Standard name
Human Ortholog
Description Subunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER; human homolog GPAA1 can complement growth defects of yeast thermosensitive mutant at restrictive temperature
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 – – – 0.07 0 0.1 0 0 0 0.07 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 – – – 0.14 0.07 0.18 0 0 0 0 0 0
Cytoplasm – 0.5 0.9 0.9 0.77 0.62 0.57 0.55 – 0.37 0.43 0.37 – – – 0.36 0.42 0.39 0 0.24 0.43 0.17 0.56 0.45
Endoplasmic Reticulum – 0.4 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0.68 0.48 0.12 0.36 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0.06 0 0 0 0.06 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0.22 0 0 0 0 0
Mitochondria – 0.12 0.06 0.23 0.27 0.6 0.65 0.57 – 0.81 0.7 0.8 – – – 0.22 0.11 0.11 0 0.07 0.24 0.15 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0.12 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.13 0 0 0 0 0 0 – 0 0 0 – – – 0.21 0.34 0.18 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 0 0 0 0 0 0 – 0 0 0 – – – 11 8 10 0 2 5 0 0 0
Bud – 0 1 1 1 2 2 1 – 8 8 7 – – – 3 5 1 0 1 4 0 0 0
Bud Neck – 0 2 0 0 0 0 2 – 2 0 0 – – – 0 0 0 0 1 1 0 0 0
Bud Site – 0 0 0 0 0 1 1 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 1 2 1 4 4 3 4 – 4 6 5 – – – 24 12 18 0 1 5 0 0 0
Cytoplasm – 51 152 128 96 150 138 192 – 93 88 82 – – – 61 76 39 0 29 71 0 3 0
Endoplasmic Reticulum – 41 5 0 1 0 0 2 – 3 1 3 – – – 1 6 3 4 59 20 1 0 0
Endosome – 2 0 0 1 2 1 1 – 0 1 0 – – – 7 7 3 0 1 3 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 – – – 1 0 4 1 5 0 0 0 0
Mitochondria – 12 10 33 34 145 158 199 – 202 142 175 – – – 37 20 11 0 9 40 0 0 0
Nucleus – 0 0 0 0 0 0 2 – 1 1 0 – – – 3 3 4 0 0 0 0 0 0
Nuclear Periphery – 1 0 0 0 1 1 0 – 2 1 2 – – – 5 3 3 0 2 0 0 0 0
Nucleolus – 0 0 0 0 2 0 2 – 1 1 2 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 1 0 0 0 2 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 2 0 0 3 2 8 – 4 3 3 – – – 0 3 0 0 2 3 0 0 0
Vac/Vac Membrane – 13 1 1 3 7 8 14 – 0 7 3 – – – 36 62 18 0 5 2 0 0 0
Unique Cell Count 103 168 143 125 243 244 349 249 204 219 168 180 100 7 125 167 4 6 2
Labelled Cell Count 122 175 165 140 316 314 430 320 259 282 189 205 114 7 125 167 4 6 2


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.2 3.7 3.7 4.2 2.9 2.7 3.3 – 2.9 3.1 3.1 – – – 7.2 7.6 8.8 – – –
Std Deviation (1e-4) – 1.9 1.4 2.0 3.5 1.4 1.0 1.5 – 1.4 2.0 1.3 – – – 1.3 1.5 1.6 – – –
Intensity Change (Log2) – – – -0.01 0.15 -0.36 -0.48 -0.19 – -0.39 -0.25 -0.3 – – – 0.94 1.02 1.23 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 – – – 3.4 0 0
Bud 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 – – – 4.5 2.6 5.1
Cytoplasm -0.3 -3.2 -6.5 -7.4 -8.0 – -10.8 -9.5 -10.6 – – – -10.3 -9.5 -9.0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 – – – 0 0.2 0
Endosome 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 – – – 4.2 1.7 1.5
