Standard name
Human Ortholog
Description Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0.07 0 0 0 0 0 0.05
Bud 0 0 – 0 0 0 0.05 0.08 0.09 0.05 0.05 0 0 0 0 0.06 – 0.09 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – 0.06 0 0 0 0 0 0
Cytoplasm 0.33 0.43 – 0.55 0.56 0.37 0.27 0.28 0.2 0.16 0.18 0.2 0.77 0.87 0.88 0.41 – 0.25 0.05 0.17 0.09 0.1 0.1 0.11
Endoplasmic Reticulum 0 0.14 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 – 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0.06 0.06 0 0 0 0 0 0 0 0.05 0.05 0.07 – 0.2 0.08 0 0.05 0.06 0.06 0.05
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.68 0.76 – 0.43 0.23 0.51 0.69 0.58 0.82 0.82 0.88 0.79 0 0 0 0.15 – 0.11 0.71 0.61 0.68 0.65 0.61 0.59
Nucleus 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0
SpindlePole 0 0 – 0.06 0.14 0.06 0.1 0.18 0.05 0.1 0 0.09 0 0 0 0.15 – 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0.07 0.06 0 0 0 0 0.05 0 0 0.06 0.11 – 0.16 0.05 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 – 1 2 0 0 1 0 1 0 0 11 4 0 9 – 6 2 2 1 5 18 35
Bud 5 0 – 3 3 1 17 23 19 13 11 11 5 2 0 7 – 8 0 0 0 1 10 14
Bud Neck 0 0 – 1 8 3 5 4 1 0 1 2 9 6 3 2 – 0 1 1 0 3 11 17
Bud Site 0 0 – 0 1 0 4 1 2 5 2 1 0 0 0 0 – 1 – – – – – –
Cell Periphery 0 0 – 0 1 3 1 2 1 1 1 1 3 11 8 8 – 5 2 0 0 1 3 10
Cytoplasm 64 9 – 47 121 79 97 78 40 41 38 57 219 543 667 47 – 22 12 12 3 32 62 76
Endoplasmic Reticulum 6 3 – 0 1 0 1 0 0 0 1 0 18 2 2 5 – 5 5 0 0 8 6 9
Endosome 0 0 – 0 13 12 1 7 1 2 0 1 10 29 39 8 – 18 20 2 2 20 37 36
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 – 0 0 0 0 1 1 3
Mitochondria 133 16 – 37 51 108 246 161 165 206 187 222 9 14 5 17 – 10 175 47 28 220 365 427
Nucleus 0 0 – 0 0 3 8 13 4 6 1 8 0 0 1 0 – 3 0 0 0 0 2 2
Nuclear Periphery 1 0 – 0 0 0 0 0 0 4 1 0 0 0 1 0 – 0 0 0 1 0 3 2
Nucleolus 0 0 – 0 0 0 0 2 1 0 0 1 0 0 0 1 – 0 0 0 0 2 1 2
Peroxisomes 0 0 – 0 3 6 3 1 0 1 0 0 0 12 23 3 – 5 2 0 0 6 5 3
SpindlePole 3 0 – 5 31 13 36 49 11 25 7 26 8 13 15 17 – 11 5 1 1 10 23 31
Vac/Vac Membrane 7 0 – 1 9 15 20 5 3 8 3 15 6 22 42 13 – 14 11 5 0 11 25 33
Unique Cell Count 195 21 86 218 211 356 276 201 250 213 280 283 623 762 114 88 248 77 42 340 602 729
Labelled Cell Count 220 29 95 244 243 439 347 248 313 253 345 298 658 807 138 108 248 77 42 340 602 729


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.3 – 4.5 5.0 4.1 3.9 4.4 3.5 3.7 3.5 3.9 4.8 5.0 4.9 5.3 – 5.7 4.6 4.5 5.0
Std Deviation (1e-4) 0.3 0.8 – 1.3 1.9 1.5 1.8 1.7 1.2 1.4 1.4 1.6 0.8 1.0 0.9 1.3 – 1.8 1.4 1.0 1.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0124 -0.0743 0.0189 -0.5765 -0.6974 -0.2224 -0.1169 -0.0075 0.154 0.2378 -0.4023 -0.3495 -0.7086 -0.5531 -0.8072 -0.3674 -0.4139 -0.5033
Actin 0.0167 0.0136 0.0092 0.0055 0.0442 0.0077 0.0346 0.0148 0.0269 0.0093 0.0382 0.0024 0.0354 0.0175 0.0337 0.0026 0.0643 0.0174
