ORF
Human Ortholog
Description Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.96 – 0.93 0.9 0.87 0.74 0.78 0.59 0.58 0.96 0.99 1.0 0.88 0.85 0.86 0.95 0.91 0.93 0.87 0.82 0.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0.13 0.13 0.47 0.34 0.54 0.51 0 0 0 0 0 0 0 0 0 0 0 0.06
Nucleus 0 0 0 0 – 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.09
Vac/Vac Membrane 0 0 0 0 – 0 0 0 0 0 0.08 0.1 0 0 0 0 0.05 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 0 0 3 0
Bud 0 0 0 0 – 0 1 1 2 2 5 1 0 0 0 1 1 0 0 3 0 1 2 4
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 3 6 1 0 – 1 0 1 0 1 2 0 1 1 0 2 4 1 0 1 0 0 0 1
Cytoplasm 219 488 72 44 – 124 75 84 128 84 101 81 187 221 253 322 269 258 225 466 99 137 194 146
Endoplasmic Reticulum 4 2 1 0 – 2 0 0 0 0 0 0 4 1 0 23 15 14 0 1 0 0 0 0
Endosome 2 0 0 0 – 0 0 0 0 1 0 1 1 0 1 25 21 22 2 3 0 1 6 8
Golgi 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 1
Mitochondria 1 7 0 2 – 4 11 13 82 37 91 71 0 0 0 16 11 5 1 9 0 4 10 12
Nucleus 1 0 0 2 – 3 2 6 6 5 8 4 2 1 1 1 1 0 0 0 0 2 0 1
Nuclear Periphery 0 1 0 0 – 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 2 1 2 0 0 0 0 1 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 4 4 2 0 0 0 0 0 1
SpindlePole 1 0 0 0 – 1 0 0 1 0 1 0 1 0 0 15 18 10 2 8 0 0 8 18
Vac/Vac Membrane 2 2 0 0 – 2 0 2 6 1 14 14 2 0 1 9 17 25 0 2 1 1 3 3
Unique Cell Count 229 498 74 46 134 83 97 174 108 170 140 194 223 254 368 315 300 236 514 106 158 238 209
Labelled Cell Count 234 507 74 48 137 89 108 225 132 223 174 199 225 256 422 362 340 236 514 106 158 238 209


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.8 5.8 5.3 – 5.5 4.8 5.2 4.4 4.3 4.6 4.3 6.7 5.9 5.1 5.3 6.4 6.3 5.8 5.9 5.7
Std Deviation (1e-4) 0.6 0.5 0.7 0.8 – 1.4 1.4 1.6 1.4 1.2 1.1 0.9 1.1 1.0 1.5 1.6 2.0 1.5 1.4 1.4 1.3
Intensity Change (Log2) – – – -0.14 – -0.08 -0.27 -0.18 -0.4 -0.45 -0.36 -0.43 0.21 0.01 -0.18 -0.14 0.15 0.1 0.01 0.02 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0616 0.6045 0.4273 -0.0781 0.231 0.5654 5.5115 5.7542 5.6996 5.7168 5.1358 5.9098 2.7957 3.1803 3.27 2.9908 3.0202 3.5459
Actin 0.0108 0.0016 0.0006 0.0013 0.0024 0.0044 0.0297 0.0043 0.0168 0.0055 0.0226 0.0098 0.014 0.001 0.0098 0.0015 0.0071 0.0013
Bud 0.0036 0.0028 0.0015 0 0.0014 0.0007 0.0032 0.0078 0.0021 0.009 0.0025 0.0004 0.0035 0.004 0.0035 0.0001 0.0053 0.0006
Bud Neck 0.0019 0.0007 0.0003 0.0001 0.0008 0.0021 0.0027 0.0005 0.003 0.0004 0.0008 0.0012 0.0025 0.0004 0.001 0.0003 0.0006 0.0008
Bud Periphery 0.0042 0.0019 0.0008 0 0.0072 0.0008 0.0082 0.0054 0.0031 0.0057 0.0072 0.0006 0.0071 0.0023 0.0027 0.0001 0.0111 0.0008
Bud Site 0.006 0.0219 0.0022 0 0.0143 0.0003 0.0059 0.0241 0.0058 0.0119 0.0031 0.0007 0.0087 0.0088 0.0088 0.0001 0.001 0.0002
