Standard name
Human Ortholog
Description Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.91 0.76 0.97 0.95 0.93 0.85 0.85 0.87 0.69 0.75 0.57 0.56 0.9 0.91 0.82 – – – 0.67 0.65 0.73 0.81 0.81 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.18 0 0 0 0.07 0.12 0.1 0.34 0.34 0.54 0.51 0 0 0 – – – 0.06 0.05 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.09 0.07 0 0 0 – – – 0.05 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.07 0.05 0.05 0.08 0.09 0.07 0 0.07 0 0 0 0.05 0.05 0.16 – – – 0.14 0.16 0.07 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 2 1
Bud 0 0 0 0 0 0 1 0 3 2 6 2 0 0 0 – – – 0 0 0 0 1 5
Bud Neck 0 0 1 0 1 4 2 6 3 2 1 1 1 4 0 – – – 0 0 0 0 2 3
Bud Site 0 1 0 0 0 0 0 0 0 0 2 2 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 1 2 1 1 – – – 0 0 0 0 0 1
Cytoplasm 32 58 177 204 225 216 259 308 184 200 105 114 177 320 314 – – – 67 68 154 151 190 268
Endoplasmic Reticulum 0 1 0 1 0 0 0 0 0 0 0 1 3 1 4 – – – 0 0 1 0 3 0
Endosome 0 0 1 0 2 2 1 0 1 0 1 0 1 3 17 – – – 0 0 0 1 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 – – – 1 0 0 0 3 2
Mitochondria 0 14 0 1 0 17 38 35 92 92 98 105 0 1 0 – – – 5 5 19 4 6 11
Nucleus 1 3 0 2 3 6 6 10 10 14 17 15 5 9 3 – – – 5 7 5 6 4 5
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 4 5 4 3 2 2 0 3 1 – – – 2 3 4 4 1 15
Vac/Vac Membrane 2 5 9 10 20 22 22 15 19 9 1 3 10 17 63 – – – 13 16 15 10 11 11
Unique Cell Count 35 76 183 214 242 255 304 355 268 268 183 205 197 352 382 101 105 212 187 234 341
Labelled Cell Count 36 82 188 218 252 269 333 380 320 322 234 248 199 360 404 101 105 212 187 234 341


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.2 4.8 4.7 5.5 4.6 4.3 4.5 4.3 4.1 4.1 4.1 4.7 4.9 4.9 4.1 4.8 4.8 5.1 5.4 5.4
Std Deviation (1e-4) 0.9 0.4 0.8 1.1 2.0 1.7 1.6 1.4 2.3 1.8 2.0 1.9 1.0 1.0 1.4 1.4 1.4 1.4 1.4 1.7 1.5
Intensity Change (Log2) – – – -0.03 0.2 -0.09 -0.18 -0.1 -0.18 -0.22 -0.24 -0.25 -0.03 0.02 0.01 -0.23 -0.02 -0.02 0.06 0.16 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2095 -0.5582 -0.7823 -1.2447 -0.701 -0.7929 1.0003 0.8194 0.6934 0.6538 1.6283 0.9309 -1.5602 -1.1923 -1.4314 -1.3244 -1.5437 -2.0253
Actin 0.0087 0.0001 0.0016 0.0018 0.0025 0.0009 0.0315 0.0002 0.0015 0.0216 0.0341 0.0022 0.0719 0.0002 0.0221 0.0006 0.0097 0.0001
Bud 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0006 0.0002 0.0025 0.0007 0.0007 0.0003 0.0005 0.0001 0.0009 0.0252 0.0066 0
Bud Neck 0.0015 0.0002 0.0014 0.0007 0.0005 0.0015 0.0081 0 0.0004 0.0004 0.0072 0.001 0.0007 0.0001 0.0004 0.0004 0.0097 0.0009
Bud Periphery 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.001 0.0007 0.0061 0.0005 0.0009 0.0003 0.0005 0 0.002 0.0149 0.0209 0
