Standard name | |
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Human Ortholog | |
Description | Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | – | – | – | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Bud | 6 | 2 | 0 | 0 | 1 | 3 | 0 | – | 2 | 3 | 1 | 2 | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 5 | 4 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 133 | 129 | 9 | 19 | 43 | 16 | 56 | – | 10 | 19 | 30 | 32 | – | – | – | 273 | 156 | 165 | 23 | 20 | 11 | 0 | 0 | 0 |
Cytoplasm | 277 | 155 | 42 | 69 | 77 | 84 | 194 | – | 52 | 88 | 63 | 86 | – | – | – | 288 | 200 | 203 | 246 | 140 | 49 | 8 | 9 | 33 |
Endoplasmic Reticulum | 7 | 3 | 1 | 2 | 3 | 1 | 4 | – | 0 | 3 | 1 | 2 | – | – | – | 7 | 6 | 10 | 13 | 7 | 5 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 1 | 0 | 0 | 1 | 4 | – | 0 | 4 | 3 | 3 | – | – | – | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2 | 0 | 0 | 3 | 0 | 3 | 2 | – | 1 | 0 | 2 | 2 | – | – | – | 24 | 20 | 10 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 13 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Unique Cell Count | 290 | 175 | 44 | 77 | 81 | 86 | 203 | 55 | 98 | 73 | 98 | 377 | 243 | 244 | 298 | 181 | 79 | 13 | 11 | 39 | ||||
Labelled Cell Count | 427 | 291 | 53 | 94 | 124 | 108 | 260 | 65 | 120 | 101 | 127 | 613 | 385 | 390 | 298 | 181 | 79 | 13 | 11 | 39 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 12.7 | 9.6 | 9.3 | 11.2 | 9.1 | 9.7 | – | 8.6 | 8.8 | 8.7 | 8.3 | – | – | – | 16.5 | 16.0 | 15.5 | – | – | – |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.4 | 2.2 | 1.6 | 1.2 | 1.6 | – | 1.3 | 1.7 | 1.6 | 1.1 | – | – | – | 2.9 | 3.0 | 3.2 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.05 | 0.22 | -0.08 | 0.01 | – | -0.16 | -0.13 | -0.14 | -0.21 | – | – | – | 0.78 | 0.74 | 0.69 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0.5 | 3.5 | -0.3 | 1.0 | – | -0.3 | -0.1 | 2.3 | 1.5 | – | – | – | 6.9 | 5.4 | 5.9 |
Cytoplasm | -1.1 | -0.1 | 0.7 | 0 | – | -0.2 | -1.1 | -1.6 | -1.4 | – | – | – | -2.9 | -2.2 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.9664 | 8.0871 | 6.926 | 6.0162 | 6.0114 | 7.2462 | 6.6186 | 8.3864 | 7.5094 | 6.7553 | 7.2105 | 7.9311 | 8.3322 | 10.0009 | 8.9459 | 8.4255 | 8.4752 | 8.9156 |
Actin | 0.0259 | 0.0027 | 0.019 | 0.0037 | 0.0159 | 0.004 | 0.03 | 0.0022 | 0.005 | 0.0031 | 0.0071 | 0.0022 | 0.0389 | 0.0024 | 0.0191 | 0.0095 | 0.0222 | 0.0031 |
Bud | 0.0017 | 0.0082 | 0.0052 | 0.0006 | 0.0014 | 0.0019 | 0.0006 | 0.0004 | 0.0015 | 0.0005 | 0.0115 | 0.0001 | 0.0008 | 0.0001 | 0.0019 | 0.0006 | 0.0019 | 0.0001 |
Bud Neck | 0.0071 | 0.0007 | 0.0022 | 0.0044 | 0.0044 | 0.0101 | 0.0136 | 0.001 | 0.0018 | 0.0033 | 0.0023 | 0.0043 | 0.0023 | 0.001 | 0.0041 | 0.0029 | 0.0073 | 0.0063 |
Bud Periphery | 0.0044 | 0.0029 | 0.0037 | 0.0029 | 0.0057 | 0.0089 | 0.0042 | 0.0012 | 0.0032 | 0.0023 | 0.0125 | 0.0011 | 0.0027 | 0.0003 | 0.0056 | 0.002 | 0.0024 | 0.0009 |
Bud Site | 0.0102 | 0.0251 | 0.0079 | 0.0016 | 0.0043 | 0.0012 | 0.0054 | 0.0136 | 0.0067 | 0.0057 | 0.0072 | 0.0008 | 0.0054 | 0.0066 | 0.0133 | 0.0021 | 0.0035 | 0.0003 |
