Standard name
Human Ortholog
Description Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.92 0.9 0.9 – 0.75 0.62 0.64 0.65 0.43 – 0.46 0.96 0.97 0.99 0.94 0.94 0.92 0.93 0.87 0.71 0.8 0.79 0.79
Endoplasmic Reticulum 0 0 0 0 – 0.12 0.05 0.05 0 0 – 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0.06
Golgi 0 0 0 0 – 0 0 0.05 0 0.05 – 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0.06 0.05 0.05 0.24 0.36 – 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0.1 – 0.12 0.23 0.2 0.12 0.21 – 0.07 0 0 0 0.06 0.11 0.1 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 6 2 0 – 0 2 4 1 1 – 1 0 1 0 1 0 0 1 6 0 1 2 2
Bud 1 6 0 0 – 0 0 0 0 0 – 0 0 0 0 2 0 0 0 5 1 1 2 3
Bud Neck 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 2 2 4
Bud Site 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 26 3 0 – 0 2 2 0 0 – 0 2 4 5 26 13 29 0 1 0 0 1 0
Cytoplasm 450 756 43 44 – 12 35 41 22 35 – 19 171 185 241 700 428 571 454 740 47 196 231 234
Endoplasmic Reticulum 3 17 2 0 – 2 3 3 1 2 – 2 7 3 2 32 19 17 0 6 3 5 7 4
Endosome 2 3 1 0 – 0 0 0 0 1 – 0 0 0 0 1 1 1 7 20 4 10 11 17
Golgi 2 4 1 0 – 0 2 3 0 4 – 3 0 1 0 0 1 3 1 12 1 2 6 1
Mitochondria 1 6 0 0 – 1 3 3 8 29 – 18 1 0 0 13 6 2 1 12 1 2 5 3
Nucleus 4 3 0 0 – 0 1 1 0 1 – 0 1 1 0 6 6 11 4 7 0 1 2 1
Nuclear Periphery 0 1 0 0 – 0 0 1 0 1 – 0 0 0 0 0 0 0 1 3 1 1 2 3
Nucleolus 0 1 0 0 – 0 0 0 0 2 – 0 0 0 0 0 0 0 0 2 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 3 4 2
Vac/Vac Membrane 12 32 4 5 – 2 13 13 4 17 – 3 1 1 1 46 49 64 8 25 1 11 7 10
Unique Cell Count 469 820 48 49 16 56 64 34 81 41 179 191 243 745 455 620 487 854 66 245 294 296
Labelled Cell Count 480 861 56 49 17 61 71 36 93 46 183 196 249 827 523 698 487 854 66 245 294 296


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.0 10.9 12.1 11.7 – 11.5 12.4 12.5 10.5 10.2 – 9.7 12.2 11.9 11.7 11.1 13.5 14.4 12.3 13.2 13.4
Std Deviation (1e-4) 2.0 2.0 2.3 2.3 – 2.1 2.4 2.7 2.3 2.4 – 2.3 2.2 1.9 1.8 2.1 2.5 3.1 2.2 2.2 2.5
Intensity Change (Log2) – – – -0.06 – -0.08 0.03 0.04 -0.21 -0.25 – -0.33 0.01 -0.03 -0.06 -0.13 0.15 0.24 0.02 0.12 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 – -1.5 -3.2 -3.1 -2.7 -5.2 – -4.4 1.6 2.2 4.0 1.2 1.2 0.6
Endoplasmic Reticulum 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 4.7 – 5.1 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0
Vacuole 0 – 0 2.0 1.7 0 1.9 – 0 0 0 0 0 0.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.1506 9.8479 8.9938 8.3396 7.3818 8.8502 11.0548 13.2818 12.6288 12.2056 12.8035 12.8116 11.0375 12.0007 11.2846 10.2697 11.3636 10.9133
Actin 0.0596 0.0104 0.0296 0.0343 0.0477 0.0251 0.0414 0.0084 0.0815 0.0855 0.0103 0.011 0.0115 0.0022 0.0069 0.0063 0.0033 0.0024
Bud 0.0021 0.0045 0.0043 0.0194 0.0033 0.0029 0.0087 0.0073 0.0142 0.0092 0.0029 0.0041 0.0012 0.0013 0.0032 0.0054 0.0167 0.0015