Bud Site 0.0007 0.001 0.0014 0.0002 0.0001 0.0001 0.0079 0.0002 0.0011 0.0046 0.0282 0.0009 0.0017 0.0008 0.0054 0.0022 0.0321 0
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0005 0.0001 0.0001 0.0001 0.0009 0 0.0002 0.0001 0.0001 0.0002 0.0018 0
Cytoplasm 0.1401 0.1306 0.1667 0.2111 0.2082 0.239 0.0972 0.0686 0.1189 0.1705 0.1345 0.152 0.1834 0.3549 0.3114 0.22 0.3411 0.385
Cytoplasmic Foci 0.0082 0.0032 0.0056 0.0214 0.0094 0.0132 0.0259 0.0046 0.0141 0.004 0.0609 0.0093 0.0248 0.0107 0.0152 0.0061 0.0128 0.0084
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0004 0.0001 0.0001 0.0001 0.001 0.0001 0.0004 0 0.0004 0.0001 0.0002 0
Endoplasmic Reticulum 0.0018 0.0012 0.0033 0.0109 0.0011 0.0017 0.0246 0.0008 0.0019 0.002 0.0022 0.0012 0.0045 0.0054 0.0056 0.0042 0.0042 0.0018
Endosome 0.0022 0.001 0.0039 0.006 0.0034 0.0048 0.0307 0.0003 0.0098 0.0041 0.0201 0.0052 0.0158 0.0031 0.0036 0.0014 0.01 0.0028
Golgi 0.0007 0 0.0001 0.0004 0.002 0.0005 0.0095 0.0001 0.0037 0.0023 0.0426 0.003 0.0119 0.0002 0.0008 0.0001 0.0055 0
Lipid Particles 0.0021 0.0007 0.0005 0.0014 0.0032 0.003 0.0157 0.0005 0.0045 0.0006 0.0246 0.0011 0.0135 0.0008 0.0015 0.0003 0.0057 0.0001
Mitochondria 0.0004 0.0002 0.0005 0.0009 0.0464 0.0004 0.0039 0.0008 0.0257 0.0018 0.0533 0.0046 0.0036 0.0003 0.0028 0.0027 0.0015 0.0003
None 0.7775 0.7568 0.639 0.532 0.6182 0.5063 0.6523 0.8843 0.7072 0.6972 0.4355 0.7309 0.5735 0.5674 0.5184 0.6225 0.3641 0.389
Nuclear Periphery 0.0032 0.0038 0.0095 0.019 0.0118 0.0051 0.0173 0.0004 0.0041 0.0025 0.0007 0.0017 0.0084 0.0039 0.0066 0.0064 0.007 0.0048
Nucleolus 0.0009 0.0009 0.0013 0.0011 0.0005 0.0017 0.0028 0.0002 0.0007 0.0005 0.0008 0.0007 0.0005 0.0003 0.0047 0.0014 0.0129 0.0004
Nucleus 0.0361 0.0899 0.1398 0.1658 0.0472 0.1895 0.0384 0.0346 0.0696 0.0826 0.0023 0.0724 0.0603 0.0456 0.08 0.0852 0.1308 0.1892
Peroxisomes 0.0047 0.0001 0.0002 0.0015 0.0005 0.002 0.0183 0.0017 0.018 0.0007 0.1437 0.0015 0.0128 0.0002 0.0024 0.0005 0.0029 0.0001
Punctate Nuclear 0.0099 0.0096 0.023 0.0239 0.0388 0.0287 0.005 0.0013 0.0067 0.0025 0.0043 0.0108 0.0084 0.0042 0.0132 0.0043 0.0114 0.0161
Vacuole 0.0007 0.0005 0.0011 0.001 0.0009 0.0012 0.0064 0.0003 0.0025 0.0005 0.001 0.0007 0.0019 0.0015 0.002 0.0011 0.0085 0.0006
Vacuole Periphery 0.0001 0.0001 0.0003 0.0006 0.0049 0.0002 0.002 0.0001 0.0008 0.0002 0.0005 0.0002 0.0007 0.0002 0.0004 0.0003 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6223 1.3531 5.0201 6.9351 5.8873 7.5637 5.1303 3.2317 6.5068 9.1736
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1431 676 932 1518 1715 1196 1675 714 3146 1872 2607 2232