Bud 0.0018 0.0116 0.0021 0.0013 0.0033 0.0011 0.0016 0.0055 0.0037 0.002 0.0028 0.0009 0.001 0.0054 0.0032 0.0036 0.0014 0.0011
Bud Neck 0.0038 0.0014 0.0018 0.0005 0.0044 0.0167 0.0041 0.0022 0.0013 0.0027 0.0019 0.0313 0.0072 0.0015 0.0015 0.0008 0.0007 0.0062
Bud Periphery 0.0038 0.0126 0.0069 0.0069 0.0105 0.0035 0.0043 0.0068 0.0093 0.0039 0.0137 0.0023 0.0021 0.0097 0.007 0.0174 0.0024 0.0026
Bud Site 0.0106 0.0449 0.0202 0.0009 0.0077 0.0016 0.0126 0.0514 0.0128 0.0103 0.0035 0.0053 0.0108 0.0475 0.0097 0.0058 0.0014 0.0044
Cell Periphery 0.0009 0.0026 0.0041 0.0004 0.0027 0.0012 0.0015 0.0037 0.0028 0.0007 0.0005 0.001 0.0021 0.0021 0.0036 0.0045 0.0004 0.0014
Cytoplasm 0.0357 0.1021 0.0756 0.0136 0.0319 0.0667 0.0386 0.1037 0.0821 0.0239 0.0468 0.0678 0.0426 0.1016 0.0666 0.0254 0.1189 0.081
Cytoplasmic Foci 0.1152 0.1096 0.0676 0.0982 0.1109 0.122 0.122 0.1429 0.1223 0.0623 0.0809 0.1067 0.0841 0.1225 0.0692 0.0475 0.0644 0.0863
Eisosomes 0.0009 0.0007 0.0008 0.0004 0.0008 0.0009 0.0012 0.0014 0.0018 0.0005 0.0003 0.0005 0.005 0.0006 0.0011 0.0007 0.0016 0.0009
Endoplasmic Reticulum 0.0043 0.0054 0.0058 0.0012 0.0032 0.0036 0.0076 0.0053 0.0038 0.0013 0.0021 0.0028 0.0184 0.0126 0.0053 0.0067 0.0123 0.0057
Endosome 0.1921 0.1452 0.0664 0.0652 0.0923 0.0882 0.1602 0.1147 0.1149 0.1383 0.1804 0.1002 0.1658 0.1527 0.1542 0.142 0.1852 0.1486
Golgi 0.0563 0.0403 0.0247 0.0331 0.0285 0.0464 0.0463 0.0574 0.0417 0.0488 0.0896 0.0391 0.0648 0.0698 0.0502 0.0463 0.1038 0.0613
Lipid Particles 0.0439 0.0273 0.05 0.0185 0.0474 0.0335 0.0865 0.0156 0.0217 0.0209 0.053 0.012 0.0349 0.0269 0.015 0.0025 0.026 0.0303
Mitochondria 0.264 0.2853 0.3984 0.4924 0.3781 0.3829 0.1831 0.2523 0.3532 0.3636 0.3429 0.4695 0.3389 0.2757 0.452 0.5654 0.2573 0.4173
None 0.1624 0.1469 0.1937 0.1649 0.0969 0.1212 0.221 0.1477 0.1501 0.1717 0.0911 0.1017 0.0985 0.0825 0.0675 0.106 0.0845 0.0807
Nuclear Periphery 0.009 0.0013 0.0058 0.0004 0.0013 0.0036 0.007 0.0027 0.0016 0.0008 0.0018 0.001 0.0141 0.0021 0.0041 0.0011 0.0215 0.001
Nucleolus 0.0054 0.002 0.0062 0.0002 0.0007 0.0066 0.0021 0.0016 0.0012 0.0012 0.0007 0.0009 0.0016 0.0012 0.001 0.0003 0.0018 0.0012
Nucleus 0.0063 0.0021 0.0051 0.0004 0.0008 0.0031 0.0029 0.0026 0.0019 0.001 0.0075 0.0025 0.004 0.0014 0.0038 0.0009 0.0144 0.0014
Peroxisomes 0.0369 0.0185 0.028 0.0872 0.114 0.0724 0.0363 0.0506 0.0287 0.1135 0.0246 0.041 0.0313 0.0428 0.032 0.0075 0.0149 0.0325
Punctate Nuclear 0.0106 0.0018 0.0089 0.0019 0.0018 0.0061 0.0095 0.0015 0.0037 0.0009 0.0064 0.0026 0.008 0.0013 0.0016 0.0006 0.0059 0.0015
Vacuole 0.0128 0.0196 0.0133 0.0031 0.0141 0.0071 0.0121 0.0124 0.011 0.0146 0.0059 0.0049 0.02 0.0175 0.0118 0.0064 0.0107 0.0108
Vacuole Periphery 0.0065 0.005 0.0055 0.004 0.0046 0.0038 0.0046 0.0031 0.0036 0.0077 0.0053 0.0035 0.0093 0.0052 0.0059 0.006 0.0064 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3845 6.9956 8.8989 10.0437 13.7698 11.1732 11.1151 10.5709 9.4339 11.5304