Cell Periphery 0.0005 0.0002 0.0001 0 0.0082 0.0001 0.0005 0.0006 0.0003 0.0005 0.0002 0.0001 0.0008 0.0002 0.0003 0 0.0003 0.0001
Cytoplasm 0.3936 0.5413 0.5684 0.4101 0.6234 0.6321 0.3944 0.5937 0.5778 0.5708 0.5767 0.62 0.358 0.5541 0.5139 0.6451 0.442 0.5411
Cytoplasmic Foci 0.0164 0.0062 0.0105 0.004 0.0164 0.0158 0.0381 0.0082 0.0135 0.0124 0.011 0.0074 0.0211 0.0065 0.0124 0.0093 0.0349 0.0124
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0.0001 0.0002 0.0006 0.0003 0 0.0003 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0062 0.0052 0.0038 0.0023 0.0039 0.0023 0.0071 0.0041 0.0042 0.0035 0.0021 0.0038 0.0037 0.0021 0.0024 0.0028 0.0038 0.0014
Endosome 0.0047 0.0019 0.0033 0.0015 0.0059 0.0045 0.032 0.004 0.005 0.0084 0.0086 0.0038 0.0177 0.0014 0.0071 0.0038 0.0154 0.0024
Golgi 0.0011 0.0002 0.0002 0.0001 0.0031 0.0007 0.0133 0.0007 0.0033 0.0025 0.0021 0.0014 0.0069 0.0002 0.0026 0.0002 0.0065 0.0005
Lipid Particles 0.0015 0.0002 0.0004 0.0001 0.0007 0.0016 0.0179 0.0003 0.0018 0.0034 0.0033 0.0006 0.0149 0.0001 0.0021 0.0001 0.0103 0.0003
Mitochondria 0.0009 0.0003 0.0004 0.0004 0.0026 0.0006 0.0117 0.0013 0.0023 0.0205 0.0017 0.0009 0.0086 0.0004 0.0013 0.0004 0.0261 0.0004
None 0.5281 0.3982 0.3948 0.5622 0.2964 0.3134 0.3769 0.3316 0.342 0.3146 0.3453 0.3379 0.4975 0.4048 0.4199 0.3267 0.3961 0.4262
Nuclear Periphery 0.004 0.0027 0.0017 0.0034 0.0014 0.0018 0.0155 0.0021 0.003 0.0023 0.0018 0.0049 0.0067 0.0022 0.0011 0.002 0.0015 0.0008
Nucleolus 0.001 0.0004 0.0003 0.0004 0.0003 0.0005 0.001 0.0002 0.0005 0.0004 0.0008 0.0001 0.0013 0.0003 0.0002 0.0001 0.0002 0.0001
Nucleus 0.0072 0.0068 0.0058 0.005 0.0035 0.0095 0.0076 0.005 0.0044 0.0027 0.0021 0.0033 0.0035 0.0069 0.0033 0.0032 0.0022 0.0038
Peroxisomes 0.0008 0.0003 0.0004 0.0003 0.0036 0.0016 0.0184 0.0017 0.0061 0.0124 0.0014 0.0009 0.0154 0.0006 0.0042 0.0003 0.03 0.0039
Punctate Nuclear 0.0045 0.0057 0.003 0.0076 0.0015 0.0056 0.0114 0.0025 0.0027 0.0086 0.0037 0.001 0.0041 0.0017 0.002 0.0032 0.0032 0.0023
Vacuole 0.0025 0.0012 0.0012 0.0007 0.0026 0.0015 0.0029 0.0015 0.0014 0.0025 0.0021 0.0009 0.0028 0.0016 0.0011 0.0006 0.0015 0.0005
Vacuole Periphery 0.0004 0.0002 0.0002 0.0001 0.0003 0.0003 0.0013 0.0003 0.0005 0.0013 0.0005 0.0004 0.0008 0.0002 0.0002 0.0001 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.218 18.2662 24.2042 19.8 22.7081 43.7617 37.5975 29.3825 36.7169 48.3422
Translational Efficiency 1.3221 1.7538 0.9208 1.1382 1.1514 1.2404 1.0956 1.1901 0.9253 1.0654

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1568 765 2075 1821 1911 138 662 1397 3479 903 2737 3218