Cell Periphery | 0.0885 | 0.0226 | 0.0569 | 0.0923 | 0.0614 | 0.0593 | 0.1299 | 0.0447 | 0.0745 | 0.061 | 0.08 | 0.0873 | 0.1101 | 0.0457 | 0.0624 | 0.0584 | 0.0385 | 0.0389 |
Cytoplasm | 0.7019 | 0.929 | 0.8692 | 0.8409 | 0.7313 | 0.8683 | 0.7377 | 0.9278 | 0.8818 | 0.8762 | 0.8655 | 0.8717 | 0.7557 | 0.9267 | 0.8591 | 0.7861 | 0.854 | 0.9171 |
Cytoplasmic Foci | 0.0208 | 0.0006 | 0.0052 | 0.0091 | 0.0296 | 0.0064 | 0.0035 | 0.0005 | 0.0008 | 0.0017 | 0.0003 | 0.0018 | 0.0069 | 0.0007 | 0.0029 | 0.0058 | 0.0068 | 0.0004 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.001 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0226 | 0.0047 | 0.0043 | 0.0055 | 0.0056 | 0.0064 | 0.0306 | 0.0051 | 0.0084 | 0.0264 | 0.0102 | 0.0095 | 0.0334 | 0.0125 | 0.0131 | 0.0064 | 0.0152 | 0.0179 |
Endosome | 0.031 | 0.0002 | 0.0065 | 0.0044 | 0.0426 | 0.0099 | 0.0033 | 0.0001 | 0.0008 | 0.0008 | 0.0001 | 0.0011 | 0.0053 | 0.0002 | 0.002 | 0.0039 | 0.0102 | 0.0021 |
Golgi | 0.0137 | 0.0002 | 0.0065 | 0.0136 | 0.0187 | 0.0087 | 0.0034 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0008 | 0.0068 | 0.0004 | 0.0007 | 0.0016 | 0.0176 | 0.0012 |
Lipid Particles | 0.0123 | 0.0003 | 0.0031 | 0.0033 | 0.026 | 0.0063 | 0.006 | 0.0005 | 0.0009 | 0.0042 | 0.0005 | 0.0038 | 0.0074 | 0.001 | 0.0026 | 0.0066 | 0.0026 | 0.0015 |
Mitochondria | 0.0092 | 0.0001 | 0.0029 | 0.0029 | 0.0259 | 0.0033 | 0.0108 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0037 | 0 | 0.0001 | 0.0405 | 0.0097 | 0.0005 |
None | 0.0101 | 0.001 | 0.001 | 0.0004 | 0.0016 | 0.0008 | 0.0037 | 0.0009 | 0.0032 | 0.0065 | 0.0004 | 0.0016 | 0.0045 | 0.0003 | 0.0017 | 0.0016 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.0063 | 0.0002 | 0.0002 | 0.0003 | 0.005 | 0.0005 | 0.0015 | 0.0002 | 0.0017 | 0.0009 | 0.0003 | 0.0037 | 0.0026 | 0.0003 | 0.001 | 0.0222 | 0.0007 | 0.0069 |
Nucleolus | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0014 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Nucleus | 0.0025 | 0.0004 | 0.0005 | 0.0004 | 0.0008 | 0.0004 | 0.0015 | 0.0003 | 0.0047 | 0.0012 | 0.0003 | 0.0006 | 0.0012 | 0.0002 | 0.0005 | 0.0103 | 0.0015 | 0.0003 |
Peroxisomes | 0.0172 | 0 | 0.0013 | 0.0029 | 0.0115 | 0.0008 | 0.0038 | 0 | 0 | 0 | 0 | 0.0049 | 0.0008 | 0 | 0.0003 | 0.0306 | 0.0031 | 0 |
Punctate Nuclear | 0.0025 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0002 | 0.001 | 0 | 0.0005 | 0.0004 | 0.0001 | 0.0025 | 0.0023 | 0 | 0.0028 | 0.0008 | 0.0003 | 0.0002 |
Vacuole | 0.0084 | 0.0011 | 0.0038 | 0.0106 | 0.005 | 0.0023 | 0.0066 | 0.0011 | 0.0032 | 0.0046 | 0.0014 | 0.0018 | 0.0071 | 0.0015 | 0.0064 | 0.006 | 0.0016 | 0.0017 |
Vacuole Periphery | 0.0022 | 0 | 0.0005 | 0.0001 | 0.0011 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.001 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 73.4399 | 63.4983 | 67.0839 | 76.0384 | 63.6088 | 66.1796 | 54.0013 | 67.7533 | 78.2092 | 58.8225 |
Translational Efficiency | 1.6298 | 1.3498 | 1.2947 | 1.3524 | 1.1292 | 1.6561 | 1.3984 | 1.1098 | 1.1988 | 1.3224 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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