Standard name
Human Ortholog
Description R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.14 0.09 0.1 0 0.05 0 0 – 0 0 0 0.21 0.13 0.11 0.33 0.27 0.26 0.12 0.12 0.1 0.09 0.14 0.15
Bud 0.07 0 0.06 0 0.09 0.05 0.08 0.09 – 0.06 0.12 0.12 0 0 0 0.08 0.1 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.53 0.41 0.57 0.49 0.39 0.32 0.23 – 0.25 0.24 0.25 0.39 0.41 0.52 0.42 0.42 0.35 0 0.14 0.05 0 0 0.05
Endoplasmic Reticulum 0.09 0.25 0 0.19 0.09 0.1 0.13 0.06 – 0.1 0.05 0.06 0.23 0.11 0.08 0 0.05 0 0.06 0.11 0.12 0.05 0.08 0.12
Endosome 0.38 0.07 0.28 0.06 0.22 0.16 0.1 0.13 – 0 0.05 0.07 0.22 0.29 0.19 0.1 0.13 0.23 0.33 0.24 0.24 0.23 0.2 0.21
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0 0.21 0.08 0.11 0.17 0.16 0.15
Mitochondria 0.16 0.08 0.23 0.14 0.19 0.4 0.48 0.56 – 0.59 0.65 0.62 0 0 0 0.1 0.07 0.05 0.13 0.16 0.23 0.27 0.19 0.18
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0 0 0 0 0.05 0 0.05 – 0.07 0.05 0 0.09 0.09 0.13 0.1 0.18 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0 0.06 0.08 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 28 14 16 5 17 10 20 – 16 9 14 26 24 17 28 16 17 40 36 18 9 33 39
Bud 18 6 9 6 20 18 32 48 – 25 33 49 3 4 6 7 6 4 4 7 2 1 6 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 0 1 3 1 0 3 2
Bud Site 2 0 0 0 1 8 4 5 – 8 3 8 0 0 0 0 2 0 – – – – – –
Cell Periphery 2 0 0 2 2 0 1 3 – 4 1 8 0 2 2 1 0 0 0 0 0 0 0 0
Cytoplasm 54 108 61 92 108 128 121 125 – 99 68 105 49 74 81 36 25 23 7 41 8 2 6 12
Endoplasmic Reticulum 23 51 6 30 20 34 50 33 – 39 13 23 29 19 12 3 3 0 20 30 21 5 20 32
Endosome 94 15 41 10 48 53 39 69 – 16 14 31 28 52 30 9 8 15 112 69 43 25 51 53
Golgi 5 2 0 0 0 0 0 0 – 0 0 0 2 2 1 5 4 1 72 22 19 18 40 40
Mitochondria 39 16 35 22 41 131 185 306 – 232 181 259 4 3 0 9 4 3 44 46 40 29 47 46
Nucleus 0 1 0 1 1 2 2 4 – 1 0 1 0 1 2 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 – 2 0 3 1 0 0 0 0 0 1 1 0 1 0 0
Nucleolus 2 0 0 0 0 5 3 14 – 1 1 5 0 0 1 0 0 0 0 0 0 0 1 0
Peroxisomes 6 0 8 1 8 6 7 11 – 2 1 8 0 4 0 0 1 0 14 4 7 3 9 5
SpindlePole 25 6 6 5 3 16 12 25 – 26 13 14 11 17 20 9 11 7 13 5 7 3 5 4
Vac/Vac Membrane 14 3 3 0 7 5 10 13 – 16 5 5 2 7 2 2 3 4 9 19 6 6 18 13
Unique Cell Count 246 204 149 162 221 329 384 543 393 278 416 125 180 155 86 60 65 346 290 179 109 251 261
Labelled Cell Count 313 237 183 185 264 423 476 677 487 342 533 155 209 175 109 86 75 346 290 179 109 251 261


