Standard name
Human Ortholog
Description 1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 – 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.08 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.99 1.0 0.98 0.97 0.94 0.93 – 0.86 0.82 0.66 0.81 – 0.98 0.99 0.95 0.95 0.93 0.72 0.87 0.73 0.85 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.1 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 – 0.22 0.16 0.46 0.24 – 0 0 0 0 0 0.15 0.05 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 2 – 0 0 0 0 – 0 0 0 1 0 3 0 1 0 0 1
Bud 0 0 0 0 0 2 2 – 0 2 10 3 – 0 0 0 0 0 2 0 0 0 0 1
Bud Neck 1 0 0 7 21 7 18 – 10 17 8 7 – 0 1 0 3 1 0 0 1 0 0 0
Bud Site 0 0 1 0 0 0 0 – 1 0 0 1 – 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 3 2 2 1 – 0 0 1 0 – 1 0 8 3 7 0 0 1 0 0 0
Cytoplasm 182 149 44 217 423 232 434 – 235 272 167 206 – 149 212 92 145 143 141 151 60 24 57 80
Endoplasmic Reticulum 2 1 0 2 6 0 3 – 0 0 2 1 – 0 0 10 5 10 2 1 0 0 0 0
Endosome 0 0 0 1 0 0 1 – 0 0 0 1 – 0 0 1 1 3 2 0 0 0 0 3
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0
Mitochondria 8 1 0 0 1 11 33 – 60 53 116 60 – 3 0 2 3 4 28 8 11 1 0 2
Nucleus 0 1 0 0 2 2 5 – 2 7 2 3 – 0 0 1 0 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 1 2 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 3 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 5 2 10 – 5 8 8 7 – 0 1 0 2 1 2 1 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 6 2 – 2 4 0 1 – 1 0 1 1 7 5 2 2 0 0 2
Unique Cell Count 190 151 44 222 435 247 469 274 331 252 254 152 214 97 152 153 196 174 83 29 65 96
Labelled Cell Count 194 152 45 233 462 264 512 316 365 314 291 154 215 115 167 176 196 174 83 29 65 96


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 4.3 4.7 4.9 4.1 4.2 – 3.8 3.8 3.4 3.5 – 4.9 4.7 5.1 5.0 5.2 3.7 3.8 5.2
Std Deviation (1e-4) 0.4 0.8 0.8 1.3 1.5 1.5 1.4 – 1.8 1.3 1.6 1.1 – 1.5 1.3 0.8 0.6 1.3 0.3 0.4 1.8
Intensity Change (Log2) – – – 0.12 0.18 -0.08 -0.05 – -0.17 -0.18 -0.34 -0.3 – 0.18 0.13 0.25 0.21 0.28 -0.23 -0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1222 -1.1216 -0.8728 -1.0693 -1.0097 -1.1731 -0.4206 -0.8071 -0.6158 -0.766 -0.8687 -0.7776 -1.7133 -1.8905 -1.781 -1.8619 -1.2208 -1.7687
Actin 0.0206 0.0001 0.0237 0.005 0.0065 0.0083 0.0377 0.0005 0.0161 0.0075 0.0224 0.0002 0.0319 0.0027 0.0242 0.0365 0.0041 0.004
Bud 0.0003 0 0.0023 0.0001 0.0001 0.0056 0.0037 0.0014 0.0013 0.0085 0.0001 0.0002 0.0015 0.002 0.0008 0.0001 0.009 0.0002
Bud Neck 0.0011 0 0.0046 0.0003 0.0002 0.0013 0.0023 0.0001 0.0014 0.0003 0.0003 0.0001 0.0013 0.0002 0.0003 0.0001 0.0018 0.0027
Bud Periphery 0.0005 0 0.0028 0.0001 0.0001 0.002 0.0057 0.0006 0.0007 0.0058 0.0002 0.0002 0.0089 0.004 0.0011 0.0001 0.0104 0.0003