Bud Neck 0.0032 0.0009 0.0033 0.0022 0.0024 0.0041 0.0021 0.0016 0.0015 0.0012 0.0031 0.0041 0.0022 0.0009 0.0036 0.0014 0.0012 0.0026
Bud Periphery 0.0014 0.0035 0.0031 0.0193 0.0032 0.0022 0.0079 0.0054 0.0256 0.023 0.001 0.0021 0.0009 0.0004 0.0018 0.0049 0.0141 0.0006
Bud Site 0.015 0.008 0.0078 0.0052 0.0051 0.0029 0.005 0.0257 0.0223 0.0074 0.0034 0.0021 0.0028 0.003 0.0058 0.0024 0.004 0.0007
Cell Periphery 0.0005 0.0005 0.0008 0.0007 0.0003 0.0003 0.0006 0.0006 0.0009 0.0045 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.5145 0.8327 0.7327 0.6109 0.6541 0.6592 0.4496 0.7685 0.6619 0.5769 0.6317 0.6491 0.7588 0.8846 0.8158 0.7741 0.7493 0.7749
Cytoplasmic Foci 0.0922 0.0551 0.067 0.0768 0.0779 0.0772 0.1374 0.0493 0.0489 0.0784 0.1254 0.0936 0.0546 0.0341 0.0476 0.0562 0.0457 0.0656
Eisosomes 0.0003 0 0.0001 0.0001 0.0004 0.0001 0.0003 0 0.0003 0.0004 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0417 0.0043 0.0164 0.0065 0.0052 0.0217 0.0246 0.0052 0.0095 0.0042 0.0054 0.0188 0.0133 0.0053 0.0069 0.008 0.0076 0.0103
Endosome 0.1435 0.0343 0.0554 0.0905 0.0641 0.0975 0.1138 0.0595 0.0447 0.0185 0.0727 0.1384 0.0675 0.0362 0.0577 0.0835 0.086 0.0826
Golgi 0.0392 0.0173 0.0299 0.0449 0.0385 0.0442 0.0726 0.0169 0.036 0.0341 0.0488 0.0368 0.0135 0.0074 0.0134 0.0201 0.0198 0.0152
Lipid Particles 0.0064 0.0005 0.0005 0.0002 0.019 0.0014 0.0101 0.0007 0.0006 0.0005 0.0334 0.0019 0.0025 0.0002 0.0003 0.0007 0.0133 0.0003
Mitochondria 0.0184 0.0078 0.0323 0.0669 0.0323 0.0354 0.0267 0.0047 0.0399 0.1013 0.0213 0.0085 0.0187 0.0041 0.0161 0.0117 0.0139 0.0161
None 0.007 0.0066 0.0014 0.0018 0.0038 0.0031 0.0077 0.0016 0.0014 0.0012 0.0038 0.0039 0.0124 0.0023 0.0034 0.0034 0.0024 0.0036
Nuclear Periphery 0.0173 0.0014 0.0025 0.0012 0.0075 0.0033 0.0149 0.0071 0.0024 0.001 0.0053 0.0058 0.0076 0.0025 0.0018 0.0015 0.0049 0.0027
Nucleolus 0.0003 0.0001 0 0.0001 0.0006 0.0001 0.0007 0.0002 0.0001 0.0001 0.0004 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0076 0.0017 0.0018 0.0021 0.0033 0.0042 0.0425 0.0193 0.0019 0.0016 0.002 0.0059 0.0132 0.0057 0.0049 0.0034 0.0047 0.007
Peroxisomes 0.0104 0.0061 0.007 0.0068 0.0205 0.0064 0.0098 0.0026 0.003 0.0459 0.0175 0.0054 0.0037 0.0021 0.0026 0.0081 0.0026 0.0056
Punctate Nuclear 0.0033 0.0006 0.0005 0.0006 0.0074 0.0007 0.0157 0.002 0.0004 0.0004 0.0007 0.0012 0.0037 0.0023 0.0013 0.0005 0.0015 0.0007
Vacuole 0.0122 0.0027 0.0025 0.0058 0.0019 0.0053 0.0049 0.0109 0.0019 0.0032 0.0045 0.0045 0.0083 0.0038 0.0049 0.0056 0.0054 0.0055
Vacuole Periphery 0.0039 0.0007 0.0012 0.0037 0.0016 0.0026 0.0029 0.0024 0.001 0.0014 0.0059 0.0025 0.0028 0.0012 0.0015 0.0026 0.0032 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 107.6953 54.048 69.3499 88.0411 71.0407 89.3817 86.2412 91.4407 87.6411 93.2171
