Standard name
Human Ortholog
Description Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes; required for synthesis of wild-type levels of Coenzyme Q (ubiquinone); member of the short-chain dehydrogenase/reductase (SDR) superfamily; orthologous gene in some other fungi is fused to the COQ10 ortholog

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.07 0 0 0 0 0.06 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.07 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.14 0.1 0.22 0 0 0 0.06 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Endosome 0.08 0 0.11 0.06 0.22 0.12 0.16 0.12 0 0.06 0 0 0.14 0.19 0.32 0.09 0.15 0.12 0.11 0.08 0.08 0.09 0.15 0.13
Golgi 0 0 0.15 0 0.07 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0.2 0.17 0 0.1 0.06 0.06 0.07 0.08
Mitochondria 0.92 0.96 0.67 0.89 0.5 0.76 0.69 0.69 0.9 0.87 0.93 0.84 0.74 0.53 0.34 0 0.05 0.11 0.74 0.62 0.69 0.58 0.51 0.38
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0.15 0.06 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0 0.05 0.08 0.05 0.07 0.05 0.07 0.06 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.09 0.13 0 0.06 0 0 0 0 0.05 0.11 0.22 0.29 0.27 0.15 0.2 0.08 0.06 0.11 0.08 0.12 0.19
SpindlePole 0 0 0.16 0.05 0.2 0.11 0.1 0.12 0.05 0.05 0 0.06 0.12 0.2 0.24 0.05 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.08 0.09 0 0.11 0.05 0.08 0.07 0 0 0 0 0.06 0 0 0.23 0.17 0.07 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 4 6 0 0 3 0 2 0 0 6 7 3 0 0 0 0 2 7 2 6 10
Bud 1 1 8 17 11 14 10 17 16 26 3 10 4 12 9 0 1 2 0 0 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 1 0 1 1 0 0 0 0 1 0 0 2 2
Bud Site 0 0 0 0 0 1 1 4 1 4 0 4 0 0 0 0 0 1
Cell Periphery 1 1 12 7 3 1 5 4 1 3 1 0 5 1 8 4 3 2 0 0 0 0 0 1
Cytoplasm 1 0 8 6 15 14 20 21 6 15 4 8 9 10 12 3 4 18 0 0 0 8 13 20
Endoplasmic Reticulum 1 0 4 1 2 1 1 1 1 1 0 1 5 6 0 2 1 0 0 0 0 1 0 2
Endosome 6 0 40 16 94 48 85 62 3 27 0 8 27 45 83 2 6 10 10 6 35 12 55 36
Golgi 0 0 52 6 28 4 5 7 0 5 0 0 7 13 18 1 8 14 2 8 24 8 24 24
Mitochondria 72 49 240 231 212 291 376 364 259 421 112 217 143 125 87 0 2 9 74 49 293 83 186 112
Nucleus 0 0 2 0 4 8 12 10 6 1 2 4 1 1 1 0 0 1 0 0 0 1 1 0
Nuclear Periphery 3 0 2 3 4 15 22 25 16 21 3 11 1 3 2 0 6 5 0 0 4 2 1 1
Nucleolus 8 2 0 3 13 20 43 29 20 26 8 15 0 2 2 0 2 7 0 0 1 0 3 3
Peroxisomes 1 1 41 24 53 16 32 17 9 21 1 12 22 53 74 6 6 16 7 4 48 11 42 54
SpindlePole 0 1 56 12 84 41 56 61 13 22 3 16 24 47 61 1 1 5 0 0 1 2 4 8
Vac/Vac Membrane 10 4 33 10 45 21 42 35 9 17 1 9 11 10 10 5 7 6 1 4 4 5 11 8
Unique Cell Count 78 51 357 261 422 384 544 530 287 486 121 259 194 238 258 22 41 81 100 80 427 144 365 292
Labelled Cell Count 104 59 501 340 574 495 710 661 360 614 138 316 265 336 371 24 47 96 100 80 427 144 365 292