Bud Site 0.0026 0.0001 0.0106 0.0006 0.0002 0.0118 0.0196 0.0046 0.008 0.0018 0.0006 0 0.0029 0.0077 0.0014 0.0002 0.0495 0.0028
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0.0002 0.0017 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0003 0.0001 0 0.0004 0.0001
Cytoplasm 0.0101 0.0159 0.0088 0.0221 0.0123 0.0328 0.0165 0.0214 0.0147 0.0127 0.0435 0.0311 0.0216 0.0374 0.0303 0.0149 0.0215 0.0509
Cytoplasmic Foci 0.0291 0.0014 0.0176 0.0353 0.009 0.0142 0.0388 0.0042 0.0091 0.0193 0.0113 0.0063 0.0387 0.0047 0.0191 0.0064 0.0212 0.0057
Eisosomes 0.0018 0.0002 0.0006 0.0002 0.0005 0.0002 0.0009 0.0001 0.0006 0.0002 0.0001 0.0001 0.0006 0.0003 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0017 0.0003 0.0005 0.0006 0.0007 0.0003 0.0055 0.0002 0.0006 0.0006 0.0023 0.0002 0.0014 0.0006 0.0005 0.0002 0.0003 0.0003
Endosome 0.0143 0.0001 0.0084 0.0056 0.0087 0.001 0.0262 0.0005 0.0062 0.0126 0.0103 0.0002 0.0137 0.0017 0.0049 0.0004 0.0069 0.004
Golgi 0.0077 0 0.0065 0.002 0.0052 0.0012 0.0075 0.0005 0.0034 0.0105 0.0098 0 0.008 0.0006 0.0055 0.0016 0.0082 0.0044
Lipid Particles 0.0162 0.0003 0.0089 0.0058 0.0377 0.0027 0.0117 0.0004 0.0055 0.0098 0.0077 0.0002 0.013 0.0005 0.0112 0.001 0.0258 0.001
Mitochondria 0.0031 0 0.0036 0.0004 0.0025 0.0003 0.0197 0.0106 0.012 0.0072 0.0035 0.0003 0.0028 0.0019 0.0023 0.0006 0.0051 0.002
None 0.8491 0.981 0.8852 0.9037 0.9042 0.9116 0.7388 0.9465 0.9022 0.8706 0.8786 0.958 0.7975 0.9316 0.8781 0.9191 0.7954 0.9177
Nuclear Periphery 0.0031 0.0001 0.0015 0.0006 0.0011 0.0003 0.0058 0.0002 0.001 0.0021 0.0012 0.0001 0.003 0.0003 0.001 0.0001 0.0003 0.0001
Nucleolus 0.0018 0.0001 0.0009 0.0003 0.0003 0.0005 0.0031 0.0006 0.0043 0.0015 0.0001 0.0002 0.0018 0.0002 0.0004 0 0.0047 0.0001
Nucleus 0.0017 0.0001 0.0006 0.0007 0.0002 0.0007 0.0035 0.0015 0.0026 0.0107 0.0005 0.0003 0.0043 0.0004 0.0004 0.0001 0.0019 0.0003
Peroxisomes 0.0273 0 0.0092 0.0062 0.0076 0.0016 0.0262 0.0005 0.0052 0.0093 0.0042 0.0001 0.0278 0.0003 0.0142 0.0183 0.0286 0.002
Punctate Nuclear 0.0062 0.0001 0.0023 0.009 0.0023 0.0028 0.014 0.0053 0.0034 0.0075 0.0024 0.0019 0.0173 0.0005 0.0034 0.0002 0.002 0.0007
Vacuole 0.0008 0.0001 0.0008 0.0012 0.0003 0.0005 0.0093 0.0002 0.0009 0.0008 0.0003 0.0002 0.0013 0.0017 0.0003 0.0001 0.0025 0.0004
Vacuole Periphery 0.0006 0 0.0005 0.0002 0.0003 0.0001 0.0018 0.0001 0.0004 0.0005 0.0002 0.0001 0.0003 0.0004 0.0001 0 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9614 5.9135 0.8776 1.9909 3.0019 2.6446 2.5411 1.6142 1.0533 4.1852
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1405 780 475 358 1637 1647 132 835 3042 2427 607 1193

