Standard name | |
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Human Ortholog | |
Description | Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 2 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 13 | 0 | 3 | 1 | 4 | – | 1 | 1 | 6 | 4 | 1 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 214 | 331 | 197 | 146 | 226 | 190 | 196 | – | 186 | 198 | 202 | 209 | 302 | 308 | 285 | – | – | – | 207 | 317 | 192 | 184 | 249 | 315 |
Endoplasmic Reticulum | 2 | 3 | 2 | 1 | 0 | 2 | 1 | – | 1 | 2 | 3 | 2 | 1 | 2 | 1 | – | – | – | 1 | 1 | 1 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 2 | 2 | 0 | 1 | – | 1 | 1 | 0 | 3 | 1 | 0 | 3 | – | – | – | 2 | 3 | 0 | 0 | 4 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 1 | 3 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 1 | 3 | 11 | 20 | – | 61 | 34 | 102 | 74 | 0 | 1 | 0 | – | – | – | 0 | 2 | 1 | 1 | 3 | 4 |
Nucleus | 0 | 2 | 2 | 3 | 1 | 0 | 3 | – | 1 | 4 | 4 | 8 | 1 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 2 | 1 |
Vac/Vac Membrane | 0 | 2 | 4 | 4 | 8 | 3 | 6 | – | 11 | 7 | 8 | 20 | 2 | 2 | 6 | – | – | – | 0 | 1 | 0 | 2 | 1 | 1 |
Unique Cell Count | 215 | 336 | 204 | 151 | 234 | 201 | 214 | 235 | 238 | 276 | 282 | 306 | 313 | 292 | 213 | 335 | 203 | 198 | 273 | 348 | ||||
Labelled Cell Count | 217 | 341 | 219 | 157 | 243 | 207 | 232 | 264 | 249 | 328 | 322 | 309 | 316 | 295 | 213 | 335 | 203 | 198 | 273 | 348 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 7.8 | 7.5 | 7.3 | 7.7 | 6.3 | 6.3 | – | 5.6 | 5.5 | 5.4 | 5.4 | 8.0 | 8.1 | 8.0 | – | – | – | 7.5 | 7.7 | 7.7 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.3 | 1.2 | 2.2 | 2.0 | 1.6 | – | 1.7 | 1.3 | 2.0 | 2.2 | 1.1 | 1.3 | 1.8 | – | – | – | 1.2 | 2.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.03 | -0.24 | -0.24 | – | -0.43 | -0.44 | -0.47 | -0.48 | 0.09 | 0.1 | 0.08 | – | – | – | -0.01 | 0.04 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -3.2 | -2.8 | -3.2 | -2.3 | – | -3.5 | -3.6 | -2.3 | -2.9 | -4.1 | -4.1 | -4.4 | – | – | – |
Cytoplasm | 0.1 | 0 | -1.0 | -2.1 | – | -5.5 | -4.5 | -6.8 | -6.6 | 1.6 | 1.4 | 0.7 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 3.4 | 4.5 | – | 7.8 | 5.6 | 9.8 | 7.9 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0.9 | 0 | 0 | – | 1.6 | 0.7 | 0.7 | 2.6 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4853 | 2.6649 | 2.1004 | 1.3699 | 1.5773 | 2.327 | 2.5182 | 3.5932 | 3.1178 | 2.6158 | 2.8688 | 3.1902 | 3.6203 | 4.5213 | 3.9783 | 3.7406 | 3.3493 | 3.7394 |
Actin | 0.0322 | 0.0008 | 0.0229 | 0.0317 | 0.0034 | 0.0135 | 0.0253 | 0.0042 | 0.0018 | 0.0007 | 0.0012 | 0.0009 | 0.0504 | 0.0007 | 0.0138 | 0.0507 | 0.0186 | 0.0077 |
Bud | 0.0023 | 0.0065 | 0.0033 | 0.0157 | 0.0019 | 0.0003 | 0.0028 | 0.0051 | 0.0109 | 0.0002 | 0.001 | 0.0118 | 0.001 | 0.0001 | 0.0018 | 0.0225 | 0.0007 | 0.0007 |
Bud Neck | 0.0009 | 0.0004 | 0.0006 | 0.0025 | 0.0009 | 0.0009 | 0.0056 | 0.0003 | 0.0008 | 0.0004 | 0.0004 | 0.0018 | 0.0019 | 0.0001 | 0.009 | 0.0014 | 0.001 | 0.004 |
Bud Periphery | 0.0015 | 0.0034 | 0.0024 | 0.0093 | 0.0008 | 0.0002 | 0.003 | 0.0018 | 0.0048 | 0.0001 | 0.0011 | 0.0057 | 0.0012 | 0 | 0.0026 | 0.0121 | 0.0006 | 0.0007 |
