Standard name
Human Ortholog
Description DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.15 0.27 0.35 0.51 0.62 0.71 – 0.64 0.69 0.49 0.62 0.45 0.49 0.65 0.7 0.72 0.67 0 0.05 0.09 0.23 0.24 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0.11 0.08 0.06 0 – 0 0 0 0 0.05 0 0 0.05 0.06 0.08 0 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.06 0 0.16 0 0.24 0.21 – 0.39 0.26 0.59 0.43 0 0 0 0.07 0 0 0 0 0.06 0.05 0.07 0.07
Nucleus 0.67 0.53 0.48 0.26 0.09 0.06 0 – 0 0 0 0 0.42 0.39 0.29 0 0 0 0.57 0.5 0.35 0.26 0.22 0.17
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.06 0 0.05 – 0 0 0 0.05 0 0 0 0 0 0 0.06 0.06 0.06 0.1 0.07 0.08
Vac/Vac Membrane 0.14 0.29 0.23 0.14 0.27 0.08 0.08 – 0 0.05 0 0 0.19 0.16 0.14 0.3 0.27 0.34 0.21 0.19 0.32 0.22 0.3 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 4 1 0 0 1
Bud 0 1 0 3 0 0 3 – 6 2 7 6 0 1 0 0 0 0 7 9 6 3 5 6
Bud Neck 0 0 6 2 5 4 7 – 3 6 1 1 1 1 0 0 1 1 1 3 4 0 3 4
Bud Site 0 0 0 0 0 1 0 – 3 0 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 – 0 3 0 0 0 0 0 3 0 4 0 0 0 0 0 0
Cytoplasm 44 71 69 67 99 167 259 – 199 180 164 203 33 116 123 227 157 173 26 32 38 27 70 53
Endoplasmic Reticulum 0 4 0 2 3 0 0 – 1 1 2 2 0 2 0 19 9 17 1 0 2 0 1 2
Endosome 7 18 7 21 16 16 7 – 3 5 2 3 4 3 2 17 12 20 12 33 7 2 7 8
Golgi 1 2 1 1 0 0 0 – 0 0 0 0 0 0 1 4 2 1 2 1 1 0 0 1
Mitochondria 106 29 3 31 8 65 75 – 120 67 200 142 0 2 0 22 4 5 10 25 25 5 19 20
Nucleus 319 245 121 51 18 15 15 – 10 5 13 6 31 93 55 5 7 5 352 300 142 31 66 48
Nuclear Periphery 0 0 1 0 0 0 0 – 0 0 3 0 0 0 0 2 0 0 2 6 1 0 0 3
Nucleolus 1 2 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 11 15 4 3 4 2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 5 4 4 12 12 8 19 – 8 7 8 15 1 4 1 0 3 0 37 37 23 12 19 20
Vac/Vac Membrane 66 135 59 27 53 22 29 – 12 12 12 11 14 38 26 98 58 88 132 117 131 26 87 92
Unique Cell Count 477 464 253 194 194 270 365 310 259 338 329 74 239 190 326 218 257 618 602 407 121 296 275
Labelled Cell Count 549 512 273 217 214 298 416 366 289 416 391 84 260 208 397 253 314 618 602 407 121 296 275


