Standard name
Human Ortholog
Description Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 – – 0 0.05 0.06 0.13 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 – – 0 0.1 0.09 0.05 0 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.07 0.08 0.08 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0.08 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0 0.08 0.05 0.12 0 0 0
Cytoplasm 0.78 0.84 0.77 0.8 0.82 0.86 0.78 0.81 0.76 0.74 0.61 0.65 0.77 0.85 0.9 – – 0.87 0.23 0.33 0.12 0.29 0.3 0.35
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – 0 0.05 0.06 0.13 0.07 0.05 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.18 0.21 0.32 0.43 0.52 0.42 0 0 0 – – 0 0 0 0.08 0.1 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.06 0 0.13 0.18 0.09 0.11 0 0 0 0 0 0.09 0.05 0 – – 0.09 0.33 0.28 0.24 0.33 0.36 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 4 12 4 0 1 0 4 2 0 3 2 33 26 19 – – 4 18 10 24 16 10 13
Bud 6 5 7 5 3 9 9 18 20 28 30 20 12 14 10 – – 2 35 15 9 12 17 18
Bud Neck 0 0 1 0 0 0 0 4 1 3 3 2 9 4 6 – – 3 24 13 15 10 8 11
Bud Site 1 0 1 0 0 0 1 2 2 4 3 2 1 1 0 – – 0 – – – – – –
Cell Periphery 27 12 17 3 1 2 5 5 2 1 7 6 22 27 43 – – 2 28 8 21 10 12 8
Cytoplasm 249 141 134 152 196 307 301 424 310 312 248 294 527 715 827 – – 180 84 57 22 83 90 141
Endoplasmic Reticulum 13 10 6 2 1 1 2 1 1 1 1 0 37 21 25 – – 8 18 10 23 20 15 30
Endosome 1 0 2 5 2 2 3 7 2 0 1 4 6 5 1 – – 2 4 2 2 5 4 2
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 2 0 0 – – 0 1 0 0 0 1 2
Mitochondria 4 2 3 3 5 48 69 110 132 179 213 190 2 2 0 – – 9 10 5 14 28 22 21
Nucleus 5 1 0 8 9 5 8 3 4 1 4 2 11 15 11 – – 8 0 0 0 0 0 1
Nuclear Periphery 1 0 0 2 0 1 2 4 7 3 1 1 0 0 0 – – 1 1 0 0 0 0 0
Nucleolus 0 1 1 0 0 0 0 1 2 0 0 0 1 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 4 0 0 0 2 4 2 1 1 0 0 3 0 – – 0 0 0 0 0 0 1
SpindlePole 2 0 5 3 0 0 6 5 9 9 14 12 19 16 5 – – 1 7 0 1 1 2 4
Vac/Vac Membrane 27 10 7 24 43 31 43 23 11 13 10 20 63 46 17 – – 19 119 50 44 94 111 134
Unique Cell Count 320 168 175 190 238 355 384 526 407 421 407 449 684 840 916 207 359 177 186 289 306 399
Labelled Cell Count 354 186 200 212 260 407 451 615 507 555 539 555 745 895 964 239 359 177 186 289 306 399


