Standard name
Human Ortholog
Description Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.25 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.99 0.99 0.98 0.95 0.94 0.93 0.89 0.87 0.78 0.89 0.98 1.0 0.99 0.95 0.9 0.94 0.94 0.91 0.88 0.76 0.73 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.06 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0.05 0.18 0.17 0.27 0.12 0 0 0 0 0.1 0 0 0 0 0 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 0 2 2 3 2 1 0 3 0 0 0 0 0 0 1 2 1 1 3 2
Bud 2 5 0 0 1 11 7 6 19 10 13 6 3 2 2 0 0 0 0 1 2 2 2 0
Bud Neck 0 0 3 1 6 3 4 4 1 0 0 9 0 0 0 0 0 0 2 3 4 3 3 3
Bud Site 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 7 55 18 14 16 6 8 7 11 11 6 2 9 5 3 2 4 0 0 1 1 2 2
Cytoplasm 334 431 570 436 454 603 589 635 546 539 417 497 194 229 205 20 18 15 337 411 520 114 166 131
Endoplasmic Reticulum 5 7 4 1 0 4 3 10 1 5 3 2 1 0 1 1 0 0 2 3 4 8 12 6
Endosome 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2 3 3 0 1 0
Golgi 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Mitochondria 2 8 0 9 4 34 35 32 110 107 141 69 7 0 0 0 2 0 4 9 22 5 15 7
Nucleus 1 1 0 1 4 4 3 6 0 3 7 6 1 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 5 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 4 1 0 0 5 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 0 2 2 4 3 4 5 3 16 2 2 1 0 0 1 0 2 10 22 7 13 8
Unique Cell Count 339 443 575 441 463 636 624 681 611 622 532 560 198 230 208 21 20 16 359 454 594 150 227 168
Labelled Cell Count 352 461 635 469 485 681 656 715 693 685 612 607 210 241 215 24 23 19 359 454 594 150 227 168


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.5 6.2 5.5 5.7 4.7 4.7 5.2 4.5 4.3 4.1 4.5 5.9 6.0 6.1 9.9 9.2 10.1 5.6 6.0 6.2
Std Deviation (1e-4) 0.7 0.8 0.7 0.9 0.9 0.7 0.7 1.0 1.1 1.0 0.9 0.7 1.3 1.1 1.4 1.0 1.2 1.8 2.1 3.4 2.4
Intensity Change (Log2) – – – -0.16 -0.11 -0.39 -0.4 -0.25 -0.47 -0.51 -0.58 -0.44 -0.08 -0.04 -0.02 0.67 0.58 0.7 -0.15 -0.05 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.4 -4.2 0 0 -6.8 0 0 0 0 -4.0 -2.7 -3.3 0 0 0
Cytoplasm -0.4 -1.5 -4.3 -4.6 -5.3 -7.1 -8.3 -11.1 -7.4 -1.3 0.6 -0.7 -1.8 -3.7 -2.1
Endoplasmic Reticulum 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 5.3 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8513 1.3266 1.0775 1.065 1.0235 1.1057 0.9282 1.8903 1.4347 1.2095 0.9523 1.5206 1.0084 1.5522 0.847 1.0082 0.8383 0.9302
Actin 0.0136 0.0003 0.0099 0.0003 0.0164 0.0061 0.0159 0.0011 0.0063 0.0182 0.0141 0.0041 0.0572 0.005 0.0341 0.0084 0.025 0.0047
Bud 0.0017 0.0044 0.0031 0.0193 0.0008 0.0003 0.001 0.0042 0.0021 0.001 0.0064 0.0011 0.0014 0.0043 0.0011 0.0005 0.0106 0.0006
