Standard name
Human Ortholog
Description Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.19 0.14 0.09 0.11 0.07 0.1 0.12 0.08 0.1 0.05 0.08 0.1 0.1 0.1 0.3 0.2 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0 0.07 0.06 0.14 0.24 0.19 0.3 0.25 0.28 0.28 0.1 0.08 0 0.17 0.12 0.06 0 0 0 0 0 0
Nucleus 0.95 0.97 0.91 0.9 0.86 0.85 0.73 0.7 0.71 0.68 0.7 0.67 0.86 0.86 0.86 0.71 0.78 0.79 0.95 0.9 0.91 0.8 0.67 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.09 0 0.08 0.1 0.21 0.29 0.35 0.43 0.37 0.47 0.4 0.09 0.15 0.14 0.07 0 0.11 0 0 0 0.1 0.13 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0.05 0 0.06 0.17 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 7 18 29 25 43 21 45 55 29 39 16 27 21 22 23 46 25 5 2 0 2 1 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 10 19 4 21 22 43 110 87 112 99 93 100 21 17 10 25 15 5 0 0 0 1 0 0
Nucleus 227 91 193 254 346 257 340 323 267 266 230 240 183 182 205 107 100 63 228 89 205 119 111 138
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 13 8 7 22 39 63 134 160 163 145 154 142 19 32 34 10 5 9 0 0 6 14 21 32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 2 2 3 1 1 4 1 4 3 2 4 1 3 8 4 6 5 4 6 9 27 51
Unique Cell Count 238 94 213 282 400 303 463 462 377 389 330 357 212 212 239 151 128 80 240 100 226 150 167 232
Labelled Cell Count 260 136 235 325 454 385 630 629 572 553 496 511 248 254 278 198 153 89 240 100 226 150 167 232


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 62.8 104.5 66.0 70.9 70.7 60.9 60.0 60.0 50.8 54.1 47.2 57.2 89.8 107.4 104.9 127.5 147.2 134.7 72.4 78.0 84.2
Std Deviation (1e-4) 19.5 37.2 33.5 29.7 33.5 29.5 26.2 27.4 24.8 28.2 20.8 26.5 43.3 47.6 56.1 63.0 60.5 45.8 35.7 33.6 35.3
Intensity Change (Log2) – – – 0.1 0.1 -0.12 -0.14 -0.14 -0.38 -0.29 -0.48 -0.21 0.44 0.7 0.67 0.95 1.16 1.03 0.13 0.24 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -1.0 -2.5 -1.5 -0.6 -2.3 -1.3 -3.6 -2.3 -1.2 -1.0 -1.3 3.9 1.4 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 2.1 4.8 7.1 6.0 8.2 7.3 7.8 7.8 3.5 2.9 1.4 5.1 3.8 0
Nucleus -0.2 -1.5 -1.9 -5.1 -5.9 -5.6 -6.1 -5.7 -6.3 -1.4 -1.5 -1.6 -4.9 -3.2 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 2.9 5.7 7.6 8.8 10.3 9.2 10.8 9.6 2.4 4.2 4.0 1.5 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 92.0447 90.5635 90.2316 87.6353 74.9822 87.7511 100.8135 97.2981 92.7329 80.8065 58.259 83.5808 126.9663 129.2729 109.8007 97.4179 115.4343 118.74
Actin 0.0012 0 0.0015 0 0.0269 0.0061 0.0051 0 0.0026 0.0143 0.0302 0.006 0.0001 0 0 0 0 0
Bud 0.0003 0 0 0 0.0005 0.0001 0.0004 0 0.0001 0.0004 0.0009 0.0001 0 0 0 0 0 0
