Standard name
Human Ortholog
Description Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.17 0.15 0.12 0.19 0.19 0.17 0.14 0.23
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0.05 0.13 0.74 0.69 0.73 0.21 0.21 0.07 0.27 0.28 0.27
Mitochondria 0.95 0.92 0.97 0.98 0.94 0.95 – 0.94 0.96 0.97 0.97 0.96 0.94 0.92 0.91 0.08 0.11 0.1 0.59 0.54 0.57 0.46 0.43 0.32
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.08 0.06 0.09 0.11 0.21 – 0.22 0.15 0.16 0.16 0.16 0.07 0.06 0 0.06 0.08 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.07 0.09 0.1
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 – 0.05 0.06 0.05 0 0.05 0 0 0 0.07 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 18 0 4 3 4 – 0 3 4 0 6 8 4 9 27 18 17 84 100 14 30 19 56
Bud 4 1 1 7 2 2 – 1 0 5 6 2 5 0 1 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Bud Site 1 0 0 0 0 0 – 0 0 1 1 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 5 6 0 0 0 0 – 1 3 1 0 0 0 1 1 1 1 2 0 0 0 0 0 0
Cytoplasm 1 5 1 1 3 9 – 5 6 7 3 3 2 4 2 6 4 5 0 0 0 1 0 0
Endoplasmic Reticulum 1 1 0 0 1 3 – 0 0 1 1 0 0 0 0 1 0 8 0 1 0 0 0 0
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 4 0 1 8 2 0 1 0 1
Golgi 26 41 0 1 8 2 – 5 0 6 1 0 16 11 37 116 71 83 145 111 5 50 41 65
Mitochondria 612 453 69 391 345 494 – 705 512 672 469 604 343 207 268 13 11 11 408 286 43 84 62 78
Nucleus 1 1 0 0 0 0 – 0 0 0 1 1 0 0 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 7 3 0 0 1 2 – 6 2 3 0 3 2 0 0 0 0 0 1 1 0 0 1 1
Nucleolus 56 41 4 34 39 111 – 166 80 109 77 100 24 13 11 9 8 4 6 2 0 0 1 6
Peroxisomes 10 0 1 5 4 1 – 1 2 2 0 4 8 2 2 2 2 4 22 17 10 12 13 24
SpindlePole 0 0 0 1 2 0 – 0 0 0 1 1 0 0 0 3 2 3 1 0 0 0 1 0
Vac/Vac Membrane 23 26 1 1 9 9 – 35 30 35 21 32 8 5 7 11 2 8 9 3 0 2 3 2
Unique Cell Count 644 494 71 400 367 520 751 531 696 482 626 365 226 295 157 103 114 696 533 76 184 147 243
Labelled Cell Count 768 596 77 445 417 637 925 638 846 581 756 417 247 338 196 119 147 696 533 76 184 147 243


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 33.0 47.7 36.9 37.2 39.7 37.2 – 44.0 46.4 50.9 49.4 50.0 41.1 42.0 41.4 44.9 39.6 42.3 48.3 50.2 49.8
Std Deviation (1e-4) 9.4 12.4 13.6 11.4 11.1 11.8 – 15.9 19.6 19.8 21.5 21.2 12.3 12.3 12.3 18.2 12.7 17.3 15.4 17.6 17.3
Intensity Change (Log2) – – – 0.01 0.1 0.01 – 0.25 0.33 0.46 0.42 0.44 0.16 0.19 0.16 0.28 0.1 0.19 0.39 0.44 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 3.7 3.7 3.4
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 3.1 10.3 9.1 9.7
Mitochondria 0.3 -1.1 -0.8 – -1.1 -0.3 -0.3 0.1 -0.3 -1.1 -1.6 -1.8 -13.0 -11.3 -11.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 1.3 3.1 – 3.3 2.2 2.3 2.3 2.3 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 58.2968 58.8642 63.3561 55.0596 57.7269 58.1891 45.1445 56.1454 47.875 38.5412 47.4541 52.637 57.1971 59.7325 57.7872 52.5323 56.5723 61.6294