Bud Neck 0.0067 0.0003 0.001 0.0004 0.0074 0.0055 0.0026 0.0005 0.0007 0.0011 0.0019 0.0083 0.0023 0.0006 0.0017 0.0011 0.0009 0.0025
Bud Periphery 0.0028 0.0007 0.0012 0.0091 0.0011 0.0005 0.0011 0.004 0.002 0.0009 0.0093 0.001 0.0024 0.0054 0.0012 0.0004 0.0347 0.0006
Bud Site 0.0067 0.0079 0.0161 0.0011 0.0039 0.0012 0.0057 0.0233 0.0102 0.0011 0.032 0.0005 0.0061 0.0177 0.0087 0.002 0.0027 0.0006
Cell Periphery 0.0006 0.0003 0.0005 0.0004 0.0003 0.0002 0.0005 0.0007 0.0004 0.0002 0.0003 0.0001 0.0007 0.0007 0.0005 0.0002 0.0004 0.0001
Cytoplasm 0.5753 0.7809 0.651 0.6441 0.6356 0.7766 0.7589 0.831 0.7977 0.6939 0.7524 0.8651 0.513 0.7737 0.641 0.6278 0.6659 0.736
Cytoplasmic Foci 0.0379 0.0159 0.028 0.0164 0.0218 0.0257 0.0337 0.0157 0.0245 0.0463 0.0456 0.0218 0.0452 0.0203 0.0271 0.0592 0.0423 0.0296
Eisosomes 0.0003 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0 0 0.0004 0.0001 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0111 0.0036 0.0056 0.0027 0.0047 0.0037 0.004 0.0021 0.0032 0.0047 0.0055 0.0016 0.0079 0.0021 0.0051 0.0052 0.0025 0.0019
Endosome 0.0403 0.015 0.0295 0.0246 0.061 0.0193 0.0184 0.0104 0.0141 0.1003 0.0254 0.0125 0.0336 0.0082 0.0266 0.0434 0.0244 0.0163
Golgi 0.0086 0.0008 0.0042 0.0005 0.0061 0.0041 0.0063 0.0011 0.0022 0.0056 0.0029 0.0012 0.0102 0.0013 0.0059 0.0088 0.0079 0.0022
Lipid Particles 0.0087 0.0004 0.0032 0.0003 0.0014 0.0086 0.0037 0.0001 0.0016 0.002 0.0004 0.0011 0.0123 0.0003 0.0027 0.0359 0.0286 0.0009
Mitochondria 0.0076 0.0004 0.0032 0.0005 0.004 0.0017 0.0017 0.0006 0.0035 0.0011 0.0022 0.0008 0.0072 0.0005 0.0113 0.0035 0.009 0.005
None 0.2329 0.1579 0.2178 0.2678 0.1621 0.1343 0.1265 0.0982 0.1048 0.098 0.0884 0.0731 0.2564 0.1531 0.2163 0.1692 0.1321 0.1884
Nuclear Periphery 0.0106 0.0008 0.0028 0.0011 0.0307 0.0007 0.0031 0.0003 0.0012 0.004 0.0006 0.0003 0.0046 0.0004 0.0018 0.0021 0.0011 0.0007
Nucleolus 0.0014 0.0005 0.0006 0.0002 0.0007 0.0013 0.0003 0.0001 0.0006 0.0002 0.0001 0.0001 0.0007 0.0001 0.0002 0.0014 0.0001 0.0002
Nucleus 0.0055 0.0022 0.0032 0.003 0.0147 0.001 0.0032 0.0012 0.0046 0.0019 0.0013 0.0009 0.0022 0.001 0.0011 0.0013 0.001 0.0011
Peroxisomes 0.0111 0.0001 0.0063 0.0001 0.0033 0.0036 0.0062 0.0012 0.012 0.0004 0.0046 0.003 0.0208 0.0012 0.0056 0.0202 0.0049 0.0048
Punctate Nuclear 0.0048 0.001 0.0061 0.0009 0.002 0.001 0.002 0.0003 0.0025 0.0015 0.0012 0.0007 0.0043 0.0006 0.0009 0.003 0.0017 0.0012
Vacuole 0.0096 0.0062 0.0054 0.0061 0.0177 0.0037 0.0043 0.0033 0.0051 0.0154 0.0046 0.0024 0.0092 0.0032 0.0055 0.0053 0.0032 0.0021
Vacuole Periphery 0.0023 0.0005 0.0011 0.0009 0.0042 0.0006 0.0005 0.0005 0.0007 0.0025 0.0008 0.0004 0.0019 0.0004 0.0011 0.0013 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.4523 4.1213 6.1164 11.2063 8.6341 9.7275 12.2698 10.625 11.8264 10.1856
