Standard name
Human Ortholog
Description Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.68 0.59 0.71 0.62 0.66 0.55 0.51 0.46 0.35 – 0.27 0.39 0.68 0.7 0.61 0.54 0.58 0.54 0.79 0.7 0.63 0.7 0.76 0.83
Bud 0.19 0.22 0.25 0.32 0.29 0.24 0.18 0.23 0.13 – 0.15 0.17 0.1 0.17 0.17 0.11 0.12 0.15 0.05 0.06 0.05 0.11 0.07 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0.08 0 0
Bud Site 0.1 0.06 0.14 0.06 0.07 0.08 0.06 0 0.06 – 0.05 0 0.1 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0.06 0.08 0.1 0 0 0.06 0 0.06 – 0 0.06 0.21 0.22 0.22 0.12 0.1 0.05 0 0 0 0 0 0
Cytoplasm 0.15 0.1 0.14 0.15 0.19 0.15 0.14 0.16 0.12 – 0.1 0.07 0.09 0.06 0.09 0.12 0.1 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.1 0 0 0.1 0.16 0.17 0.07 0.1 – 0.05 0.09 0.05 0 0.13 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0.06 0.07 0.45 0.44 0.4 0 0 0 0 0 0
Mitochondria 0.27 0.34 0.11 0.19 0.07 0.4 0.33 0.22 0.69 – 0.74 0.55 0.31 0.13 0.08 0.06 0.1 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.2 0.13 0.16 0.25 0.1 0.14 0.08 0.14 0.06 – 0.11 0.09 0.37 0.51 0.39 0.18 0.11 0.1 0 0 0.1 0.05 0.05 0
SpindlePole 0 0 0.09 0.06 0.05 0 0 0.05 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 130 148 146 45 107 96 163 144 76 – 66 109 100 192 173 107 113 81 177 211 155 112 208 212
Bud 37 56 51 23 47 42 58 70 29 – 38 48 14 47 49 22 24 22 11 19 12 17 18 9
Bud Neck 0 1 1 0 0 0 1 0 0 – 0 0 0 0 0 0 3 0 11 13 14 13 7 4
Bud Site 19 15 28 4 11 14 19 12 12 – 13 8 14 6 0 3 2 2 – – – – – –
Cell Periphery 14 15 16 7 6 5 19 11 14 – 10 17 31 62 61 23 20 7 0 1 1 0 3 3
Cytoplasm 28 26 28 11 31 26 46 51 26 – 25 21 13 16 25 24 19 23 2 2 4 0 1 1
Endoplasmic Reticulum 13 26 9 3 16 28 55 23 21 – 12 26 7 11 38 6 8 5 1 4 1 2 1 6
Endosome 1 3 4 1 3 0 6 8 2 – 2 5 3 1 5 5 3 5 4 14 8 2 5 4
Golgi 1 5 5 2 1 1 8 14 0 – 1 7 2 16 20 88 86 61 1 7 0 2 2 1
Mitochondria 51 85 22 14 12 70 106 67 149 – 183 154 45 37 23 12 20 17 4 11 10 0 3 3
Nucleus 0 0 3 1 2 0 6 8 3 – 7 3 0 0 0 0 4 0 0 0 1 0 0 0
Nuclear Periphery 1 2 0 0 0 0 1 0 2 – 3 0 0 1 1 0 0 1 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 1 0 4 1 – 3 1 0 0 0 0 2 1 0 0 0 0 0 0
Peroxisomes 39 32 32 18 17 25 26 43 12 – 26 25 54 141 109 35 22 15 3 6 23 8 13 6
SpindlePole 2 4 18 4 8 3 10 17 3 – 6 4 0 2 0 9 7 6 0 0 2 0 0 0
Vac/Vac Membrane 0 3 4 0 1 2 1 16 1 – 2 6 0 0 0 2 4 4 2 8 5 1 2 0
Unique Cell Count 191 252 205 72 163 175 318 311 217 247 282 146 276 282 197 196 151 225 303 246 162 273 257