Nucleus 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 – – – 6.1 8.2 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4089 1.846 2.2382 2.0989 2.1369 2.031 4.7562 3.9811 4.165 4.6278 3.0743 4.0177 3.8604 2.9659 3.8051 7.5789 3.1799 4.6708
Actin 0.0491 0.0068 0.0699 0.1224 0.0185 0.008 0.038 0.0006 0.0299 0.0127 0.0004 0.0095 0.0667 0.0236 0.0415 0.0106 0.2185 0.0619
Bud 0.0046 0.006 0.0015 0.0016 0.0018 0.0002 0.0008 0 0.0011 0.0008 0 0.0003 0.0114 0.0226 0.0332 0.0081 0.0097 0.0053
Bud Neck 0.0114 0.0117 0.0092 0.0035 0.001 0.0037 0.0009 0.0001 0.0008 0.0023 0.0002 0.0144 0.0148 0.0031 0.0004 0.0021 0.0014 0.0837
Bud Periphery 0.0036 0.0026 0.002 0.0013 0.0016 0.0004 0.0024 0.0001 0.0042 0.0022 0 0.0006 0.0343 0.0268 0.0206 0.0206 0.0243 0.0147
Bud Site 0.0635 0.1489 0.0505 0.0172 0.0051 0.0004 0.0022 0.0005 0.0049 0.0069 0.0001 0.0005 0.0335 0.1121 0.0012 0.0037 0.003 0.0031
Cell Periphery 0.0015 0.0026 0.002 0.0004 0.0017 0.0004 0.0018 0.0011 0.0026 0.0033 0.0001 0.0007 0.0043 0.0023 0.0016 0.0037 0.0037 0.0025
Cytoplasm 0.3535 0.1969 0.2413 0.1979 0.1741 0.2586 0.1837 0.274 0.3293 0.2674 0.5625 0.2485 0.1593 0.3019 0.226 0.4034 0.2466 0.2218
Cytoplasmic Foci 0.0099 0.0228 0.0184 0.0409 0.0504 0.0312 0.0723 0.0046 0.0094 0.0303 0.0036 0.0094 0.0059 0.0146 0.0042 0.1091 0.0082 0.0665
Eisosomes 0.0004 0.0005 0.0006 0.0003 0.0005 0.0002 0.0005 0.0002 0.0005 0.0007 0 0.0002 0.0004 0.0003 0.0002 0.0002 0.0005 0.0003
Endoplasmic Reticulum 0.1931 0.2244 0.2121 0.0382 0.0543 0.1447 0.1277 0.3196 0.3003 0.2974 0.1517 0.354 0.265 0.1826 0.2885 0.0991 0.0096 0.1413
Endosome 0.0081 0.0364 0.0279 0.0291 0.2069 0.0283 0.116 0.0061 0.0166 0.0507 0.0054 0.0315 0.0107 0.0035 0.0106 0.0185 0.0124 0.0046
Golgi 0.0029 0.0032 0.0152 0.0339 0.1189 0.0019 0.0221 0.0006 0.0031 0.0375 0.0002 0.0035 0.0018 0.0007 0.0062 0.0161 0.0109 0.001
Lipid Particles 0.003 0.0062 0.0084 0.0306 0.0136 0.0118 0.042 0.0047 0.0093 0.0691 0.0008 0.0042 0.0052 0.0063 0.0032 0.0104 0.002 0.0027
Mitochondria 0.0018 0.0025 0.005 0.0041 0.0476 0.0006 0.0276 0.0003 0.0047 0.037 0.0001 0.0098 0.0018 0.0048 0.0202 0.0037 0.0034 0.0832
None 0.2506 0.2911 0.301 0.4364 0.2704 0.4184 0.1904 0.3661 0.1874 0.1235 0.257 0.253 0.2912 0.2684 0.2888 0.1839 0.3985 0.2775
Nuclear Periphery 0.0097 0.0104 0.0132 0.0107 0.0031 0.0305 0.0715 0.011 0.0203 0.0099 0.0097 0.0419 0.0537 0.0086 0.032 0.005 0.0018 0.0052
Nucleolus 0.0018 0.0024 0.0017 0.0004 0.0004 0.0031 0.0012 0.0002 0.0045 0.0049 0 0.0002 0.0015 0.0026 0.0004 0.0017 0.0009 0.0011
Nucleus 0.0158 0.0092 0.0045 0.0108 0.0011 0.0242 0.0146 0.003 0.0312 0.0031 0.0054 0.0031 0.0214 0.0047 0.0089 0.007 0.0063 0.0055
Peroxisomes 0.0017 0.0017 0.0042 0.0137 0.0069 0.0012 0.0507 0.0001 0.0017 0.022 0 0.0019 0.0009 0.0005 0.0059 0.0325 0.0016 0.0071
Punctate Nuclear 0.0099 0.0073 0.0046 0.0041 0.0006 0.0269 0.014 0.001 0.0235 0.002 0.0008 0.0012 0.0022 0.0014 0.0015 0.009 0.0007 0.0077
Vacuole 0.0035 0.0053 0.005 0.0022 0.0128 0.0043 0.0122 0.0059 0.0132 0.0122 0.0019 0.0083 0.0112 0.0072 0.0038 0.0495 0.0349 0.0024