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.8 6.5 6.0 6.9 5.7 6.0 6.0 5.0 5.6 4.8 5.0 5.9 6.2 5.7 7.2 9.1 9.5 8.7 7.8 8.2
Std Deviation (1e-4) 0.8 0.7 1.3 1.2 1.5 1.3 1.5 1.4 1.1 1.3 1.1 1.3 1.7 1.7 1.3 2.1 2.1 2.2 5.9 4.1 5.7
Intensity Change (Log2) -0.11 0.07 -0.19 -0.13 -0.12 -0.38 -0.22 -0.45 -0.38 -0.15 -0.08 -0.2 0.14 0.49 0.54 0.42 0.25 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6279 6.1762 5.3595 4.9299 4.972 5.2642 4.0169 4.5611 4.8227 4.8319 5.2925 5.2828 1.9075 2.7308 2.049 2.4169 2.6157 2.5415
Actin 0.0256 0.0043 0.0168 0.0014 0.0108 0.0058 0.0161 0.0101 0.0527 0.001 0.0023 0.0092 0.0749 0.0449 0.0195 0.0194 0.0955 0.0269
Bud 0.0003 0.0003 0.0002 0 0.0009 0.0001 0.0003 0.0113 0.0026 0.0001 0.0003 0.0001 0.0008 0.0005 0.0003 0.0007 0.0022 0.0001
Bud Neck 0.0005 0.0001 0.0006 0.0001 0.0003 0.0001 0.0002 0.0025 0.001 0.0001 0.0003 0.0003 0.0017 0.0015 0.0003 0.0003 0.0022 0.0002
Bud Periphery 0.0005 0.0004 0.0004 0.0001 0.0017 0.0002 0.0007 0.0111 0.0043 0.0002 0.0006 0.0003 0.0014 0.0005 0.0005 0.0007 0.0021 0.0003
Bud Site 0.0019 0.0004 0.002 0.0001 0.0015 0.0003 0.0008 0.0534 0.0054 0.0001 0.0013 0.0003 0.0034 0.0155 0.0052 0.0047 0.0153 0.0002
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0004 0.0002 0 0.0002 0.0001 0.0006 0.0002 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0017 0.0003 0.0006 0.0001 0.0025 0 0.0026 0.0025 0.0032 0.0001 0.0003 0.0002 0.0082 0.0003 0.0016 0.0022 0.0012 0.0001
Cytoplasmic Foci 0.0418 0.0222 0.0134 0.0164 0.0203 0.0064 0.0504 0.0515 0.0589 0.0075 0.0203 0.0133 0.0391 0.0241 0.0203 0.0326 0.0262 0.0095
Eisosomes 0.0006 0.0002 0.0006 0.0001 0.0002 0.0002 0.0007 0.0001 0.0006 0.0001 0.0003 0.0003 0.0011 0.0005 0.0005 0.0002 0.0005 0.0004
Endoplasmic Reticulum 0.0004 0.0001 0.0009 0 0.0027 0 0.0032 0.0001 0.0014 0.0001 0.0003 0.0001 0.0067 0.0002 0.0003 0.0004 0.0008 0.0002
Endosome 0.0418 0.0245 0.0239 0.0123 0.0645 0.0107 0.0956 0.0263 0.0339 0.0224 0.0228 0.0184 0.0855 0.0344 0.0439 0.1023 0.0236 0.0361
Golgi 0.062 0.0773 0.0386 0.0366 0.0322 0.0354 0.0599 0.0275 0.0567 0.0744 0.0244 0.0577 0.0831 0.087 0.0701 0.1083 0.0315 0.1032
Lipid Particles 0.1135 0.0255 0.038 0.0182 0.0107 0.0627 0.098 0.0245 0.072 0.0146 0.0556 0.03 0.1066 0.0327 0.0384 0.0087 0.0306 0.0444
Mitochondria 0.4881 0.67 0.7291 0.7714 0.5926 0.7209 0.535 0.2554 0.517 0.77 0.6164 0.6647 0.3828 0.5644 0.6723 0.6573 0.504 0.6609
None 0.0037 0.0002 0.0009 0 0.0014 0.0001 0.0026 0.0003 0.003 0 0.0003 0.0001 0.0181 0.0001 0.0119 0.001 0.0017 0.0002
Nuclear Periphery 0.0006 0.0001 0.0022 0 0.0091 0.0006 0.0043 0.0001 0.0007 0 0.0002 0 0.0154 0.0001 0.0003 0.0007 0.0309 0.0002
Nucleolus 0.0015 0.0001 0.0003 0 0.0005 0.0006 0.0004 0.0011 0.0004 0.0001 0.0005 0.0001 0.0035 0.0001 0.0002 0.0006 0.0017 0.0001
Nucleus 0.0004 0.0001 0.0002 0 0.0019 0.0002 0.0007 0.0007 0.0002 0 0.0002 0 0.0063 0.0001 0.0003 0.0007 0.0062 0.0001
Peroxisomes 0.1997 0.1718 0.1246 0.1422 0.1807 0.1528 0.117 0.5086 0.1824 0.1077 0.2468 0.2032 0.1357 0.1896 0.1093 0.0477 0.2098 0.1135
Punctate Nuclear 0.0109 0.0001 0.0004 0 0.0471 0.0001 0.0066 0.0004 0.0012 0 0.0008 0.0001 0.0105 0.0001 0.0003 0.0033 0.0102 0.0002
Vacuole 0.0017 0.0007 0.002 0.0002 0.0114 0.0006 0.0021 0.0106 0.001 0.0005 0.0026 0.0005 0.0071 0.0013 0.0016 0.0048 0.0012 0.0008
Vacuole Periphery 0.0024 0.0011 0.0041 0.0004 0.0067 0.002 0.0025 0.0015 0.001 0.001 0.0034 0.0011 0.0076 0.002 0.0026 0.0031 0.0023 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9227 9.4616 8.7762 14.1446 10.2922 21.1567 16.1437 16.1421 14.3243 16.5999
Translational Efficiency 1.4222 1.8797 1.3104 0.725 1.2432 0.7796 1.1115 1.0856 1.302 0.9483

