ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0 0 0 0 0.08 0.09 0.11 0 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.1 0 0 0.07 0.12 0.08 0.13 0.18 0.12 0.18 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.95 0.95 0.85 0.92 0.92 0.85 0.84 0.83 0.82 0.8 0.84 0.78 0.86 0.85 0.78 0.81 0.83 0.87 0.93 0.94 0.88 0.89 0.64 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0.08 0.05 0.07 0.15 0.19 0.18 0.27 0.22 0.24 0.26 0.15 0.06 0.07 0.08 0.08 0.07 0 0 0 0 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 0 0 0 1 1 2 2 0 0 0 0 0 0 0 1 0 1 1 1 7
Bud Neck 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 1 0 0 3 5 4 9 14 4 10 1 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 6 5 9 14 6 9 5 12 1 5 6 27 29 6 11 4 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 2 1 0 0 0 0 0 0
Endosome 0 0 3 0 0 0 0 0 0 0 0 0 0 9 8 2 2 1 3 0 6 0 9 23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 7
Mitochondria 13 5 20 1 2 18 40 36 41 64 33 56 1 1 0 9 5 2 1 0 1 0 2 8
Nucleus 274 92 168 144 208 234 290 355 259 283 237 236 61 251 209 118 119 85 266 106 176 132 130 69
Nuclear Periphery 1 0 0 1 0 5 13 9 4 4 5 1 0 1 1 2 4 0 0 0 0 0 0 0
Nucleolus 16 2 15 8 16 40 67 76 85 76 69 78 11 19 19 11 11 7 2 0 2 3 15 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 8 1 0 1 0 0 0 0 1 8
SpindlePole 0 0 1 0 0 0 1 1 0 0 0 0 1 6 11 3 3 1 0 0 1 0 11 43
Vac/Vac Membrane 3 1 3 2 3 2 3 2 3 2 1 2 0 2 3 11 2 2 6 2 8 3 22 28
Unique Cell Count 289 97 197 156 225 274 344 430 317 352 282 303 71 296 267 145 143 98 286 114 202 149 204 216
Labelled Cell Count 307 102 217 162 238 316 426 494 408 457 352 388 81 319 292 163 159 104 286 114 202 149 204 216


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 9.5 9.1 11.4 11.4 9.3 7.7 7.5 7.2 6.9 7.0 6.7 17.1 14.8 11.4 12.0 14.1 15.9 9.8 8.7 7.8
Std Deviation (1e-4) 2.1 2.5 3.1 3.4 3.7 2.7 3.2 2.4 2.2 2.1 2.0 2.1 6.9 6.6 5.3 3.7 4.8 5.3 2.7 2.3 1.9
Intensity Change (Log2) – – – 0.33 0.32 0.03 -0.24 -0.28 -0.34 -0.4 -0.37 -0.44 0.91 0.7 0.33 0.4 0.63 0.8 0.11 -0.07 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.5 1.1 0 0 0 0.2 0 0 0 2.6 3.2 0.5 1.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.8 -4.3 -1.4 0.5 -0.7 0.9 2.5 0.5 2.5 -2.4 -5.3 -5.3 -1.3 -2.3 -2.5
Nucleus 2.0 2.4 0 -0.3 -0.9 -1.1 -1.4 -0.4 -2.1 0.1 -0.1 -1.9 -1.0 -0.5 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 -0.2 2.3 3.7 3.3 5.3 4.2 4.8 5.1 1.9 -0.5 -0.2 0 0 -0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.7355 17.4019 15.1395 17.1796 13.3766 15.6342 12.4153 16.3887 15.1293 16.9588 16.4326 16.5656 16.4953 20.3872 16.9315 18.3331 15.204 18.4392