Vacuole Periphery 0.0005 0.001 0.0019 0.0004 0.0086 0.001 0.0074 0.0004 0.0012 0.004 0.0002 0.0031 0.003 0.0015 0.0009 0.002 0.0012 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.4616 26.1964 20.2323 31.9691 28.3021 22.7134 35.5415 35.7541 31.8991 46.2305
Translational Efficiency 0.8571 1.0598 1.1842 0.6775 0.9417 1.41 0.8247 0.5305 0.7093 0.7075

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
223 529 331 75 22 1277 1831 24 245 1806 2162 99

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 931.45 834.97 1061.11 1021.92 740.78 892.74 964.36 1042.04 914.33 875.82 979.17 1026.80
Standard Deviation 148.98 140.06 133.98 159.52 67.76 118.84 133.41 155.02 153.58 128.15 137.97 158.68
Intensity Change Log 2 — -0.157754 0.188024 0.133732 — 0.269195 0.380527 0.492294 — 0.047088 0.276483 0.303642

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002372 0.000776 0.000501 0.001724 0.004545 0.000678 0.001171 0.001908 0.002567 0.000706 0.001068 0.001769
Bud Neck 0.005052 0.002705 0.003624 0.008909 0.004445 0.002268 0.005503 0.011035 0.004997 0.002396 0.005215 0.009424
Bud Site 0.021812 0.005684 0.002039 0.064797 0.014163 0.005547 0.012855 0.017998 0.021125 0.005587 0.011199 0.053452
Cell Periphery 0.001240 0.000725 0.000381 0.000936 0.010855 0.001293 0.000610 0.000553 0.002103 0.001126 0.000575 0.000843
Cytoplasm 0.080693 0.113452* 0.056195 0.089141 0.312376* 0.113514* 0.135292* 0.194352* 0.101497* 0.113496* 0.123183* 0.114647*
Cytoplasmic Foci 0.039475 0.048121 0.006705 0.037335 0.136589 0.037318 0.016393 0.070333 0.048195 0.040482 0.014910 0.045334
Eisosomes 0.000500 0.000356 0.000047 0.000105 0.001521 0.000506 0.000115 0.000243 0.000592 0.000462 0.000105 0.000139
Endoplasmic Reticulum 0.490514* 0.599490* 0.318717* 0.326418* 0.294418* 0.657150* 0.438402* 0.146072* 0.472906* 0.640261* 0.420079* 0.282697*
Endosome 0.025216 0.017588 0.014361 0.034718 0.016001 0.010127 0.017704 0.033377 0.024388 0.012313 0.017192 0.034393
Golgi 0.011077 0.016641 0.000246 0.015255 0.019730 0.015538 0.001685 0.002126 0.011854 0.015861 0.001465 0.012072
Lipid Particles 0.028453 0.057610 0.010108 0.024112 0.075735 0.066463 0.015884 0.014338 0.032699 0.063869 0.014999 0.021742
Mitochondria 0.001237 0.010924 0.001389 0.009445 0.023991 0.009051 0.002589 0.001884 0.003280 0.009599 0.002405 0.007612
Mitotic Spindle 0.006038 0.004971 0.003708 0.004003 0.000096 0.002001 0.006544 0.007952 0.005504 0.002871 0.006110 0.004960
None 0.000113 0.002699 0.002073 0.000250 0.001083 0.001584 0.002088 0.000432 0.000200 0.001911 0.002085 0.000294
Nuclear Periphery 0.036180 0.018406 0.289705* 0.058057 0.016178 0.018211 0.125824* 0.015987 0.034384 0.018268 0.150914* 0.047858
Nuclear Periphery Foci 0.053525 0.044830 0.140789 0.126470 0.014814 0.020721 0.079694 0.085685 0.050048 0.027783 0.089048 0.116582
Nucleolus 0.000188 0.000858 0.006203 0.000859 0.000188 0.000512 0.000965 0.002757 0.000188 0.000613 0.001767 0.001319