Bud Neck 0.0001 0 0 0 0.0008 0.0003 0.0001 0 0 0.0002 0.0003 0.0002 0 0 0 0 0 0
Bud Periphery 0.0003 0 0.0001 0 0.0007 0.0002 0.0011 0 0.0001 0.0003 0.0018 0.0001 0 0 0 0 0 0
Bud Site 0.001 0 0.0001 0 0.0044 0.0001 0.0007 0 0.0008 0.0014 0.0011 0.0001 0 0 0 0 0 0
Cell Periphery 0.0006 0.0003 0 0.0002 0.0004 0.0001 0.0003 0 0.0001 0.0001 0.0012 0.0002 0 0.0004 0 0 0.0001 0.0001
Cytoplasm 0.0035 0.0001 0.0003 0 0.0022 0.0004 0.0002 0 0.0001 0.0007 0.0013 0.0001 0.0006 0 0 0 0 0
Cytoplasmic Foci 0.0106 0 0.0012 0.0001 0.0041 0.0025 0.0318 0 0.0018 0.0071 0.0411 0.0131 0 0 0 0 0 0
Eisosomes 0.0001 0 0 0 0.0003 0 0.0005 0 0.0001 0.0001 0.0006 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0011 0 0.0008 0 0.0028 0.0003 0.0002 0 0 0.0003 0.0041 0.0001 0 0 0 0 0 0
Endosome 0.0124 0 0.0042 0 0.0139 0.0018 0.0018 0 0.001 0.011 0.0481 0.0042 0.0001 0 0 0 0 0
Golgi 0.0016 0 0.0006 0 0.0054 0.0025 0.0015 0 0.0011 0.0217 0.0432 0.0087 0 0 0 0 0 0
Lipid Particles 0.0048 0 0.0019 0.0001 0.0057 0.0019 0.0033 0 0.0019 0.0013 0.0319 0.0019 0 0 0 0 0 0
Mitochondria 0.0096 0.0004 0.0027 0.0058 0.0077 0.0017 0.0235 0.0005 0.0084 0.0148 0.0921 0.0058 0.0064 0.0044 0.006 0.0056 0.026 0.0025
None 0.0031 0 0.0004 0 0.0017 0.0015 0.0014 0 0.0004 0.0001 0.0025 0.0008 0.0001 0 0 0 0 0
Nuclear Periphery 0.0075 0.0011 0.003 0.0001 0.0118 0.0008 0.0009 0.0002 0.0002 0.0002 0.0141 0.0001 0.0087 0.0001 0.0001 0.0003 0.0001 0
Nucleolus 0.132 0.0273 0.0414 0.0841 0.1905 0.0671 0.0676 0.022 0.0278 0.0136 0.2603 0.1283 0.0788 0.0763 0.1002 0.0599 0.1889 0.1131
Nucleus 0.7972 0.9685 0.939 0.9081 0.7039 0.9081 0.8483 0.9772 0.9508 0.9091 0.3667 0.8284 0.9043 0.9142 0.8933 0.9334 0.7838 0.8839
Peroxisomes 0.0023 0 0.0005 0 0.0034 0.0012 0.009 0 0.0021 0.0029 0.026 0.0007 0 0 0 0 0 0
Punctate Nuclear 0.0069 0 0.0013 0 0.0058 0.0028 0.0005 0 0.0002 0.0001 0.0201 0.0001 0.0001 0 0 0 0 0
Vacuole 0.0017 0.0012 0.0004 0.0003 0.0026 0.0003 0.0006 0 0.0002 0.0002 0.0029 0.0003 0.0001 0.0008 0 0.0001 0.0001 0.0001
Vacuole Periphery 0.0021 0.001 0.0006 0.0011 0.0046 0.0004 0.0012 0.0001 0.0003 0.0002 0.0094 0.0005 0.0006 0.0037 0.0003 0.0006 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 549.5129 519.6089 443.6622 445.3034 478.9996 565.6193 569.652 539.2357 552.8445 549.8444
Translational Efficiency 1.8935 1.9659 1.9255 2.0582 2.1163 2.149 1.9265 2.0624 1.9912 1.9359

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2334 641 307 122 2178 1434 292 1363 4512 2075 599 1485

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4506.82 6341.32 7552.08 8615.51 4615.20 5371.18 7613.91 7725.54 4559.14 5670.87 7582.22 7798.66