Translational Efficiency 0.826 0.8234 0.7803 0.6367 0.4786 0.8972 0.8335 0.946 0.9491 0.6673

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1555 316 238 1628 918 1866 186 824 2473 2182 424 2452

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.97 772.49 793.60 787.08 638.45 738.24 925.67 859.71 670.22 743.20 851.54 811.49
Standard Deviation 81.63 105.10 119.77 102.35 81.55 99.21 99.89 106.50 85.17 100.80 129.32 109.29
Intensity Change Log 2 — 0.165075 0.203971 0.192069 — 0.209516 0.535924 0.429276 — 0.186621 0.373171 0.311032

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000996 0.002349 0.011899 0.009396 0.001173 0.002507 0.005618 0.018109 0.001062 0.002484 0.009144 0.012324
Bud Neck 0.034567 0.029585 0.043905 0.039394 0.033621 0.025806 0.009847 0.030032 0.034216 0.026354 0.028964 0.036248
Bud Site 0.015699 0.037528 0.104149* 0.079471 0.025286 0.029651 0.018024 0.103009* 0.019258 0.030792 0.066368 0.087381
Cell Periphery 0.000273 0.000344 0.000369 0.000272 0.000528 0.000279 0.000385 0.000300 0.000368 0.000288 0.000376 0.000281
Cytoplasm 0.128762* 0.031980 0.093277 0.198213* 0.098391 0.038185 0.242056* 0.247662* 0.117488* 0.037286 0.158544* 0.214831*
Cytoplasmic Foci 0.349567* 0.188784* 0.140671 0.144297 0.305232* 0.178671 0.140784 0.141936 0.333109* 0.180135* 0.140721 0.143504
Eisosomes 0.000286 0.000240 0.000222 0.000207 0.000347 0.000216 0.000239 0.000228 0.000308 0.000220 0.000230 0.000214
Endoplasmic Reticulum 0.002517 0.001617 0.011217 0.004652 0.001501 0.000743 0.009950 0.004330 0.002140 0.000869 0.010661 0.004544
Endosome 0.069596 0.088042 0.046822 0.029546 0.072375 0.092157 0.033518 0.023119 0.070628 0.091561 0.040986 0.027386
Golgi 0.052416 0.118213* 0.019152 0.016090 0.059687 0.106067 0.002060 0.011615 0.055115 0.107826 0.011654 0.014587
Lipid Particles 0.027594 0.008844 0.008102 0.005769 0.023115 0.007714 0.014921 0.003677 0.025931 0.007878 0.011093 0.005066
Mitochondria 0.162978* 0.304630* 0.035618 0.048129 0.237778* 0.370106* 0.001006 0.009394 0.190745* 0.360624* 0.020434 0.035112
Mitotic Spindle 0.003899 0.022547 0.066583 0.059687 0.006219 0.013505 0.007846 0.035038 0.004760 0.014815 0.040816 0.051404
None 0.001947 0.001374 0.001682 0.000707 0.001950 0.000414 0.001956 0.000926 0.001948 0.000553 0.001802 0.000780
Nuclear Periphery 0.000470 0.000312 0.001607 0.001243 0.000624 0.000142 0.002395 0.000656 0.000527 0.000166 0.001952 0.001045
Nuclear Periphery Foci 0.000338 0.000415 0.001756 0.001048 0.000383 0.000207 0.007279 0.001041 0.000355 0.000237 0.004179 0.001045
Nucleolus 0.001610 0.000688 0.003339 0.002228 0.002197 0.000833 0.002986 0.000744 0.001828 0.000812 0.003184 0.001729
Nucleus 0.031698 0.005921 0.144235* 0.102702 0.021921 0.006282 0.249918* 0.093714 0.028069 0.006230 0.190596* 0.099682