Labelled Cell Count 336 421 367 133 262 313 525 488 351 397 434 283 532 504 336 337 250 225 303 246 162 273 257


Actin, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 7.7 7.3 6.9 7.7 6.5 6.8 7.2 6.1 – 5.5 6.5 8.6 9.4 9.7 14.3 14.7 14.4 7.5 8.2 9.3
Std Deviation (1e-4) 1.5 2.0 1.2 1.1 1.6 1.3 1.4 1.5 1.3 – 1.1 1.3 1.5 2.0 2.1 3.9 3.7 4.1 1.9 1.7 1.8
Intensity Change (Log2) – – – -0.09 0.07 -0.17 -0.1 -0.03 -0.27 – -0.4 -0.18 0.23 0.35 0.4 0.96 1.0 0.97 0.04 0.15 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -1.1 -3.3 -4.5 -5.6 -7.4 – -9.4 -7.1 -0.5 -0.4 -2.3 -3.5 -2.8 -3.4
Bud 1.2 0.9 -0.2 -1.8 -0.6 -3.0 – -2.5 -2.1 -3.6 -2.1 -2.0 -3.6 -3.2 -2.4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site -1.9 -2.1 -1.8 -3.0 -4.1 -2.8 – -3.1 -4.5 -1.2 -4.9 -6.4 -4.6 -4.8 -4.1
Cell Periphery 0.5 -1.7 -2.1 -0.8 -2.1 -0.5 – -1.7 -0.8 3.6 4.3 4.1 1.3 0.8 -1.2
Cytoplasm 0.3 1.4 0.3 0.3 0.8 -0.5 – -1.2 -2.2 -1.4 -3.0 -1.7 -0.4 -1.2 0.4
Endoplasmic Reticulum 0 2.1 3.8 4.4 1.4 2.1 – 0.2 2.0 0.2 -0.2 3.4 -0.7 -0.2 -0.5
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.1 1.2 0 – 0 0 0 1.8 2.3 10.0 9.9 9.1
Mitochondria 1.9 -1.1 6.6 5.9 3.2 12.1 – 13.5 10.0 4.7 0.9 -1.0 -1.7 -0.2 0.2
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1.8 -1.5 -0.4 -2.6 -0.6 -3.4 – -1.6 -2.3 4.6 8.0 5.5 0.6 -1.3 -1.6
SpindlePole -0.9 -1.4 -3.0 -2.8 -1.5 -3.5 – -3.0 -3.9 -3.7 -4.4 -5.1 -1.7 -2.2 -1.8
Vacuole 0 0 0 0 1.8 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7834 5.4234 4.7396 3.9054 4.157 5.4088 4.8852 4.5906 4.2876 3.861 4.1827 4.7397 4.6117 4.6434 4.1215 4.3537 3.9918 4.1095
Actin 0.7735 0.6917 0.7755 0.8071 0.7366 0.8106 0.8698 0.7295 0.8261 0.799 0.8791 0.8881 0.8238 0.7056 0.8372 0.9402 0.7605 0.8281
Bud 0.001 0.0037 0.0196 0.0099 0.0174 0.0011 0.0017 0.0041 0.0198 0.0262 0.0111 0.0014 0.0017 0.0074 0.0321 0.0139 0.0062 0.0014
Bud Neck 0.0222 0.018 0.0111 0.0008 0.0018 0.1518 0.007 0.0051 0.0054 0.0017 0.0011 0.0784 0.0209 0.0075 0.0053 0.0058 0.0209 0.1192
Bud Periphery 0.0022 0.0036 0.041 0.0941 0.063 0.0079 0.0044 0.004 0.0314 0.1057 0.0257 0.0037 0.0025 0.0082 0.059 0.0282 0.0286 0.0033
Bud Site 0.0407 0.2227 0.0412 0.002 0.0072 0.0045 0.0348 0.2121 0.054 0.0053 0.0089 0.0031 0.0423 0.2151 0.0413 0.0032 0.0346 0.0031
Cell Periphery 0.0028 0.0015 0.0019 0.001 0.0008 0.0017 0.0012 0.0012 0.0008 0.0038 0.0007 0.0007 0.0018 0.0019 0.0011 0.0002 0.0015 0.0005
Cytoplasm 0.0039 0.0274 0.0127 0.0005 0.0026 0.0018 0.0054 0.0195 0.0108 0.0124 0.0018 0.001 0.0051 0.0198 0.0039 0.0009 0.044 0.002