Nucleus 0.025076 0.020358 0.094728 0.036451 0.018325 0.016190 0.058195 0.027306 0.024470 0.017411 0.063788 0.034234
Peroxisomes 0.001024 0.003268 0.000403 0.001396 0.014433 0.003233 0.000753 0.029537 0.002228 0.003243 0.000700 0.008218
Vacuole 0.163501 0.025690 0.046622 0.154224 0.018271 0.015454 0.073562 0.324390* 0.150460 0.018452 0.069438 0.195476
Vacuole Periphery 0.006716 0.004849 0.001456 0.005397 0.002243 0.002643 0.004172 0.011734 0.006314 0.003289 0.003756 0.006933

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.51 2.92 0.78 -2.05 -2.60 1.13 0.95 0.79 -1.49 -0.65 2.87 2.06 1.06 -2.62 -1.34
Bud Neck 1.80 0.69 -1.90 -3.07 -2.46 0.98 -0.72 -0.90 -1.62 -0.60 2.28 -0.60 -2.03 -3.62 -1.91
Bud Site 2.77 3.44 -1.65 -2.54 -2.71 1.39 0.15 -0.14 -1.94 -0.40 2.97 1.88 -1.55 -2.67 -2.30
Cell Periphery 1.65 3.03 1.09 -0.58 -2.36 1.16 1.24 1.26 2.26 0.66 1.16 1.90 1.63 1.31 -1.18
Cytoplasm -2.08 2.20 -0.21 1.00 -1.46 3.25 2.78 1.22 -1.76 -1.24 -0.53 -1.87 -0.57 -0.33 0.42
Cytoplasmic Foci -1.20 6.13 -0.10 0.67 -3.47 2.22 2.68 1.60 -1.18 -2.31 1.20 5.14 0.30 -0.56 -3.49
Eisosomes 0.95 3.82 3.19 2.91 -3.91 1.31 1.83 1.68 2.29 -1.83 0.81 3.74 3.43 3.94 -1.50
Endoplasmic Reticulum -4.45 5.00 4.40 8.17 1.17 -4.68 -1.61 2.16 13.47 7.70 -7.35 1.78 5.66 12.93 5.58
Endosome 2.04 3.13 -0.95 -2.20 -2.77 1.59 -0.41 -0.77 -1.32 -0.67 4.19 2.40 -0.96 -3.33 -2.38
Golgi -1.30 2.42 -0.17 0.68 -1.68 0.40 1.77 1.78 7.91 0.07 -1.12 2.51 0.30 1.24 -1.52
Lipid Particles -5.31 4.92 0.79 5.25 -2.67 0.55 3.67 3.73 9.94 0.66 -7.25 4.90 2.27 9.32 -1.54
Mitochondria -4.18 -0.47 -1.41 0.74 -1.36 1.04 1.50 1.59 4.23 1.29 -3.35 0.58 -0.74 1.06 -1.05
Mitotic Spindle 0.32 0.52 0.64 0.40 0.03 -2.56 -5.70 -1.28 -0.97 -0.23 0.88 -0.15 0.22 -1.02 0.61
None -3.48 -2.35 -1.56 3.21 2.12 -0.89 -1.30 1.22 3.05 2.65 -5.33 -3.65 -1.16 4.81 3.36
Nuclear Periphery 2.27 -13.96 -0.70 -1.73 10.33 -0.21 -9.49 0.40 1.05 13.69 2.34 -14.39 -0.38 -1.59 8.21
Nuclear Periphery Foci 0.85 -7.70 -2.98 -3.45 1.53 -0.66 -6.69 -1.66 -1.53 0.27 3.26 -5.73 -3.03 -4.55 -0.74
Nucleolus -2.93 -3.47 -1.31 0.02 2.95 -1.45 -2.49 -1.14 -0.90 -0.54 -2.40 -4.14 -1.74 -0.86 1.10
Nucleus 1.18 -6.75 -0.78 -1.38 4.50 0.46 -9.05 -1.28 -1.40 0.79 1.98 -8.76 -1.19 -2.16 3.15
Peroxisomes -5.13 3.11 -0.97 3.32 -2.42 1.16 1.44 -0.26 -0.90 -1.05 -1.18 1.68 -0.81 -0.56 -1.16
Vacuole 8.87 7.07 -1.90 -6.87 -5.99 0.59 -11.42 -5.29 -5.34 -4.31 9.21 5.33 -3.38 -8.76 -6.69
Vacuole Periphery 0.50 1.76 0.63 0.30 -1.52 -0.45 -1.64 -0.79 -0.74 -0.55 1.05 0.92 0.25 -0.82 -0.67
Standard name
Human Ortholog
Description Subunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER; human homolog GPAA1 can complement growth defects of yeast thermosensitive mutant at restrictive temperature
Localization
Cell Percentages ER (73%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gaa1

Gaa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gaa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available