Actin 0.0215 0 0.0002 0 0.0186 0.0001 0.017 0 0.0002 0 0.0155 0.0001 0.0161 0 0.019 0 0.0035 0
Bud 0.0002 0 0 0 0.0007 0.0002 0.0002 0 0 0 0.001 0 0.0004 0.0001 0.0002 0 0.0003 0
Bud Neck 0.0006 0 0 0.0002 0.0006 0.0004 0.0002 0 0.0001 0 0.0002 0.0005 0.0003 0 0.0001 0 0.0002 0.0002
Bud Periphery 0.0003 0 0 0 0.0011 0.0005 0.0003 0 0.0001 0 0.0025 0.0001 0.0004 0 0.0002 0 0.0009 0.0001
Bud Site 0.0013 0 0 0 0.0005 0.0001 0.0004 0 0.0001 0 0.0012 0 0.0006 0 0.0004 0 0.0006 0
Cell Periphery 0.0002 0 0 0 0.0001 0.0005 0.0001 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0008 0
Cytoplasm 0.001 0 0.0006 0 0.0033 0.0007 0.0007 0 0.0018 0 0.0002 0.0002 0.0024 0.0001 0.0003 0.0001 0.0005 0
Cytoplasmic Foci 0.0139 0 0.0021 0 0.0018 0 0.0128 0 0.0008 0 0.0009 0 0.0271 0.0001 0.0097 0 0.0091 0
Eisosomes 0.0005 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0002 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0036 0 0.0002 0 0.0071 0.0003 0.0025 0 0.0003 0 0.0007 0.0001 0.0041 0 0.0001 0 0.0004 0
Endosome 0.0057 0 0.0039 0 0.0352 0 0.0039 0 0.0005 0 0.0035 0.0001 0.0105 0 0.001 0 0.0038 0
Golgi 0.0023 0 0.0002 0 0.0074 0 0.002 0 0 0 0.0021 0 0.0067 0 0.0014 0 0.0035 0
Lipid Particles 0.0066 0 0.0026 0 0.0055 0 0.0031 0 0.0004 0 0.002 0 0.0203 0 0.0083 0 0.0144 0
Mitochondria 0.0058 0.0002 0.0027 0.0001 0.008 0.0003 0.0037 0.0001 0.0011 0.0005 0.0265 0.0003 0.0019 0.0011 0.0042 0.0005 0.0722 0.0002
None 0.0015 0 0.0013 0 0.0027 0 0.002 0 0.0005 0 0.0006 0 0.0019 0 0.0009 0.0001 0.0011 0
Nuclear Periphery 0.0025 0.0029 0.003 0.0003 0.0885 0.0006 0.0149 0.0005 0.0065 0.0003 0.0084 0.0089 0.0027 0.0035 0.0013 0.0007 0.0021 0.0001
Nucleolus 0.0465 0.0231 0.0147 0.0339 0.0636 0.0743 0.0802 0.0115 0.0251 0.0098 0.15 0.0558 0.0335 0.0241 0.0298 0.0326 0.1404 0.0496
Nucleus 0.8693 0.9731 0.9657 0.965 0.7406 0.9196 0.8292 0.9878 0.947 0.9893 0.7789 0.9279 0.8332 0.9674 0.9082 0.9648 0.7267 0.9495
Peroxisomes 0.0042 0 0.0004 0 0.0005 0 0.0026 0 0 0 0.0014 0 0.0227 0 0.0108 0 0.0068 0
Punctate Nuclear 0.0118 0.0005 0.0012 0.0004 0.0055 0.0001 0.0219 0 0.0139 0 0.0022 0.0045 0.0121 0.0001 0.0026 0.0003 0.0059 0
Vacuole 0.0005 0 0.0002 0.0001 0.0029 0.0018 0.0007 0 0.0004 0 0.0005 0.0005 0.0017 0.001 0.0004 0.0002 0.0027 0.0001
Vacuole Periphery 0.0005 0.0001 0.0009 0 0.0057 0.0005 0.0015 0 0.0012 0 0.0014 0.0007 0.001 0.0022 0.0008 0.0003 0.0038 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.7316 29.7672 27.1087 38.449 13.2464 53.7026 50.7896 52.8621 43.0171 27.4804
Translational Efficiency 0.5227 0.8551 0.7594 0.5635 0.696 0.4857 0.6278 0.53 0.5609 0.5776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2086 1269 529 1468 1494 876 128 136 3580 2145 657 1604