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 508.44 627.46 854.58 839.73 667.64 668.03 904.48 770.02 594.11 654.99 865.43 790.94
Standard Deviation 77.13 87.63 104.47 126.44 73.25 81.91 80.33 94.10 109.24 85.91 101.82 109.62
Intensity Change Log 2 — 0.303446 0.749138 0.723848 — 0.000843 0.438018 0.205826 — 0.139512 0.580818 0.452850

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000091 0.000204 0.001256 0.001575 0.000163 0.000311 0.001318 0.000529 0.000130 0.000277 0.001269 0.000842
Bud Neck 0.020014 0.031419 0.005796 0.007193 0.012447 0.050866 0.001425 0.008117 0.015942 0.044616 0.004845 0.007840
Bud Site 0.004623 0.009469 0.006739 0.050921 0.003131 0.016208 0.001947 0.027803 0.003820 0.014042 0.005697 0.034740
Cell Periphery 0.000624 0.000145 0.000198 0.000215 0.000141 0.000158 0.000317 0.000357 0.000364 0.000154 0.000224 0.000315
Cytoplasm 0.443848* 0.391213* 0.194324* 0.351997* 0.460637* 0.403511* 0.357936* 0.445372* 0.452882* 0.399558* 0.229903* 0.417351*
Cytoplasmic Foci 0.149210 0.252087* 0.007319 0.025480 0.237454* 0.224114* 0.043213 0.037893 0.196697* 0.233104* 0.015125 0.034168
Eisosomes 0.000433 0.000141 0.000059 0.000071 0.000162 0.000141 0.000145 0.000080 0.000288 0.000141 0.000078 0.000078
Endoplasmic Reticulum 0.001357 0.000742 0.009104 0.003424 0.000708 0.000537 0.005203 0.001575 0.001007 0.000603 0.008255 0.002130
Endosome 0.002874 0.026558 0.003250 0.007963 0.010244 0.023819 0.011192 0.007601 0.006840 0.024699 0.004977 0.007709
Golgi 0.001826 0.003226 0.000780 0.011160 0.002894 0.005603 0.004106 0.002892 0.002401 0.004839 0.001503 0.005373
Lipid Particles 0.015915 0.011349 0.001895 0.001981 0.011234 0.007397 0.007832 0.002244 0.013396 0.008667 0.003186 0.002165
Mitochondria 0.008107 0.013578 0.000591 0.003913 0.005907 0.017401 0.000400 0.007869 0.006923 0.016172 0.000549 0.006682
Mitotic Spindle 0.000094 0.001766 0.001451 0.069905 0.002223 0.008675 0.000217 0.028047 0.001240 0.006455 0.001183 0.040608
None 0.018751 0.007727 0.003984 0.006246 0.003241 0.003704 0.002599 0.005093 0.010405 0.004997 0.003683 0.005439
Nuclear Periphery 0.000621 0.000651 0.001902 0.004122 0.000553 0.000432 0.001987 0.001383 0.000584 0.000502 0.001920 0.002205
Nuclear Periphery Foci 0.000199 0.000559 0.002063 0.005708 0.000308 0.000209 0.002966 0.001485 0.000257 0.000322 0.002260 0.002752
Nucleolus 0.002552 0.002810 0.000687 0.000501 0.001201 0.002366 0.000666 0.000796 0.001825 0.002509 0.000682 0.000707
Nucleus 0.310165* 0.166263* 0.702509* 0.279080* 0.201774* 0.140213* 0.404848* 0.303237* 0.251836* 0.148585* 0.637778* 0.295988*
Peroxisomes 0.004808 0.016224 0.001269 0.005235 0.006828 0.016430 0.001229 0.002388 0.005895 0.016364 0.001261 0.003242
