Standard name
Human Ortholog
Description Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.09 0 0.06 0.06 0.05 0.06 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.13 0 0 0.05 0 0 0 0 0 0 0 0.1 0.08 0.13 0.48 0.59 0.44 0.07 0.09 0.09 0.13 0.14 0.13
Mitochondria 0.94 0.89 0.96 0.94 0.92 0.9 0.91 0.88 0.93 0.92 0.92 0.91 0.85 0.89 0.8 0.1 0.16 0.05 0.82 0.73 0.74 0.7 0.7 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.06 0.07 0.06 0.09 0.2 0.2 0.15 0.18 0.16 0.18 0.21 0 0 0 0.12 0.09 0.16 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0.07 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.1 0 0 0.05 0 0 0.05 0 0 0 0 0.05 0.06 0.06 0.15 0.17 0.17 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 6 4 2 0 0 0 1 1 0 0 0 9 8 9 17 12 13 4 13 9 11 20 41
Bud 1 2 0 2 0 0 0 2 0 2 0 3 0 3 2 7 2 1 1 1 0 2 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 – – – – – –
Cell Periphery 1 5 6 0 0 0 0 0 0 0 1 0 5 8 4 24 8 13 0 0 0 0 0 0
Cytoplasm 4 1 4 3 3 5 10 10 7 6 7 5 3 4 13 19 11 7 1 0 1 0 0 0
Endoplasmic Reticulum 0 1 0 0 1 0 1 4 2 1 1 0 14 6 15 8 8 5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 6 2 0 2 3 1 1 3 5
Golgi 0 25 5 2 7 0 2 6 3 4 2 3 29 30 45 152 154 65 13 20 13 32 42 43
Mitochondria 170 175 144 101 139 155 208 200 150 158 146 159 243 328 281 30 42 8 151 154 114 174 220 211
Nucleus 1 0 0 0 1 3 0 2 2 1 1 1 0 1 0 1 0 5 0 0 0 1 0 1
Nuclear Periphery 4 5 0 0 1 0 3 4 3 1 6 6 1 3 3 8 2 2 1 0 0 2 4 2
Nucleolus 26 12 11 6 14 35 45 35 29 27 28 37 9 5 7 37 24 24 3 0 1 6 5 4
Peroxisomes 0 1 5 0 0 2 1 0 0 0 0 0 10 12 9 15 8 9 2 2 11 8 9 16
SpindlePole 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 5 5 6 1 0 0 0 0 0
Vac/Vac Membrane 12 19 4 2 8 5 5 11 4 5 4 3 15 23 22 48 45 25 0 12 0 5 6 9
Unique Cell Count 180 196 150 108 151 172 228 226 162 171 159 174 285 367 352 315 262 147 185 212 155 249 317 350
Labelled Cell Count 220 252 183 118 174 205 277 276 201 205 197 217 338 433 413 377 326 183 185 212 155 249 317 350