Peroxisomes 0.046369 0.112981* 0.146087* 0.093989 0.063508 0.075746 0.041210 0.096849 0.052732 0.081138 0.100080* 0.094950
Vacuole 0.054370 0.029458 0.113683 0.157681 0.031590 0.038919 0.205398* 0.175720 0.045914 0.037548 0.153917 0.163743
Vacuole Periphery 0.014047 0.014149 0.005624 0.005278 0.012575 0.011851 0.002603 0.001902 0.013500 0.012183 0.004299 0.004144

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.83 -6.23 -7.28 -5.53 1.87 -2.76 -2.96 -6.76 -5.99 -4.32 -3.76 -6.83 -9.92 -7.95 -1.04
Bud Neck 0.98 -1.87 -0.78 -1.45 1.52 2.40 6.99 2.32 -0.01 -5.59 3.31 1.20 0.52 -3.10 -0.92
Bud Site -4.84 -8.54 -14.84 -5.31 3.34 -1.43 2.59 -9.31 -9.04 -11.21 -5.76 -7.31 -17.11 -12.35 -0.91
Cell Periphery -1.35 -2.08 1.18 2.53 3.55 2.72 1.42 2.81 0.21 3.19 1.76 -0.36 3.02 1.61 4.96
Cytoplasm 14.60 3.44 -10.31 -23.70 -10.62 10.50 -6.41 -14.32 -23.13 -2.00 19.72 -2.77 -16.88 -35.34 -7.22
Cytoplasmic Foci 13.62 20.22 32.78 5.87 0.72 15.32 14.73 21.14 9.17 0.03 26.00 25.22 38.73 11.77 0.46
Eisosomes 0.79 2.23 4.19 0.90 1.94 2.95 2.09 3.09 0.47 2.06 3.85 3.01 5.09 1.54 2.83
Endoplasmic Reticulum 1.69 -6.50 -5.57 -5.89 4.99 2.48 -8.28 -6.66 -10.10 5.63 5.58 -9.92 -8.38 -15.47 7.29
Endosome -2.53 4.23 14.53 9.27 3.13 -4.12 8.73 13.81 20.58 2.98 -5.76 8.12 19.80 22.55 3.95
Golgi -7.54 8.88 14.64 12.61 1.41 -9.62 17.34 14.28 25.88 -4.00 -13.91 16.69 20.20 28.86 -0.17
Lipid Particles 6.96 9.93 12.67 1.77 2.55 7.44 3.16 9.63 5.05 5.80 12.44 9.18 15.83 4.11 5.95
Mitochondria -7.27 14.70 18.91 14.42 -0.72 -10.91 24.88 24.14 46.82* -5.86 -19.22 26.69 29.41 45.60* -2.04
Mitotic Spindle -5.08 -5.94 -14.20 -6.46 0.71 -4.01 -0.28 -6.77 -4.89 -6.17 -7.48 -5.77 -15.60 -11.12 -1.49
None 0.49 0.52 2.70 0.62 1.25 3.54 0.56 2.26 -4.30 1.78 3.99 0.73 3.51 -1.71 1.95
Nuclear Periphery 0.94 -2.97 -8.05 -6.45 -0.31 1.70 -5.74 -1.20 -9.97 7.61 3.18 -5.92 -6.36 -12.20 2.22
Nuclear Periphery Foci -0.40 -6.23 -8.67 -3.94 2.71 2.33 -5.91 -8.70 -11.23 5.14 2.14 -7.10 -11.72 -12.13 5.54
Nucleolus 3.52 -2.35 -1.21 -4.01 1.60 3.71 -0.58 4.14 0.07 2.79 4.41 -2.27 0.66 -3.35 2.58
Nucleus 9.95 -7.91 -17.70 -23.53 2.21 6.01 -12.23 -14.52 -18.21 7.12 12.53 -13.87 -22.87 -30.31 6.40
Peroxisomes -6.50 -6.32 -6.63 3.48 4.03 -2.30 4.17 -0.94 0.92 -4.55 -7.33 -3.89 -5.66 1.59 1.42
Vacuole 5.11 -6.84 -25.92 -26.58 -5.73 -2.49 -13.76 -24.31 -23.53 0.99 3.16 -13.99 -35.48 -36.87 -2.58
Vacuole Periphery -0.03 5.37 8.82 3.56 0.73 0.52 8.32 10.23 13.97 0.69 1.20 8.88 12.50 11.13 0.62
Standard name
Human Ortholog
Description Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
Localization
Cell Percentages mitochondrion (73%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Lip2

Lip2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lip2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available