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 750.44 915.40 1252.61 1461.65 873.32 1002.90 1320.23 1795.05 801.72 951.13 1265.78 1489.92
Standard Deviation 133.91 216.89 199.94 354.46 138.62 204.03 182.83 407.17 148.79 216.05 198.54 371.04
Intensity Change Log 2 — 0.286666 0.739129 0.961789 — 0.199595 0.596207 1.039442 — 0.240490 0.664023 1.004072

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000360 0.000612 0.000741 0.001020 0.000289 0.000728 0.001179 0.001045 0.000331 0.000659 0.000827 0.001022
Bud Neck 0.004924 0.006023 0.012708 0.022266 0.004657 0.005605 0.010101 0.019069 0.004813 0.005852 0.012200 0.021995
Bud Site 0.001750 0.001100 0.000938 0.004249 0.000715 0.000913 0.001088 0.002481 0.001318 0.001024 0.000967 0.004099
Cell Periphery 0.000665 0.000523 0.000043 0.000188 0.000421 0.000145 0.000046 0.000099 0.000563 0.000369 0.000043 0.000180
Cytoplasm 0.003903 0.003286 0.000411 0.000886 0.005342 0.000703 0.000247 0.000676 0.004504 0.002231 0.000379 0.000868
Cytoplasmic Foci 0.001148 0.000637 0.000031 0.003694 0.000355 0.001056 0.000078 0.005449 0.000817 0.000808 0.000040 0.003842
Eisosomes 0.000039 0.000035 0.000042 0.000057 0.000028 0.000039 0.000056 0.000084 0.000034 0.000037 0.000045 0.000059
Endoplasmic Reticulum 0.007425 0.006105 0.002723 0.000949 0.005637 0.004004 0.004274 0.000551 0.006678 0.005247 0.003025 0.000915
Endosome 0.000656 0.000640 0.000069 0.001797 0.000344 0.000555 0.000101 0.000083 0.000526 0.000605 0.000075 0.001652
Golgi 0.000077 0.000294 0.000046 0.001246 0.000039 0.000459 0.000074 0.000494 0.000061 0.000361 0.000052 0.001183
Lipid Particles 0.000099 0.000342 0.000057 0.000369 0.000124 0.001487 0.000089 0.000071 0.000110 0.000810 0.000063 0.000344
Mitochondria 0.001085 0.001410 0.001152 0.004644 0.000472 0.001895 0.001512 0.003226 0.000829 0.001608 0.001222 0.004524
Mitotic Spindle 0.000176 0.003023 0.000503 0.031634 0.001187 0.003631 0.000497 0.040736 0.000598 0.003271 0.000501 0.032406
None 0.012027 0.013355 0.000670 0.001792 0.006703 0.001672 0.000896 0.002040 0.009805 0.008583 0.000714 0.001813
Nuclear Periphery 0.000318 0.000334 0.000197 0.001145 0.000337 0.000369 0.000289 0.001442 0.000326 0.000348 0.000215 0.001170
Nuclear Periphery Foci 0.000307 0.000887 0.000035 0.000910 0.000166 0.002726 0.000041 0.001469 0.000248 0.001638 0.000036 0.000958
Nucleolus 0.012836 0.012162 0.005670 0.114629 0.008337 0.010504 0.016534 0.139815 0.010958 0.011485 0.007786 0.116765
Nucleus 0.950161* 0.947467* 0.972511* 0.797364* 0.963368* 0.960634* 0.960118* 0.760393* 0.955672* 0.952844* 0.970097* 0.794230*