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.5 25.4 29.5 32.3 43.0 37.6 32.1 30.2 31.1 26.1 29.2 25.7 21.8 19.9 19.9 29.3 31.8 31.7 24.4 24.7 24.1
Std Deviation (1e-4) 12.6 9.2 10.9 11.7 15.2 14.8 13.7 12.4 13.2 11.3 12.2 11.1 7.6 7.2 7.8 14.9 15.9 16.3 11.4 11.1 11.1
Intensity Change (Log2) – – – 0.13 0.54 0.35 0.12 0.04 0.08 -0.17 -0.01 -0.2 -0.44 -0.57 -0.57 -0.01 0.11 0.1 -0.28 -0.26 -0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1.3 1.0 2.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.5 -0.5 1.7
Cytoplasm 0 0 0 0.9 0.9 0.8 0 0.8 0 0 0 0.6 1.6 0.8 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.6 0 0 0 0 0 0 0 2.5 2.0 3.2 9.6 11.1 8.3
Mitochondria -0.9 -1.4 -2.0 -1.8 -2.6 -1.3 -1.4 -1.5 -1.7 -3.4 -2.4 -4.6 -18.0 -15.7 -15.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.6 0.6 3.3 3.3 2.4 2.8 2.3 2.7 3.5 -2.0 0 -2.9 1.5 0.6 2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0.1 0 0 0.7 0 1.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.2 0 0 1.1 0 0 0 0 1.3 1.7 1.7 4.0 4.4 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1223 9.6737 9.3568 9.0282 8.4832 10.4488 9.9555 10.9197 10.5543 11.0585 12.5286 10.7397 12.0072 12.9508 12.3876 13.7755 13.5293 12.3598
Actin 0.043 0.0105 0.0008 0.0001 0.0426 0.0023 0.0194 0.013 0.0191 0.0222 0.0097 0.0084 0.0593 0.0216 0.0099 0.0004 0.0111 0.0118
Bud 0.0003 0.0003 0 0 0.0005 0.0001 0.0007 0.0002 0.0006 0.0005 0.0006 0.0002 0.0005 0.0007 0.0004 0.0001 0.0003 0
Bud Neck 0.0007 0.0001 0 0 0.004 0.0001 0.0016 0.0001 0.0007 0.0001 0.0001 0 0.0004 0.0004 0.0002 0 0.0002 0
Bud Periphery 0.0007 0.0006 0.0001 0 0.0009 0.0002 0.0017 0.0003 0.0008 0.0017 0.0021 0.0005 0.0008 0.0015 0.0008 0.0001 0.0004 0.0001
Bud Site 0.0009 0.0012 0.0001 0 0.0012 0.0002 0.0038 0.0006 0.0019 0.0008 0.0004 0.0002 0.0013 0.0021 0.0021 0 0.0013 0.0001
Cell Periphery 0.0002 0.0003 0 0 0.0003 0.0002 0.0005 0.0001 0.0004 0.0003 0.0001 0.0001 0.0002 0.0003 0.0006 0 0.0001 0.0001
Cytoplasm 0.0135 0.0001 0.0001 0 0.0006 0 0.0028 0.0001 0.0011 0.0002 0.0001 0 0.0027 0.0001 0.0002 0 0.0001 0
Cytoplasmic Foci 0.0129 0.0044 0.0013 0 0.0048 0.002 0.0055 0.0074 0.0126 0.0009 0.0265 0.0049 0.0177 0.0054 0.0036 0.0001 0.0282 0.0005
Eisosomes 0.0006 0.0002 0.0003 0 0.0003 0.0002 0.0006 0.0003 0.0003 0.0028 0.0002 0.0002 0.0004 0.0006 0.0017 0 0.0002 0.0002
Endoplasmic Reticulum 0.0089 0.0008 0 0 0.0005 0 0.0023 0 0.001 0.0016 0.0005 0.0001 0.0009 0.0004 0.0001 0 0.0001 0.0001
Endosome 0.0084 0.0107 0.0033 0.0004 0.0145 0.002 0.0102 0.0128 0.0178 0.0061 0.0122 0.0024 0.0491 0.0184 0.0048 0.0002 0.0039 0.0052
Golgi 0.0056 0.0149 0.0224 0.0004 0.0191 0.0071 0.0181 0.0227 0.0154 0.006 0.0166 0.0057 0.0247 0.0286 0.0174 0.0003 0.0092 0.0147
Lipid Particles 0.02 0.0086 0.0007 0.0005 0.009 0.0145 0.0147 0.004 0.0119 0.0032 0.0223 0.0055 0.0264 0.0135 0.015 0.0001 0.0157 0.012
Mitochondria 0.8446 0.9143 0.9494 0.9958 0.8941 0.9578 0.8857 0.9232 0.8918 0.946 0.8872 0.9637 0.7747 0.868 0.9068 0.9979 0.8836 0.9453
None 0.0035 0 0.0133 0 0.0002 0.0001 0.0032 0.0001 0.0003 0.0007 0.0002 0.0001 0.0011 0.0001 0.0001 0 0.0001 0
Nuclear Periphery 0.0109 0.0101 0 0 0.0006 0.0001 0.0018 0 0.0014 0.0018 0.0008 0.002 0.0036 0.0012 0 0 0.0001 0.0002
Nucleolus 0.0011 0.0009 0 0 0.0005 0.0003 0.0014 0.0003 0.0014 0.0001 0.0003 0.0001 0.0073 0.0004 0.0006 0 0.0001 0.0001
Nucleus 0.0018 0.0003 0 0 0.0004 0.0001 0.0017 0 0.0003 0.0002 0.0001 0.0001 0.0048 0.0002 0.0001 0 0.0001 0.0001
Peroxisomes 0.0142 0.0096 0.0065 0.0003 0.0018 0.0093 0.0147 0.0113 0.0122 0.003 0.0162 0.0038 0.0069 0.0304 0.0317 0.0004 0.0429 0.0021
Punctate Nuclear 0.0045 0 0 0 0.0003 0 0.0019 0 0.0013 0.0002 0.0006 0.0001 0.0046 0 0 0 0.0015 0
Vacuole 0.0011 0.0033 0.0002 0.0003 0.0019 0.0007 0.0036 0.0008 0.0031 0.0006 0.0009 0.0003 0.0071 0.0025 0.0013 0.0001 0.0003 0.0009
Vacuole Periphery 0.0027 0.0084 0.0016 0.002 0.0018 0.0027 0.0042 0.0028 0.0046 0.0012 0.0021 0.0018 0.0055 0.0035 0.0024 0.0002 0.0006 0.0063

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3975 3.4465 2.5574 5.1609 1.4996 5.3945 6.7952 4.7039 6.1385 5.4112
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Put1

Put1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Put1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available