Standard name | |
---|---|
Human Ortholog | |
Description | Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 |
Bud | 1 | 1 | 2 | 4 | 3 | 5 | 8 | 21 | 12 | – | 6 | 9 | 0 | 1 | 2 | 2 | 0 | 1 | 1 | 2 | 1 | 4 | 2 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 5 | 5 | 11 | 17 | 14 | – | 8 | 13 | 0 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 9 | 10 | 13 | 13 | 11 | 33 | 18 | 11 | – | 6 | 8 | 28 | 45 | 51 | 21 | 20 | 30 | 0 | 1 | 0 | 2 | 2 | 7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 14 | 6 | 8 | 0 | 0 | 0 | 0 | 2 | 3 |
Endosome | 71 | 38 | 25 | 11 | 99 | 22 | 44 | 28 | 14 | – | 3 | 6 | 30 | 51 | 34 | 96 | 54 | 62 | 84 | 114 | 121 | 85 | 105 | 131 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 |
Mitochondria | 13 | 57 | 125 | 201 | 140 | 305 | 368 | 380 | 367 | – | 221 | 262 | 59 | 44 | 106 | 30 | 10 | 25 | 7 | 7 | 16 | 12 | 15 | 15 |
Nucleus | 30 | 29 | 22 | 30 | 31 | 47 | 73 | 90 | 61 | – | 39 | 40 | 31 | 41 | 37 | 89 | 34 | 57 | 13 | 24 | 9 | 17 | 11 | 7 |
Nuclear Periphery | 29 | 55 | 29 | 49 | 13 | 41 | 84 | 57 | 68 | – | 51 | 53 | 30 | 27 | 31 | 63 | 42 | 70 | 39 | 57 | 55 | 23 | 40 | 41 |
Nucleolus | 56 | 47 | 30 | 22 | 39 | 76 | 71 | 63 | 86 | – | 58 | 59 | 34 | 28 | 42 | 109 | 56 | 77 | 18 | 24 | 18 | 19 | 14 | 13 |
Peroxisomes | 1 | 0 | 3 | 2 | 19 | 2 | 12 | 5 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 3 | 5 | 8 | 4 | 4 | 5 |
SpindlePole | 38 | 17 | 43 | 13 | 44 | 25 | 40 | 45 | 18 | – | 5 | 11 | 12 | 22 | 19 | 60 | 35 | 24 | 49 | 43 | 60 | 48 | 42 | 53 |
Vac/Vac Membrane | 19 | 47 | 9 | 10 | 13 | 11 | 16 | 10 | 11 | – | 4 | 8 | 13 | 19 | 32 | 32 | 28 | 23 | 5 | 16 | 9 | 10 | 12 | 8 |
Unique Cell Count | 163 | 220 | 233 | 270 | 303 | 389 | 516 | 534 | 443 | 278 | 307 | 174 | 221 | 278 | 365 | 194 | 269 | 233 | 309 | 318 | 247 | 267 | 311 | |
Labelled Cell Count | 263 | 300 | 299 | 359 | 419 | 551 | 761 | 737 | 662 | 402 | 471 | 237 | 278 | 355 | 520 | 289 | 379 | 233 | 309 | 318 | 247 | 267 | 311 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.9 | 3.9 | 4.0 | 4.3 | 3.9 | 4.0 | 4.0 | 3.5 | – | 3.5 | 3.5 | 4.5 | 4.9 | 4.6 | 6.4 | 7.3 | 7.1 | 5.2 | 5.2 | 5.6 |
Std Deviation (1e-4) | 0.9 | 1.0 | 0.6 | 1.3 | 1.2 | 0.9 | 1.2 | 1.3 | 1.1 | – | 0.8 | 1.0 | 1.0 | 1.3 | 1.5 | 1.4 | 1.7 | 1.6 | 1.3 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.12 | 0.0 | 0.02 | 0.02 | -0.17 | – | -0.16 | -0.18 | 0.19 | 0.31 | 0.21 | 0.71 | 0.9 | 0.84 | 0.4 | 0.39 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 0 | -1.0 | 1.1 | -0.6 | -1.3 | – | -1.4 | -1.1 | 4.0 | 5.2 | 4.9 | 0.8 | 2.4 | 2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.6 | 4.5 | 3.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -12.4 | -10.7 | -10.8 |
Nucleus | 0.6 | 0.3 | 1.0 | 1.8 | 2.7 | 1.6 | – | 1.6 | 1.3 | 2.5 | 2.8 | 1.4 | 4.6 | 2.5 | 3.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.5 | 3.8 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.8 | 4.1 | 4.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.1 | -3.1 |
Vacuole | -0.1 | 0.2 | -0.7 | -0.5 | -1.6 | -1.0 | – | -1.7 | -0.8 | 1.6 | 2.1 | 3.2 | 2.3 | 3.9 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9332 | 2.2723 | 1.9648 | 2.0469 | 2.5425 | 2.1931 | 3.2127 | 3.7927 | 3.3079 | 3.4607 | 2.972 | 3.2078 | 4.2209 | 4.0154 | 3.7233 | 3.1956 | 3.6316 | 3.5992 |
Actin | 0.0187 | 0.0014 | 0.0103 | 0.0314 | 0.0717 | 0.0022 | 0.0107 | 0.0009 | 0.0063 | 0.0048 | 0.0036 | 0.0008 | 0.004 | 0.0037 | 0.0066 | 0.0007 | 0.004 | 0.0018 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0.0016 | 0.0016 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Bud Neck | 0.0012 | 0.0005 | 0.0009 | 0.0024 | 0.0064 | 0.0017 | 0.0008 | 0.0004 | 0.0003 | 0.0012 | 0.0022 | 0.001 | 0.0008 | 0.0008 | 0.0005 | 0.0003 | 0.0004 | 0.0025 |