Bud Site | 0.007 | 0.0071 | 0.0057 | 0.0116 | 0.0006 | 0.0002 | 0.0113 | 0.0096 | 0.0142 | 0.0003 | 0.0035 | 0.0009 | 0.0029 | 0.0008 | 0.004 | 0.0047 | 0.0022 | 0.0014 |
Cell Periphery | 0.0007 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.7025 | 0.8843 | 0.7991 | 0.498 | 0.7531 | 0.8881 | 0.6832 | 0.8504 | 0.8556 | 0.7579 | 0.6657 | 0.8739 | 0.7708 | 0.9421 | 0.8693 | 0.7985 | 0.7957 | 0.9121 |
Cytoplasmic Foci | 0.0266 | 0.0103 | 0.0141 | 0.0451 | 0.0161 | 0.0099 | 0.025 | 0.0129 | 0.0048 | 0.0071 | 0.0203 | 0.0068 | 0.0261 | 0.0089 | 0.0147 | 0.0177 | 0.0569 | 0.0105 |
Eisosomes | 0.0004 | 0 | 0.0008 | 0.0003 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0082 | 0.0041 | 0.0029 | 0.0022 | 0.0031 | 0.0024 | 0.0079 | 0.0022 | 0.0022 | 0.0029 | 0.0038 | 0.0019 | 0.007 | 0.0012 | 0.0043 | 0.003 | 0.0033 | 0.0029 |
Endosome | 0.0407 | 0.0112 | 0.03 | 0.0931 | 0.067 | 0.0155 | 0.0306 | 0.0054 | 0.0047 | 0.0047 | 0.1239 | 0.002 | 0.0228 | 0.0028 | 0.0146 | 0.007 | 0.0389 | 0.008 |
Golgi | 0.0096 | 0.0009 | 0.0127 | 0.0964 | 0.0134 | 0.0039 | 0.0075 | 0.0012 | 0.0041 | 0.0013 | 0.0123 | 0.0001 | 0.0084 | 0.0001 | 0.004 | 0.0032 | 0.0194 | 0.0047 |
Lipid Particles | 0.0089 | 0.0003 | 0.0059 | 0.0452 | 0.0035 | 0.0131 | 0.0073 | 0.0021 | 0.0003 | 0.0018 | 0.0034 | 0 | 0.0061 | 0.0001 | 0.0032 | 0.0011 | 0.0096 | 0.0045 |
Mitochondria | 0.0082 | 0.0008 | 0.0111 | 0.0456 | 0.0403 | 0.0011 | 0.0162 | 0.0013 | 0.0021 | 0.0396 | 0.0579 | 0.0002 | 0.0066 | 0.0001 | 0.0022 | 0.0019 | 0.0048 | 0.0034 |
None | 0.1144 | 0.0628 | 0.0729 | 0.0305 | 0.0315 | 0.0361 | 0.1254 | 0.0932 | 0.0842 | 0.1686 | 0.0725 | 0.0814 | 0.068 | 0.0386 | 0.0361 | 0.0448 | 0.0212 | 0.0219 |
Nuclear Periphery | 0.0079 | 0.0014 | 0.0019 | 0.0016 | 0.0148 | 0.0041 | 0.0111 | 0.001 | 0.0016 | 0.0008 | 0.0016 | 0.001 | 0.0073 | 0.0006 | 0.0028 | 0.0096 | 0.0023 | 0.002 |
Nucleolus | 0.003 | 0.0001 | 0.0006 | 0.001 | 0.0039 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 |
Nucleus | 0.0063 | 0.0024 | 0.0034 | 0.0043 | 0.0181 | 0.0038 | 0.0089 | 0.0037 | 0.0043 | 0.0017 | 0.0015 | 0.0079 | 0.0039 | 0.0018 | 0.0036 | 0.0089 | 0.0051 | 0.0069 |
Peroxisomes | 0.0052 | 0.0005 | 0.0029 | 0.0484 | 0.0013 | 0.001 | 0.0134 | 0.0016 | 0.0004 | 0.0102 | 0.0052 | 0.0001 | 0.0085 | 0.0001 | 0.0095 | 0.0029 | 0.0042 | 0.005 |
Punctate Nuclear | 0.0085 | 0.0007 | 0.0028 | 0.0022 | 0.0143 | 0.0033 | 0.0057 | 0.0025 | 0.0014 | 0.0004 | 0.0005 | 0.0021 | 0.0025 | 0.0009 | 0.0021 | 0.007 | 0.0122 | 0.0023 |
Vacuole | 0.0037 | 0.0017 | 0.0026 | 0.0118 | 0.0061 | 0.0017 | 0.0063 | 0.0011 | 0.001 | 0.0008 | 0.0204 | 0.0009 | 0.0023 | 0.0007 | 0.0017 | 0.0011 | 0.0022 | 0.0008 |
Vacuole Periphery | 0.0013 | 0.0002 | 0.0011 | 0.0028 | 0.0056 | 0.0007 | 0.0014 | 0.0001 | 0.0003 | 0.0002 | 0.0034 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0004 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.6719 | 11.9822 | 11.3809 | 16.3533 | 12.5126 | 11.7889 | 13.3267 | 15.8633 | 15.1098 | 15.3925 |
Translational Efficiency | 1.0453 | 1.0634 | 1.1674 | 0.706 | 0.8687 | 1.0819 | 1.1121 | 0.7561 | 0.733 | 0.9362 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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