| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 0 | 1 | 1 | 4 | 1 |
| Bud | 1 | 0 | – | – | – | – | – | 0 | – | – | – | – | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 2 | 5 | 3 |
| Bud Neck | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 4 | 0 | – | – | – | – | – | 1 | – | – | – | – | 1 | 13 | 21 | 11 | 14 | 11 | 0 | 0 | 0 | 0 | 1 | 0 |
| Cytoplasm | 51 | 10 | – | – | – | – | – | 21 | – | – | – | – | 15 | 26 | 38 | 86 | 111 | 99 | 2 | 6 | 1 | 3 | 9 | 8 |
| Endoplasmic Reticulum | 106 | 13 | – | – | – | – | – | 5 | – | – | – | – | 44 | 70 | 96 | 2 | 1 | 0 | 128 | 38 | 33 | 50 | 69 | 81 |
| Endosome | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 1 | 6 | 3 | 0 | 1 | 0 | 0 | 1 | 0 |
| Golgi | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 2 | 1 | 7 | 17 | 13 | 0 | 0 | 0 | 0 | 2 | 1 |
| Mitochondria | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 0 |
| Nucleus | 0 | 0 | – | – | – | – | – | 2 | – | – | – | – | 0 | 0 | 0 | 6 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | – | – | – | – | – | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | 1 | 0 | – | – | – | – | – | 0 | – | – | – | – | 3 | 5 | 8 | 68 | 112 | 72 | 3 | 7 | 2 | 1 | 5 | 5 |
| Unique Cell Count | 136 | 22 | 27 | 62 | 105 | 150 | 170 | 244 | 187 | 143 | 64 | 41 | 62 | 109 | 107 | |||||||||
| Labelled Cell Count | 163 | 23 | 29 | 65 | 117 | 166 | 185 | 273 | 207 | 143 | 64 | 41 | 62 | 109 | 107 | |||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 38.5 | 30.8 | – | – | – | – | – | 17.4 | – | – | – | – | 32.2 | 32.3 | 27.9 | 24.0 | 25.2 | 23.1 | 32.4 | 29.7 | 29.7 |
| Std Deviation (1e-4) | 11.8 | 14.1 | – | – | – | – | – | 4.7 | – | – | – | – | 13.3 | 13.0 | 11.0 | 10.7 | 10.4 | 10.1 | 14.7 | 15.1 | 13.4 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.0266 | 0.0086 | 0.0208 | 0 | 0.001 | 0.0073 | 0.0029 | 0.0052 | 0.0172 | 0 | 0.0222 | 0.0303 | – | – | – | – | – | – |
| Bud | 0.0023 | 0.0022 | 0.0076 | 0 | 0.0004 | 0.0006 | 0.0033 | 0.0793 | 0.0451 | 0 | 0.0075 | 0.0018 | – | – | – | – | – | – |
| Bud Neck | 0.002 | 0.0018 | 0.005 | 0.0006 | 0.0002 | 0.0302 | 0.0004 | 0.0012 | 0.0016 | 0 | 0.0103 | 0.0024 | – | – | – | – | – | – |
| Bud Periphery | 0.0033 | 0.0016 | 0.0117 | 0 | 0.0001 | 0.0006 | 0.0157 | 0.0407 | 0.0239 | 0 | 0.019 | 0.0017 | – | – | – | – | – | – |
| Bud Site | 0.0041 | 0.0092 | 0.0038 | 0 | 0.0005 | 0.0001 | 0.006 | 0.1362 | 0.0056 | 0 | 0.0635 | 0.0008 | – | – | – | – | – | – |
| Cell Periphery | 0.006 | 0.0703 | 0.009 | 0 | 0.0006 | 0.0106 | 0.0052 | 0.0156 | 0.0057 | 0 | 0.0344 | 0.0021 | – | – | – | – | – | – |
| Cytoplasm | 0.1117 | 0.4867 | 0.1643 | 0.9963 | 0.0216 | 0.3965 | 0.0194 | 0.1106 | 0.1423 | 0.0025 | 0.0564 | 0.0473 | – | – | – | – | – | – |