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 598.34 732.01 791.47 763.19 626.49 734.82 791.25 752.12 613.69 733.81 791.33 759.65
Standard Deviation 77.52 85.33 83.78 181.52 80.33 86.67 94.62 121.19 80.30 86.20 90.89 164.73
Intensity Change Log 2 — 0.290898 0.403569 0.351077 — 0.230099 0.336842 0.263671 — 0.260120 0.369825 0.307031

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000098 0.000482 0.000290 0.001283 0.000034 0.000164 0.000191 0.000303 0.000063 0.000279 0.000227 0.000970
Bud Neck 0.011150 0.031139 0.006390 0.010018 0.004529 0.013463 0.003477 0.003198 0.007541 0.019846 0.004519 0.007836
Bud Site 0.003404 0.013572 0.004357 0.025999 0.000864 0.004676 0.003078 0.005829 0.002019 0.007888 0.003535 0.019547
Cell Periphery 0.000155 0.000198 0.000143 0.000233 0.000105 0.000167 0.000130 0.000280 0.000128 0.000178 0.000135 0.000248
Cytoplasm 0.422345* 0.341196* 0.161054* 0.334798* 0.484808* 0.428018* 0.170169* 0.222311* 0.456396* 0.396666* 0.166910* 0.298814*
Cytoplasmic Foci 0.161615 0.237757* 0.002610 0.020294 0.112360 0.150576 0.004469 0.011680 0.134765 0.182058* 0.003805 0.017538
Eisosomes 0.000120 0.000158 0.000023 0.000057 0.000067 0.000096 0.000028 0.000044 0.000091 0.000118 0.000026 0.000052
Endoplasmic Reticulum 0.000697 0.001306 0.003854 0.002368 0.000620 0.000349 0.003438 0.002448 0.000655 0.000695 0.003587 0.002393
Endosome 0.006198 0.018583 0.000948 0.010144 0.003146 0.006724 0.001062 0.001298 0.004535 0.011007 0.001021 0.007314
Golgi 0.001544 0.005954 0.000051 0.003455 0.000495 0.000794 0.000053 0.001103 0.000972 0.002657 0.000053 0.002702
Lipid Particles 0.012152 0.008541 0.000688 0.003296 0.005411 0.004742 0.000866 0.001367 0.008477 0.006114 0.000803 0.002679
Mitochondria 0.004228 0.019533 0.001050 0.008858 0.002173 0.005418 0.000857 0.000600 0.003107 0.010515 0.000926 0.006216
Mitotic Spindle 0.000304 0.006892 0.002367 0.031264 0.000053 0.003732 0.003211 0.004113 0.000167 0.004873 0.002909 0.022579
None 0.015095 0.005890 0.005041 0.012355 0.008335 0.006246 0.006685 0.014617 0.011410 0.006118 0.006097 0.013078
Nuclear Periphery 0.000782 0.000703 0.002336 0.002243 0.000571 0.000486 0.001890 0.001487 0.000667 0.000564 0.002050 0.002001
Nuclear Periphery Foci 0.000481 0.000539 0.000386 0.001429 0.000284 0.000216 0.000549 0.000694 0.000374 0.000333 0.000491 0.001194
Nucleolus 0.003634 0.004200 0.000531 0.001419 0.001317 0.002396 0.000868 0.001320 0.002371 0.003047 0.000748 0.001388
Nucleus 0.328285* 0.228703* 0.788966* 0.460385* 0.356083* 0.340691* 0.779751* 0.705235* 0.343439* 0.300251* 0.783045* 0.538711*
Peroxisomes 0.006826 0.021653 0.000156 0.002710 0.001772 0.007438 0.000145 0.000313 0.004071 0.012571 0.000149 0.001943
