Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.11 0 0 0.06 0.05 0 0.06 0 0.12 0.06 0.08 0.08 0.11 0.13 0.16 0 0 0 0.06 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.38 0.41 0.47 0.36 0.39 0.31 0.24 0.39 0.34 0.44 0.33 0.31 0.34 0.42 0.06 0.09 0.09 0 0 0 0 0 0
Nucleus 0.34 0.29 0.13 0.08 0.08 0.08 0 0.1 0.11 0.06 0.06 0.08 0.24 0.18 0.15 0.16 0.2 0.21 0.31 0.27 0.13 0.12 0.07 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.72 0.72 0.76 0.83 0.87 0.82 0.84 0.81 0.85 0.81 0.81 0.73 0.79 0.86 0.81 0.78 0.69 0.69 0.55 0.6 0.74 0.65 0.65 0.62
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0.09 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 4 1 3 8 5
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 2 0 0 1 2
Bud Site 0 1 0 0 0 2 0 0 1 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Cytoplasm 16 22 8 16 4 7 16 8 6 9 0 10 14 10 18 94 90 99 0 0 0 17 18 11
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 23 33 24 1 2 0 1 0 1
Endosome 1 0 0 0 0 2 1 4 0 3 0 0 0 1 2 3 2 2 1 1 0 1 1 5
Golgi 0 2 1 0 0 0 0 1 0 1 0 0 1 0 0 2 5 5 0 0 0 0 1 6
Mitochondria 162 266 42 67 42 72 77 40 67 52 7 27 78 44 94 54 64 54 2 3 0 0 10 11
Nucleus 152 200 13 12 10 14 11 17 18 10 1 7 59 23 33 139 141 132 140 186 14 35 23 30
Nuclear Periphery 0 4 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 6 9 1 1 2 5
Nucleolus 327 501 78 120 103 150 210 137 145 124 13 61 199 111 180 695 492 435 250 420 79 190 210 211
Peroxisomes 0 0 0 0 0 1 6 3 0 1 0 1 0 1 1 11 6 5 0 0 0 0 0 1
SpindlePole 0 2 0 0 0 6 8 7 3 12 0 6 0 1 1 6 4 3 1 1 0 1 2 3
Vac/Vac Membrane 10 7 3 1 2 5 1 2 3 4 0 2 3 0 3 19 22 24 31 36 4 26 31 30
Unique Cell Count 452 694 102 144 118 183 249 169 171 154 16 83 251 129 222 889 708 633 458 696 108 292 325 340
Labelled Cell Count 668 1007 145 216 161 260 332 220 246 216 21 115 354 191 332 1047 859 784 458 696 108 292 325 340


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 77.2 78.7 80.2 68.2 28.9 30.1 30.0 26.7 23.1 25.4 25.0 19.1 82.8 79.1 71.6 63.9 86.2 91.3 68.1 77.5 77.8
Std Deviation (1e-4) 33.3 31.9 36.7 43.4 17.0 16.3 18.2 16.4 12.9 18.0 10.6 12.8 31.6 34.3 33.7 38.2 47.0 47.6 30.6 38.4 33.4
Intensity Change (Log2) – – – -0.23 -1.47 -1.41 -1.42 -1.58 -1.8 -1.66 -1.68 -2.07 0.05 -0.02 -0.16 -0.33 0.11 0.19 -0.24 -0.05 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -1.5 -1.5 -0.5 -1.1 -1.6 -0.6 0 1.0 -0.8 0 0.1 0.9 1.4 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 -0.9 -0.3 -1.8 -3.0 -0.3 -1.2 0.2 -1.2 -1.8 -1.1 0.2 -11.4 -9.0 -9.1
Nucleus -1.1 -1.0 -1.4 -2.8 -0.7 -0.6 -1.7 0 -0.9 2.3 1.1 0.5 0.8 1.7 1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.3 2.1 1.1 1.7 0.9 1.7 0.8 0.4 -0.5 0.6 1.9 1.0 0.4 -1.4 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.8018 82.768 75.8932 72.489 75.856 68.6493 65.4706 94.8127 85.4162 88.7332 83.1427 88.2157 97.2082 104.8959 100.0754 94.2664 91.8537 94.4981