| Cytoplasmic Foci | 0.0905 | 0.0131 | 0.0715 | 0.0001 | 0.7186 | 0.0013 | 0.0572 | 0.0711 | 0.0473 | 0.0001 | 0.1598 | 0.1165 | – | – | – | – | – | – |
| Eisosomes | 0.0133 | 0.0519 | 0.0689 | 0 | 0.0015 | 0.0168 | 0.002 | 0.0042 | 0.0012 | 0 | 0.0016 | 0.0005 | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0.0426 | 0.0872 | 0.0475 | 0.0001 | 0.0004 | 0.0809 | 0.0022 | 0.0021 | 0.0848 | 0 | 0.0054 | 0.0298 | – | – | – | – | – | – |
| Endosome | 0.0901 | 0.0006 | 0.0129 | 0 | 0.001 | 0.0045 | 0.0079 | 0.0029 | 0.0123 | 0 | 0.0433 | 0.0042 | – | – | – | – | – | – |
| Golgi | 0.0118 | 0.0015 | 0.003 | 0 | 0.0015 | 0.0028 | 0.0011 | 0.0003 | 0.0012 | 0 | 0.0407 | 0.0111 | – | – | – | – | – | – |
| Lipid Particles | 0.0271 | 0.0086 | 0.0119 | 0 | 0.0297 | 0.013 | 0.0024 | 0.0071 | 0.0158 | 0 | 0.0221 | 0.007 | – | – | – | – | – | – |
| Mitochondria | 0.0957 | 0.0107 | 0.0572 | 0 | 0.0109 | 0.001 | 0.0465 | 0.0806 | 0.015 | 0 | 0.0317 | 0.0095 | – | – | – | – | – | – |
| None | 0.1269 | 0.0288 | 0.154 | 0.0003 | 0.0813 | 0.0051 | 0.5511 | 0.3177 | 0.5083 | 0.9974 | 0.3285 | 0.464 | – | – | – | – | – | – |
| Nuclear Periphery | 0.1413 | 0.1598 | 0.2104 | 0.0008 | 0.0024 | 0.3001 | 0.0063 | 0.0109 | 0.0176 | 0 | 0.0639 | 0.065 | – | – | – | – | – | – |
| Nucleolus | 0.0843 | 0.0113 | 0.0236 | 0 | 0.053 | 0.0023 | 0.0043 | 0.0172 | 0.002 | 0 | 0.0088 | 0.0263 | – | – | – | – | – | – |
| Nucleus | 0.0498 | 0.0221 | 0.0576 | 0.0016 | 0.0085 | 0.1081 | 0.161 | 0.0141 | 0.0158 | 0 | 0.0046 | 0.0554 | – | – | – | – | – | – |
| Peroxisomes | 0.0051 | 0.0009 | 0.0023 | 0 | 0.0027 | 0.0001 | 0.01 | 0.0027 | 0.0027 | 0 | 0.002 | 0.0529 | – | – | – | – | – | – |
| Punctate Nuclear | 0.0101 | 0.0019 | 0.0114 | 0.0001 | 0.0596 | 0.0057 | 0.0156 | 0.0091 | 0.0058 | 0 | 0.0038 | 0.0028 | – | – | – | – | – | – |
| Vacuole | 0.0291 | 0.0174 | 0.0259 | 0 | 0.0015 | 0.0071 | 0.0763 | 0.0692 | 0.0266 | 0 | 0.0163 | 0.0604 | – | – | – | – | – | – |
| Vacuole Periphery | 0.0265 | 0.0038 | 0.0196 | 0 | 0.003 | 0.0054 | 0.003 | 0.0021 | 0.0023 | 0 | 0.054 | 0.0083 | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 7257.2173 | 10835.4159 | 12752.3968 | 8418.5033 | 10199.339 | 8158.478 | 10213.2669 | 9423.7701 | 9752.606 | 7369.7299 |
| Translational Efficiency | 1.2877 | 1.2263 | 0.9 | 0.9098 | 0.9932 | 1.1533 | 1.6262 | 1.6042 | 1.1729 | 1.4692 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication |
| Localization | |
| Cell Percentages | ER (87%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | ER-4 |
Ccw12
Ccw12
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Ccw12-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