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.74 776.07 971.61 858.97 803.81 909.79 958.60 886.88 746.54 796.51 968.46 871.09
Standard Deviation 78.43 99.96 121.22 127.94 102.11 106.94 119.84 121.17 111.79 111.93 121.02 125.81
Intensity Change Log 2 — 0.197585 0.521776 0.344006 — 0.178679 0.254074 0.141884 — 0.187352 0.382625 0.237796

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000179 0.000444 0.000364 0.000329 0.000079 0.000220 0.000530 0.001707 0.000124 0.000410 0.000404 0.000927
Bud Neck 0.019362 0.014787 0.003797 0.011311 0.002677 0.012385 0.003389 0.007005 0.010197 0.014420 0.003698 0.009442
Bud Site 0.007078 0.016340 0.006848 0.030908 0.002507 0.004103 0.006087 0.032246 0.004568 0.014469 0.006664 0.031489
Cell Periphery 0.000184 0.000162 0.000123 0.000481 0.000144 0.000264 0.000247 0.000178 0.000162 0.000178 0.000153 0.000350
Cytoplasm 0.476796* 0.520463* 0.552344* 0.399943* 0.698967* 0.359800* 0.390357* 0.536762* 0.598833* 0.495910* 0.513164* 0.459339*
Cytoplasmic Foci 0.281377* 0.211151* 0.009055 0.036102 0.099596 0.315147* 0.024475 0.038990 0.181525* 0.227044* 0.012785 0.037356
Eisosomes 0.000261 0.000098 0.000025 0.000123 0.000051 0.000275 0.000080 0.000041 0.000146 0.000125 0.000038 0.000087
Endoplasmic Reticulum 0.000937 0.001498 0.003352 0.001283 0.001064 0.000662 0.005597 0.001810 0.001006 0.001370 0.003895 0.001512
Endosome 0.008544 0.020621 0.001368 0.009125 0.003309 0.030989 0.007949 0.005230 0.005668 0.022205 0.002960 0.007434
Golgi 0.004232 0.004915 0.000084 0.003083 0.000569 0.007922 0.000715 0.000861 0.002220 0.005374 0.000237 0.002118
Lipid Particles 0.008563 0.005128 0.000864 0.002284 0.002010 0.010729 0.004001 0.001507 0.004963 0.005984 0.001623 0.001947
Mitochondria 0.005408 0.004701 0.000429 0.004006 0.000291 0.015829 0.000522 0.000744 0.002597 0.006402 0.000452 0.002590
Mitotic Spindle 0.000525 0.003293 0.005145 0.149845* 0.000236 0.001260 0.028455 0.132544* 0.000366 0.002983 0.010783 0.142335*
None 0.003741 0.003907 0.005158 0.012010 0.006237 0.002005 0.013030 0.006185 0.005112 0.003617 0.007062 0.009481
Nuclear Periphery 0.000262 0.000310 0.001627 0.000949 0.000213 0.000478 0.002218 0.001007 0.000235 0.000336 0.001770 0.000974
Nuclear Periphery Foci 0.000391 0.001555 0.002061 0.002261 0.001089 0.000762 0.010010 0.004795 0.000774 0.001434 0.003984 0.003361
Nucleolus 0.000757 0.001487 0.000164 0.003976 0.000357 0.003630 0.001978 0.001090 0.000537 0.001815 0.000603 0.002723
Nucleus 0.137096* 0.087078 0.344338* 0.215584* 0.152029* 0.128208 0.365445* 0.119351 0.145299* 0.093364 0.349443* 0.173807*
Peroxisomes 0.005516 0.008055 0.001093 0.006216 0.000397 0.009588 0.001346 0.004846 0.002704 0.008289 0.001154 0.005621