Standard Deviation 1394.10 1892.39 1871.99 2429.07 1424.46 1675.91 1887.64 2425.41 1409.88 1802.28 1879.89 2437.99
Intensity Change Log 2 — 0.492673 0.744764 0.934826 — 0.218846 0.722244 0.743243 — 0.360624 0.733414 0.841075

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000560 0.000472 0.000513 0.000722 0.000577 0.000454 0.000554 0.000857 0.000568 0.000460 0.000533 0.000846
Bud Neck 0.000732 0.001317 0.002668 0.002855 0.000971 0.001054 0.002186 0.003187 0.000847 0.001135 0.002433 0.003160
Bud Site 0.000081 0.000170 0.000260 0.000255 0.000095 0.000121 0.000241 0.000382 0.000087 0.000136 0.000251 0.000371
Cell Periphery 0.000603 0.000193 0.000021 0.000026 0.000518 0.000602 0.000018 0.000186 0.000562 0.000475 0.000020 0.000173
Cytoplasm 0.000080 0.000255 0.000013 0.000058 0.000059 0.000173 0.000021 0.000586 0.000070 0.000198 0.000017 0.000543
Cytoplasmic Foci 0.000102 0.000841 0.000302 0.000630 0.000307 0.000403 0.000373 0.001896 0.000201 0.000538 0.000337 0.001792
Eisosomes 0.002071 0.000685 0.000304 0.000180 0.001637 0.001119 0.000316 0.000239 0.001861 0.000985 0.000310 0.000234
Endoplasmic Reticulum 0.000290 0.000125 0.000077 0.000073 0.000296 0.000205 0.000079 0.000208 0.000293 0.000180 0.000078 0.000197
Endosome 0.000001 0.000014 0.000001 0.000001 0.000002 0.000005 0.000001 0.000235 0.000001 0.000008 0.000001 0.000215
Golgi 0.000002 0.000015 0.000008 0.000012 0.000013 0.000013 0.000010 0.000350 0.000007 0.000014 0.000009 0.000322
Lipid Particles 0.000000 0.000003 0.000001 0.000002 0.000000 0.000001 0.000001 0.000014 0.000000 0.000002 0.000001 0.000013
Mitochondria 0.000542 0.000642 0.001171 0.001903 0.000615 0.000582 0.001278 0.002001 0.000577 0.000601 0.001223 0.001993
Mitotic Spindle 0.000000 0.000027 0.000001 0.000003 0.000003 0.000013 0.000002 0.000703 0.000002 0.000017 0.000001 0.000645
None 0.000664 0.000770 0.000284 0.000637 0.000520 0.000850 0.000416 0.000721 0.000594 0.000825 0.000348 0.000714
Nuclear Periphery 0.000012 0.000031 0.000012 0.000034 0.000022 0.000034 0.000014 0.000254 0.000017 0.000033 0.000013 0.000236
Nuclear Periphery Foci 0.000000 0.000014 0.000000 0.000001 0.000014 0.000032 0.000000 0.000290 0.000007 0.000026 0.000000 0.000267
Nucleolus 0.003926 0.008325 0.007930 0.012214 0.004708 0.003903 0.011935 0.017632 0.004304 0.005269 0.009883 0.017187
Nucleus 0.987234* 0.982912* 0.982388* 0.974459* 0.985670* 0.987509* 0.976092* 0.965836* 0.986479* 0.986089* 0.979319* 0.966544*
Peroxisomes 0.000496 0.001986 0.003832 0.005632 0.000661 0.000664 0.006214 0.002240 0.000576 0.001073 0.004993 0.002519
