Standard name | |
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Human Ortholog | |
Description | Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 3 | – | 2 | 5 | 4 | 4 | – | – | – | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 7 | 2 | 1 | 13 | 11 | 6 | – | 7 | 2 | 6 | 1 | – | – | – | 27 | 13 | 24 | 1 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | – | 9 | 9 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 6 | 3 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 16 | 9 | 4 | 2 | 3 | 3 | 1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 22 | 1 | 0 | 0 | 1 | 11 | 6 | – | 25 | 27 | 52 | 26 | – | – | – | 7 | 14 | 17 | 2 | 1 | 0 | 0 | 0 | 0 |
Nucleus | 112 | 130 | 35 | 18 | 77 | 111 | 153 | – | 86 | 123 | 110 | 114 | – | – | – | 176 | 157 | 186 | 114 | 132 | 54 | 2 | 4 | 17 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 12 | 2 | 1 | 0 | 2 | 6 | 10 | – | 33 | 34 | 51 | 54 | – | – | – | 6 | 3 | 4 | 19 | 22 | 13 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 2 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 0 | – | – | – | 5 | 3 | 5 | 8 | 4 | 4 | 0 | 1 | 0 |
Vac/Vac Membrane | 6 | 15 | 5 | 0 | 1 | 3 | 1 | – | 0 | 0 | 0 | 2 | – | – | – | 19 | 15 | 24 | 11 | 9 | 14 | 0 | 0 | 4 |
Unique Cell Count | 142 | 155 | 44 | 19 | 93 | 127 | 169 | 120 | 157 | 152 | 147 | 236 | 204 | 245 | 165 | 183 | 97 | 9 | 13 | 31 | ||||
Labelled Cell Count | 167 | 160 | 47 | 19 | 96 | 143 | 181 | 156 | 193 | 223 | 202 | 267 | 225 | 278 | 165 | 183 | 97 | 9 | 13 | 31 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 7.0 | 6.4 | 5.9 | 7.9 | 6.5 | 6.6 | – | 5.5 | 5.7 | 5.5 | 5.6 | – | – | – | 7.7 | 7.8 | 7.5 | – | – | – |
Std Deviation (1e-4) | 1.2 | 1.0 | 1.1 | 0.8 | 2.2 | 1.7 | 1.4 | – | 1.6 | 1.2 | 1.5 | 1.4 | – | – | – | 1.8 | 1.3 | 1.6 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.1 | 0.32 | 0.02 | 0.04 | – | -0.21 | -0.15 | -0.2 | -0.2 | – | – | – | 0.28 | 0.28 | 0.23 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.3 | 3.0 | 4.5 | 3.0 | – | – | – | 0 | 0 | 0 |
Nucleus | 1.5 | 0.5 | 1.3 | 2.0 | – | -1.0 | -0.2 | -1.0 | -0.3 | – | – | – | -0.7 | -0.4 | -0.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 3.5 | 3.0 | 4.1 | 4.4 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.756 | 2.7007 | 2.4569 | 2.1489 | 1.804 | 2.3397 | 2.0943 | 2.049 | 2.0359 | 1.9603 | 1.9401 | 1.898 | 2.1192 | 2.8495 | 2.0057 | 2.3086 | 2.242 | 1.861 |
Actin | 0.0047 | 0.0007 | 0.0012 | 0 | 0.0019 | 0.002 | 0.0033 | 0.0001 | 0.0076 | 0.0057 | 0.0023 | 0.0023 | 0.0668 | 0.0125 | 0.0838 | 0 | 0.002 | 0.0007 |
Bud | 0.0008 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0021 | 0.0005 | 0.0001 | 0.0009 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0024 | 0.0002 | 0.0009 | 0.0001 | 0.0019 | 0.0014 | 0.0078 | 0.0001 | 0.0003 | 0.0016 | 0.0013 | 0.0012 | 0.0041 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0008 |
