -6.48 -5.36 -6.17
Nuclear Periphery Foci -0.04 1.67 0.35 0.41 -3.76 -1.25 0.89 -0.61 0.74 -1.33 -0.54 1.90 -0.91 -0.22 -4.02
Nucleolus 2.38 11.36 12.94 13.94 3.56 4.04 1.76 18.14 14.93 4.97 4.42 16.15 22.15 19.80 5.72
Nucleus -2.74 -10.22 -5.10 -3.08 8.32 -1.11 -0.10 -10.61 -9.11 -4.28 -2.16 -13.05 -7.77 -5.96 4.84
Peroxisomes -0.38 3.65 3.40 6.84 -0.78 1.82 -6.38 4.23 2.64 6.96 0.02 3.25 5.26 6.49 2.73
Vacuole -0.80 1.85 -2.65 -2.21 -4.32 -3.91 -7.25 -2.63 -0.36 -0.48 -3.36 1.20 -4.35 -2.64 -4.84
Vacuole Periphery -5.26 -0.51 -2.84 -2.08 -2.81 -3.66 -1.46 -1.93 -0.42 -1.84 -4.93 -1.78 -3.41 -2.01 -3.33
Standard name
Human Ortholog
Description Subunit of the INO80 chromatin remodeling complex
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ies3

Ies3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ies3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available