Cytoplasmic Foci 0.0239 0.0156 0.0176 0.0046 0.0101 0.0024 0.0102 0.0039 0.0055 0.0179 0.009 0.0029 0.0237 0.0098 0.0046 0.0009 0.0159 0.0041
Eisosomes 0.0057 0.0017 0.0014 0.0009 0.0023 0.0027 0.0098 0.0008 0.001 0.001 0.0018 0.0018 0.0065 0.0008 0.0006 0.0006 0.0007 0.0011
Endoplasmic Reticulum 0.0008 0.0002 0.0009 0.0001 0.0004 0.0001 0.0014 0.0002 0.0007 0.0005 0.0009 0.0008 0.0009 0.0002 0.0002 0.0001 0.0005 0.0007
Endosome 0.0091 0.0004 0.0076 0.024 0.002 0.0002 0.0095 0.0022 0.0052 0.0002 0.0173 0.0024 0.0115 0.0011 0.0024 0.0009 0.0195 0.0067
Golgi 0.0091 0.0015 0.0048 0.0284 0.0096 0.0017 0.0124 0.0087 0.0065 0.0009 0.003 0.0049 0.0139 0.0015 0.0038 0.002 0.0136 0.0074
Lipid Particles 0.0221 0.0003 0.0077 0.001 0.0055 0.0002 0.0057 0.0002 0.0046 0.0023 0.002 0.0004 0.0085 0.0043 0.0037 0 0.0218 0.004
Mitochondria 0.0081 0.0003 0.0047 0.0079 0.0713 0.0002 0.0026 0.0032 0.0046 0.0009 0.0009 0.0021 0.0072 0.0021 0.0009 0.0021 0.0255 0.0029
None 0.0227 0.0026 0.019 0.0016 0.0297 0.0056 0.0011 0.0002 0.0051 0.0164 0.0005 0.0018 0.0008 0.0063 0.0002 0.0001 0.0003 0.0002
Nuclear Periphery 0.0036 0 0.0004 0.0001 0.0002 0.0001 0.005 0.0001 0.002 0.0002 0.0016 0.0022 0.0007 0.0001 0.0001 0 0.0004 0.0018
Nucleolus 0.0018 0.0001 0.0005 0 0.0001 0.0012 0.0003 0 0.0001 0.0009 0.0003 0.0001 0.0001 0 0 0 0.0001 0.0001
Nucleus 0.0045 0.0001 0.0004 0 0.0001 0.0008 0.0016 0.0001 0.0002 0.0007 0.0016 0.0004 0.0002 0.0001 0.0001 0 0.0002 0.0019
Peroxisomes 0.0324 0.0085 0.0267 0.0153 0.0384 0.0038 0.0119 0.004 0.0147 0.0007 0.0253 0.003 0.0246 0.0071 0.0032 0.0007 0.0027 0.0105
Punctate Nuclear 0.0085 0.0002 0.0035 0.0001 0.0004 0.0014 0.003 0.0001 0.0004 0.0013 0.0051 0.0006 0.0027 0.0002 0.0002 0 0.0004 0.0005
Vacuole 0.0008 0.0001 0.0014 0.0003 0.0003 0.0001 0.0006 0.0008 0.0005 0.0018 0.002 0.0001 0.0005 0.0005 0.0002 0.0002 0.0011 0.0003
Vacuole Periphery 0.0007 0 0.0006 0.0004 0.0002 0 0.0005 0.0001 0.0005 0.0002 0.0004 0.0001 0.0003 0.0001 0 0.0001 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7139 14.872 18.7905 28.1558 11.0765 18.4248 22.0476 23.4524 25.1283 24.8132
Translational Efficiency 2.7712 2.0023 1.9157 1.4595 3.1153 2.8346 1.4369 1.6717 1.8388 1.5782

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)
Localization
Cell Percentages actin (98%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vrp1

Vrp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vrp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available