Actin 0.0573 0 0.0122 0 0 0.0019 0.0259 0 0.0007 0 0 0 0.001 0 0 0.0003 0.0001 0.0011
Bud 0.0006 0 0.0007 0 0 0.0007 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0022 0 0.0002 0 0 0.0002 0.0028 0 0 0 0.0001 0 0 0 0 0 0 0.0001
Bud Periphery 0.0009 0.0001 0.0029 0 0 0.0014 0.0005 0 0.0002 0 0.0001 0.0001 0 0 0 0 0 0.0001
Bud Site 0.0066 0.0001 0.0022 0 0 0.0004 0.0016 0.0001 0.0002 0 0.0004 0 0.0001 0 0 0.0001 0 0
Cell Periphery 0.0005 0.0001 0.0002 0.0001 0.0004 0.0003 0.0006 0.0003 0.0003 0.0001 0.0039 0.0007 0.0004 0.0002 0.0001 0.0002 0.0004 0.0003
Cytoplasm 0.0058 0 0.0016 0 0.0003 0.0006 0.0121 0.0005 0.0014 0 0.0027 0.0009 0 0 0.0001 0.016 0 0.0001
Cytoplasmic Foci 0.0174 0 0.0044 0 0.0492 0.0032 0.005 0.009 0.0167 0 0.0129 0.0025 0.0013 0 0.0051 0.005 0.0002 0.0059
Eisosomes 0.0009 0 0.0001 0 0.0003 0 0.001 0 0.0002 0 0.0001 0 0.0001 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.0037 0 0.0019 0 0 0.0012 0.0079 0 0.0012 0 0.0001 0 0 0 0 0.0015 0 0.0004
Endosome 0.0236 0 0.0113 0 0.0001 0.0105 0.0271 0.0011 0.0114 0 0.0001 0 0.0005 0 0 0.0018 0 0.0034
Golgi 0.0113 0 0.0025 0 0.0001 0.0043 0.019 0 0.0013 0 0 0 0.0006 0 0 0.0001 0 0.0016
Lipid Particles 0.0194 0 0.0014 0 0.0116 0.0011 0.002 0.0005 0.0021 0 0.0049 0.0005 0.0033 0 0.0005 0.0017 0.0003 0.001
Mitochondria 0.0128 0.001 0.0139 0.0016 0.0229 0.0089 0.0087 0.006 0.0054 0.0003 0.0031 0.0017 0.0044 0.0003 0.0016 0.0105 0.0107 0.0019
None 0.0085 0 0.001 0 0.0031 0.0002 0.0095 0.0008 0.002 0 0.0015 0.0011 0.0001 0 0.0007 0.003 0.0001 0.0003
Nuclear Periphery 0.0129 0.0001 0.0031 0 0.0001 0.0249 0.0309 0.0004 0.019 0 0.0006 0.0005 0.0001 0.0005 0.0002 0.0054 0.001 0.0009
Nucleolus 0.644 0.7495 0.7766 0.796 0.7812 0.7626 0.7125 0.725 0.7675 0.8058 0.6532 0.813 0.8591 0.6963 0.8172 0.7877 0.8529 0.8164
Nucleus 0.1314 0.2478 0.1605 0.2019 0.1251 0.1729 0.1259 0.2385 0.1599 0.1937 0.2998 0.1753 0.1262 0.3021 0.1733 0.1621 0.1307 0.1638
Peroxisomes 0.0105 0 0.0006 0 0.002 0.0003 0.0007 0.0003 0.0004 0 0.0002 0.0001 0.0007 0 0.0001 0.0007 0.0001 0.0003
Punctate Nuclear 0.0212 0.0001 0.0007 0 0.0003 0.0006 0.0025 0.0002 0.0032 0 0.0005 0.0007 0.0001 0 0.0001 0.0025 0 0.0003
Vacuole 0.0058 0.0003 0.0011 0.0001 0.0008 0.0015 0.0018 0.0107 0.0023 0.0001 0.0106 0.0009 0.0007 0.0002 0.0002 0.0004 0.0003 0.0005
Vacuole Periphery 0.0028 0.0008 0.001 0.0003 0.0025 0.0021 0.002 0.0066 0.0044 0.0001 0.005 0.0019 0.0012 0.0003 0.0006 0.0009 0.0028 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 467.4729 512.0121 594.1141 496.4125 396.7855 390.0466 420.6271 398.2237 388.4701 396.7554
Translational Efficiency 1.6895 1.9159 1.6117 1.5662 2.3102 2.22 2.0049 1.8316 1.8628 2.177