Vacuole 0.020389 0.050715 0.018472 0.063375 0.016640 0.022731 0.018883 0.020922 0.018345 0.032837 0.018736 0.049794
Vacuole Periphery 0.000499 0.002286 0.000287 0.004018 0.000333 0.000877 0.000199 0.000837 0.000408 0.001386 0.000230 0.003000

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.01 -2.80 -3.44 -1.60 -2.25 -2.77 -13.84 -6.29 -3.14 -2.25 -5.62 -7.25 -4.32 -2.76 -2.74
Bud Neck -6.27 1.98 1.39 7.17 -0.88 -6.85 0.49 -0.35 6.21 -0.70 -8.43 2.59 -0.19 8.25 -2.74
Bud Site -5.35 -1.14 -5.87 -1.18 -5.15 -4.40 -4.17 -4.49 -1.62 -2.78 -6.41 -2.80 -7.52 -3.40 -6.31
Cell Periphery -2.48 0.71 -1.26 1.26 -1.42 -2.32 -2.72 -4.56 -2.31 -3.91 -2.77 -0.29 -5.16 -1.16 -4.41
Cytoplasm 6.41 29.08 14.04 4.78 -14.36 5.85 40.98 26.09 19.13 -4.78 7.65 49.51 27.29 16.65 -15.41
Cytoplasmic Foci -7.67 32.51 29.53 26.60 -12.14 -5.90 28.57 25.22 25.18 -4.53 -8.38 42.72 38.45 35.69 -11.59
Eisosomes -3.35 17.23 10.76 9.38 -12.51 -5.57 9.41 2.99 7.74 -4.94 -4.99 18.92 9.70 12.02 -12.36
Endoplasmic Reticulum -3.17 -12.10 -13.28 -9.30 3.77 3.43 -18.12 -12.50 -14.17 3.83 -0.51 -21.47 -18.22 -18.01 5.42
Endosome -6.92 8.80 0.31 6.76 -5.99 -3.77 6.09 4.64 5.67 -1.47 -7.14 10.63 0.06 6.43 -6.04
Golgi -3.36 2.94 -0.27 3.21 -3.31 -2.38 6.04 -0.44 0.04 -1.10 -3.36 3.92 -0.98 2.01 -3.40
Lipid Particles 2.66 10.67 8.49 6.56 -3.91 0.98 8.57 7.48 8.35 -3.37 3.41 13.40 10.04 8.31 -4.12
Mitochondria -5.22 5.24 -0.65 4.80 -3.98 -2.37 2.35 2.76 3.81 1.91 -5.37 5.32 -0.63 4.68 -3.99
Mitotic Spindle -3.38 -3.89 -8.31 -4.82 -7.03 -3.26 -3.61 -3.14 -1.34 -1.41 -4.65 -4.70 -8.89 -5.73 -6.71
None 4.37 5.41 3.33 -1.40 -2.60 1.85 2.36 -2.38 -3.55 -3.88 4.75 5.81 1.37 -3.11 -4.05
Nuclear Periphery 0.62 -14.24 -15.96 -15.42 -2.34 1.47 -20.71 -12.21 -12.78 4.37 1.66 -24.07 -20.08 -20.72 -2.04
Nuclear Periphery Foci -0.06 0.14 -3.31 -3.55 -3.54 1.52 -6.56 -2.45 -2.62 -1.13 1.14 -4.06 -4.57 -5.04 -2.97
Nucleolus -0.36 5.28 4.00 3.97 -3.04 -3.49 2.84 0.32 3.51 -2.11 -1.34 5.69 3.77 5.09 -2.81
Nucleus 8.78 -45.70 -15.73 -21.98 26.40 1.63 -49.29 -27.15 -26.79 6.80 5.76 -67.23 -26.15 -28.54 26.81
Peroxisomes -5.80 6.18 4.65 8.82 -3.77 -6.72 8.82 7.44 8.64 -2.81 -7.60 7.79 5.22 11.32 -4.00
Vacuole -8.27 -2.43 -20.00 -11.66 -17.38 -4.15 -7.57 -7.02 -5.37 -2.56 -8.75 -7.56 -21.08 -16.09 -16.30
Vacuole Periphery -5.46 2.19 -2.95 0.32 -3.35 -2.37 3.34 -0.90 0.48 -1.34 -5.19 4.06 -3.19 -0.59 -3.70
Standard name
Human Ortholog
Description Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
Localization
Cell Percentages cytoplasm (28%), nucleus (4%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Psy3

Psy3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psy3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available