Translational Efficiency 1.1389 1.8204 1.568 0.7981 0.8147 1.179 0.8823 1.0543 0.9332 1.0105

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
558 321 320 1102 1481 1351 213 123 2039 1672 533 1225

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 730.34 904.21 1067.12 958.20 693.75 820.74 1036.39 1010.49 703.76 836.77 1054.84 963.45
Standard Deviation 77.76 108.65 118.22 133.45 101.14 103.99 123.45 126.13 96.70 109.93 121.28 133.66
Intensity Change Log 2 — 0.308090 0.547082 0.391759 — 0.242509 0.579079 0.542567 — 0.276514 0.562758 0.467196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000201 0.000708 0.000175 0.001137 0.000323 0.000887 0.000402 0.004699 0.000289 0.000852 0.000266 0.001495
Bud Neck 0.031325 0.013624 0.002262 0.005155 0.019696 0.022841 0.001903 0.002259 0.022878 0.021072 0.002118 0.004864
Bud Site 0.021206 0.031970 0.007559 0.045833 0.020361 0.037304 0.008504 0.033520 0.020592 0.036280 0.007937 0.044597
Cell Periphery 0.000388 0.000219 0.000153 0.000111 0.000184 0.000207 0.000189 0.000152 0.000240 0.000209 0.000167 0.000115
Cytoplasm 0.506522* 0.473294* 0.391924* 0.509963* 0.475925* 0.442334* 0.527281* 0.469829* 0.484298* 0.448278* 0.446016* 0.505933*
Cytoplasmic Foci 0.263844* 0.277887* 0.055990 0.080710 0.263365* 0.283600* 0.053614 0.081911 0.263496* 0.282503* 0.055040 0.080831
Eisosomes 0.000425 0.000181 0.000077 0.000027 0.000261 0.000208 0.000075 0.000054 0.000306 0.000203 0.000076 0.000030
Endoplasmic Reticulum 0.001126 0.000927 0.001737 0.000527 0.002042 0.000897 0.001852 0.000488 0.001792 0.000903 0.001783 0.000523
Endosome 0.039729 0.044422 0.013625 0.018981 0.046286 0.060920 0.008341 0.011807 0.044492 0.057753 0.011513 0.018260
Golgi 0.008357 0.007909 0.000152 0.008142 0.012448 0.019744 0.000180 0.021164 0.011328 0.017472 0.000163 0.009450
Lipid Particles 0.007456 0.002220 0.002650 0.000668 0.007491 0.004112 0.002118 0.000693 0.007481 0.003749 0.002438 0.000670
Mitochondria 0.003325 0.004761 0.000146 0.001184 0.004318 0.005409 0.000105 0.000081 0.004047 0.005285 0.000130 0.001073
Mitotic Spindle 0.000078 0.001624 0.001635 0.037883 0.000593 0.007326 0.002454 0.002429 0.000452 0.006232 0.001962 0.034323
None 0.000384 0.000477 0.006708 0.001614 0.001367 0.000697 0.002013 0.000360 0.001098 0.000655 0.004832 0.001488
Nuclear Periphery 0.000092 0.000086 0.000768 0.000212 0.000206 0.000169 0.000591 0.000134 0.000175 0.000154 0.000697 0.000204
Nuclear Periphery Foci 0.000260 0.000956 0.004540 0.001219 0.000361 0.000267 0.005443 0.002406 0.000333 0.000400 0.004901 0.001338
Nucleolus 0.000266 0.000464 0.000409 0.000169 0.000558 0.000488 0.000189 0.000067 0.000478 0.000483 0.000321 0.000159