Actin 0.0073 0.004 0.0013 0.0073 0.0025 0.0033 0.0459 0.0067 0.0313 0.0112 0.0034 0.0018 0.0147 0.022 0.0027 0.0447 0.092 0.0202
Bud 0.0001 0 0 0 0.0001 0 0.0006 0.0003 0.0007 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0002 0
Bud Neck 0.0002 0.0001 0 0.0001 0.0001 0 0.0003 0 0.0002 0.0001 0 0 0.0005 0.0001 0.0014 0.0004 0.0015 0.0001
Bud Periphery 0.0001 0.0001 0 0.0001 0.0001 0 0.0012 0.0001 0.0009 0.0003 0 0 0.0001 0.0002 0.0001 0.0001 0.0006 0
Bud Site 0.0003 0.0001 0 0.0001 0.0001 0 0.0021 0.0018 0.001 0.0005 0 0 0.0002 0.0005 0.0003 0.0002 0.0001 0.0001
Cell Periphery 0.0004 0.0021 0.0002 0.0002 0.0002 0.0001 0.0004 0.0002 0.0001 0.0008 0 0.0001 0.0001 0.0002 0.0007 0.0001 0.0004 0.0001
Cytoplasm 0.0014 0 0 0 0.0003 0 0.0024 0.0001 0.0012 0.0008 0 0 0.0033 0 0.0015 0.0002 0.0001 0
Cytoplasmic Foci 0.0067 0.0003 0.0007 0.0004 0.0256 0.0001 0.0027 0.0003 0.0021 0.0132 0 0.0003 0.0178 0.0009 0.0079 0.0032 0.0009 0.0002
Eisosomes 0.005 0.0021 0.0021 0.012 0.001 0.0017 0.0022 0.0018 0.0012 0.001 0.007 0.0016 0.0007 0.0014 0.0008 0.001 0.0015 0.0051
Endoplasmic Reticulum 0.0013 0 0 0 0.0001 0 0.0014 0 0.001 0.0004 0 0.0001 0.0012 0.0001 0.0001 0.0002 0.0006 0
Endosome 0.0043 0.0005 0.0002 0.0001 0.0056 0.0002 0.0089 0.0006 0.0041 0.0067 0.0001 0.0021 0.006 0.0009 0.0013 0.0027 0.0014 0.0001
Golgi 0.0101 0.0052 0.0017 0.0047 0.0249 0.0016 0.0068 0.0073 0.0051 0.0065 0.0038 0.0179 0.0022 0.0068 0.0019 0.007 0.0129 0.0024
Lipid Particles 0.0263 0.0228 0.0322 0.0031 0.0196 0.014 0.0271 0.009 0.0062 0.099 0.0027 0.014 0.0089 0.0324 0.0127 0.054 0.0699 0.007
Mitochondria 0.9065 0.9432 0.9358 0.9604 0.8925 0.9656 0.8567 0.9558 0.9246 0.7119 0.9782 0.9479 0.9095 0.9098 0.9423 0.846 0.8066 0.9595
None 0.0009 0 0.0001 0.0001 0.0026 0 0.0009 0 0.0002 0.0023 0 0 0.0109 0 0.0004 0.001 0.0004 0
Nuclear Periphery 0.0029 0 0.0001 0 0.0002 0 0.0043 0 0.003 0.0056 0 0.0004 0.0052 0.0005 0.0001 0.0012 0.0016 0
Nucleolus 0.0013 0.001 0.0018 0.0064 0.0061 0.0001 0.0014 0.0002 0.0001 0.0076 0 0.0001 0.0005 0.0005 0.0006 0.0003 0.0002 0
Nucleus 0.0014 0.0001 0.0002 0.0005 0.0004 0 0.0093 0 0.0005 0.0015 0 0 0.0025 0.0001 0.0003 0.0001 0.0002 0
Peroxisomes 0.0034 0.0046 0.0034 0.0019 0.0048 0.0014 0.0074 0.0055 0.0109 0.0242 0.004 0.001 0.0069 0.0093 0.0041 0.0157 0.0026 0.0041
Punctate Nuclear 0.0017 0 0.0001 0 0.0011 0 0.0006 0 0.0003 0.0327 0 0 0.0058 0 0.0034 0.0062 0.0006 0
Vacuole 0.0014 0.0021 0.0011 0.0006 0.0014 0.0004 0.0098 0.0007 0.0009 0.0086 0 0.0003 0.0005 0.001 0.002 0.0002 0.0006 0.0001