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.0 5.7 5.1 5.3 4.4 4.3 – 3.8 4.0 3.4 3.8 5.5 5.4 5.3 6.7 7.0 6.7 4.9 5.2 5.6
Std Deviation (1e-4) 0.6 0.9 1.2 1.2 1.3 1.3 0.9 – 0.9 0.9 1.2 1.2 1.1 0.9 1.3 2.2 2.7 2.3 0.7 1.2 1.3
Intensity Change (Log2) – – – -0.14 -0.11 -0.38 -0.41 – -0.57 -0.51 -0.74 -0.59 -0.05 -0.07 -0.1 0.24 0.31 0.24 -0.21 -0.12 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 5.1 7.9 10.7 – 8.7 9.6 5.2 8.3 2.8 4.9 7.9 10.1 9.7 9.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.9 0 4.2
Endosome 3.5 2.6 0 0 – 0 0 0 0 0 0 0 1.5 1.5 2.5
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.8 0 0 0 – 0 0 0 0 0 0 0 3.3 0 0
Nucleus -4.6 -8.7 -11.0 -12.9 – -12.5 -12.1 -12.6 -13.3 -0.9 -2.0 -4.0 -13.4 -10.9 -12.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.6 2.6 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.5 1.0 -4.8 -5.4 – -6.9 -6.1 -7.3 -7.3 -0.8 -2.1 -2.5 1.8 0.8 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3145 -0.7381 -1.0702 -0.7593 -0.8597 -1.0636 -0.2203 0.0104 0.2864 -0.1208 -0.4756 0.32 -0.0594 -0.1595 -0.5688 -0.388 -0.3417 -0.4833
Actin 0.0355 0.001 0.0131 0.0495 0.0804 0.0019 0.0125 0.0003 0.0075 0.001 0.0066 0.0108 0.0234 0.0004 0.018 0.01 0.0115 0.0039
Bud 0.0006 0.0012 0.0004 0.0005 0.0021 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0067 0.0005 0.0002 0.0002 0.0005
Bud Neck 0.0036 0.0004 0.0009 0.0009 0.0013 0.002 0.0012 0.0037 0.0024 0.0188 0.0014 0.0059 0.0016 0.0004 0.0024 0.0015 0.0009 0.004
Bud Periphery 0.001 0.0041 0.0008 0.0011 0.0039 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0004 0.0005 0.001 0.0007 0.0002 0.0002 0.0003
Bud Site 0.0103 0.0111 0.0023 0.0057 0.0026 0.0007 0.0012 0.0139 0.0026 0.0039 0.0003 0.0006 0.0014 0.0047 0.0023 0.0005 0.0005 0.0004
Cell Periphery 0.0006 0.0003 0.0002 0.0008 0.0003 0.0001 0.0003 0.0001 0.0014 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.1666 0.1946 0.176 0.1178 0.0348 0.1763 0.1178 0.1149 0.091 0.0685 0.0828 0.1262 0.1539 0.1958 0.1873 0.2052 0.1594 0.2241
Cytoplasmic Foci 0.0233 0.0113 0.0295 0.0441 0.0474 0.0196 0.0161 0.0051 0.0129 0.0108 0.0109 0.0071 0.0173 0.0148 0.0089 0.0323 0.0071 0.0152
Eisosomes 0.0005 0.0003 0.0003 0.0031 0.0005 0.0002 0.0003 0.0001 0.001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0055 0.0091 0.0076 0.005 0.002 0.0029 0.0043 0.0019 0.006 0.0019 0.0035 0.0049 0.0107 0.0044 0.0027 0.0062 0.0051 0.0042
Endosome 0.0208 0.009 0.0277 0.018 0.0931 0.0151 0.0072 0.002 0.0049 0.0037 0.0168 0.0069 0.0112 0.0039 0.0032 0.0169 0.0173 0.0081
Golgi 0.0046 0.0011 0.0111 0.0095 0.0643 0.009 0.0013 0.0001 0.0013 0.0003 0.002 0.0021 0.0021 0.0002 0.0006 0.0034 0.0024 0.0006
Lipid Particles 0.0104 0.0098 0.0154 0.0648 0.0534 0.0151 0.0033 0.0006 0.0035 0.0015 0.0067 0.005 0.0059 0.0007 0.0013 0.0049 0.0029 0.0008
Mitochondria 0.0346 0.0025 0.0178 0.0838 0.0999 0.0159 0.001 0.0005 0.0014 0.0004 0.001 0.0076 0.0022 0.001 0.001 0.0011 0.0016 0.0013
None 0.5504 0.5725 0.4015 0.414 0.3955 0.5003 0.4163 0.4903 0.4716 0.4423 0.4723 0.3942 0.338 0.5476 0.3869 0.2414 0.5165 0.3088
Nuclear Periphery 0.009 0.016 0.0252 0.0206 0.0047 0.0089 0.0192 0.0103 0.0164 0.0183 0.0269 0.0145 0.039 0.0116 0.0113 0.0377 0.0335 0.0224
Nucleolus 0.0068 0.002 0.0049 0.0022 0.0029 0.0027 0.0058 0.0053 0.007 0.0064 0.0059 0.0068 0.0031 0.0022 0.0038 0.0027 0.0027 0.0062
Nucleus 0.0861 0.1247 0.1964 0.0827 0.0397 0.1478 0.3003 0.2732 0.2642 0.2815 0.2723 0.3153 0.3203 0.1461 0.3178 0.3397 0.2009 0.3126
Peroxisomes 0.0034 0.0025 0.006 0.0333 0.0252 0.0042 0.0025 0.0004 0.0028 0.0003 0.0014 0.0029 0.0068 0.0004 0.0038 0.0011 0.0006 0.0009
Punctate Nuclear 0.0195 0.0176 0.0574 0.036 0.0409 0.0746 0.0869 0.0755 0.0993 0.1391 0.087 0.0854 0.0556 0.0552 0.0449 0.0923 0.0336 0.0819
Vacuole 0.0059 0.0063 0.0039 0.0034 0.0025 0.0018 0.0017 0.0015 0.0016 0.0006 0.0013 0.0021 0.0041 0.0024 0.0017 0.0018 0.0018 0.0025
Vacuole Periphery 0.001 0.0027 0.0015 0.0032 0.0024 0.0006 0.0004 0.0002 0.0007 0.0003 0.0007 0.0006 0.0019 0.0004 0.0004 0.0007 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1005 9.6257 7.5186 7.7645 7.3478 11.3281 8.9641 11.7546 9.1135 8.828
Translational Efficiency 0.4711 0.2941 0.3659 0.3483 0.4043 0.3032 0.4548 0.2713 0.3503 0.4419

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
Localization
Cell Percentages nucleus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cha4

Cha4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cha4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available