Nucleus 0.017623 0.015028 0.076675 0.012073 0.021507 0.008567 0.061624 0.007609 0.020444 0.009807 0.070660 0.011625
Peroxisomes 0.003667 0.009316 0.000518 0.003776 0.004293 0.007460 0.000415 0.002305 0.004122 0.007816 0.000476 0.003628
Vacuole 0.090024 0.111866 0.431831* 0.266313* 0.113779 0.092363 0.322522* 0.357873* 0.107278 0.096108 0.388149* 0.275506*
Vacuole Periphery 0.003702 0.002060 0.000468 0.004302 0.004636 0.004198 0.000186 0.000160 0.004381 0.003788 0.000355 0.003886

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.78 0.05 -2.70 0.37 -2.73 -1.94 -0.48 -0.93 -0.26 -0.89 -2.37 0.99 -2.30 0.66 -2.50
Bud Neck 6.06 11.80 11.27 5.85 -2.14 -1.62 14.58 14.18 13.41 -0.10 1.10 18.50 16.81 13.42 -2.58
Bud Site -2.53 6.32 -1.52 1.30 -7.14 -6.85 9.78 0.31 4.26 -3.18 -7.27 10.88 -1.96 3.78 -7.89
Cell Periphery 3.67 7.65 9.76 4.12 4.85 -1.17 2.47 5.71 5.58 3.20 1.67 7.39 12.95 8.37 6.25
Cytoplasm 1.89 8.27 2.32 -0.21 -6.71 3.35 2.39 3.12 1.74 0.90 4.16 7.47 1.59 -1.87 -5.66
Cytoplasmic Foci -0.85 28.11 26.90 18.66 -3.85 -2.75 36.85 28.94 29.88 -2.82 -2.89 46.53 42.69 39.84 -5.23
Eisosomes 5.32 9.47 10.81 5.46 9.17 1.91 8.99 10.59 9.87 5.26 4.35 12.59 15.19 11.82 12.02
Endoplasmic Reticulum 0.63 -3.43 4.18 2.19 11.67 2.56 0.34 4.25 2.92 7.34 2.62 -0.35 4.89 3.46 13.34
Endosome -0.67 11.48 11.53 6.41 1.24 -4.54 19.39 16.90 19.02 1.01 -4.70 21.09 18.52 19.04 0.23
Golgi 0.40 13.34 4.83 2.50 -3.22 -4.88 17.31 1.93 3.54 -0.99 -4.92 20.56 7.62 9.82 -3.32
Lipid Particles 5.56 5.56 7.52 4.37 7.60 4.63 8.53 10.56 9.62 6.31 6.23 9.79 12.82 10.33 9.62
Mitochondria -0.40 3.33 2.93 1.32 -1.87 -1.01 7.86 7.95 5.45 2.11 -1.13 8.34 7.27 4.78 -1.95
Mitotic Spindle -2.25 -3.08 -6.31 -5.73 -5.49 -4.58 -1.82 -1.60 -0.12 -0.52 -4.84 -2.68 -6.36 -4.05 -4.99
None -0.47 -1.46 -1.85 -1.55 1.13 2.11 -0.43 0.94 -0.82 1.34 1.85 -1.28 -0.15 -1.23 1.21
Nuclear Periphery 0.15 -14.57 -9.55 -8.36 10.95 0.50 -8.22 -1.78 -2.07 7.61 0.36 -13.53 -3.36 -2.94 13.50
Nuclear Periphery Foci -3.63 -14.07 -10.31 -1.10 10.91 2.18 -9.94 -5.00 -5.30 5.48 -1.46 -16.95 -10.53 -8.97 12.95
Nucleolus -0.66 -1.35 3.99 1.06 4.19 0.12 5.74 6.66 2.78 2.65 -0.29 4.03 6.25 2.65 3.77
Nucleus 1.02 -9.92 -0.84 -1.64 9.74 9.24 -7.07 2.97 -2.69 7.97 9.40 -11.78 1.58 -8.36 12.33
Peroxisomes -3.58 8.69 2.88 4.55 -5.29 -5.24 12.79 4.37 7.66 -1.57 -6.49 15.00 5.03 9.47 -5.65
Vacuole -3.16 -22.77 -28.98 -22.32 5.90 4.62 -15.44 -14.00 -14.82 -1.53 2.51 -26.91 -31.83 -32.52 5.20
Vacuole Periphery 2.80 7.34 2.90 0.53 -2.26 0.80 12.45 12.60 10.82 3.18 1.37 13.99 5.00 3.88 -2.40
Standard name
Human Ortholog
Description Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ste11

Ste11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available