Bud Periphery | 0.0021 | 0.0002 | 0.0008 | 0 | 0.0012 | 0.0002 | 0.0006 | 0 | 0.0009 | 0.0035 | 0.002 | 0.0004 | 0.0011 | 0.001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0028 | 0.0003 | 0.0005 | 0 | 0.0005 | 0.0002 | 0.0055 | 0.0001 | 0.0025 | 0.0033 | 0.0007 | 0.0003 | 0.0128 | 0.0022 | 0.0017 | 0.0001 | 0.0007 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0118 | 0.0017 | 0.0046 | 0.0004 | 0.0059 | 0.0002 | 0.0084 | 0.0001 | 0.0008 | 0.0032 | 0.0026 | 0.0002 | 0.0369 | 0.0035 | 0.0027 | 0.0005 | 0.0022 | 0.0001 |
Cytoplasmic Foci | 0.0083 | 0.0004 | 0.0128 | 0.0072 | 0.0015 | 0.0022 | 0.0093 | 0.0008 | 0.0093 | 0.0103 | 0.0113 | 0.0021 | 0.0602 | 0.0003 | 0.0028 | 0 | 0.0339 | 0.0055 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0.0003 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0003 | 0.0022 | 0.0001 | 0.0021 | 0.0004 | 0.0016 | 0 | 0.0005 | 0.0024 | 0.002 | 0.0003 | 0.0078 | 0.0005 | 0.0113 | 0 | 0.0016 | 0.0001 |
Endosome | 0.0205 | 0.0009 | 0.0187 | 0.0308 | 0.0204 | 0.0087 | 0.0118 | 0.0018 | 0.0089 | 0.0526 | 0.0328 | 0.0015 | 0.053 | 0.0009 | 0.0182 | 0 | 0.0689 | 0.025 |
Golgi | 0.0056 | 0.0004 | 0.0047 | 0.0008 | 0.0015 | 0.0062 | 0.0045 | 0.0009 | 0.0022 | 0.0221 | 0.0099 | 0.0009 | 0.0353 | 0.0002 | 0.0093 | 0 | 0.0101 | 0.0192 |
Lipid Particles | 0.0175 | 0.0015 | 0.0044 | 0.0014 | 0.0009 | 0.0116 | 0.0227 | 0.0037 | 0.0047 | 0.0233 | 0.0136 | 0.0033 | 0.0225 | 0.0001 | 0.009 | 0 | 0.043 | 0.0079 |
Mitochondria | 0.023 | 0.0036 | 0.0183 | 0.0001 | 0.0383 | 0.0037 | 0.0018 | 0.0001 | 0.0026 | 0.0298 | 0.0085 | 0.0048 | 0.0113 | 0.0015 | 0.008 | 0.0003 | 0.0018 | 0.0048 |
None | 0.0021 | 0.0001 | 0.0011 | 0.0001 | 0.0012 | 0.0002 | 0.0105 | 0 | 0.0004 | 0.0006 | 0.0044 | 0.0003 | 0.0269 | 0.0001 | 0.0007 | 0.0002 | 0.0026 | 0 |
Nuclear Periphery | 0.0174 | 0.0056 | 0.0099 | 0.0034 | 0.0184 | 0.0039 | 0.0039 | 0.0005 | 0.0043 | 0.006 | 0.0218 | 0.0035 | 0.0176 | 0.0018 | 0.0194 | 0.0015 | 0.0065 | 0.0004 |
Nucleolus | 0.0338 | 0.0272 | 0.0153 | 0.0171 | 0.1248 | 0.0755 | 0.0218 | 0.0263 | 0.0143 | 0.039 | 0.1168 | 0.0596 | 0.0197 | 0.0329 | 0.0219 | 0.0399 | 0.0282 | 0.0482 |
Nucleus | 0.8194 | 0.9501 | 0.877 | 0.9308 | 0.7186 | 0.8646 | 0.8649 | 0.9643 | 0.9238 | 0.7633 | 0.7389 | 0.9059 | 0.5604 | 0.9389 | 0.8011 | 0.9557 | 0.7725 | 0.8833 |
Peroxisomes | 0.0049 | 0.0004 | 0.0027 | 0.0001 | 0.0003 | 0.0025 | 0.0088 | 0.0003 | 0.0095 | 0.008 | 0.0059 | 0.0071 | 0.0218 | 0.0001 | 0.0037 | 0 | 0.0068 | 0.0019 |
Punctate Nuclear | 0.0062 | 0.0054 | 0.0079 | 0.0065 | 0.0508 | 0.0144 | 0.006 | 0.0008 | 0.0031 | 0.0046 | 0.0157 | 0.0053 | 0.036 | 0.0004 | 0.0029 | 0.0012 | 0.0156 | 0.0006 |
Vacuole | 0.0063 | 0.0006 | 0.008 | 0.0009 | 0.0057 | 0.0009 | 0.0046 | 0.0001 | 0.0026 | 0.0142 | 0.0055 | 0.0005 | 0.0022 | 0.0016 | 0.0008 | 0.0001 | 0.0017 | 0.0005 |
Vacuole Periphery | 0.0034 | 0.0003 | 0.0082 | 0.0001 | 0.0036 | 0.0008 | 0.0012 | 0 | 0.001 | 0.0038 | 0.0031 | 0.0003 | 0.0015 | 0.0004 | 0.001 | 0 | 0.0012 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.4687 | 22.9009 | 27.5133 | 24.4677 | 20.957 | 23.1654 | 23.7064 | 30.326 | 35.5445 | 21.6296 |
Translational Efficiency | 1.3229 | 1.017 | 0.9076 | 1.2702 | 0.9608 | 0.7623 | 0.8445 | 0.7194 | 0.5827 | 0.7714 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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