Vacuole Periphery 0.0171 0.0117 0.0192 0.0021 0.0107 0.0114 0.0075 0.0093 0.0042 0.065 0.0006 0.0124 0.0025 0.0132 0.0155 0.0155 0.0054 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.4922 71.0191 91.7357 80.2301 74.3918 67.638 60.5043 78.9802 89.9498 81.8146
Translational Efficiency 2.7382 1.909 1.835 2.4841 2.1973 2.374 2.7574 2.3804 2.2602 2.3275

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2160 333 311 1277 1777 1686 1941 810 3937 2019 2252 2087

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2009.51 2531.95 2550.25 4300.90 2088.19 2377.60 3616.58 3842.05 2045.02 2403.06 3469.32 4122.81
Standard Deviation 592.60 645.97 728.11 1290.97 640.08 665.71 1045.80 1224.69 615.73 664.96 1072.95 1285.26
Intensity Change Log 2 — 0.333405 0.343795 1.097795 — 0.187253 0.792373 0.879623 — 0.260776 0.589715 0.990737

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.017382 0.022606 0.048878 0.014576 0.015493 0.019076 0.005157 0.016877 0.016529 0.019658 0.011194 0.015469
Bud Neck 0.001719 0.001958 0.009126 0.001756 0.001622 0.002063 0.001451 0.004044 0.001675 0.002045 0.002511 0.002644
Bud Site 0.009591 0.000918 0.005735 0.001703 0.001223 0.001894 0.002953 0.005942 0.005814 0.001733 0.003338 0.003348
Cell Periphery 0.001159 0.000085 0.000164 0.000430 0.000101 0.000096 0.000177 0.000610 0.000682 0.000094 0.000175 0.000500
Cytoplasm 0.003564 0.000682 0.001126 0.000287 0.000441 0.001010 0.000631 0.000950 0.002155 0.000956 0.000699 0.000544
Cytoplasmic Foci 0.036358 0.061368 0.068628 0.009220 0.036638 0.046764 0.012689 0.022380 0.036485 0.049173 0.020414 0.014328
Eisosomes 0.021126 0.017832 0.004548 0.007530 0.020255 0.009374 0.001806 0.003584 0.020733 0.010769 0.002185 0.005999
Endoplasmic Reticulum 0.004682 0.000158 0.000317 0.000248 0.000145 0.000151 0.000052 0.000616 0.002634 0.000152 0.000088 0.000391
Endosome 0.001514 0.000987 0.007267 0.000511 0.000659 0.001647 0.000466 0.002483 0.001128 0.001539 0.001405 0.001276
Golgi 0.023387 0.048657 0.105079 0.015819 0.027617 0.051493 0.006157 0.036574 0.025296 0.051025 0.019818 0.023874
Lipid Particles 0.013441 0.017037 0.031533 0.003669 0.017082 0.025324 0.003710 0.019126 0.015085 0.023958 0.007552 0.009668
Mitochondria 0.799507* 0.759415* 0.627764* 0.914760* 0.819301* 0.786673* 0.942669* 0.814730* 0.808441* 0.782177* 0.899180* 0.875937*
Mitotic Spindle 0.000284 0.003798 0.011674 0.000615 0.000330 0.001099 0.001631 0.002502 0.000305 0.001544 0.003018 0.001348
None 0.004474 0.004184 0.003313 0.003914 0.004558 0.003970 0.002422 0.006977 0.004512 0.004005 0.002545 0.005102
Nuclear Periphery 0.000204 0.000383 0.001820 0.000531 0.000179 0.000364 0.000366 0.002103 0.000193 0.000367 0.000567 0.001141
Nuclear Periphery Foci 0.000335 0.000186 0.000734 0.000292 0.000186 0.000227 0.000106 0.000599 0.000268 0.000220 0.000193 0.000411
Nucleolus 0.000869 0.001084 0.001238 0.000994 0.001978 0.001494 0.001151 0.001623 0.001370 0.001426 0.001163 0.001238