Translational Efficiency 0.9815 1.8688 1.3432 0.8518 1.2297 1.3471 1.1658 1.1151 1.2473 1.1847

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
474 820 2855 1872 1875 2003 1743 1436 2349 2823 4598 3308

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 850.28 1074.02 1181.22 1237.49 984.23 1194.19 1199.57 1244.17 957.20 1159.28 1188.18 1240.39
Standard Deviation 116.29 199.45 173.55 195.74 184.79 170.57 179.85 210.36 181.31 187.55 176.19 202.24
Intensity Change Log 2 — 0.337011 0.474268 0.541407 — 0.278965 0.285450 0.338116 — 0.306159 0.376046 0.435911

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002200 0.002606 0.000201 0.022463 0.000635 0.001052 0.000266 0.009660 0.000951 0.001503 0.000226 0.016905
Bud Neck 0.007005 0.005516 0.006503 0.012431 0.004964 0.014205 0.002961 0.009211 0.005376 0.011681 0.005160 0.011033
Bud Site 0.137409* 0.110333* 0.012472 0.170030* 0.042085 0.064202 0.007129 0.173167* 0.061320 0.077602 0.010447 0.171391*
Cell Periphery 0.000393 0.000133 0.000073 0.000225 0.000092 0.000311 0.000088 0.000257 0.000153 0.000259 0.000079 0.000239
Cytoplasm 0.154118* 0.490893* 0.721124* 0.395481* 0.608782* 0.430333* 0.686839* 0.468424* 0.517037* 0.447924* 0.708128* 0.427146*
Cytoplasmic Foci 0.330558* 0.212752* 0.018945 0.095503 0.176126 0.242246* 0.018824 0.092128 0.207289* 0.233678* 0.018900 0.094038
Eisosomes 0.000065 0.000024 0.000012 0.000049 0.000018 0.000036 0.000015 0.000029 0.000028 0.000033 0.000013 0.000041
Endoplasmic Reticulum 0.004201 0.001223 0.000772 0.000812 0.002994 0.001328 0.001614 0.000538 0.003237 0.001297 0.001091 0.000693
Endosome 0.054772 0.030410 0.002628 0.029602 0.016680 0.041769 0.003427 0.021210 0.024366 0.038470 0.002931 0.025959
Golgi 0.113034* 0.036267 0.000420 0.033552 0.018936 0.029306 0.000320 0.033543 0.037924 0.031328 0.000382 0.033548
Lipid Particles 0.003117 0.001170 0.000850 0.001754 0.001754 0.001397 0.001028 0.001599 0.002029 0.001331 0.000918 0.001687
Mitochondria 0.068809 0.005775 0.000784 0.016960 0.009407 0.014539 0.000303 0.007151 0.021394 0.011993 0.000602 0.012702
Mitotic Spindle 0.019711 0.014581 0.008039 0.058645 0.010949 0.016299 0.007849 0.063299 0.012717 0.015800 0.007967 0.060665
None 0.000655 0.000752 0.003182 0.000595 0.001346 0.001016 0.005165 0.000398 0.001206 0.000940 0.003934 0.000510
Nuclear Periphery 0.000152 0.000078 0.000436 0.000477 0.000245 0.000215 0.000739 0.000179 0.000226 0.000175 0.000551 0.000347
Nuclear Periphery Foci 0.001554 0.001822 0.000712 0.001163 0.001504 0.000653 0.002767 0.001010 0.001514 0.000993 0.001491 0.001097
Nucleolus 0.000447 0.000431 0.000768 0.000357 0.001453 0.000783 0.000652 0.000535 0.001250 0.000681 0.000724 0.000434