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1633 815 1528 1850 1544 1415 2466 1493 3177 2230 3994 3343

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.37 920.20 895.66 822.40 782.79 852.50 873.66 865.14 731.17 877.24 882.08 841.49
Standard Deviation 88.36 128.21 116.73 111.83 100.48 109.99 107.43 110.18 106.95 121.44 111.59 113.11
Intensity Change Log 2 0.431393 0.392397 0.269286 0.123075 0.158447 0.144308 0.274890 0.272137 0.203863

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001746 0.004825 0.008431 0.010409 0.002630 0.004041 0.003293 0.015425 0.002176 0.004327 0.005259 0.012649
Bud Neck 0.006621 0.002377 0.007266 0.039374 0.001228 0.001531 0.020825 0.023119 0.004000 0.001840 0.015638 0.032114
Bud Site 0.010762 0.005181 0.023005 0.139947* 0.002806 0.006132 0.060925 0.118054* 0.006895 0.005784 0.046418 0.130170*
Cell Periphery 0.000583 0.000283 0.000183 0.000231 0.000218 0.000328 0.000168 0.000200 0.000406 0.000312 0.000174 0.000217
Cytoplasm 0.002844 0.001458 0.007904 0.090008 0.000978 0.001566 0.034227 0.061136 0.001937 0.001527 0.024157 0.077114
Cytoplasmic Foci 0.038584 0.043763 0.143031 0.123876 0.052515 0.034240 0.127179 0.144680 0.045354 0.037720 0.133243 0.133167
Eisosomes 0.000084 0.000093 0.000100 0.000081 0.000086 0.000091 0.000073 0.000103 0.000085 0.000092 0.000083 0.000091
Endoplasmic Reticulum 0.000255 0.000233 0.000856 0.000599 0.000224 0.000127 0.000834 0.000611 0.000239 0.000166 0.000843 0.000604
Endosome 0.115800 0.068271 0.189650 0.060272 0.096839 0.084998 0.151553 0.085700 0.106585 0.078885 0.166128 0.071628
Golgi 0.077499 0.070971 0.119918* 0.027597 0.098263 0.066282 0.070461 0.054993 0.087590 0.067996 0.089382 0.039832
Lipid Particles 0.010201 0.004385 0.009025 0.002294 0.016365 0.005286 0.003893 0.003914 0.013197 0.004956 0.005856 0.003017
Mitochondria 0.631518* 0.716368* 0.213193* 0.031748 0.655493* 0.710111* 0.138446* 0.055996 0.643170* 0.712398* 0.167043* 0.042577
Mitotic Spindle 0.005727 0.008805 0.122485* 0.164868* 0.004580 0.004512 0.184155* 0.117614* 0.005170 0.006081 0.160562* 0.143764*
None 0.000945 0.000149 0.000192 0.000659 0.000109 0.000146 0.000405 0.000272 0.000539 0.000147 0.000324 0.000486
Nuclear Periphery 0.000058 0.000068 0.000574 0.000264 0.000083 0.000024 0.000379 0.000183 0.000070 0.000040 0.000453 0.000228
Nuclear Periphery Foci 0.000280 0.000102 0.002354 0.000634 0.000242 0.000071 0.001246 0.000700 0.000262 0.000082 0.001670 0.000664
Nucleolus 0.000529 0.000060 0.000869 0.001195 0.000087 0.000169 0.001599 0.001046 0.000314 0.000130 0.001320 0.001128
Nucleus 0.000590 0.000127 0.001938 0.016756 0.000239 0.000108 0.006220 0.007738 0.000419 0.000115 0.004582 0.012728
Peroxisomes 0.085666 0.062377 0.113672* 0.240310* 0.057543 0.074131 0.147850* 0.266348* 0.071999 0.069835 0.134775* 0.251938*
Vacuole 0.005269 0.004834 0.027112 0.046985 0.004275 0.002912 0.039474 0.039487 0.004786 0.003614 0.034744 0.043636
Vacuole Periphery 0.004440 0.005269 0.008244 0.001895 0.005196 0.003195 0.006795 0.002681 0.004807 0.003953 0.007349 0.002246