Nucleus 0.000766 0.000178 0.000167 0.000148 0.000274 0.000160 0.000190 0.000539 0.000544 0.000163 0.000187 0.000300
Peroxisomes 0.055234 0.052661 0.051929 0.007656 0.048091 0.039711 0.008223 0.007956 0.052010 0.041847 0.014258 0.007772
Vacuole 0.002743 0.002743 0.009346 0.003242 0.001901 0.003527 0.002779 0.013305 0.002363 0.003398 0.003686 0.007148
Vacuole Periphery 0.001660 0.003082 0.009614 0.012098 0.001924 0.003882 0.005215 0.036484 0.001779 0.003750 0.005823 0.021562

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.29 -4.23 1.26 1.92 4.56 -1.65 6.53 -0.55 0.74 -4.84 -1.71 3.38 0.61 1.98 -2.27
Bud Neck -0.51 -5.84 -0.08 0.40 5.75 -1.26 0.45 -4.24 -3.67 -4.83 -1.36 -2.65 -2.83 -1.81 -0.41
Bud Site 5.99 2.14 5.34 -1.50 3.30 -1.16 -2.07 -3.48 -3.04 -2.09 4.68 2.34 2.30 -2.55 -0.13
Cell Periphery 3.63 3.42 2.45 -3.93 -3.43 0.20 -1.62 -3.97 -4.06 -3.22 3.65 3.06 1.09 -5.90 -4.15
Cytoplasm 4.89 4.06 5.86 1.53 3.10 -2.23 -1.10 -1.73 -0.12 -0.90 3.26 3.98 4.59 1.31 0.55
Cytoplasmic Foci -3.81 -5.05 11.95 8.20 9.47 -3.53 10.39 4.12 6.81 -3.27 -5.05 8.01 11.65 14.00 3.06
Eisosomes 1.33 11.99 9.63 4.51 -2.96 7.82 14.61 12.58 7.98 -4.14 9.35 21.08 14.81 6.10 -7.61
Endoplasmic Reticulum 3.50 3.38 3.43 -1.64 1.29 -0.29 4.37 -4.06 -4.03 -4.89 3.50 3.59 3.15 -4.48 -5.70
Endosome 1.43 -4.68 3.28 2.06 5.67 -4.32 1.40 -4.36 -1.88 -4.94 -1.66 -1.07 -0.59 1.10 0.51
Golgi -4.70 -7.49 3.39 5.99 8.14 -7.30 12.06 -2.46 3.48 -9.19 -9.43 2.67 0.72 9.01 -1.68
Lipid Particles -1.14 -3.98 8.99 4.40 6.24 -3.46 8.09 -0.68 1.96 -5.83 -4.89 6.34 3.91 7.47 -1.60
Mitochondria 2.40 8.15 -14.69 -9.58 -13.80 3.51 -17.37 0.53 -2.26 12.70 3.39 -14.27 -10.19 -11.57 3.46
Mitotic Spindle -1.23 -3.65 -2.27 1.11 3.54 -1.47 -2.63 -1.94 -1.16 -0.66 -1.94 -4.47 -2.35 0.23 2.25
None 0.76 3.42 1.99 0.68 -1.75 2.40 10.38 -5.87 -7.36 -11.79 2.51 11.79 -2.48 -4.45 -11.76
Nuclear Periphery -2.14 -4.69 -3.49 -1.40 3.67 -5.92 -3.58 -6.69 -6.02 -5.95 -4.59 -5.30 -7.53 -6.15 -4.12
Nuclear Periphery Foci 0.93 -1.77 0.21 -0.58 1.89 -0.70 1.37 -3.97 -3.97 -5.53 0.51 0.83 -1.00 -1.67 -1.94
Nucleolus -0.70 -1.46 -0.35 0.24 0.56 0.88 1.37 0.60 -0.41 -1.14 -0.19 0.55 0.36 0.58 -0.22
Nucleus 2.03 2.35 2.04 -0.01 -0.14 1.28 0.63 -0.90 -1.26 -1.10 2.64 2.27 0.97 -1.15 -0.89
Peroxisomes 0.36 0.38 14.88 6.93 5.44 2.08 12.54 11.79 10.58 0.15 3.16 15.00 18.94 13.02 3.83
Vacuole 0.02 -3.52 -0.62 -0.48 3.24 -2.88 -1.92 -7.08 -5.86 -6.47 -2.04 -2.69 -6.29 -4.46 -4.24
Vacuole Periphery -1.30 -5.61 -6.75 -4.83 -1.19 -4.99 -5.26 -10.26 -9.64 -9.15 -5.40 -7.12 -12.11 -10.72 -9.16
Standard name
Human Ortholog
Description Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp14

Atp14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available