Vacuole 0.002342 0.000843 0.000139 0.000159 0.002470 0.001446 0.000152 0.000961 0.002404 0.001259 0.000145 0.000896
Vacuole Periphery 0.000260 0.000361 0.000075 0.000146 0.000843 0.000817 0.000098 0.001221 0.000542 0.000677 0.000086 0.001133

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.36 1.17 -1.62 -2.38 -1.98 4.03 0.42 -4.10 -6.02 -4.06 4.85 1.08 -4.54 -6.26 -4.84
Bud Neck -5.38 -9.81 -3.83 -2.73 -0.36 -1.11 -5.62 -11.59 -10.70 -3.74 -4.69 -10.80 -12.78 -10.83 -3.51
Bud Site -2.61 -5.88 -2.31 -0.87 0.05 -1.66 -3.70 -3.72 -3.43 -1.98 -3.27 -6.55 -3.95 -3.31 -1.99
Cell Periphery 3.30 6.51 6.45 2.02 -0.47 -0.36 3.06 1.89 2.26 -2.71 0.55 5.93 3.60 2.25 -2.69
Cytoplasm -1.17 2.67 0.85 1.29 -3.13 -2.32 2.43 -1.93 -1.70 -2.00 -2.00 3.51 -1.90 -1.56 -2.02
Cytoplasmic Foci -1.59 -4.41 -1.79 0.66 -1.10 -0.40 -0.36 -2.41 -2.28 -2.38 -1.72 -1.53 -2.65 -1.96 -2.47
Eisosomes 5.21 7.57 8.12 3.84 4.37 2.46 8.34 8.59 5.97 1.65 4.96 10.85 11.03 6.66 1.82
Endoplasmic Reticulum 5.84 10.61 9.88 2.31 0.38 3.28 9.07 2.23 -0.05 -3.86 5.77 13.84 2.89 -0.48 -3.91
Endosome -1.38 2.52 -0.19 1.37 -1.50 -1.47 2.49 -1.53 -1.51 -1.54 -1.95 3.54 -1.53 -1.48 -1.54
Golgi -1.70 -5.13 -2.68 0.61 -1.14 -0.05 0.33 -1.31 -1.31 -1.33 -1.05 -0.38 -1.34 -1.31 -1.33
Lipid Particles -1.41 -6.17 -3.84 0.96 -1.28 -1.03 -4.71 -1.94 -1.88 -1.82 -1.71 -7.51 -1.97 -1.72 -1.85
Mitochondria -2.24 -6.53 -4.34 -3.99 -2.24 0.81 -6.38 -10.12 -10.33 -4.33 -0.85 -9.13 -11.14 -10.86 -5.39
Mitotic Spindle -1.26 -3.86 -1.43 1.13 -0.97 -0.72 0.54 -1.72 -1.69 -1.72 -1.44 0.26 -1.72 -1.68 -1.72
None -0.32 2.54 0.12 0.39 -2.50 -1.06 0.95 -1.49 0.33 -2.44 -0.95 2.63 -1.00 0.37 -3.36
Nuclear Periphery -1.38 0.50 -1.76 -0.09 -1.83 -0.71 1.33 -3.23 -3.02 -3.36 -1.37 1.41 -3.33 -3.04 -3.40
Nuclear Periphery Foci -1.42 1.25 -0.96 1.36 -1.41 -0.53 1.05 -1.02 -0.95 -1.08 -0.94 1.07 -1.05 -0.96 -1.08
Nucleolus -2.67 -2.28 -2.50 -1.09 -1.19 1.18 -3.27 -6.23 -6.58 -1.97 -1.44 -3.97 -6.76 -6.06 -3.20
Nucleus 1.99 2.03 2.21 1.32 1.29 -1.20 2.90 6.54 6.96 2.57 0.46 3.53 7.20 6.73 3.99
Peroxisomes -4.52 -4.95 -2.84 -2.00 -0.95 -0.02 -4.77 -4.55 -4.40 3.21 -3.46 -6.64 -5.53 -3.99 3.19
Vacuole 4.47 9.59 9.46 2.89 -0.82 2.32 6.42 3.20 1.22 -2.87 4.03 10.71 4.38 1.07 -2.90
Vacuole Periphery -0.52 4.72 2.20 1.07 -2.07 0.04 1.76 -0.55 -0.53 -2.10 -0.31 2.21 -1.11 -0.73 -2.13
Standard name
Human Ortholog
Description Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Tal1

Tal1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tal1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available