Vacuole 0.013139 0.061081 0.054576 0.161542 0.037445 0.075556 0.150148 0.111675 0.026219 0.070904 0.075360 0.126639
Vacuole Periphery 0.000747 0.002789 0.000250 0.001769 0.001308 0.002348 0.000305 0.003566 0.001049 0.002490 0.000262 0.003027

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.79 -2.77 -4.62 -3.99 0.34 -1.66 -1.29 -2.38 -1.36 0.75 -2.82 -2.97 -4.89 -3.51 1.42
Bud Neck -3.02 6.65 6.98 7.96 -0.24 -12.94 11.90 4.55 14.91 -6.01 -11.97 8.66 7.93 16.81 -1.68
Bud Site -3.26 -1.00 -4.85 -3.91 -4.25 -8.59 1.37 -4.95 -0.62 -5.28 -8.93 -1.22 -6.78 -2.84 -5.34
Cell Periphery 8.55 6.87 8.22 -1.05 2.42 -0.79 -6.33 -1.26 -1.02 1.17 7.39 3.95 2.86 -1.13 0.64
Cytoplasm 4.72 24.59 12.97 8.03 -5.03 6.05 7.16 9.33 4.11 -2.32 7.47 25.77 14.23 7.55 -10.46
Cytoplasmic Foci -11.49 32.98 28.60 28.88 -4.69 1.88 28.04 37.33 34.56 2.23 -6.28 48.03 45.25 44.65 -5.64
Eisosomes 8.34 10.33 10.13 6.24 -1.22 2.16 -0.22 8.62 6.85 5.47 8.53 10.98 12.02 8.99 1.66
Endoplasmic Reticulum 1.97 -13.07 -6.90 -10.10 9.18 1.52 -7.73 -10.57 -11.41 5.02 2.62 -15.11 -10.48 -15.09 11.80
Endosome -10.21 -1.53 -0.88 9.01 -0.13 -8.65 -0.14 5.08 11.42 3.13 -14.01 2.79 2.85 14.50 0.48
Golgi -1.69 2.63 -0.37 0.15 -0.90 -3.07 0.24 2.24 4.77 0.44 -3.82 2.36 0.75 3.10 -0.85
Lipid Particles 2.97 14.98 15.46 6.67 1.12 4.10 2.76 11.13 9.27 4.40 5.94 14.95 18.53 11.07 3.87
Mitochondria -2.04 8.49 6.02 4.94 -1.25 -6.50 5.84 1.12 6.27 -2.27 -6.44 9.69 2.95 7.72 -2.32
Mitotic Spindle -3.05 -3.45 -4.91 -4.70 -4.71 -3.86 2.76 -4.89 -2.68 -5.64 -4.67 -0.17 -7.05 -5.23 -7.03
None 5.01 8.01 6.56 1.67 -1.69 -0.49 2.16 -0.49 -0.11 -1.72 5.00 7.74 5.25 0.76 -1.39
Nuclear Periphery -0.26 -15.09 -3.82 -3.75 -1.31 1.99 -11.08 -14.04 -15.63 0.38 1.57 -18.11 -8.66 -8.93 -0.78
Nuclear Periphery Foci -3.66 -8.79 -2.57 -2.16 0.14 1.78 -7.09 -4.54 -5.15 4.11 -1.29 -10.54 -4.47 -4.26 2.86
Nucleolus -0.54 3.38 3.79 6.93 2.44 -3.72 4.33 2.65 5.47 -0.72 -2.11 4.43 4.31 7.99 0.41
Nucleus 15.05 -28.31 1.85 -7.26 22.05 9.50 -7.94 -12.21 -18.37 2.38 18.74 -30.38 -7.20 -19.21 21.97
Peroxisomes -6.46 6.33 3.95 8.00 -0.75 -7.72 7.38 6.81 14.08 -0.87 -10.63 8.99 8.20 16.02 -1.05
Vacuole -13.69 -11.70 -20.03 -16.51 -14.21 -11.77 -9.50 -20.18 -15.14 0.59 -18.47 -13.13 -28.24 -21.70 -12.42
Vacuole Periphery -5.28 3.86 0.53 4.72 -1.16 -2.89 3.49 -0.58 1.01 -2.37 -5.78 4.67 -0.68 2.37 -2.59
Standard name
Human Ortholog
Description 1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p
Localization
Cell Percentages cytoplasm (26%), nucleus (9%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gas2

Gas2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gas2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available