Vacuole 0.037353 0.092108 0.061679 0.108651 0.027936 0.093399 0.133269 0.101410 0.032180 0.092305 0.078994 0.105507
Vacuole Periphery 0.001439 0.001899 0.000083 0.001531 0.000242 0.002346 0.000300 0.001691 0.000781 0.001967 0.000135 0.001600

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.04 -2.65 -6.66 0.12 -0.63 -1.71 -8.59 -3.86 -3.13 -1.03 -6.52 -5.74 -5.25 -2.44 -1.74
Bud Neck 2.26 10.04 2.88 0.11 -10.57 -3.80 -4.05 -8.69 0.59 -6.11 -2.84 7.07 -2.94 1.03 -12.11
Bud Site -4.94 0.46 -9.88 -4.82 -10.17 -1.86 -6.19 -12.99 -11.79 -10.28 -6.76 -2.64 -16.45 -7.74 -14.18
Cell Periphery 1.41 3.95 -1.27 -1.96 -2.86 -3.81 -5.24 0.74 3.97 5.37 -1.05 0.63 -1.36 -0.59 -1.63
Cytoplasm -3.12 0.60 18.73 17.08 19.04 15.72 38.99 33.24 -1.02 -6.92 10.28 25.47 38.40 14.09 12.54
Cytoplasmic Foci 7.75 48.06 41.06 23.40 -12.54 -12.45 24.49 10.29 14.95 -11.09 -6.02 49.58 35.93 26.15 -17.73
Eisosomes 8.43 12.71 9.38 1.36 -8.48 -6.63 -9.60 -0.55 6.58 9.21 1.68 11.43 8.29 5.96 -5.80
Endoplasmic Reticulum -2.65 -11.19 -5.89 -1.57 8.55 2.53 -17.28 -5.54 -8.78 15.03 -2.14 -16.95 -7.97 -3.03 13.59
Endosome -6.44 14.31 -1.88 5.36 -11.85 -6.70 -5.47 -7.79 5.37 -1.63 -9.85 8.70 -6.95 7.34 -12.45
Golgi -0.44 11.91 4.66 2.78 -3.61 -1.89 0.73 -2.20 1.81 -2.06 -2.81 12.12 3.10 3.64 -4.45
Lipid Particles 3.10 11.90 9.42 2.72 -9.28 -5.59 -5.39 -1.81 5.37 4.33 -1.23 10.39 7.89 4.27 -5.73
Mitochondria 0.65 7.91 3.85 1.79 -3.26 -1.88 -2.23 -3.96 1.84 0.80 -2.25 7.04 2.56 2.91 -3.12
Mitotic Spindle -2.70 -5.32 -27.04* -26.57* -25.96* -1.38 -5.30 -22.14 -21.96 -16.98 -3.09 -7.53 -34.93 -34.24* -32.12*
None -0.01 -0.98 -4.33 -3.30 -3.95 3.76 -1.45 -0.12 -4.21 1.45 1.61 -0.42 -3.42 -3.76 -2.97
Nuclear Periphery -1.42 -27.46 -16.40 -16.11 -3.56 -3.10 -24.94 -19.23 -10.88 9.89 -3.53 -37.79 -22.39 -20.68 0.90
Nuclear Periphery Foci -4.61 -6.86 -8.36 -7.14 -5.10 2.01 -6.01 -9.56 -9.75 -0.05 -3.90 -8.34 -12.62 -11.66 -5.02
Nucleolus -1.51 2.83 -9.03 -8.02 -9.36 -2.02 -4.49 -6.19 -1.01 -3.32 -2.73 -1.88 -10.86 -8.13 -10.40
Nucleus 8.52 -35.45 -3.95 -11.12 28.29 1.95 -20.79 7.00 1.01 23.56 10.61 -41.84 1.00 -8.82 39.31
Peroxisomes -2.26 7.41 0.04 2.01 -5.62 -4.74 -2.34 -7.84 2.40 -6.33 -6.69 5.11 -4.51 3.16 -7.72
Vacuole -11.51 -19.08 -23.36 -9.65 -8.34 -6.33 -19.69 -21.81 -5.12 5.25 -13.98 -27.66 -32.23 -11.03 -4.66
Vacuole Periphery -1.10 4.66 2.17 3.89 -3.56 -1.99 -0.89 -1.55 1.54 -1.37 -3.97 4.88 0.49 4.17 -3.61
ORF
Human Ortholog
Description Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (68%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR126C

YLR126C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr126c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available