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.43 -6.05 -5.77 -2.75 -0.26 -2.28 -1.29 -7.14 -6.09 -6.76 -4.26 -5.94 -9.11 -6.45 -5.58
Bud Neck 3.23 -0.72 -14.22 -16.90 -14.62 -0.87 -20.10 -14.51 -14.38 -1.63 3.34 -13.97 -19.80 -22.44 -10.98
Bud Site 3.38 -6.17 -24.84 -26.63* -21.84* -2.92 -24.38* -23.08 -21.99 -9.83 1.06 -22.73 -34.03 -34.36* -20.38
Cell Periphery 3.42 4.81 4.17 1.55 -1.74 -1.85 1.82 0.75 2.41 -1.87 1.65 5.16 4.15 2.61 -2.54
Cytoplasm 2.63 -6.13 -25.42 -25.89 -23.61 -1.96 -17.94 -20.03 -19.85 -9.06 1.31 -18.38 -32.20 -32.42 -21.65
Cytoplasmic Foci -1.51 -24.68 -20.30 -16.47 4.78 6.36 -20.38 -19.56 -24.67 -2.86 3.59 -32.23 -27.86 -29.82 1.34
Eisosomes -1.39 -3.40 -0.64 0.97 2.92 -1.05 2.64 -3.62 -2.52 -6.04 -1.77 -0.02 -3.07 -0.92 -3.22
Endoplasmic Reticulum 0.62 -8.31 -9.05 -9.64 0.79 3.88 -8.24 -7.68 -9.13 1.31 3.46 -11.14 -11.93 -13.85 1.50
Endosome 7.83 -10.60 12.21 2.96 23.52* 2.20 -9.37 3.50 1.23 13.48 6.78 -13.21 11.52 3.96 26.50*
Golgi 1.21 -8.11 15.97 10.83 23.81* 6.76 7.14 11.17 4.64 5.46 5.65 0.34 19.21 12.09 20.89*
Lipid Particles 3.71 0.56 5.17 1.72 5.35 4.73 5.75 5.69 0.67 -0.33 5.71 5.29 7.68 1.60 4.30
Mitochondria -5.74 35.91* 64.86* 57.92* 23.42* -4.31 51.31* 62.25* 67.93* 13.53 -7.25 63.08* 90.27* 91.12* 26.74*
Mitotic Spindle -1.94 -21.42* -25.96* -24.91* -5.23 0.09 -33.96* -21.56 -21.57 8.36 -1.21 -39.82 -33.52 -33.09* 2.55
None 1.94 1.80 0.21 -6.97 -6.24 -1.72 -4.78 -6.10 -4.97 0.37 1.86 0.91 -0.52 -8.23 -4.20
Nuclear Periphery -0.85 -3.92 -12.59 -12.46 0.38 6.59 -6.27 -8.84 -11.64 2.74 4.32 -7.04 -15.22 -16.87 1.47
Nuclear Periphery Foci 3.68 -3.69 -4.74 -5.65 2.25 2.20 -5.88 -3.78 -4.54 0.37 3.95 -5.68 -6.01 -7.23 2.12
Nucleolus 2.27 -1.51 -4.46 -6.76 -1.93 -0.79 -9.37 -4.44 -4.10 0.43 1.50 -6.13 -6.40 -7.47 -1.05
Nucleus 2.17 -3.30 -13.12 -13.44 -10.82 1.01 -8.66 -9.56 -9.68 -2.98 2.44 -9.09 -16.15 -16.47 -9.74
Peroxisomes 3.42 -4.22 -15.50 -17.29 -11.30 -2.94 -15.27 -22.28 -19.44 -11.23 0.49 -13.84 -26.57 -25.68 -15.07
Vacuole 0.50 -12.76 -19.78 -19.81 -12.50 2.29 -19.31 -15.46 -15.88 -4.28 2.38 -22.86 -25.09 -25.58 -11.15
Vacuole Periphery -1.27 -6.04 6.82 5.74 11.03 3.94 -2.40 5.49 1.75 8.01 2.22 -5.77 8.73 5.52 13.56
Standard name
Human Ortholog
Description Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes; required for synthesis of wild-type levels of Coenzyme Q (ubiquinone); member of the short-chain dehydrogenase/reductase (SDR) superfamily; orthologous gene in some other fungi is fused to the COQ10 ortholog
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Coq11

Coq11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available