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.5 5.5 5.6 5.7 5.4 5.1 5.2 – 4.7 5.0 4.9 7.1 7.1 6.7 7.3 7.1 7.7 8.1 8.2 8.6
Std Deviation (1e-4) 0.9 0.7 0.9 1.2 1.4 1.3 1.3 1.1 – 0.9 0.9 0.9 1.1 1.1 0.9 0.9 1.1 1.1 1.4 1.2 1.8
Intensity Change (Log2) – – – 0.03 0.05 -0.02 -0.11 -0.07 – -0.22 -0.14 -0.16 0.38 0.37 0.29 0.42 0.39 0.5 0.56 0.59 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.1 -3.0 -1.7 -3.4 -2.9 – 0 -2.7 0 2.7 1.1 0.5 4.5 3.2 3.2
Bud -1.0 1.1 -0.2 0.9 1.1 – 0 1.9 0 -1.5 -1.8 -0.9 0.6 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 1.5 -0.4 -2.1 -4.4 – -3.6 -3.5 -3.6 -0.3 0 2.0 0.1 0.1 -0.8
Endoplasmic Reticulum 4.0 1.9 2.3 3.0 1.0 – 0 0.3 0 4.7 2.2 1.4 0 0 0
Endosome -5.1 -1.3 -2.9 -5.0 -4.4 – 0 -6.6 0 -1.0 0.3 -1.7 -3.1 -2.2 -0.7
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -2.3 -1.2 3.5 5.2 7.1 – 0 8.2 0 -4.8 -6.2 -6.4 -2.5 -2.8 -3.3
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 -0.8 0 0 0 – 0 0 0 0 -1.5 0 0 0 0
SpindlePole -0.4 0 0.4 -0.5 0.3 – 0 0.3 0 1.6 1.9 2.8 1.9 0 0
Vacuole 0 0 0 0 0 – 1.2 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5588 3.831 3.4785 3.5056 3.4352 3.6796 4.1831 4.5768 5.2154 4.3782 4.2646 4.6812 2.5278 3.0388 2.9268 3.208 2.7465 3.0962
Actin 0.0331 0.0113 0.0267 0.0114 0.0204 0.032 0.0427 0.0285 0.0357 0.01 0.0072 0.0543 0.0276 0.0143 0.0134 0.0031 0.0259 0.0157
Bud 0.0006 0.0008 0.0014 0.0005 0.0006 0.0004 0.0005 0.0013 0.0011 0.0002 0.0002 0.0007 0.0004 0.0007 0.0013 0.0004 0.0021 0.0003
Bud Neck 0.0052 0.001 0.0011 0.0012 0.0006 0.0034 0.0037 0.0009 0.0015 0.0004 0.0008 0.0034 0.0012 0.0007 0.001 0.0009 0.0017 0.0082
Bud Periphery 0.0008 0.0006 0.0011 0.0003 0.0006 0.0003 0.0007 0.0009 0.0013 0.0001 0.0001 0.0007 0.0004 0.0003 0.0011 0.0005 0.0028 0.0002
Bud Site 0.004 0.0085 0.0033 0.0014 0.006 0.0007 0.0025 0.007 0.0053 0.0004 0.0006 0.001 0.0032 0.0073 0.0042 0.0012 0.011 0.0005
Cell Periphery 0.0005 0.0003 0.0002 0.0001 0.0002 0.0002 0.0006 0.0002 0.0004 0 0.0001 0.0002 0.0003 0.0002 0.0003 0.0001 0.0006 0.0001
Cytoplasm 0.065 0.1856 0.0937 0.0811 0.0507 0.0624 0.0792 0.1108 0.1057 0.0531 0.0326 0.0889 0.0527 0.1408 0.1031 0.0777 0.0859 0.0841
Cytoplasmic Foci 0.1851 0.2253 0.2178 0.2742 0.1719 0.2417 0.2394 0.2411 0.2065 0.2538 0.2176 0.2068 0.2385 0.2626 0.2328 0.2226 0.2566 0.2793
Eisosomes 0.0007 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0 0.0002 0.0004 0.0001 0.0003 0.0001 0.0001 0
Endoplasmic Reticulum 0.0307 0.0159 0.0129 0.0057 0.0159 0.0169 0.0137 0.0268 0.0263 0.0053 0.0581 0.0367 0.0287 0.0228 0.0243 0.0067 0.0029 0.0112
Endosome 0.3645 0.3215 0.3619 0.3132 0.4437 0.336 0.2588 0.2586 0.2699 0.2782 0.3832 0.2567 0.36 0.3356 0.3568 0.3652 0.3496 0.3169
Golgi 0.1562 0.1481 0.2152 0.2427 0.1889 0.2058 0.2205 0.21 0.2343 0.3316 0.204 0.2092 0.1761 0.1389 0.1722 0.1333 0.176 0.1868
Lipid Particles 0.0393 0.0243 0.0178 0.024 0.0382 0.036 0.0366 0.0127 0.0106 0.0109 0.0186 0.0186 0.0362 0.0139 0.0175 0.0147 0.0146 0.0181
Mitochondria 0.0217 0.011 0.0128 0.0072 0.0278 0.01 0.0048 0.0072 0.0133 0.0061 0.0063 0.0277 0.0211 0.0082 0.0158 0.0511 0.0055 0.0144
None 0.0237 0.0136 0.0028 0.0014 0.0037 0.008 0.0407 0.0377 0.0587 0.0005 0.0007 0.0347 0.0105 0.0208 0.0109 0.0425 0.0256 0.0163
Nuclear Periphery 0.0124 0.0022 0.0018 0.0006 0.0032 0.003 0.0026 0.0022 0.0014 0.0005 0.0013 0.0021 0.0041 0.0021 0.0023 0.0252 0.0008 0.001
Nucleolus 0.0013 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0 0.0001 0.0004 0.0003 0.0003 0.0004 0.0002 0.0001
Nucleus 0.0107 0.0009 0.0005 0.0002 0.001 0.0005 0.0004 0.0005 0.0005 0.0001 0.0002 0.0004 0.0006 0.0018 0.0007 0.0032 0.0005 0.0005
Peroxisomes 0.0228 0.0208 0.0216 0.0276 0.0144 0.0359 0.0473 0.0471 0.0196 0.0461 0.0637 0.0522 0.0272 0.0126 0.0308 0.0388 0.0309 0.0398
Punctate Nuclear 0.0116 0.0013 0.0009 0.0042 0.004 0.0019 0.0014 0.001 0.002 0.0005 0.0006 0.0011 0.0041 0.0095 0.0032 0.0028 0.0019 0.0011
Vacuole 0.0066 0.005 0.0044 0.0017 0.0052 0.0029 0.0022 0.0035 0.0041 0.0011 0.0025 0.0025 0.0044 0.0047 0.0054 0.0063 0.0032 0.0033
Vacuole Periphery 0.0036 0.0016 0.0021 0.0012 0.0027 0.0016 0.0012 0.0017 0.0014 0.001 0.0015 0.0018 0.0019 0.0018 0.0024 0.0033 0.0014 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.4533 44.3487 29.5043 50.9488 52.1231 41.0342 39.8156 46.9623 37.8604 48.1123
Translational Efficiency 1.4464 2.2012 2.2691 1.302 1.3085 2.1654 1.9578 1.7534 2.0082 1.466

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
Localization
Cell Percentages Golgi (9%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec22

Sec22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available