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.1 5.8 5.8 5.0 3.9 3.6 3.6 3.3 3.0 3.2 3.1 6.5 6.0 6.0 4.6 4.9 5.6 5.8 6.1 6.1
Std Deviation (1e-4) 2.5 2.4 3.6 3.2 2.5 2.2 1.7 1.3 1.2 1.1 1.3 1.3 2.7 1.7 1.5 1.0 1.0 1.3 2.0 2.2 1.9
Intensity Change (Log2) – – – -0.01 -0.23 -0.59 -0.7 -0.71 -0.81 -0.95 -0.88 -0.9 0.15 0.04 0.03 -0.34 -0.27 -0.07 -0.02 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -4.1 0 0 0 0 0 0 0 -1.1 -2.4 0 – – -2.4
Bud -0.7 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Cell Periphery -3.4 -4.6 0 0 0 0 0 0 0 -3.7 -3.8 -2.7 – – -3.9
Cytoplasm 0.8 1.4 2.9 0.5 1.1 -0.1 -0.6 -3.6 -2.7 0.1 2.8 5.1 – – 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.1 0 0 – – 0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – 1.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0
Vacuole 3.0 4.3 2.0 2.8 0.2 -0.8 -0.6 -1.0 0.3 2.2 0.8 0 – – 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1055 2.1146 2.0811 1.483 4.1854 2.1564 3.2177 3.8705 3.548 3.0047 2.5398 3.6719 3.5782 5.0001 3.4976 3.7479 3.5477 3.83
Actin 0.0646 0.0023 0.0031 0.002 0.0431 0.0098 0.0242 0.0039 0.0106 0.0001 0.002 0.0079 0.0266 0.0006 0.0064 0.0015 0.0095 0.0041
Bud 0.0033 0.0019 0.0163 0.0196 0.0068 0.006 0.0052 0.0133 0.0049 0.0022 0.009 0.0052 0.0058 0.0019 0.0072 0.005 0.0004 0.0038
Bud Neck 0.0036 0.0022 0.0057 0.0119 0.0062 0.0081 0.0031 0.0041 0.0043 0.0058 0.0056 0.0269 0.0032 0.0076 0.0082 0.0031 0.0026 0.0313
Bud Periphery 0.0145 0.0034 0.0545 0.0488 0.0213 0.0439 0.0173 0.0316 0.0271 0.006 0.034 0.0383 0.0267 0.0047 0.0156 0.0744 0.0008 0.0127
Bud Site 0.0138 0.047 0.0072 0.0102 0.0095 0.0032 0.0149 0.0855 0.0095 0.0049 0.0085 0.0024 0.0077 0.0492 0.0236 0.0015 0.0014 0.0026
Cell Periphery 0.0323 0.0151 0.0167 0.0259 0.0454 0.0305 0.0281 0.0282 0.0366 0.0108 0.0097 0.0213 0.0342 0.0485 0.0337 0.0154 0.0041 0.023
Cytoplasm 0.1651 0.2435 0.232 0.181 0.1341 0.2318 0.1659 0.1684 0.196 0.1111 0.172 0.1783 0.2358 0.2708 0.2193 0.1141 0.2162 0.2216
Cytoplasmic Foci 0.0741 0.0468 0.0262 0.0402 0.0432 0.0284 0.0557 0.0328 0.0573 0.0624 0.047 0.0808 0.0461 0.0711 0.0426 0.1415 0.0333 0.0547
Eisosomes 0.0008 0.0003 0.0004 0.0001 0.0001 0.0002 0.0008 0.0001 0.0017 0 0.001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0133 0.0079 0.0146 0.0053 0.0108 0.016 0.0215 0.0051 0.0167 0.0063 0.0066 0.0086 0.0137 0.0093 0.0107 0.0053 0.0114 0.0102
Endosome 0.08 0.0884 0.1158 0.2345 0.1719 0.1627 0.1338 0.0943 0.1105 0.1624 0.1026 0.1446 0.133 0.1007 0.1303 0.2256 0.146 0.166
Golgi 0.0078 0.0023 0.0108 0.0038 0.0161 0.0082 0.0123 0.0025 0.0086 0.0062 0.0029 0.0067 0.0121 0.0066 0.0076 0.01 0.0199 0.0071
Lipid Particles 0.02 0.0144 0.0052 0.0108 0.0214 0.0076 0.0635 0.0221 0.0331 0.0186 0.0314 0.0482 0.0738 0.0277 0.0361 0.0808 0.0442 0.0412
Mitochondria 0.0039 0.0027 0.0066 0.0029 0.0097 0.0168 0.0204 0.0027 0.0053 0.0011 0.0017 0.0095 0.0053 0.0044 0.0087 0.0015 0.0035 0.0077
None 0.3139 0.3414 0.2612 0.1486 0.0058 0.2209 0.202 0.2054 0.2497 0.1509 0.1981 0.1942 0.1775 0.1221 0.2001 0.1075 0.1824 0.158
Nuclear Periphery 0.0131 0.0028 0.0045 0.0031 0.0037 0.0075 0.0205 0.0014 0.0027 0.0014 0.002 0.0027 0.003 0.0074 0.0061 0.001 0.0025 0.0025
Nucleolus 0.0034 0.0055 0.0012 0.0026 0.0012 0.0018 0.0036 0.0058 0.0025 0.0041 0.0061 0.0016 0.0014 0.0017 0.0025 0.0009 0.0008 0.0012
Nucleus 0.0085 0.0043 0.0078 0.004 0.0036 0.0086 0.0054 0.0088 0.004 0.0049 0.0056 0.0033 0.0023 0.0035 0.0038 0.0018 0.0032 0.0025
Peroxisomes 0.0131 0.0011 0.0011 0.0012 0.0082 0.0012 0.0058 0.0009 0.0028 0.0025 0.0022 0.004 0.0022 0.0039 0.0035 0.0024 0.0058 0.0015
Punctate Nuclear 0.0338 0.0013 0.0006 0.0014 0.001 0.0089 0.0042 0.0026 0.0017 0.0005 0.0008 0.002 0.0011 0.0011 0.0029 0.0019 0.0008 0.0007
Vacuole 0.1118 0.1583 0.1927 0.2216 0.4104 0.1686 0.1732 0.272 0.2066 0.4235 0.3379 0.2019 0.1792 0.2343 0.213 0.1937 0.301 0.2262
Vacuole Periphery 0.0053 0.0069 0.0157 0.0204 0.0269 0.0095 0.0184 0.0083 0.0078 0.0143 0.0133 0.0114 0.0091 0.0226 0.0177 0.011 0.01 0.0211

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.8416 29.0918 42.7674 39.4704 27.4658 38.6622 35.3809 30.9031 39.0081 35.4521
Translational Efficiency 0.2468 0.5128 0.627 0.5728 0.3987 0.2217 0.3942 0.4065 0.372 0.2571

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
Localization
Cell Percentages vacuole (30%), cell periphery (8%), mixed (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Thi7

Thi7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thi7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available