Peroxisomes 0.000259 0.000413 0.000367 0.007763 0.000150 0.001152 0.000732 0.019649 0.000214 0.000715 0.000438 0.008770
Vacuole 0.001319 0.000971 0.001002 0.001979 0.001133 0.001019 0.001942 0.000676 0.001241 0.000990 0.001185 0.001868
Vacuole Periphery 0.000464 0.000380 0.000084 0.001420 0.000199 0.000704 0.000107 0.000451 0.000353 0.000512 0.000088 0.001338

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.23 -9.06 -15.34 -8.77 -4.56 -3.68 -6.61 -9.02 -2.98 1.89 -5.92 -11.13 -17.80 -6.50 -1.94
Bud Neck -1.69 -8.12 -13.42 -12.58 -7.41 -1.22 -4.42 -5.58 -5.20 -1.26 -2.09 -9.45 -14.62 -13.59 -7.02
Bud Site 1.42 1.96 -5.84 -7.33 -8.00 -1.08 -2.66 -4.77 -4.45 -0.47 0.98 -1.03 -7.57 -8.26 -6.62
Cell Periphery 0.81 7.37 5.59 2.33 -4.16 1.99 2.55 2.33 1.01 -1.01 1.65 6.89 5.09 2.22 -4.08
Cytoplasm 0.51 4.54 3.90 2.88 -2.72 4.29 4.78 4.33 0.07 -1.32 2.78 6.55 5.73 2.68 -2.98
Cytoplasmic Foci 1.13 2.40 -3.77 -4.93 -5.85 -1.74 -0.57 -3.06 -2.80 -2.90 0.23 2.24 -5.16 -5.40 -6.32
Eisosomes 1.16 -1.71 -6.78 -7.63 -5.36 -4.11 -7.52 -5.92 -4.98 -2.29 -1.50 -6.06 -9.68 -8.66 -4.91
Endoplasmic Reticulum 2.43 9.11 16.68 14.00 7.09 3.35 0.46 13.09 10.50 6.32 3.78 8.31 20.82 17.00 9.14
Endosome 0.07 4.76 -1.67 -1.47 -3.60 -1.46 1.24 3.45 2.94 1.69 -0.49 5.50 -2.02 -1.58 -3.50
Golgi -2.03 1.33 -3.63 -2.63 -3.70 -2.41 -3.05 -6.19 -1.34 -3.57 -3.16 -2.17 -3.92 -2.51 -3.77
Lipid Particles -1.53 1.03 -1.33 0.38 -1.65 -1.84 -0.91 0.38 1.85 1.13 -2.19 0.93 -1.28 1.66 -1.50
Mitochondria -0.61 -0.41 -5.58 -5.49 -7.79 -1.82 -8.98 -6.66 -2.04 -3.41 -1.73 -2.26 -7.91 -5.01 -7.91
Mitotic Spindle -2.37 -1.11 -10.82 -9.45 -9.87 -1.37 -1.47 -3.53 -3.35 -3.05 -2.65 -1.45 -11.17 -10.11 -10.10
None -0.53 9.01 7.98 6.04 -8.40 4.62 5.23 4.29 -0.65 -2.05 0.92 10.55 9.11 5.84 -8.20
Nuclear Periphery -0.57 1.36 -4.88 -4.63 -5.24 -0.21 -1.87 -1.56 -1.37 -1.17 -0.40 0.54 -5.10 -4.32 -5.16
Nuclear Periphery Foci -1.71 2.01 -3.55 -0.11 -4.45 -2.25 -0.85 -1.63 1.49 -1.32 -2.73 1.26 -4.20 1.40 -4.55
Nucleolus 0.10 1.98 -24.71* -24.30* -24.42* -1.73 -3.32 -9.40 -9.26 -7.41 -0.93 -1.50 -26.64* -26.20* -24.37*
Nucleus 0.74 -4.07 25.89 24.42 27.10 0.78 3.48 10.77 10.56 8.15 1.10 0.03 28.73 27.39 26.62
Peroxisomes -1.34 -1.74 -13.05 -12.84 -12.91 -3.35 -2.57 -4.63 -4.49 -4.32 -3.60 -3.18 -12.92 -12.31 -12.23
Vacuole 0.68 0.08 -1.29 -1.85 -1.50 0.17 -1.89 -0.51 -0.75 1.75 0.60 -1.49 -1.54 -2.05 0.00
Vacuole Periphery 0.37 3.13 -2.02 -2.07 -3.54 -1.10 0.44 -4.07 0.40 -4.54 -0.70 3.43 -2.56 -1.53 -3.62
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

YLR108C

YLR108C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr108c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available