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
597 1248 2885 1790 1857 2368 2382 2215 2454 3616 5267 4005

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2316.16 5440.11 5888.81 7245.52 4232.18 5381.76 6946.51 7419.29 3766.06 5401.90 6367.15 7341.63
Standard Deviation 996.26 1598.52 2019.73 2897.46 1388.09 1674.67 2362.52 2781.20 1541.22 1649.02 2244.06 2835.07
Intensity Change Log 2 — 1.231901 1.346241 1.645354 — 0.346677 0.714887 0.809880 — 0.724749 0.970918 1.163157

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000498 0.000529 0.000774 0.001841 0.000542 0.000584 0.001365 0.002016 0.000531 0.000565 0.001041 0.001938
Bud Neck 0.002336 0.001337 0.002830 0.004144 0.000998 0.001497 0.002948 0.003525 0.001323 0.001442 0.002883 0.003802
Bud Site 0.003609 0.000314 0.000401 0.000875 0.000187 0.000301 0.000459 0.000974 0.001020 0.000305 0.000427 0.000930
Cell Periphery 0.000996 0.000063 0.000050 0.000179 0.000020 0.000049 0.000093 0.000158 0.000258 0.000054 0.000069 0.000168
Cytoplasm 0.006298 0.000144 0.000151 0.001001 0.000035 0.000696 0.000127 0.000578 0.001559 0.000505 0.000140 0.000767
Cytoplasmic Foci 0.005446 0.003108 0.001416 0.003796 0.001984 0.002579 0.002283 0.003232 0.002827 0.002762 0.001808 0.003484
Eisosomes 0.000307 0.000596 0.000429 0.001057 0.000648 0.000570 0.001173 0.002497 0.000565 0.000579 0.000766 0.001853
Endoplasmic Reticulum 0.000155 0.000058 0.000102 0.000291 0.000057 0.000075 0.000154 0.000268 0.000081 0.000069 0.000125 0.000278
Endosome 0.000232 0.000004 0.000022 0.000198 0.000004 0.000009 0.000011 0.000089 0.000059 0.000008 0.000017 0.000138
Golgi 0.000155 0.000034 0.000050 0.000412 0.000033 0.000039 0.000043 0.000093 0.000062 0.000037 0.000047 0.000235
Lipid Particles 0.000325 0.000005 0.000017 0.000087 0.000006 0.000038 0.000008 0.000166 0.000084 0.000026 0.000013 0.000131
Mitochondria 0.000842 0.000886 0.001375 0.003349 0.000846 0.000893 0.001621 0.002571 0.000845 0.000891 0.001486 0.002918
Mitotic Spindle 0.000058 0.000481 0.000418 0.001183 0.000092 0.000250 0.001003 0.000743 0.000084 0.000329 0.000683 0.000940
None 0.015355 0.000699 0.000646 0.001510 0.000410 0.000591 0.000693 0.001466 0.004046 0.000628 0.000667 0.001486
Nuclear Periphery 0.000039 0.000026 0.000461 0.001230 0.000047 0.000038 0.000184 0.000396 0.000045 0.000034 0.000336 0.000768
Nuclear Periphery Foci 0.001882 0.000019 0.000058 0.000429 0.000026 0.000036 0.000033 0.000295 0.000477 0.000030 0.000047 0.000355
Nucleolus 0.426921* 0.517471* 0.414564* 0.503610* 0.536048* 0.446813* 0.429727* 0.479878* 0.509500* 0.471199* 0.421421* 0.490485*
Nucleus 0.503782* 0.431283* 0.560691* 0.441140* 0.410576* 0.517111* 0.540707* 0.464579* 0.433251* 0.487489* 0.551653* 0.454103*
