Standard name
Human Ortholog
Description Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.12 0.06 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.09 0 0 0 0.2 0.1 0.1 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.94 0.94 0.86 0.87 0.83 0.83 0.72 0.75 0.77 0.74 0.9 0.86 0.81 0.88 0.92 0.89 0.9 0.93 0.88 0.65 0.64 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.06 0 0.09 0.1 0.16 0.26 0.23 0.45 0.38 0.41 0.35 0.11 0.09 0.12 0 0 0 0 0 0.05 0.16 0.17 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 0 0 1 1 2 2 4 0 0 0 1 0 0 9 2 0 2 1 2
Bud Neck 0 0 0 0 1 1 2 2 3 4 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 3 2 3 5 16 10 12 20 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 8 10 13 16 12 10 3 6 3 4 9 20 29 9 7 8 0 1 0 5 7 6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 0 2
Endosome 1 2 0 0 1 0 1 1 0 0 0 1 0 0 0 1 0 0 0 2 2 2 1 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1
Mitochondria 6 24 2 1 28 13 21 13 114 52 59 102 1 0 1 4 2 2 1 5 0 2 2 5
Nucleus 402 481 282 250 279 452 456 471 400 386 446 473 151 177 194 133 116 109 392 501 283 101 166 116
Nuclear Periphery 1 6 0 0 1 6 4 4 4 9 8 2 0 1 1 1 0 0 0 1 1 1 2 4
Nucleolus 48 32 13 23 34 84 140 128 251 195 241 222 18 18 29 6 3 4 14 8 16 25 43 61
Peroxisomes 1 0 0 0 0 0 0 1 0 1 1 0 0 0 0 2 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 4 6 0 5 2 3 1 3 5 0 0 0 4 1 1 2 5 3
Vac/Vac Membrane 4 2 1 1 4 1 2 8 1 2 3 2 1 0 2 2 2 3 9 8 10 5 22 29
Unique Cell Count 424 512 299 267 324 522 548 567 558 517 582 639 167 205 240 151 126 123 435 537 320 156 261 245
Labelled Cell Count 467 551 307 286 366 576 645 650 793 672 779 835 181 219 261 161 130 130 435 537 320 156 261 245


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 7.6 9.8 8.9 7.2 7.8 7.4 7.8 5.9 6.5 6.4 6.3 9.3 8.9 8.5 8.7 11.1 11.2 9.2 10.0 10.3
Std Deviation (1e-4) 2.6 1.6 1.8 1.9 1.9 1.7 1.7 1.6 1.3 1.5 1.4 1.5 2.8 2.8 3.5 2.2 2.5 2.8 2.2 2.7 2.3
Intensity Change (Log2) – – – -0.12 -0.44 -0.32 -0.4 -0.33 -0.72 -0.58 -0.61 -0.64 -0.08 -0.13 -0.19 -0.17 0.18 0.19 -0.09 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.0 0 0 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.9 0.3 -0.4 -0.9 0 -1.6 0 0 1.5 3.4 4.3 1.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 1.9 2.7 1.7 8.1 5.2 5.2 7.0 0 0 0 0 0 0
Nucleus -0.3 -3.4 -3.5 -4.6 -4.7 -7.8 -7.0 -6.6 -7.3 -1.6 -3.1 -4.8 -2.3 -0.9 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 2.9 5.0 7.7 6.9 12.3 10.5 11.5 10.0 2.7 2.0 3.3 -0.2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0