Nucleus 0.003863 0.011193 0.138240* 0.008699 0.026576 0.014941 0.152143* 0.009833 0.021993 0.013852 0.143510* 0.009191
Peroxisomes 0.010746 0.008975 0.000734 0.036143 0.002569 0.009959 0.000438 0.025792 0.004219 0.009673 0.000622 0.031650
Vacuole 0.035866 0.054561 0.082236 0.103302 0.059232 0.102544 0.106752 0.073159 0.054517 0.088606 0.091529 0.090217
Vacuole Periphery 0.051327 0.010506 0.000867 0.011758 0.013653 0.012866 0.000680 0.008877 0.021255 0.012181 0.000796 0.010507

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.40 2.61 -8.85 -9.05 -11.12 -2.27 5.33 -8.72 -8.10 -9.08 -2.11 4.28 -12.92 -12.25 -13.80
Bud Neck 1.52 0.82 -5.11 -8.86 -7.76 -9.85 2.84 -8.06 4.21 -10.71 -8.81 0.38 -10.98 0.29 -11.45
Bud Site 3.06 15.20 -0.32 -4.90 -30.67* -6.60 15.56 -20.20 -15.79 -28.99* -4.29 19.93 -20.63 -17.36 -42.02*
Cell Periphery 7.66 9.72 5.61 -4.46 -9.78 -0.96 0.28 -2.04 0.33 -2.07 -0.64 8.63 -1.88 0.27 -4.57
Cytoplasm -22.70 -52.15 -22.75 5.58 33.38 17.41 -2.06 11.29 -4.74 13.94 7.02 -19.22 7.77 0.42 34.29
Cytoplasmic Foci 9.98 31.58 24.33 16.58 -22.14 -9.58 34.23 16.85 27.33 -20.64 -4.02 42.92 25.84 32.84 -30.09
Eisosomes 8.47 11.72 4.18 -7.49 -14.91 -5.55 1.35 -5.14 2.10 -7.19 -1.93 9.20 -4.99 -2.00 -16.54
Endoplasmic Reticulum 4.34 5.14 4.96 2.59 -1.17 4.68 4.69 7.17 4.88 7.52 6.20 7.54 8.36 4.86 3.56
Endosome 6.42 15.71 8.80 2.51 -15.98 -13.08 14.19 -0.68 12.37 -14.30 -8.02 20.25 2.53 10.88 -21.06
Golgi 10.78 16.98 13.00 3.17 -12.86 -5.02 15.43 -2.12 1.49 -10.29 3.30 20.55 5.96 3.16 -16.38
Lipid Particles 1.71 1.95 0.35 -7.19 -8.79 1.98 2.83 -2.16 -4.48 -5.31 2.60 3.65 -1.72 -8.15 -10.26
Mitochondria 9.10 9.87 8.06 -4.59 -9.67 -3.20 9.68 3.44 6.37 -7.70 5.14 12.56 6.59 1.96 -11.82
Mitotic Spindle 1.50 3.54 -8.13 -10.41 -12.86 -2.69 -0.42 -11.51 -10.18 -11.26 -1.81 1.85 -15.57 -14.51 -17.15
None -0.44 -14.65 0.44 0.83 14.76 1.68 -10.96 8.61 2.85 14.26 1.80 -16.51 6.21 2.56 20.94
Nuclear Periphery 1.53 -7.98 -2.36 -2.80 -0.17 0.54 -11.78 -0.55 -1.26 12.38 1.18 -11.74 -2.04 -2.58 2.76
Nuclear Periphery Foci -0.78 1.09 0.03 1.31 -2.44 4.81 -5.92 0.29 -3.50 5.53 2.28 -1.14 0.16 -2.16 1.38
Nucleolus 0.09 -1.92 -0.12 -0.27 2.68 1.69 2.27 1.77 0.49 -0.50 1.79 1.81 2.13 1.10 1.03
Nucleus -6.30 -48.23 -11.68 -4.48 41.92 6.91 -35.44 4.50 -2.08 38.43 5.85 -51.46 2.73 -3.94 56.57
Peroxisomes 1.17 7.09 -7.70 -9.90 -15.42 -13.41 10.05 -10.87 -6.36 -12.27 -9.78 10.67 -16.15 -11.81 -19.65
Vacuole -4.98 -15.59 -21.17 -15.12 -8.51 -11.24 -16.54 -8.99 0.47 7.31 -11.24 -18.95 -19.51 -8.74 -2.14
Vacuole Periphery 8.52 10.87 9.01 0.93 -8.79 0.73 13.44 5.06 4.55 -6.66 6.40 16.32 9.45 4.13 -11.00
Standard name
Human Ortholog
Description Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Nit3

Nit3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nit3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available