Peroxisomes 0.027618 0.040945 0.013880 0.011787 0.045641 0.026089 0.010733 0.010796 0.041256 0.031216 0.012457 0.011239
Vacuole 0.002278 0.000649 0.000337 0.005044 0.000589 0.000571 0.002151 0.007114 0.001000 0.000598 0.001157 0.006189
Vacuole Periphery 0.000869 0.001348 0.001328 0.016838 0.001211 0.001172 0.004483 0.018568 0.001128 0.001233 0.002755 0.017795

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.88 -8.25 -18.63 -19.10 -15.48 -1.97 -21.45 -20.60 -20.08 -8.39 -1.86 -21.78 -27.81 -27.46* -17.11
Bud Neck 1.26 -0.61 -2.23 -8.02 -3.84 -5.57 -11.52 -8.25 -6.85 -2.04 -0.48 -7.05 -8.45 -10.41 -4.15
Bud Site 2.15 2.10 1.72 -2.50 -2.22 -2.68 -4.84 -2.39 -2.11 -1.61 1.90 1.52 -0.09 -3.03 -2.46
Cell Periphery 1.77 1.82 1.57 -2.18 -3.62 -2.32 -5.69 -4.61 -3.52 -2.16 1.57 1.49 0.69 -4.21 -4.25
Cytoplasm 2.94 2.96 2.58 -3.30 -3.64 -1.72 -3.40 -3.24 0.15 -2.67 1.92 2.79 1.58 -0.91 -4.42
Cytoplasmic Foci 1.86 3.32 0.87 -0.95 -2.61 -1.33 -0.91 -2.23 -1.20 -1.18 0.14 1.99 -1.48 -1.59 -2.89
Eisosomes -3.43 -1.44 -5.27 -3.66 -5.14 1.35 -4.20 -8.16 -8.72 -5.36 -0.21 -2.84 -9.13 -9.45 -7.58
Endoplasmic Reticulum 3.47 1.92 -1.65 -2.98 -2.42 -3.16 -11.65 -11.35 -10.08 -5.74 1.48 -5.57 -5.37 -5.76 -4.21
Endosome 1.81 1.66 0.26 -1.62 -1.46 -1.72 -3.36 -2.21 -2.07 -2.05 1.68 1.35 -1.24 -2.34 -2.16
Golgi 1.49 1.28 -0.81 -1.26 -1.21 -0.83 -2.86 -2.52 -2.13 -2.03 1.18 0.71 -1.30 -1.50 -1.43
Lipid Particles 1.41 1.36 1.07 -1.82 -1.43 -0.92 -0.60 -1.50 -1.16 -1.49 0.95 1.27 -0.58 -1.53 -1.82
Mitochondria -0.56 -5.38 -11.63 -11.56 -8.89 -0.71 -8.64 -9.12 -9.00 -4.75 -0.87 -9.56 -14.53 -14.44 -9.62
Mitotic Spindle -1.55 -2.36 -1.98 -1.14 -1.17 -1.21 -2.82 -1.97 -1.46 0.54 -1.93 -3.60 -2.75 -1.88 -0.61
None 3.89 3.90 3.66 -2.46 -2.71 -2.71 -2.82 -3.03 -2.46 -2.15 3.69 3.63 2.67 -3.52 -3.33
Nuclear Periphery 2.13 -1.32 -4.88 -4.93 -1.90 1.15 -4.77 -9.44 -9.78 -4.63 1.90 -1.65 -6.54 -6.64 -2.08
Nuclear Periphery Foci 2.60 2.54 2.03 -3.61 -3.18 -0.72 -1.61 -2.16 -2.05 -2.10 2.54 2.45 0.59 -3.62 -3.44
Nucleolus -5.94 0.82 -4.89 1.40 -8.62 10.03 11.37 6.00 -3.44 -5.06 4.98 11.91 2.59 -2.44 -9.73
Nucleus 4.49 -3.89 3.82 -0.97 11.52 -11.37 -13.30 -5.48 5.49 7.61 -6.75 -15.39 -2.67 4.36 13.66
Peroxisomes -5.62 7.79 8.90 15.81 2.62 10.47 19.92 20.40 14.10 0.19 6.41 20.99 22.02 20.85 2.34
Vacuole 2.36 2.89 -3.10 -8.53 -9.47 0.25 -3.94 -9.65 -10.14 -7.17 1.67 -0.29 -10.63 -13.08 -11.63
Vacuole Periphery -3.71 -4.62 -12.66 -12.27 -12.31 0.49 -10.70 -14.15 -14.22 -11.20 -1.08 -10.44 -18.88 -18.81 -16.89
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (46%), nucleus (9%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rps22b

Rps22b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps22b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available