Bud Periphery | 0.0006 | 0.0003 | 0.0003 | 0.0046 | 0.0032 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0013 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0003 |
Bud Site | 0.0009 | 0.0008 | 0.0006 | 0.0023 | 0.0131 | 0.0002 | 0.0016 | 0.0002 | 0.0002 | 0.0072 | 0.0033 | 0 | 0.0008 | 0.0008 | 0.0013 | 0.0003 | 0.0001 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0195 | 0.0017 | 0.0031 | 0.0015 | 0.0023 | 0.001 | 0.0042 | 0.0017 | 0.0009 | 0.0062 | 0.0016 | 0.0003 | 0.0008 | 0.0021 | 0.0014 | 0.0003 | 0.011 | 0.0007 |
Cytoplasmic Foci | 0.0116 | 0.0064 | 0.0118 | 0.0064 | 0.0106 | 0.0024 | 0.0101 | 0.0027 | 0.0046 | 0.0108 | 0.0173 | 0.001 | 0.0192 | 0.0044 | 0.0045 | 0.0035 | 0.0038 | 0.0041 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0009 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0042 | 0.0024 | 0.0025 | 0.0038 | 0.0028 | 0.0007 | 0.0029 | 0.001 | 0.0014 | 0.0015 | 0.0013 | 0.0001 | 0.0011 | 0.0014 | 0.0018 | 0.0002 | 0.0028 | 0.0004 |
Endosome | 0.0175 | 0.0076 | 0.015 | 0.0123 | 0.0221 | 0.0037 | 0.0211 | 0.0031 | 0.0055 | 0.0084 | 0.0314 | 0.0013 | 0.0101 | 0.0047 | 0.0065 | 0.0015 | 0.0073 | 0.0027 |
Golgi | 0.0077 | 0.0026 | 0.0043 | 0.0066 | 0.0152 | 0.0021 | 0.0125 | 0.0014 | 0.0028 | 0.0024 | 0.012 | 0.0007 | 0.0086 | 0.0018 | 0.0028 | 0.001 | 0.0021 | 0.002 |
Lipid Particles | 0.086 | 0.0462 | 0.0605 | 0.0624 | 0.069 | 0.0235 | 0.0373 | 0.0209 | 0.0267 | 0.0132 | 0.0395 | 0.0112 | 0.0935 | 0.0157 | 0.0309 | 0.009 | 0.0384 | 0.0285 |
Mitochondria | 0.002 | 0.001 | 0.0022 | 0.0034 | 0.0092 | 0.0005 | 0.0047 | 0.0004 | 0.0018 | 0.0017 | 0.0021 | 0.0002 | 0.0023 | 0.0006 | 0.0101 | 0.0001 | 0.0012 | 0.0004 |
None | 0.0019 | 0.0021 | 0.0051 | 0.0016 | 0.0023 | 0.0006 | 0.0091 | 0.0011 | 0.0038 | 0.003 | 0.0012 | 0.0003 | 0.0009 | 0.004 | 0.0178 | 0.0005 | 0.0055 | 0.0008 |
Nuclear Periphery | 0.4845 | 0.4832 | 0.4567 | 0.2627 | 0.3229 | 0.487 | 0.5733 | 0.5361 | 0.5869 | 0.447 | 0.5146 | 0.4799 | 0.4219 | 0.3711 | 0.5775 | 0.382 | 0.6252 | 0.4582 |
Nucleolus | 0.0245 | 0.034 | 0.0291 | 0.0573 | 0.052 | 0.0646 | 0.0132 | 0.0346 | 0.0149 | 0.021 | 0.0257 | 0.0646 | 0.0162 | 0.0319 | 0.0229 | 0.0484 | 0.0064 | 0.1142 |
Nucleus | 0.0905 | 0.1297 | 0.0844 | 0.1124 | 0.0911 | 0.1064 | 0.0354 | 0.1304 | 0.0548 | 0.0722 | 0.0339 | 0.0968 | 0.065 | 0.0832 | 0.0574 | 0.0245 | 0.1111 | 0.1134 |
Peroxisomes | 0.0059 | 0.0024 | 0.0089 | 0.0096 | 0.0079 | 0.001 | 0.0103 | 0.0009 | 0.0025 | 0.0028 | 0.0055 | 0.0005 | 0.0174 | 0.0022 | 0.0018 | 0.0008 | 0.0025 | 0.0036 |
Punctate Nuclear | 0.2159 | 0.27 | 0.2931 | 0.4111 | 0.2901 | 0.2991 | 0.2436 | 0.2587 | 0.2812 | 0.3905 | 0.3008 | 0.3394 | 0.3308 | 0.4667 | 0.2436 | 0.5244 | 0.1729 | 0.2635 |
Vacuole | 0.0015 | 0.0022 | 0.0037 | 0.0022 | 0.0022 | 0.0005 | 0.0025 | 0.0006 | 0.0005 | 0.0011 | 0.0012 | 0.0002 | 0.0009 | 0.0007 | 0.0023 | 0.0001 | 0.0007 | 0.0004 |
Vacuole Periphery | 0.0046 | 0.0054 | 0.007 | 0.0035 | 0.0029 | 0.0025 | 0.006 | 0.0047 | 0.0045 | 0.0029 | 0.0023 | 0.0015 | 0.0051 | 0.0035 | 0.0087 | 0.0022 | 0.0044 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.895 | 6.3811 | 12.7098 | 12.0159 | 12.1775 | 11.2645 | 10.5267 | 10.3135 | 11.5447 | 13.7818 |
Translational Efficiency | 1.1351 | 1.6113 | 0.6667 | 0.8492 | 0.7196 | 1.0541 | 0.938 | 0.9532 | 0.8462 | 0.8558 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|